##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257263
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.467401064280523	30.0	28.0	33.0	16.0	33.0
2	28.93841710622981	31.0	28.0	33.0	16.0	33.0
3	28.703218107539755	31.0	28.0	33.0	16.0	34.0
4	32.10161585614721	35.0	32.0	35.0	26.0	37.0
5	33.64145252134975	35.0	33.0	35.0	30.0	37.0
6	33.523336041327354	35.0	33.0	35.0	28.0	37.0
7	34.210640472979016	35.0	33.0	36.0	32.0	37.0
8	34.214041661645865	35.0	35.0	37.0	31.0	37.0
9	36.094801817595226	39.0	35.0	39.0	30.0	39.0
10	36.09812137773407	37.0	35.0	39.0	32.0	39.0
11	36.3439631816468	37.0	35.0	39.0	32.0	39.0
12	36.306425719983054	37.0	35.0	39.0	32.0	39.0
13	36.33006689652223	38.0	35.0	39.0	32.0	39.0
14	37.4961420802836	39.0	36.0	41.0	32.0	41.0
15	37.44207678523534	39.0	36.0	41.0	32.0	41.0
16	37.389927817058805	39.0	36.0	41.0	32.0	41.0
17	36.47789616073823	38.0	34.0	41.0	30.0	41.0
18	35.98974201498078	38.0	34.0	39.0	30.0	40.0
19	35.360592078923126	37.0	34.0	39.0	29.0	40.0
20	35.25381419014783	35.0	34.0	39.0	30.0	40.0
21	35.75457799994558	36.0	35.0	39.0	31.0	40.0
22	36.160543879220874	37.0	35.0	40.0	32.0	41.0
23	36.372863567633125	37.0	35.0	40.0	32.0	41.0
24	36.251159319451304	37.0	35.0	40.0	32.0	41.0
25	35.558723951753656	36.0	35.0	40.0	30.0	41.0
26	35.70164773014386	37.0	35.0	40.0	31.0	41.0
27	36.07887259341608	37.0	35.0	40.0	32.0	41.0
28	35.90818734135884	37.0	35.0	40.0	31.0	41.0
29	35.84751790968775	37.0	35.0	40.0	31.0	41.0
30	35.381967092042	37.0	35.0	40.0	29.0	41.0
31	35.095412088018875	37.0	35.0	40.0	27.0	41.0
32	34.5786141030774	37.0	34.0	40.0	23.0	41.0
33	33.76968316469916	37.0	33.0	40.0	18.0	41.0
34	33.09732452781784	37.0	33.0	40.0	12.0	41.0
35	32.59546456350117	37.0	32.0	40.0	10.0	41.0
36	32.252986243649495	37.0	31.0	40.0	8.0	41.0
37	32.20197618779226	37.0	31.0	40.0	8.0	41.0
38	31.91157686880741	37.0	31.0	40.0	8.0	41.0
39	31.786269304175104	37.0	30.0	40.0	8.0	41.0
40	31.367301166510536	36.0	29.0	40.0	8.0	41.0
41	31.22614989329985	36.0	27.0	40.0	7.0	41.0
42	31.23636512051869	36.0	27.0	40.0	7.0	41.0
43	31.24628881728037	36.0	28.0	40.0	7.0	41.0
44	31.217703284187778	36.0	28.0	40.0	7.0	41.0
45	31.174537341164488	36.0	27.0	40.0	7.0	41.0
46	31.012967274734415	36.0	27.0	40.0	7.0	41.0
47	30.969268802742718	35.0	27.0	40.0	7.0	41.0
48	30.949503037747363	35.0	27.0	40.0	7.0	41.0
49	30.920971923673438	35.0	27.0	40.0	7.0	41.0
50	30.841310254486654	35.0	26.0	40.0	7.0	41.0
51	29.18185281210279	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	14.0
11	9.0
12	6.0
13	10.0
14	21.0
15	33.0
16	99.0
17	188.0
18	405.0
19	622.0
20	927.0
21	1436.0
22	2050.0
23	3151.0
24	4838.0
25	7699.0
26	10804.0
27	11533.0
28	9911.0
29	8403.0
30	8077.0
31	8660.0
32	10281.0
33	13642.0
34	18028.0
35	24924.0
36	28330.0
37	38134.0
38	35549.0
39	9464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.270808472263795	11.568317247330553	14.97067203600984	36.19020224439581
2	44.871590551303534	27.17880145998453	16.06877009130734	11.880837897404602
3	19.897536761990647	26.24823624073419	42.776458332523525	11.077768664751636
