##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	331082
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.711714922587152	31.0	30.0	33.0	28.0	34.0
2	30.76835043886409	31.0	30.0	33.0	27.0	34.0
3	30.37513667309005	31.0	30.0	34.0	25.0	34.0
4	34.487613340501746	35.0	35.0	37.0	32.0	37.0
5	35.178170362629196	35.0	35.0	37.0	33.0	37.0
6	35.13208812318398	36.0	35.0	37.0	33.0	37.0
7	35.56910070616947	37.0	35.0	37.0	35.0	37.0
8	35.046435626219484	37.0	35.0	37.0	32.0	37.0
9	37.060845953570414	39.0	37.0	39.0	32.0	39.0
10	36.82697035779656	39.0	37.0	39.0	32.0	39.0
11	37.05609184431651	39.0	37.0	39.0	33.0	39.0
12	37.21657172543358	39.0	37.0	39.0	34.0	39.0
13	37.05880416331906	39.0	37.0	39.0	33.0	39.0
14	38.27784959617255	40.0	38.0	41.0	33.0	41.0
15	38.23946635576685	40.0	38.0	41.0	33.0	41.0
16	38.256498390126914	40.0	37.0	41.0	34.0	41.0
17	37.80523858137863	40.0	37.0	41.0	32.0	41.0
18	37.41289167034149	39.0	37.0	40.0	33.0	41.0
19	36.67013005841453	37.0	36.0	39.0	32.0	41.0
20	35.95836982983067	36.0	35.0	40.0	32.0	41.0
21	36.2523695036275	36.0	35.0	40.0	32.0	41.0
22	36.57007025449889	37.0	35.0	40.0	33.0	41.0
23	36.62860258183773	37.0	35.0	40.0	33.0	41.0
24	36.48500975589129	37.0	35.0	40.0	33.0	41.0
25	36.10829039331646	36.0	35.0	40.0	32.0	41.0
26	36.20803003485541	36.0	35.0	40.0	33.0	41.0
27	36.41124253206154	37.0	35.0	40.0	33.0	41.0
28	36.205933877408015	37.0	35.0	40.0	33.0	41.0
29	36.128122338272696	37.0	35.0	40.0	32.0	41.0
30	35.66248240617128	36.0	35.0	40.0	31.0	41.0
31	35.37208606931213	37.0	35.0	40.0	29.0	41.0
32	34.643737805135885	37.0	35.0	40.0	22.0	41.0
33	33.604466567194834	37.0	33.0	40.0	16.0	41.0
34	32.84618614119765	37.0	33.0	40.0	10.0	41.0
35	32.33713702345643	37.0	31.0	40.0	8.0	41.0
36	31.998788819688173	37.0	31.0	40.0	8.0	41.0
37	31.609519696026965	37.0	29.0	40.0	8.0	41.0
38	31.550878634296037	36.0	29.0	40.0	8.0	41.0
39	31.444125624467656	36.0	27.0	40.0	7.0	41.0
40	31.265958282238238	36.0	26.0	40.0	7.0	41.0
41	31.11116883430691	36.0	25.0	40.0	7.0	41.0
42	30.942005303822015	36.0	24.0	40.0	7.0	41.0
43	30.94827565376553	35.0	24.0	40.0	7.0	41.0
44	30.91299134353423	35.0	24.0	40.0	7.0	41.0
45	30.84827927824527	35.0	23.0	40.0	7.0	41.0
46	30.641557076494646	35.0	23.0	40.0	7.0	41.0
47	30.520109821736003	35.0	23.0	40.0	7.0	41.0
48	30.548045499302287	35.0	23.0	40.0	7.0	41.0
49	30.509393443316156	35.0	24.0	40.0	7.0	41.0
50	30.383460894884045	35.0	23.0	40.0	7.0	41.0
51	29.021970388000558	35.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	6.0
12	7.0
13	14.0
14	13.0
15	32.0
16	53.0
17	136.0
18	336.0
19	532.0
20	793.0
21	1225.0
22	1975.0
23	2932.0
24	4859.0
25	8404.0
26	13442.0
27	16390.0
28	15584.0
29	13227.0
30	11620.0
31	11154.0
32	11278.0
33	13377.0
34	19937.0
35	25004.0
36	27943.0
37	35166.0
38	57450.0
39	38181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.66625186509686	10.634525585806538	13.344126228547609	37.35509632054899
2	48.45929407216339	26.89122332231894	14.190744286913818	10.458738318603851
3	19.123963247775475	26.628448541448947	45.10906663605995	9.138521574715629
4	16.78285137820842	14.762505965289566	56.50050440676328	11.954138249738735
5	23.34406582055201	17.383306854495263	45.38271485613836	13.889912468814373
6	15.1445865374741	29.468832494668995	46.82888227085737	8.557698696999534
