##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	362453
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.035905344968864	30.0	25.0	33.0	16.0	33.0
2	28.744394445624675	31.0	28.0	33.0	16.0	33.0
3	28.481477598474836	31.0	28.0	33.0	16.0	34.0
4	32.352536742694916	35.0	32.0	35.0	28.0	37.0
5	33.66952128965686	35.0	33.0	35.0	30.0	37.0
6	33.4937522934008	35.0	33.0	35.0	28.0	37.0
7	34.267651254093636	35.0	33.0	36.0	32.0	37.0
8	34.236521700744646	35.0	35.0	37.0	31.0	37.0
9	36.02839816472756	39.0	35.0	39.0	30.0	39.0
10	36.02611373060783	37.0	35.0	39.0	31.0	39.0
11	36.20614258952195	37.0	35.0	39.0	32.0	39.0
12	36.15521460713527	37.0	35.0	39.0	32.0	39.0
13	36.167737610117726	37.0	35.0	39.0	32.0	39.0
14	37.34853070605016	39.0	36.0	40.0	32.0	41.0
15	37.25448816812111	39.0	36.0	41.0	32.0	41.0
16	37.19557018427217	39.0	36.0	41.0	32.0	41.0
17	36.25805552719939	38.0	34.0	40.0	29.0	41.0
18	35.84713328348779	38.0	34.0	39.0	29.0	40.0
19	35.3077392103252	37.0	34.0	39.0	29.0	40.0
20	35.38985192562898	36.0	34.0	39.0	30.0	40.0
21	35.968125522481536	37.0	35.0	39.0	31.0	40.0
22	36.373052506118036	38.0	35.0	40.0	32.0	41.0
23	36.60581096031761	38.0	35.0	40.0	32.0	41.0
24	36.50615941929023	38.0	35.0	40.0	32.0	41.0
25	35.86620334222644	37.0	35.0	40.0	31.0	41.0
26	36.00361426171117	37.0	35.0	40.0	31.0	41.0
27	36.36842569933205	38.0	35.0	40.0	32.0	41.0
28	36.16705062449476	38.0	35.0	40.0	31.0	41.0
29	36.164937247036164	38.0	35.0	40.0	31.0	41.0
30	35.79390983106775	38.0	35.0	40.0	30.0	41.0
31	35.60051923973591	38.0	35.0	40.0	30.0	41.0
32	35.26976739053063	38.0	35.0	40.0	26.0	41.0
33	34.66502967281275	38.0	34.0	40.0	21.0	41.0
34	34.23833986751386	38.0	34.0	40.0	18.0	41.0
35	33.81086651234781	38.0	34.0	40.0	15.0	41.0
36	33.62113708536003	38.0	34.0	40.0	13.0	41.0
37	33.378473898684796	38.0	33.0	40.0	10.0	41.0
38	33.285391485240844	38.0	33.0	40.0	10.0	41.0
39	33.1359348660378	38.0	33.0	40.0	10.0	41.0
40	32.85194770080535	37.0	32.0	40.0	10.0	41.0
41	32.540147274267284	37.0	31.0	40.0	10.0	41.0
42	32.63760818644072	37.0	32.0	40.0	10.0	41.0
43	32.63055623763633	37.0	32.0	40.0	10.0	41.0
44	32.53648335094481	37.0	32.0	40.0	10.0	41.0
45	32.530816409299966	37.0	32.0	40.0	10.0	41.0
46	32.30767851280028	36.0	31.0	40.0	10.0	41.0
47	32.23620441822802	36.0	31.0	40.0	10.0	41.0
48	32.22401525163262	36.0	31.0	40.0	10.0	41.0
49	32.22548302814434	36.0	31.0	40.0	10.0	41.0
50	32.151561719726416	36.0	31.0	40.0	10.0	41.0
51	30.212965543118695	35.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	9.0
10	11.0
11	9.0
12	8.0
13	21.0
14	22.0
15	33.0
16	81.0
17	189.0
18	417.0
19	674.0
20	966.0
21	1504.0
22	2185.0
23	3319.0
24	5176.0
25	8243.0
26	11832.0
27	12881.0
28	12314.0
29	11430.0
30	11126.0
31	12313.0
32	15007.0
33	20616.0
34	27549.0
35	34781.0
36	41598.0
37	57089.0
38	55991.0
39	15053.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28051637039837	12.178682477452249	15.827431418694285	29.713369733455096
2	39.863375389360826	28.78000733888256	17.769752216149403	13.586865055607209
3	21.87180130941115	28.626056343856998	37.55604174886123	11.94610059787062
4	20.303046188057486	17.028690616438542	48.69569295881121	13.972570236692757