4	18.67738462196274	16.312100846215742	52.763514380225686	12.247000151595838
5	23.734466285474397	18.803714486731476	42.38658493448339	15.075234293310736
6	16.471470829462458	29.85233010576725	44.08679056063251	9.58940850413779
7	51.37466328232199	4.363239175474125	40.561992979946595	3.700104562257301
8	49.03503418680494	10.795567182222083	34.94595025324279	5.223448377730183
9	45.497797973280264	5.406140797549589	34.92146169484147	14.174599534328683
10	28.47436281159747	16.298107384272125	41.62627350221369	13.601256301916715
11	22.127939112892253	16.733070826352797	45.32093616260403	15.818053898150918
12	18.46903752191337	15.286691051569795	49.16952690437412	17.07474452214271
13	17.154818221042277	16.59741198695498	54.010876029588395	12.236893762414338
14	15.634195356502879	21.982562591589154	46.14849395365832	16.234748098249653
15	12.807904751169035	17.655861900078907	55.480189533667875	14.056043815084173
16	14.712181697329193	19.175318642789673	46.20174685049929	19.910752809381837
17	15.680062815095836	17.904245849578057	48.36762379354979	18.048067541776312
18	15.356658361287865	18.676218500134105	48.60007074472427	17.36705239385376
19	15.02470234740324	20.615090393877082	45.437937052743685	18.922270205975984
20	15.61631482179715	20.42540124308587	49.832272810314734	14.126011124802245
21	15.896961475221854	22.718385465457526	46.941845504406	14.442807554914621
22	14.127954661183303	17.72893886800667	47.191784283009994	20.951322187800034
23	14.676420627917732	22.329678189246025	47.29518041848225	15.698720764353988
24	17.758480620998746	18.1996633794988	44.60804701803213	19.433808981470325
25	13.654509198757692	25.67722525197949	43.80303424899811	16.86523130026471
26	14.214636383778467	19.609893377594133	46.12322798070457	20.052242257922824
27	17.391540952255085	20.04330199056996	43.41005119274828	19.155105864426677
28	13.71164916836078	19.227405417802014	47.939268375164716	19.121677038672487
29	16.451258051099458	20.1560271006713	43.53132786292627	19.86138698530298
30	15.711548104468967	22.074297508775068	43.55465029949895	18.65950408725701
31	20.26719738166779	19.33974182062714	40.363363561802515	20.029697235902557
32	17.714945406063055	23.75312423473255	38.76150087653491	19.770429482669485
33	16.113471428071662	22.35960864951431	38.5617053365622	22.965214585851832
34	18.429000672463587	24.38438485130003	36.35656895861434	20.830045517622047
35	15.084174560663602	26.5389892833404	35.28140463261332	23.095431523382686
36	19.37317064638133	25.593264480317806	37.26225691218714	17.771307961113724
37	17.20418404512114	22.8898053742668	37.05701947034746	22.848991110264592
38	19.34479501521789	27.11738571034311	32.70194314767378	20.835876126765218
39	19.790642261032485	22.857931377617458	34.451514597901756	22.8999117634483
40	17.559851202854666	27.583445734520705	35.90527981093278	18.95142325169185
41	17.27920454942996	25.5322374379526	35.67749734707284	21.511060665544598
42	18.222985816071493	24.301201494190767	38.41671752253531	19.059095167202436
43	20.51052813657619	22.01093822275259	36.313033743678645	21.16549989699257