7	46.10791284334395	4.674370699705813	45.497490047782726	3.7202264091675175
8	43.655952301846675	12.627989440682368	38.98097752218484	4.735080735286123
9	40.325357464313974	5.6572087881552005	39.36758869403954	14.649845053491282
10	26.545387547495785	15.887302843404353	44.97979352547103	12.587516083628831
11	22.147081387692474	16.015367794081225	48.436036993856504	13.401513824369793
12	17.491437166623374	15.218586332086915	51.28366990654884	16.00630659474088
13	16.585317232588906	17.039585359518185	55.248548697905655	11.126548709987254
14	14.440229308751306	21.87615152741617	48.36082903933165	15.322790124500878
15	11.631257513244453	16.873463371611866	58.112793809388606	13.38248530575507
16	12.980168055043764	18.912837303145444	49.328565128880456	18.77842951293033
17	13.223310237343014	17.868987139137737	52.99895494167608	15.908747681843169
18	13.641031526932904	18.23626775239971	52.22361831812059	15.899082402546801
19	13.756712838511307	19.71686772461203	49.75595169776671	16.770467739109947
20	14.414857950598343	19.45892558339022	54.37837152125455	11.747844944756888
21	13.638917247086823	24.290659111640018	49.37236092569213	12.698062715581035
22	12.560634525585806	17.29752750074	51.40931853740162	18.732519436272586
23	12.612887441781794	23.30782102319063	50.84359765858609	13.235693876441484
24	15.305573845754225	17.291486701179767	49.011121111990384	18.391818341075624
25	10.969789961399291	25.671887931086555	48.131278656042916	15.227043451471237
26	11.986456527385965	18.273418669695122	52.25895699554793	17.481167807370984
27	14.630514494898545	21.741139657245032	47.441117306286664	16.187228541569763
28	11.191789345237737	19.391570668293657	53.602128777765024	15.814511208703582
29	15.94318023933648	17.777771065778268	50.337076615460816	15.941972079424433
30	13.436550461819127	25.081399774074097	49.38323436490054	12.09881539920624
31	19.723814644106294	18.51656085199437	46.40874466144339	15.350879842455948
32	19.379791109151206	22.96289136830151	44.096024549809414	13.56129297273787
33	15.596438344579289	22.73213282510073	42.43752303054833	19.23390579977166
34	18.112733401393008	21.07332926586163	46.220271715164216	14.593665617581141
35	15.400716438827844	26.579216025033077	38.263632574407545	19.756434961731532
36	21.102325103750733	24.688445762681148	40.059864323641875	14.149364809926244
37	19.89144683190267	26.48951015156366	39.15404642958542	14.464996586948248
38	20.854652321781312	26.948308878163115	34.85571550250391	17.341323297551664
39	22.330419654345448	22.890401773578752	40.565177206855104	14.2140013652207
40	16.884940890776303	26.044605263952736	39.365172374215454	17.70528147105551
41	19.460737823258288	24.854567750587467	38.69615382292	16.988540603234245
42	18.672111440670285	21.635123624963	40.44889181532068	19.243873119046036
43	22.423750007551	22.806132619713544	36.480690584205725	18.28942678852973
44	18.557336249025923	22.585643435765157	39.98556248905105	18.87145782615787
45	17.28846630139965	21.246700213240224	39.31050313819537	22.15433034716475
46	21.944110522468755	24.473997378292992	37.530883587751674	16.051008511486582
47	14.79361608302475	22.11778350982536	46.370385584235926	16.71821482291396
48	18.116659921107157	23.727052512670575	39.19391570668294	18.962371859539328
