##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471498
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60239067822133	31.0	30.0	33.0	28.0	34.0
2	30.533262071101046	31.0	30.0	33.0	25.0	34.0
3	30.151871269867527	31.0	30.0	34.0	25.0	34.0
4	34.48115792643871	35.0	33.0	37.0	32.0	37.0
5	35.14818726696614	35.0	35.0	37.0	33.0	37.0
6	35.08573313142367	35.0	35.0	37.0	32.0	37.0
7	35.58267479395459	37.0	35.0	37.0	35.0	37.0
8	35.19394143771554	37.0	35.0	37.0	32.0	37.0
9	37.238942689046404	39.0	37.0	39.0	34.0	39.0
10	36.701139771536674	39.0	35.0	39.0	32.0	39.0
11	36.91763909921145	39.0	37.0	39.0	33.0	39.0
12	37.11719667951932	39.0	37.0	39.0	34.0	39.0
13	37.04224408162919	39.0	37.0	39.0	33.0	39.0
14	38.22301473176981	40.0	38.0	41.0	33.0	41.0
15	38.34702162045226	40.0	38.0	41.0	34.0	41.0
16	38.20283649135309	40.0	37.0	41.0	34.0	41.0
17	37.87328260141082	40.0	37.0	41.0	33.0	41.0
18	37.62905463013629	39.0	37.0	40.0	33.0	41.0
19	36.974884304917516	38.0	36.0	40.0	32.0	41.0
20	36.670240382779994	38.0	35.0	40.0	32.0	41.0
21	36.89727421961493	38.0	35.0	40.0	33.0	41.0
22	37.191650865963375	39.0	35.0	40.0	33.0	41.0
23	37.23690874616647	39.0	35.0	40.0	33.0	41.0
24	37.16134745004221	39.0	35.0	41.0	33.0	41.0
25	36.68963813208116	38.0	35.0	40.0	32.0	41.0
26	36.797070189057	38.0	35.0	40.0	33.0	41.0
27	37.01918990112365	38.0	35.0	40.0	33.0	41.0
28	36.8968373142622	39.0	35.0	41.0	33.0	41.0
29	36.8143936983826	39.0	35.0	41.0	33.0	41.0
30	36.469036984250195	38.0	35.0	41.0	32.0	41.0
31	36.30256331946265	39.0	35.0	41.0	31.0	41.0
32	35.77615175462038	38.0	35.0	41.0	29.0	41.0
33	35.207812122214726	39.0	35.0	41.0	22.0	41.0
34	34.714461142995304	39.0	35.0	41.0	18.0	41.0
35	34.36943104742756	39.0	35.0	41.0	15.0	41.0
36	34.15232726331819	39.0	34.0	41.0	15.0	41.0
37	33.72400307106287	38.0	34.0	41.0	10.0	41.0
38	33.70650776885586	38.0	34.0	41.0	10.0	41.0
39	33.620946430313595	38.0	33.0	41.0	10.0	41.0
40	33.543896262550426	38.0	33.0	41.0	10.0	41.0
41	33.43186821577186	38.0	33.0	40.0	10.0	41.0
42	33.24102117082151	38.0	33.0	40.0	10.0	41.0
43	33.26739880126745	38.0	33.0	40.0	10.0	41.0
44	33.26069463709284	38.0	33.0	40.0	10.0	41.0
45	33.19499764580125	38.0	33.0	40.0	10.0	41.0
46	33.05695464243751	38.0	33.0	40.0	10.0	41.0
47	33.017355322822155	37.0	32.0	40.0	10.0	41.0
48	33.02469999872746	37.0	33.0	40.0	10.0	41.0
49	33.00305621656932	37.0	33.0	40.0	10.0	41.0
50	32.91469953212951	37.0	32.0	40.0	10.0	41.0
51	31.716454788779593	35.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	11.0
10	14.0
11	5.0
12	18.0
13	9.0
14	21.0
15	27.0
16	62.0
17	112.0
18	297.0
19	592.0
20	889.0
21	1344.0
22	2010.0
23	3078.0
24	4812.0
25	8179.0
26	13187.0
27	15971.0
28	15730.0
29	13751.0
30	12610.0
31	12986.0
32	14049.0
33	18162.0
34	28662.0
35	33170.0
36	38913.0
37	55818.0
38	100248.0
39	76745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.06951461087852	14.88808011910973	16.791587663150214	30.250817606861535
2	40.490097518971446	25.841891163907377	19.34133336726773	14.326677949853444
3	25.003499484621354	24.480061421257354	38.65021696804652	11.866222126074765
4	22.383764088076727	19.863710980746472	43.787460392196785	13.965064538980018
5	23.15874086422424	23.027669258406185	37.714051809339594	16.099538068029982
6	19.159572256934283	31.784440230923565	38.34904920063288	10.706938311509274
7	60.00958646696274	5.726429380400341	30.55495463395391	3.709029518683006
8	59.607675960449455	9.224429371916742	27.044229243814392	4.123665423819401
9	54.579022604549756	7.266838883728033	28.21178456748491	9.942353944237304
10	30.74286635362186	20.872835091559246	35.37936534195267	13.004933212866227
11	24.548354393868056	20.725432557508196	39.97683977450593	14.749373274117811
12	21.435297710700787	19.818535815634426	42.42796363929433	16.318202834370453
13	18.72118227436808	21.389486275657585	46.12638865912474	13.76294279084959
