SRR3552129_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309496
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15999	5.1693721405123165	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	6534	2.1111742962752347	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	6195	2.0016413782407527	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	6187	1.99905653061752	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	2009	0.6491198593842893	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA	1737	0.5612350401943805	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	1531	0.4946752138961408	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT	1514	0.48918241269677154	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTC	1269	0.4100214542352728	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	1210	0.39095820301393236	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC	957	0.30921239692920105	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGT	501	0.16187608240494222	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	488	0.15767570501718922	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	447	0.14442836094812211	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATG	424	0.13699692403132835	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	367	0.11857988471579599	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	327	0.10565564659963296	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	324	0.10468632874092072	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	20	7.0252887E-4	45.0	34
AATGGGC	20	7.0252887E-4	45.0	4
TTAAGTC	20	7.0252887E-4	45.0	26
CGAATGC	20	7.0252887E-4	45.0	45
TTCGGGC	20	7.0252887E-4	45.0	4
CATCGAA	35	1.2080818E-7	45.0	42
ACGTAGG	25	3.8841787E-5	45.0	2
TCGAATG	25	3.8841787E-5	45.0	44
TGGTCAA	20	7.0252887E-4	45.0	14
GGCTACG	20	7.0252887E-4	45.0	1
CTAGCGG	20	7.0252887E-4	45.0	2
CGTTTTT	6785	0.0	44.30361	1
GATGAAT	540	0.0	42.916664	20
CCGATGA	510	0.0	42.794117	18
CGATGAA	520	0.0	42.40385	19
TACGGCT	700	0.0	41.464287	7
ACGGCTG	705	0.0	41.17021	8
ACCACCG	680	0.0	41.02941	14
AGCGACT	55	6.002665E-11	40.909092	19
GGCGACT	205	0.0	40.609756	10

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content