49	16.97374064431168	20.310074241426594	44.34792589147099	18.368259222790726
50	17.838783141336588	20.610906059526037	41.210032559909635	20.340278239227743
51	16.99760180257459	20.66587733552413	38.11804930500601	24.21847155689527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	957.0
2	1604.0
3	19268.5
4	36933.0
5	23365.0
6	9797.0
7	9271.0
8	8745.0
9	8502.0
10	8259.0
11	7775.5
12	7292.0
13	6975.5
14	6659.0
15	6311.5
16	5964.0
17	5449.0
18	4934.0
19	4668.0
20	4402.0
21	4050.5
22	3699.0
23	3590.5
24	3482.0
25	3575.5
26	3519.5
27	3370.0
28	3845.5
29	4321.0
30	4607.0
31	4893.0
32	5404.0
33	5915.0
34	6216.5
35	6518.0
36	6918.0
37	7318.0
38	8013.0
39	8708.0
40	9588.5
41	10469.0
42	11820.0
43	13171.0
44	14489.5
45	15808.0
46	33779.0
47	51750.0
48	36863.0
49	21976.0
50	21453.0
51	20930.0
52	17731.0
53	14532.0
54	12420.5
55	10309.0
56	8738.5
57	7168.0
58	6187.0
59	5206.0
60	4551.5
61	3897.0
62	3374.0
63	2851.0
64	2463.5
65	2076.0
66	1743.5
67	1411.0
68	1116.0
69	821.0
70	720.5
71	620.0
72	551.5
73	483.0
74	390.0
75	255.5
76	214.0
77	175.5
78	137.0
79	109.5
80	82.0
81	59.5
82	37.0
83	30.5
84	24.0
85	17.0
86	10.0
87	7.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	331082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.84164412128581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64562872928298	35.79478598807853
2	8.085657420981686	7.089770302746255
3	3.019348456501288	3.9711960152424917
4	1.6125474025608204	2.827869174071012
5	1.0138240390536348	2.2223856360897014
6	0.7075375262088487	1.8611765055901965
7	0.5552421107794894	1.7039908915362147
8	0.4467441995456783	1.5668800167784258
9	0.38007963000162265	1.4996984288653015
>10	2.4009310516308404	17.758068987747265
>50	0.0804465669139415	2.406507821550096
>100	0.041610293231349044	3.3995153446306534
>500	0.0034675244359457535	0.8792950877982156
>1k	0.004161029323134905	3.1912452425760964
>5k	0.0020805146615674526	7.626030218587429
>10k+	6.935048871891508E-4	6.201584338112104
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20397	6.160709431500353	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG	9088	2.7449393201684176	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	8469	2.5579765737793054	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	7525	2.272850834536459	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTA	2065	0.623712554593726	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT	2059	0.6219003147256571	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	1977	0.5971330365287149	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	1718	0.5189046822237391	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTC	1498	0.4524558870612114	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	1179	0.3561051340755462	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGATCGT	761	0.22985242326674357	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGT	555	0.16763218779637673	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	545	0.16461178801626183	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	523	0.15796690850000905	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATG	508	0.15343630882983672	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT	466	0.14075062975335417	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTAT	462	0.1395424698413082	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	444	0.1341057502371014	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT	411	0.12413843096272222	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	353	0.10662011223805583	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.040799560229792E-4	0.0	0.0	0.18394234660899716	0.0