5	25.617390392685397	20.544456798536636	37.172267852659516	16.665884956118447
6	17.998747423803916	31.812124606500703	38.81523949312049	11.373888476574894
7	56.96490303570394	4.387879256069063	34.95542870385954	3.691789004367463
8	54.493686077919065	11.30077554882978	28.893125453507075	5.3124129197440775
9	50.380049275354324	5.508576284373422	28.51100694434865	15.600367495923608
10	30.321448574022007	19.052677174695752	35.57426756020781	15.05160669107443
11	25.167676912592807	17.73002292711055	40.385925899357986	16.71637426093866
12	21.320281526156496	16.16844114961112	44.41431026919352	18.09696705503886
13	18.204567212852425	18.09945013560379	49.83680642731609	13.859176224227692
14	16.83859700430125	23.416829216477723	41.27073027399415	18.473843505226885
15	14.313855865450142	18.666971993610208	52.14441596565623	14.874756175283416
16	16.67057521940776	21.048522153217107	40.89495741516831	21.385945212206824
17	16.782589742670083	19.014879170540734	44.59585104827384	19.60668003851534
18	18.33975715472075	19.484181397312202	43.70470102330509	18.471360424661956
19	16.75086149100711	22.581686453140133	39.9582842465092	20.70916780934356
20	18.42500958744996	21.617975296107357	45.2453145649229	14.711700551519783
21	18.159871762683714	25.186713863590587	41.602083580491815	15.05133079323388
22	15.955448016708374	18.153802010191665	42.58041732307361	23.310332650026346
23	16.30363109147945	24.397921937464993	42.92280654319319	16.375640427862372
24	20.34746574038565	18.19408309491162	39.98311505215849	21.475336112544248
25	15.17465712795866	26.992189332134096	38.824895917539656	19.008257622367587
26	15.626577790775631	18.346102805053345	42.67974054567075	23.347578858500277
27	18.70697718048961	21.644737386640475	38.585140694103785	21.06314473876613
28	13.58438197504228	20.129782344193593	45.865808808314455	20.42002687244967
29	18.527919481974216	18.72739362069013	39.72680595828976	23.01788093904589
30	15.911304362220758	24.78942097320204	42.01372315858884	17.285551505988362
31	19.049090502768635	18.60682626437083	42.17098492770097	20.173098305159563
32	19.701037099982617	21.943810645794077	40.43034545168615	17.92480680253716
33	17.099044565778186	19.762286420584186	36.50376738501267	26.634901628624952
34	17.9656396829382	19.87209376112213	40.65271911116752	21.50954744477215
35	16.290112097292614	24.851222089484708	33.88246200196991	24.97620381125277
36	20.701166771967674	23.323299848532088	35.40596987747377	20.56956350202647
37	17.714296750199335	24.777557366058495	39.2448124308531	18.263333452889064
38	18.37672746535413	23.248807431584233	33.63111906923105	24.743346033830594
39	21.659635870030044	20.42582072710117	38.3944952862854	19.520048116583393
40	19.3236088541135	20.493139800194786	35.17062901948667	25.012622326205054
41	20.29366566147887	24.216105260544126	34.08910948454007	21.401119593436942
42	19.020397127351686	21.01127594474318	37.651226503850154	22.31710042405498
43	24.587739651761744	20.692889836751245	31.286263322416975	23.433107189070032
44	20.46499822045893	20.955544580952566	34.66711546048729	23.91234173810122
45	18.33037662814213	20.005904213787716	34.65718313822757	27.006536019842574
46	23.555881728113715	23.585126899211758	32.641473515186796	20.217517857487728
