##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335115
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.655219849902274	28.0	16.0	31.0	16.0	33.0
2	26.63556391089626	30.0	25.0	31.0	16.0	33.0
3	26.856046431821913	30.0	25.0	33.0	16.0	33.0
4	29.59344702564791	32.0	28.0	35.0	19.0	37.0
5	32.143311997374035	33.0	32.0	35.0	28.0	37.0
6	30.885758620175164	35.0	30.0	35.0	17.0	37.0
7	33.07805977052654	35.0	32.0	35.0	28.0	37.0
8	33.24087253629351	35.0	33.0	37.0	28.0	37.0
9	35.12249526282022	37.0	34.0	39.0	30.0	39.0
10	35.17906987153663	37.0	34.0	39.0	30.0	39.0
11	35.369586559837664	37.0	34.0	39.0	30.0	39.0
12	35.25086015248497	37.0	34.0	39.0	28.0	39.0
13	35.41464273458365	37.0	34.0	39.0	30.0	39.0
14	35.7532071080077	38.0	34.0	40.0	27.0	41.0
15	36.33898810855975	38.0	34.0	40.0	30.0	41.0
16	36.091070229622666	38.0	34.0	40.0	29.0	41.0
17	35.630804350745265	37.0	34.0	40.0	27.0	41.0
18	35.468483953269775	38.0	34.0	39.0	27.0	40.0
19	34.758420243796905	37.0	33.0	39.0	27.0	40.0
20	34.78381749548662	35.0	34.0	39.0	29.0	40.0
21	35.29642958387419	36.0	34.0	39.0	30.0	40.0
22	35.86342897214389	37.0	34.0	39.0	31.0	40.0
23	36.27773450904913	37.0	35.0	40.0	31.0	41.0
24	36.219035256553724	37.0	35.0	40.0	31.0	41.0
25	34.609701147367325	35.0	33.0	39.0	26.0	40.0
26	35.060961162585976	36.0	33.0	39.0	28.0	40.0
27	35.599230115035134	37.0	35.0	39.0	30.0	40.0
28	35.51789385733256	37.0	35.0	40.0	30.0	41.0
29	35.63005237008191	37.0	35.0	40.0	30.0	41.0
30	34.703653969532844	36.0	34.0	40.0	25.0	41.0
31	34.742446622801125	36.0	34.0	39.0	27.0	41.0
32	34.29872730256778	37.0	33.0	40.0	23.0	40.0
33	34.001915760261404	37.0	33.0	40.0	20.0	41.0
34	33.73374214821778	38.0	33.0	40.0	15.0	41.0
35	33.352410366590576	37.0	33.0	40.0	14.0	41.0
36	33.113543112066004	37.0	33.0	40.0	11.0	41.0
37	33.10742282500037	38.0	33.0	40.0	10.0	41.0
38	32.919588797875356	37.0	33.0	40.0	10.0	41.0
39	32.76177730032974	37.0	33.0	40.0	10.0	41.0
40	32.532470942810676	36.0	31.0	40.0	10.0	41.0
41	32.208588096623544	36.0	31.0	40.0	10.0	41.0
42	32.519663399131645	37.0	32.0	40.0	10.0	41.0
43	32.54835205824866	37.0	32.0	40.0	10.0	41.0
44	32.67070408665682	37.0	33.0	40.0	10.0	41.0
45	32.705918863673666	37.0	33.0	40.0	10.0	41.0
46	32.51718066932247	37.0	32.0	40.0	10.0	41.0
47	32.37319726064187	36.0	31.0	40.0	10.0	41.0
48	32.43022246094624	36.0	32.0	40.0	10.0	41.0
49	32.49457947271832	36.0	32.0	40.0	10.0	41.0
50	32.39959416916581	36.0	32.0	40.0	10.0	41.0
51	31.381457111737763	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	14.0
10	14.0
11	12.0
12	10.0
13	14.0
14	25.0
15	35.0
16	82.0
17	148.0
18	360.0
19	662.0
20	1258.0
21	2038.0
22	2857.0
23	4038.0
24	5508.0
25	8715.0
26	11160.0
27	11615.0
28	11075.0
29	10767.0
30	11889.0
31	14789.0
32	18891.0
33	23958.0
34	28559.0
35	43048.0
36	49469.0
37	47924.0
38	23965.0
39	2212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53857630962505	12.088089163421511	16.13595332945407	30.237381197499364
2	40.6191904271668	26.190412246542234	18.051116780806588	15.139280545484384
3	22.4505020664548	25.862763528937826	38.065141816988195	13.621592587619174
4	20.429404831177354	17.337630365695357	47.902958685824274	14.330006117303014
