FastQCFastQC Report
Sat 18 Jun 2016
SRR3552134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331082
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203976.160709431500353No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG90882.7449393201684176No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC84692.5579765737793054No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC75252.272850834536459No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTA20650.623712554593726No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT20590.6219003147256571No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC19770.5971330365287149No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT17180.5189046822237391No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTC14980.4524558870612114No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC11790.3561051340755462No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGATCGT7610.22985242326674357No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGT5550.16763218779637673No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT5450.16461178801626183No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC5230.15796690850000905No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATG5080.15343630882983672No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT4660.14075062975335417No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTAT4620.1395424698413082No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC4440.1341057502371014No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT4110.12413843096272222No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3530.10662011223805583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGG302.160803E-645.0000042
TCAAGCG302.160803E-645.00000417
ACGCGAT207.0260034E-445.026
TAGACGG207.0260034E-445.02
CGCGATT207.0260034E-445.027
TACGGTC207.0260034E-445.040
TAACGGG207.0260034E-445.03
ACGGGGT351.2084274E-745.05
ATCGTTG207.0260034E-445.023
AGTATGG207.0260034E-445.02
GAACGTA207.0260034E-445.020
GTTCCAT207.0260034E-445.029
GAGTTTA207.0260034E-445.041
ATGCGTT207.0260034E-445.012
CTACAGG351.2084274E-745.02
CGGCTAC207.0260034E-445.025
CATCCCC207.0260034E-445.016
AATACGG207.0260034E-445.02
CTTACTA207.0260034E-445.010
TGATCCG253.8847702E-544.9999964