47	16.601600759270855	20.595497899037944	42.29679434299068	20.50610699870052
48	20.17392599868121	21.84917768648625	34.01516886327331	23.961727451559238
49	18.671110461218422	18.802989629000173	40.49931991182305	22.026579997958358
50	19.22704460992184	18.931558022695356	37.25889977459146	24.58249759279134
51	18.636347333309423	19.19559225609941	33.761066952128964	28.4069934584622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	423.5
2	721.0
3	12211.0
4	23701.0
5	15251.5
6	6802.0
7	6249.0
8	5696.0
9	5617.0
10	5538.0
11	5382.0
12	5226.0
13	5021.5
14	4817.0
15	4575.5
16	4334.0
17	4072.5
18	3811.0
19	3550.5
20	3290.0
21	3059.0
22	2828.0
23	2744.5
24	2661.0
25	2835.0
26	2987.5
27	2966.0
28	3335.0
29	3704.0
30	3998.0
31	4292.0
32	4746.5
33	5201.0
34	5842.0
35	6483.0
36	7278.5
37	8074.0
38	8599.5
39	9125.0
40	9804.0
41	10483.0
42	12164.0
43	13845.0
44	15733.5
45	17622.0
46	39588.5
47	61555.0
48	44321.0
49	27087.0
50	26684.5
51	26282.0
52	22823.0
53	19364.0
54	17713.0
55	16062.0
56	14809.5
57	13557.0
58	12417.0
59	11277.0
60	10665.0
61	10053.0
62	8733.5
63	7414.0
64	6656.5
65	5899.0
66	4983.5
67	4068.0
68	3115.0
69	2162.0
70	1851.0
71	1540.0
72	1188.5
73	837.0
74	655.0
75	353.0
76	233.0
77	167.0
78	101.0
79	78.5
80	56.0
81	46.0
82	36.0
83	27.5
84	19.0
85	14.0
86	9.0
87	7.0
88	5.0
89	5.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	362453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.487271373835895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41784231577543	32.533423047052466
2	7.781111036283585	6.45634130304493
3	3.20729873132196	3.9918621852994116
4	2.0196176032445847	3.3515369430873645
5	1.5650041595351585	3.246387613390854
6	1.2486676606362717	3.108228845552989
7	1.0202683860381592	2.962970598399746
8	0.862528641044747	2.8627167838983447
9	0.6720687666072325	2.509406937190209
>10	3.103873219291685	19.387423559331594
>50	0.06758991777855415	1.8210130050486817
>100	0.026099077162015958	1.881259966795223
>500	0.0013384142134367158	0.29512682182752686
>1k	0.004015242640310147	3.2003075025455336
>5k	6.692071067183579E-4	2.2202532400326738
>10k+	0.0020076213201550737	10.171741647502447
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14105	3.8915390409239268	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	11430	3.153512317459091	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	11102	3.0630178257594776	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	7997	2.2063550308591733	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2959	0.8163817101803543	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	2326	0.6417383771137223	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	1744	0.48116583391501794	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	1703	0.4698540224525663	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA	1563	0.431228324775902	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	1232	0.3399061395546457	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	548	0.1511920166200859	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT	515	0.14208738788201505	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	422	0.11642888871108804	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATG	386	0.1064965664513744	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGT	386	0.1064965664513744	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	386	0.1064965664513744	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTAT	385	0.10622066861082678	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7589784054760205E-4	0.0	0.0	0.13049967857901576	0.0