5	26.16474941438014	20.5472748161079	37.418498127508464	15.86947764200349
6	18.34922340092207	30.64112319651463	38.269549259209526	12.740104143353776
7	56.78946033451203	4.091729704728228	35.59822747415066	3.5205824866090745
8	55.18284767915491	11.301791922175969	27.972188651656893	5.543171747012219
9	50.88820255733106	5.042149709801113	28.264327171269564	15.805320561598258
10	31.99588201065306	18.929621174820586	34.796711576622954	14.277785237903407
11	26.680990107873413	17.83089387225281	39.681601838174956	15.80651418169882
12	20.730793906569385	16.22756367217224	43.45373976097757	19.587902660280797
13	20.035211792966592	18.011428912462886	47.9545827551736	13.998776539396923
14	16.729480924458766	23.880160541903525	41.44517553675604	17.945182996881666
15	14.506960297211405	18.623457619026304	50.101308506035245	16.76827357772705
16	16.882264297330767	20.035510197991734	41.3860913417782	21.696134162899302
17	16.95984960386733	20.46461662414395	43.74796711576623	18.82756665622249
18	18.363546842128823	18.519612670277365	43.48208823836593	19.634752249227876
19	16.753651731495157	21.666293660385243	40.102054518598095	21.478000089521508
20	19.816182504513378	20.77585306536562	44.92845739522254	14.479507034898468
21	18.599286811989916	24.913537143965506	41.15661787744506	15.330558166599525
22	16.30425376363338	17.9806931948734	44.17349268161676	21.54156035987646
23	17.528311176760216	23.276188771018905	42.53435387851931	16.661146173701567
24	19.574474434149472	19.442281008012174	40.59710845530638	20.386136102531964
25	15.32906614147382	25.33190098921266	39.45242677886696	19.886606090446563
26	15.576742312340539	18.394282559718306	43.95535860823897	22.073616519702192
27	18.849648628082896	21.186756784984258	39.62013040299598	20.343464183936856
28	13.645763394655566	21.77192903928502	45.18538412186861	19.3969234441908
29	17.878340271250167	18.74222281903227	41.77371946943587	21.605717440281694
30	16.01778493949838	24.40415976605046	42.1610491920684	17.417006102382764
31	18.829357086373335	18.921564239141787	41.71015919908091	20.538919475403965
32	20.076391686435997	21.954254509645942	39.83140116079555	18.13795264312251
33	18.14720319890187	20.044462348745952	36.534324038016806	25.27401041433538
34	17.823732151649434	20.319293376900465	40.17068767438043	21.686286797069663
35	17.279142980767794	24.485027527863572	34.43146382585083	23.804365665517807
36	19.94867433567581	24.470107276606537	34.88802351431598	20.69319487340167
37	17.94577980693195	24.884591856526864	38.45306834967101	18.71655998687018
38	18.63479700998165	23.79093743938648	33.70126672933172	23.87299882130015
39	20.97518762215956	21.31656297092043	37.54502185816809	20.163227548751923
40	19.4240783014786	20.757053548781762	34.857287796726496	24.961580353013144
41	20.079972546737686	23.631589155961386	34.269131492174324	22.019306805126597
42	19.089267863270816	21.36371096489265	36.601763573698584	22.945257598137953
43	23.38898587052206	20.84388941109768	32.02124643779001	23.745878280590247
44	20.003282455276548	21.81430255285499	34.715545409784696	23.466869582083763