2	6.040799560229792E-4	0.0	0.0	1.0665031623585697	0.0
3	6.040799560229792E-4	0.0	0.0	1.2761189070985435	0.0
4	6.040799560229792E-4	0.0	0.0	2.060516729994382	0.0
5	6.040799560229792E-4	0.0	0.0	5.154010184788058	0.0
6	6.040799560229792E-4	0.0	0.0	5.649355748726902	0.0
7	6.040799560229792E-4	0.0	0.0	6.6962263125147246	0.0
8	6.040799560229792E-4	0.0	0.0	7.667888921777687	0.0
9	6.040799560229792E-4	0.0	0.0	7.929455542735637	0.0
10	6.040799560229792E-4	0.0	0.0	11.432817247690904	0.0
11	6.040799560229792E-4	0.0	0.0	12.54915700642137	0.0
12	6.040799560229792E-4	0.0	0.0	16.12380014618735	0.0
13	6.040799560229792E-4	0.0	0.0	16.599815151533456	0.0
14	6.040799560229792E-4	0.0	0.0	16.87406745156789	0.0
15	6.040799560229792E-4	0.0	0.0	17.82458726237005	0.0
16	6.040799560229792E-4	0.0	0.0	18.440446777535474	0.0
17	6.040799560229792E-4	0.0	0.0	19.009490096109122	0.0
18	6.040799560229792E-4	0.0	0.0	19.55286001655179	0.0
19	6.040799560229792E-4	0.0	0.0	20.16871953171722	0.0
20	6.040799560229792E-4	0.0	0.0	20.57073474245051	0.0
21	6.040799560229792E-4	0.0	0.0	20.98634175219432	0.0
22	6.040799560229792E-4	0.0	0.0	21.421581360508878	0.0
23	6.040799560229792E-4	0.0	0.0	21.79580889326511	0.0
24	6.040799560229792E-4	0.0	0.0	22.11536719000127	0.0
25	6.040799560229792E-4	0.0	0.0	22.40592964884832	0.0
26	6.040799560229792E-4	0.0	0.0	22.688034988311053	0.0
27	6.040799560229792E-4	0.0	0.0	22.998834125684876	0.0
28	6.040799560229792E-4	0.0	0.0	23.281241505125617	0.0
29	6.040799560229792E-4	0.0	0.0	23.56757540428051	0.0
30	6.040799560229792E-4	0.0	0.0	23.925492778224125	0.0
31	9.061199340344688E-4	0.0	0.0	24.205181797862764	0.0
32	9.061199340344688E-4	0.0	3.020399780114896E-4	24.51205441552244	0.0
33	9.061199340344688E-4	0.0	3.020399780114896E-4	24.814094393533928	0.0
34	9.061199340344688E-4	0.0	3.020399780114896E-4	25.092877293238534	0.0
35	9.061199340344688E-4	0.0	3.020399780114896E-4	25.413341709908725	0.0
36	9.061199340344688E-4	0.0	3.020399780114896E-4	25.707830688469926	0.0
37	9.061199340344688E-4	0.0	3.020399780114896E-4	25.985405428262485	0.0
38	9.061199340344688E-4	0.0	3.020399780114896E-4	26.300131085350458	0.0
39	9.061199340344688E-4	0.0	3.020399780114896E-4	26.644758700261566	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGG	30	2.160803E-6	45.000004	2
TCAAGCG	30	2.160803E-6	45.000004	17
ACGCGAT	20	7.0260034E-4	45.0	26
TAGACGG	20	7.0260034E-4	45.0	2
CGCGATT	20	7.0260034E-4	45.0	27
TACGGTC	20	7.0260034E-4	45.0	40
TAACGGG	20	7.0260034E-4	45.0	3
ACGGGGT	35	1.2084274E-7	45.0	5
ATCGTTG	20	7.0260034E-4	45.0	23
AGTATGG	20	7.0260034E-4	45.0	2
GAACGTA	20	7.0260034E-4	45.0	20
GTTCCAT	20	7.0260034E-4	45.0	29
GAGTTTA	20	7.0260034E-4	45.0	41
ATGCGTT	20	7.0260034E-4	45.0	12
CTACAGG	35	1.2084274E-7	45.0	2
CGGCTAC	20	7.0260034E-4	45.0	25
CATCCCC	20	7.0260034E-4	45.0	16
AATACGG	20	7.0260034E-4	45.0	2
CTTACTA	20	7.0260034E-4	45.0	10
TGATCCG	25	3.8847702E-5	44.999996	4
>>END_MODULE