FastQCFastQC Report
Sat 18 Jun 2016
SRR3552131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335115
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131253.916565954970682No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC80292.395893946854065No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG80042.38843382122555No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC63641.899049579994927No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC27820.8301627799412141No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT17880.5335481849514345No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT17390.518926338719544No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA16860.503110872387091No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT12220.36465094072184173No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC11980.3574892201184668No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT8740.2608059919729048No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC5960.17784939498381153No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATG4820.14383122211778046No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT4260.12712054070990556No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGT4210.12562851558420243No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC4150.1238380854333587No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCATTCTCG3710.11070826432717128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC302.160883E-645.0000046
GGGTGCG207.026127E-445.07
AGCCGGA207.026127E-445.021
ACAGTCC207.026127E-445.039
TACGGGT207.026127E-445.04
TCGATAG207.026127E-445.01
AGCGGGT351.208482E-745.04
CTACGAA253.884871E-545.011
TACAAGG207.026127E-445.02
CGTTTTT66850.043.956621
CGATGAA4400.042.95454419
CCGATGA4900.041.78571318
ACGGGCG603.6379788E-1241.2500045
GGCGAAC603.6379788E-1241.25000432
TACGGCT7100.041.1971867
GCGAACA501.0768417E-940.533
CGACAGG501.0768417E-940.52
CACAACG1500.040.512
ACGGCTG7200.040.31258
CGGCGAT451.9212166E-840.031