FastQCFastQC Report
Sat 18 Jun 2016
SRR3552136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471498
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174063.6916381405647534No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG72521.538076513580121No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC64751.3732826014108226No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC55831.1840983418805593No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC15960.33849560337477574No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT14170.30053149748249197No Hit
GAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT13480.28589728906591333No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTC11930.25302334262287435No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTA11740.2489936330588889No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC8640.18324574017281092No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG253.887294E-545.01
TTGTGCG207.029049E-445.01
CGCATCT207.029049E-445.045
ACGGGTC502.1827873E-1145.05
CTAACGG207.029049E-445.02
TGCGCAT207.029049E-445.043
ACTACGG253.887294E-545.02
GTACTCC207.029049E-445.042
TACTCCA207.029049E-445.043
TCCCGAT207.029049E-445.039
CGTAAGG453.8380676E-1045.02
CGATATT207.029049E-445.033
TACGTTG253.887294E-545.01
CTTACGA207.029049E-445.029
CCCGATG207.029049E-445.040
TACCGGG302.1627675E-644.9999963
CGTTTTT87300.043.144331
TAGGGTA1100.042.9545445
TACGGCT7600.041.1513147
GCTCGTA556.002665E-1140.90909232