14	16.719477070952582	24.841886922107832	42.128068411742994	16.310567595196588
15	15.687447242618207	21.1977569364027	48.424171470504646	14.690624350474446
16	17.342597423530957	22.91059559107356	42.41735914044174	17.329447844953744
17	17.236552435005027	21.81663548944004	43.76794811430801	17.178863961246922
18	17.45330839155203	21.618331360896548	44.39658280628974	16.53177744126168
19	17.035066956805757	23.51356739583201	42.17281939690094	17.278546250461297
20	18.365083202897996	22.74601376888131	44.64515225939453	14.243750768826166
21	17.429342224145174	26.11294215457966	42.10282970447383	14.354885916801344
22	17.02679544770073	21.487259755078494	42.4073909115203	19.07855388570047
23	16.48639018617258	25.077518886612456	42.690955210838645	15.745135716376316
24	17.44397643256175	21.915257328769158	42.408663451382616	18.23210278728648
25	15.719684919130092	26.66034638535052	41.33930578708711	16.280662908432273
26	16.364862629321863	22.736681809891028	42.964763371212605	17.933692189574504
27	17.052458334924008	24.137536108318596	40.97514729648906	17.834858260268334
28	14.431450398517068	23.61049251534471	44.31810951478055	17.639947571357673
29	18.887672906353792	22.418971024267336	41.08140437499205	17.611951694386825
30	16.402614645237097	23.518021285350095	42.559883605020595	17.519480464392213
31	18.72139436434513	22.769131576379962	39.16708024212191	19.34239381715299
32	18.594352468091063	26.53945509843096	38.67481940538454	16.19137302809344
33	16.181828979126102	24.53202346563506	38.42200815273872	20.86413940250012
34	18.554903732359413	25.80010943842816	36.943953102664274	18.70103372654815
35	16.410674064365065	28.08707566097841	35.286682021981	20.215568252675514
36	18.62001535531434	29.10256247110274	33.6198668923304	18.65755528125252
37	17.114388608223152	29.458025272641663	34.64892746098605	18.778658658149133
38	16.378860567807287	28.714013633143725	34.77575726726306	20.131368531785927
39	18.475794170919073	27.790785963036956	34.699829055478496	19.033590810565475
40	18.066036335254868	27.03447310487001	37.11235254444345	17.787138015431665
41	16.372709958472782	29.638089663158702	33.000139979384855	20.989060398983664
42	17.703150384519127	27.914434419658196	35.9634611387535	18.418954057069172
43	18.83846803167776	27.123338805254743	34.02601919838472	20.012173964682777
44	18.189260611922002	26.07116042910044	35.09686149251959	20.64271746645797
45	17.104632469278766	25.956843931469486	35.468443132314455	21.470080466937294
46	19.525215377371698	27.701708172675175	34.173209642458716	18.59986680749441
47	16.111415106744886	25.99904983690281	39.69179084534823	18.19774421100408
48	18.05734064619574	26.646136356888046	35.62772270508041	19.668800291835808
49	17.10145111962299	24.568927121642087	38.88966655213808	19.439955206596846
50	17.069001353134052	25.048250469779298	37.28881140535061	20.59393677173604
51	17.008343619697218	24.608800037327835	35.269290643862746	23.11356569911219
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	478.0
1	1398.0
2	2318.0
3	17605.5
4	32893.0
5	21403.0
6	9913.0
7	9254.0
8	8595.0
9	8369.5
10	8144.0
11	7864.5
12	7585.0
13	7027.5
14	6470.0
15	6290.5
16	6111.0
17	5798.5
18	5486.0
19	5068.0
20	4650.0
21	4429.0
22	4208.0
23	4416.0
24	4624.0
25	4870.0
26	5715.0
27	6314.0
28	7257.5
29	8201.0
30	9242.5
31	10284.0
32	11121.0
33	11958.0
34	12925.5
35	13893.0
36	14460.5
37	15028.0
38	16505.5
39	17983.0
40	19610.0
41	21237.0
42	22935.0
43	24633.0
44	26087.5
45	27542.0
46	41059.0
47	54576.0
48	43029.0
49	31482.0
50	31048.0
51	30614.0
52	27124.0
53	23634.0
54	21023.0
55	18412.0
56	15952.0
57	13492.0
58	11704.5
59	9917.0
60	8577.0
61	7237.0
62	6442.0
63	5647.0
64	4750.0
65	3853.0
66	3250.0
67	2647.0
68	2297.0
69	1947.0
70	1665.0
71	1383.0
72	1214.0
73	1045.0
74	901.5
75	635.5
76	513.0
77	417.0
78	321.0
79	244.0
80	167.0
81	134.5
82	102.0
83	74.5
84	47.0
85	30.5