44	19.240621465193204	22.089068385271105	35.86679779058784	22.80351235894785
45	18.001811375907145	21.3691825097274	35.67788605434905	24.951120060016404
46	22.358442527685675	24.050096593758138	34.89153123457318	18.69992964398301
47	15.84642952931436	21.54526690585121	43.00307467455483	19.605228890279598
48	19.00584227036146	22.63675693745311	35.831814135728806	22.52558665645662
49	17.5812301030463	19.857888619817075	42.056183749703614	20.504697527433017
50	17.94894718634238	19.83689842690165	39.31229908692661	22.901855299829357
51	17.71377928423442	20.193731706463815	35.39762810820056	26.694860901101208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	170.0
1	436.5
2	703.0
3	10728.0
4	20753.0
5	13552.0
6	6351.0
7	6057.0
8	5763.0
9	5703.5
10	5644.0
11	5545.0
12	5446.0
13	5181.5
14	4917.0
15	4681.5
16	4446.0
17	4118.5
18	3791.0
19	3480.0
20	3169.0
21	2959.0
22	2749.0
23	2602.5
24	2456.0
25	2427.0
26	2375.5
27	2353.0
28	2586.0
29	2819.0
30	3016.5
31	3214.0
32	3525.0
33	3836.0
34	4325.5
35	4815.0
36	4844.5
37	4874.0
38	5261.0
39	5648.0
40	6412.0
41	7176.0
42	8504.0
43	9832.0
44	10709.0
45	11586.0
46	26171.5
47	40757.0
48	29188.0
49	17619.0
50	16986.5
51	16354.0
52	14234.0
53	12114.0
54	11092.0
55	10070.0
56	9133.5
57	8197.0
58	7443.5
59	6690.0
60	6061.5
61	5433.0
62	4874.0
63	4315.0
64	4061.5
65	3808.0
66	3222.0
67	2636.0
68	2171.5
69	1707.0
70	1432.5
71	1158.0
72	919.0
73	680.0
74	530.0
75	294.0
76	208.0
77	154.5
78	101.0
79	79.0
80	57.0
81	45.5
82	34.0
83	23.5
84	13.0
85	11.5
86	10.0
87	6.5
88	3.0
89	3.0
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	257263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.39025421056179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.39267476910356	32.3830490328707
2	9.076595049360781	7.695183430174605
3	3.8896437720944554	4.946489648628374
4	2.565764470202892	4.3505363254531195
5	1.8469938112668296	3.9147268592467652
6	1.419223492127329	3.6096746767727237
7	1.0586316205347108	3.1412964456863772
8	0.8405220319631485	2.850395408359665
9	0.640348835513799	2.4430094928780446
>10	2.1899320441741654	13.572267263584475
>50	0.04581030960355061	1.3023466120847114
>100	0.022905154801775306	1.9769494925097904
>500	0.0	0.0
>1k	0.0069711340701055285	4.192416273780638
>5k	0.0029876288871880834	8.083836393779622
>10k+	9.95876295729361E-4	5.537822644190355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13118	5.099062049342502	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC	7412	2.881098331279663	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	6689	2.6000629705787466	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	5048	1.9621943303156693	No Hit
GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	1700	0.6608023695595557	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	1660	0.6452540785110957	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	1560	0.6063833508899453	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTC	1485	0.5772303051740826	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTA	1321	0.513482311875396	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	1170	0.45478751316745897	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	1035	0.402312030878906	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	400	0.15548291048460136	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	367	0.14265557036962176	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCACATT	364	0.14148944854098724	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACGCACATTCGT	335	0.13021693753085364	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	332	0.12905081570221913	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACGCACATTCGTAT	307	0.11933313379693154	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACGCACATTCGTATG	287	0.11155898827270147	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	272	0.10572837912952893	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1578151541418704	0.0
2	0.0	0.0	0.0	1.1093705663076308	0.0
3	0.0	0.0	0.0	1.3286014700909186	0.0
4	0.0	0.0	0.0	2.313197000734657	0.0
5	0.0	0.0	0.0	6.134966940446159	0.0
6	0.0	0.0	0.0	7.009169604645829	0.0
7	0.0	0.0	0.0	8.089387125237597	0.0
8	0.0	0.0	0.0	9.371343722183136	0.0
9	0.0	0.0	0.0	9.694359468714895	0.0
10	0.0	0.0	0.0	13.056677407944399	0.0
11	0.0	0.0	0.0	14.311035788278922	0.0
12	0.0	0.0	0.0	17.31535432611763	0.0
13	0.0	0.0	0.0	17.78879978854324	0.0
14	0.0	0.0	0.0	18.076831880215966	0.0
15	0.0	0.0	0.0	18.874070503725758	0.0
16	0.0	0.0	0.0	19.426812250498518	0.0
17	0.0	0.0	0.0	19.95273319521268	0.0
18	0.0	0.0	0.0	20.517136160271786	0.0
19	0.0	0.0	0.0	21.026731399385064	0.0
20	0.0	0.0	0.0	21.402222628205376	0.0
21	0.0	0.0	0.0	21.75711237138648	0.0
22	0.0	0.0	0.0	22.193241935295788	0.0
23	0.0	0.0	0.0	22.52286570552314	0.0
24	0.0	0.0	0.0	22.826446088244325	0.0
25	0.0	0.0	0.0	23.094654108830262	0.0
26	0.0	0.0	0.0	23.366360494902104	0.0
27	0.0	0.0	0.0	23.6466184410506	0.0
28	0.0	0.0	0.0	23.90549748700746	0.0
29	0.0	0.0	0.0	24.183811896774895	0.0
30	0.0	0.0	0.0	24.478063304867003	0.0
31	0.0	0.0	0.0	24.753268056424748	0.0
32	0.0	0.0	0.0	25.031971173468396	0.0
33	0.0	0.0	0.0	25.303677559540237	0.0
34	0.0	0.0	0.0	25.584712920241152	0.0
35	0.0	0.0	0.0	25.83504040612136	0.0
36	0.0	0.0	0.0	26.118019303203337	0.0
37	0.0	0.0	0.0	26.375732227331564	0.0
38	0.0	0.0	0.0	26.64899344250825	0.0
39	0.0	0.0	0.0	26.94674321608626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATA	30	2.1589112E-6	45.000004	6
ACTAAGG	30	2.1589112E-6	45.000004	2
CAATAGG	30	2.1589112E-6	45.000004	2
GCGAGAC	20	7.02307E-4	45.0	21
TCGCAGC	20	7.02307E-4	45.0	38
GATCGCA	20	7.02307E-4	45.0	36
CGCGGGT	20	7.02307E-4	45.0	4
GGGTCGT	20	7.02307E-4	45.0	7
AGGGTAC	20	7.02307E-4	45.0	6
CCATCGG	20	7.02307E-4	45.0	27
GTAGACG	20	7.02307E-4	45.0	22
CGGGACT	20	7.02307E-4	45.0	6
TAGGGCA	40	6.7793735E-9	45.0	5
CATCGGT	20	7.02307E-4	45.0	28
TGGATCG	20	7.02307E-4	45.0	34
CGATGAT	20	7.02307E-4	45.0	10
GCAATAG	20	7.02307E-4	45.0	1
ATCGGTG	20	7.02307E-4	45.0	29
AAGATCG	25	3.8823397E-5	45.0	1
CTAGGGC	35	1.2070086E-7	45.0	4
>>END_MODULE