45	18.532444086358417	20.288259254285844	35.086462856034494	26.09283380332125
46	22.624770601136923	23.346313951926952	33.56967011324471	20.45924533369142
47	16.614296584754488	21.749250257374335	40.83046118496635	20.805991972904824
48	19.591185115557348	21.99125673276338	35.01424883995046	23.40330931172881
49	19.187443116542084	19.195201647195738	39.4637661698223	22.15358906643988
50	19.403786759769034	19.386180863285738	36.733658594810734	24.47637378213449
51	18.173462841114244	19.877952344717485	34.30464169016606	27.643943124002206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	387.5
2	686.0
3	11459.0
4	22232.0
5	14320.0
6	6408.0
7	5952.0
8	5496.0
9	5361.0
10	5226.0
11	5020.0
12	4814.0
13	4628.5
14	4443.0
15	4179.5
16	3916.0
17	3601.5
18	3287.0
19	3093.0
20	2899.0
21	2757.5
22	2616.0
23	2540.0
24	2464.0
25	2500.5
26	2687.5
27	2838.0
28	3199.0
29	3560.0
30	3792.5
31	4025.0
32	4533.0
33	5041.0
34	5483.5
35	5926.0
36	6588.0
37	7250.0
38	7798.0
39	8346.0
40	8827.5
41	9309.0
42	10930.0
43	12551.0
44	14127.5
45	15704.0
46	32454.5
47	49205.0
48	37944.0
49	26683.0
50	26364.5
51	26046.0
52	22561.0
53	19076.0
54	17532.0
55	15988.0
56	14817.0
57	13646.0
58	12466.0
59	11286.0
60	10449.5
61	9613.0
62	8434.0
63	7255.0
64	6424.0
65	5593.0
66	4717.0
67	3841.0
68	2984.5
69	2128.0
70	1765.0
71	1402.0
72	1135.5
73	869.0
74	665.5
75	335.5
76	209.0
77	146.5
78	84.0
79	55.5
80	27.0
81	21.5
82	16.0
83	12.0
84	8.0
85	7.5
86	7.0
87	5.5
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335115.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.190043156776085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49159957370253	34.685471726063014
2	8.17337134672501	7.223632650962706
3	3.354540590613154	4.447118804110571
4	2.087194983345746	3.6893294556262015
5	1.6206911398247301	3.580920570632972
6	1.25554127960037	3.3289453998392524
7	0.9456449927029589	2.9251665126973068
8	0.810769699542605	2.8662358410395274
9	0.644464250831804	2.563101275153111
>10	2.5263698111348574	16.749549492744034
>50	0.055792509257945586	1.5845176555474696
>100	0.02585506526587722	2.102267067853493
>500	0.00136079290873038	0.4419812056270931
>1k	0.00408237872619114	3.1314518752422957
>5k	0.00204118936309557	6.734049702333336
>10k+	6.8039645436519E-4	3.946260764527617
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13125	3.916565954970682	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	8029	2.395893946854065	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	8004	2.38843382122555	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	6364	1.899049579994927	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2782	0.8301627799412141	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	1788	0.5335481849514345	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT	1739	0.518926338719544	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA	1686	0.503110872387091	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	1222	0.36465094072184173	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	1198	0.3574892201184668	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	874	0.2608059919729048	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	