2	2.7589784054760205E-4	0.0	0.0	1.25754235721597	0.0
3	2.7589784054760205E-4	0.0	0.0	1.4611549635401004	0.0
4	2.7589784054760205E-4	0.0	0.0	2.40693276093728	0.0
5	2.7589784054760205E-4	0.0	0.0	6.58347427114688	0.0
6	2.7589784054760205E-4	0.0	0.0	7.384681600097116	0.0
7	2.7589784054760205E-4	0.0	0.0	8.343702493840581	0.0
8	2.7589784054760205E-4	0.0	0.0	9.452535915001393	0.0
9	2.7589784054760205E-4	0.0	0.0	9.750505582792803	0.0
10	2.7589784054760205E-4	0.0	0.0	13.65942618767123	0.0
11	2.7589784054760205E-4	0.0	0.0	14.589201910316648	0.0
12	2.7589784054760205E-4	0.0	0.0	17.78327121033624	0.0
13	2.7589784054760205E-4	0.0	0.0	18.26719602265673	0.0
14	2.7589784054760205E-4	0.0	0.0	18.513572794265738	0.0
15	2.7589784054760205E-4	0.0	0.0	19.22594101855965	0.0
16	2.7589784054760205E-4	0.0	0.0	19.807257768593445	0.0
17	2.7589784054760205E-4	0.0	0.0	20.417267894044194	0.0
18	2.7589784054760205E-4	0.0	0.0	21.089079135777606	0.0
19	2.7589784054760205E-4	0.0	0.0	21.629563005410358	0.0
20	2.7589784054760205E-4	0.0	0.0	22.045892846796686	0.0
21	2.7589784054760205E-4	0.0	0.0	22.48622580031066	0.0
22	2.7589784054760205E-4	0.0	0.0	22.983669606817987	0.0
23	2.7589784054760205E-4	0.0	0.0	23.43227949554839	0.0
24	2.7589784054760205E-4	0.0	0.0	23.793153870984654	0.0
25	2.7589784054760205E-4	0.0	0.0	24.11678203794699	0.0
26	2.7589784054760205E-4	0.0	0.0	24.439306613547135	0.0
27	2.7589784054760205E-4	0.0	0.0	24.77590197901521	0.0
28	2.7589784054760205E-4	0.0	0.0	25.095115780528786	0.0
29	2.7589784054760205E-4	0.0	0.0	25.427572678388646	0.0
30	2.7589784054760205E-4	0.0	0.0	25.81686453140131	0.0
31	2.7589784054760205E-4	0.0	0.0	26.167530686737315	0.0
32	2.7589784054760205E-4	0.0	0.0	26.501367073799912	0.0
33	2.7589784054760205E-4	0.0	0.0	26.85479220754139	0.0
34	2.7589784054760205E-4	0.0	0.0	27.198009121182608	0.0
35	2.7589784054760205E-4	0.0	0.0	27.54536450243204	0.0
36	2.7589784054760205E-4	0.0	0.0	27.876993706770257	0.0
37	2.7589784054760205E-4	0.0	0.0	28.206415728384094	0.0
38	2.7589784054760205E-4	0.0	0.0	28.559564964285023	0.0
39	2.7589784054760205E-4	0.0	0.0	28.928164479256623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	30	2.1613741E-6	45.000004	2
GCTACGA	30	2.1613741E-6	45.000004	4
AGGGCCG	35	1.2088458E-7	45.000004	6
ACCGACG	20	7.026889E-4	45.0	17
CTAAGTT	20	7.026889E-4	45.0	12
CTAACGG	25	3.885504E-5	45.0	2
CTACGCG	20	7.026889E-4	45.0	1
AACACGT	75	0.0	45.0	41
CGTTTTT	7165	0.0	44.057922	1
TACGGCT	870	0.0	43.965515	7
ACGGCTG	900	0.0	42.5	8
CGGCTGT	930	0.0	41.85484	9
GAATCTG	1360	0.0	40.863968	1
CGAGACA	95	0.0	40.263157	22
ACACGAC	90	0.0	40.0	26
GGCCGAT	45	1.9221261E-8	40.0	8
GACGGGT	45	1.9221261E-8	40.0	4
GGCGACT	260	0.0	39.807693	10
ACACGTG	85	0.0	39.705883	42
AAACACG	85	0.0	39.705883	40
>>END_MODULE