86	14.0
87	12.5
88	11.0
89	8.5
90	6.0
91	6.0
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	471498.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.33281498450291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78574483769466	30.59532310132797
2	8.781868170444529	6.908311919327795
3	3.545740562040043	4.183918725293051
4	1.938838980073341	3.0503997955186812
5	1.2948717467738342	2.546547542225766
6	0.8992225131940116	2.1221371644815874
7	0.6329015691416753	1.7425660225715764
8	0.5324270400684757	1.6753483407807899
9	0.46188974768807745	1.6350681590148495
>10	3.957568227470561	29.749671957512163
>50	0.12285571302600568	3.081645036472928
>100	0.040586262338948215	3.1506773369049377
>500	5.484630045803812E-4	0.1863872111664407
>1k	0.0027423150229019064	1.4514041165831169
>5k	0.0016453890137411438	4.165667879194409
>10k+	5.484630045803812E-4	3.75492569162392
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17406	3.6916381405647534	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG	7252	1.538076513580121	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	6475	1.3732826014108226	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC	5583	1.1840983418805593	No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	1596	0.33849560337477574	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT	1417	0.30053149748249197	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT	1348	0.28589728906591333	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTC	1193	0.25302334262287435	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTA	1174	0.2489936330588889	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC	864	0.18324574017281092	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07317104208289325	0.0
2	0.0	0.0	0.0	0.5874892364336646	0.0
3	0.0	0.0	0.0	0.7128344128713165	0.0
4	0.0	0.0	0.0	1.1365901870209418	0.0
5	0.0	0.0	0.0	2.9539892003783685	0.0
6	0.0	0.0	0.0	3.3800779642755643	0.0
7	0.0	0.0	0.0	4.034375543480566	0.0
8	0.0	0.0	0.0	4.80723141985756	0.0
9	0.0	0.0	0.0	5.063011932182109	0.0
10	0.0	0.0	0.0	7.190698581966413	0.0
11	0.0	0.0	0.0	7.970553427586119	0.0
12	0.0	0.0	0.0	9.88848309006613	0.0
13	0.0	0.0	0.0	10.221252264060505	0.0
14	0.0	0.0	0.0	10.382864826574027	0.0
15	0.0	0.0	0.0	10.824224068818955	0.0
16	0.0	0.0	0.0	11.218499336158372	0.0
17	0.0	0.0	0.0	11.626348362029107	0.0
18	0.0	0.0	0.0	12.12942578759613	0.0
19	0.0	0.0	0.0	12.51394491599116	0.0
20	0.0	0.0	0.0	12.810446703909667	0.0
21	0.0	0.0	0.0	13.149790667192649	0.0
22	0.0	0.0	0.0	13.51267661792839	0.0
23	0.0	0.0	0.0	13.879804368205168	0.0
24	0.0	0.0	0.0	14.179911685733556	0.0
25	0.0	0.0	0.0	14.451811036314046	0.0
26	0.0	0.0	0.0	14.727528006481469	0.0
27	0.0	0.0	0.0	15.006002146350568	0.0
28	0.0	0.0	0.0	15.295504965026362	0.0
29	0.0	0.0	0.0	15.596460642462958	0.0
30	0.0	0.0	0.0	15.94810582441495	0.0
31	0.0	0.0	0.0	16.25160658157617	0.0
32	0.0	0.0	0.0	16.56274257791125	0.0
33	0.0	0.0	0.0	16.890845772410486	0.0
34	0.0	0.0	0.0	17.205587298355454	0.0
35	0.0	0.0	0.0	17.521813454139785	0.0
36	0.0	0.0	0.0	17.850340828593122	0.0
37	0.0	0.0	0.0	18.17441431352837	0.0
38	0.0	0.0	0.0	18.550237752864277	0.0
39	0.0	0.0	0.0	18.964449478046568	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.887294E-5	45.0	1
TTGTGCG	20	7.029049E-4	45.0	1
CGCATCT	20	7.029049E-4	45.0	45
ACGGGTC	50	2.1827873E-11	45.0	5
CTAACGG	20	7.029049E-4	45.0	2
TGCGCAT	20	7.029049E-4	45.0	43
ACTACGG	25	3.887294E-5	45.0	2
GTACTCC	20	7.029049E-4	45.0	42
TACTCCA	20	7.029049E-4	45.0	43
TCCCGAT	20	7.029049E-4	45.0	39
CGTAAGG	45	3.8380676E-10	45.0	2
CGATATT	20	7.029049E-4	45.0	33
TACGTTG	25	3.887294E-5	45.0	1
CTTACGA	20	7.029049E-4	45.0	29
CCCGATG	20	7.029049E-4	45.0	40
TACCGGG	30	2.1627675E-6	44.999996	3
CGTTTTT	8730	0.0	43.14433	1
TAGGGTA	110	0.0	42.954544	5
TACGGCT	760	0.0	41.151314	7
GCTCGTA	55	6.002665E-11	40.909092	32
>>END_MODULE