596	0.17784939498381153	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATG	482	0.14383122211778046	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	426	0.12712054070990556	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGT	421	0.12562851558420243	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	415	0.1238380854333587	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCATTCTCG	371	0.11070826432717128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13338704623785866	0.0
2	0.0	0.0	0.0	1.2327111588559152	0.0
3	0.0	0.0	0.0	1.4359249809766796	0.0
4	0.0	0.0	0.0	2.2550467749876906	0.0
5	0.0	0.0	0.0	5.45723109977172	0.0
6	0.0	0.0	0.0	6.15132118824881	0.0
7	0.0	0.0	0.0	6.98416961341629	0.0
8	2.984050251406234E-4	0.0	0.0	7.996061053668144	0.0
9	2.984050251406234E-4	0.0	0.0	8.275964967250049	0.0
10	2.984050251406234E-4	0.0	0.0	11.453381674947407	0.0
11	2.984050251406234E-4	0.0	0.0	12.430658132282948	0.0
12	2.984050251406234E-4	0.0	0.0	15.220148307297494	0.0
13	2.984050251406234E-4	0.0	0.0	15.726541634961134	0.0
14	2.984050251406234E-4	0.0	0.0	15.949151783716038	0.0
15	2.984050251406234E-4	0.0	0.0	16.750966086268892	0.0
16	2.984050251406234E-4	0.0	0.0	17.32330692448861	0.0
17	2.984050251406234E-4	0.0	0.0	17.938916491353716	0.0
18	2.984050251406234E-4	0.0	0.0	18.63986989540904	0.0
19	2.984050251406234E-4	0.0	0.0	19.595661190934454	0.0
20	2.984050251406234E-4	0.0	0.0	20.108321024126045	0.0
21	2.984050251406234E-4	0.0	0.0	20.56965519299345	0.0
22	2.984050251406234E-4	0.0	0.0	21.080823001059336	0.0
23	2.984050251406234E-4	0.0	0.0	21.53678587947421	0.0
24	2.984050251406234E-4	0.0	0.0	21.90173522522119	0.0
25	2.984050251406234E-4	0.0	0.0	22.22460946242335	0.0
26	2.984050251406234E-4	0.0	0.0	22.604479059427362	0.0
27	2.984050251406234E-4	0.0	0.0	22.980767796129687	0.0
28	2.984050251406234E-4	0.0	0.0	23.31169896901064	0.0
29	2.984050251406234E-4	0.0	0.0	23.6375572564642	0.0
30	2.984050251406234E-4	0.0	0.0	24.00161138713576	0.0
31	2.984050251406234E-4	0.0	0.0	24.36059263237993	0.0
32	2.984050251406234E-4	0.0	0.0	24.718678662548676	0.0
33	2.984050251406234E-4	0.0	0.0	25.101830714829237	0.0
34	2.984050251406234E-4	0.0	0.0	25.459021529922563	0.0
35	2.984050251406234E-4	0.0	0.0	25.808453814362235	0.0
36	2.984050251406234E-4	0.0	0.0	26.15579726362592	0.0
37	2.984050251406234E-4	0.0	0.0	26.485236411381166	0.0
38	2.984050251406234E-4	0.0	0.0	26.868090058636586	0.0
39	2.984050251406234E-4	0.0	0.0	27.254524566193695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	30	2.160883E-6	45.000004	6
GGGTGCG	20	7.026127E-4	45.0	7
AGCCGGA	20	7.026127E-4	45.0	21
ACAGTCC	20	7.026127E-4	45.0	39
TACGGGT	20	7.026127E-4	45.0	4
TCGATAG	20	7.026127E-4	45.0	1
AGCGGGT	35	1.208482E-7	45.0	4
CTACGAA	25	3.884871E-5	45.0	11
TACAAGG	20	7.026127E-4	45.0	2
CGTTTTT	6685	0.0	43.95662	1
CGATGAA	440	0.0	42.954544	19
CCGATGA	490	0.0	41.785713	18
ACGGGCG	60	3.6379788E-12	41.250004	5
GGCGAAC	60	3.6379788E-12	41.250004	32
TACGGCT	710	0.0	41.197186	7
GCGAACA	50	1.0768417E-9	40.5	33
CGACAGG	50	1.0768417E-9	40.5	2
CACAACG	150	0.0	40.5	12
ACGGCTG	720	0.0	40.3125	8
CGGCGAT	45	1.9212166E-8	40.0	31
>>END_MODULE