##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309496
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.95796391552718	28.0	16.0	31.0	16.0	33.0
2	26.76115038643472	30.0	25.0	33.0	16.0	33.0
3	27.05634967818647	30.0	25.0	33.0	16.0	33.0
4	29.4335112570114	32.0	28.0	35.0	19.0	37.0
5	32.124657507689925	33.0	32.0	35.0	28.0	37.0
6	30.948412903559337	35.0	30.0	35.0	17.0	37.0
7	33.047367332695735	35.0	32.0	35.0	28.0	37.0
8	33.20459715149792	35.0	33.0	37.0	28.0	37.0
9	35.11022113371417	37.0	34.0	39.0	30.0	39.0
10	35.20491702639129	37.0	34.0	39.0	30.0	39.0
11	35.39881290872903	37.0	34.0	39.0	30.0	39.0
12	35.26547677514411	37.0	34.0	39.0	28.0	39.0
13	35.42640938816657	37.0	34.0	39.0	30.0	39.0
14	35.8619271331455	38.0	34.0	40.0	27.0	41.0
15	36.36863158166826	38.0	34.0	40.0	30.0	41.0
16	36.09799803551581	38.0	34.0	40.0	28.0	41.0
17	35.60187853801018	37.0	33.0	40.0	27.0	41.0
18	35.45009628557396	38.0	34.0	39.0	27.0	40.0
19	34.75866247059736	37.0	33.0	39.0	27.0	40.0
20	34.72000284333239	35.0	34.0	39.0	29.0	40.0
21	35.2593571483961	36.0	34.0	39.0	30.0	40.0
22	35.805451443637395	37.0	34.0	39.0	30.0	40.0
23	36.211508387830534	37.0	35.0	40.0	31.0	41.0
24	36.130269858091864	37.0	35.0	40.0	31.0	41.0
25	34.643135937136506	35.0	33.0	39.0	27.0	40.0
26	34.980158063432164	36.0	33.0	39.0	27.0	40.0
27	35.48392547884302	37.0	35.0	39.0	30.0	40.0
28	35.45592511696435	37.0	35.0	40.0	30.0	41.0
29	35.52029428490191	37.0	35.0	40.0	30.0	41.0
30	34.56176493395714	36.0	33.0	40.0	25.0	41.0
31	34.53527670793807	36.0	34.0	39.0	25.0	40.0
32	34.05729314756895	36.0	33.0	39.0	23.0	40.0
33	33.726842996355366	37.0	33.0	40.0	18.0	41.0
34	33.43685863468349	37.0	33.0	40.0	15.0	41.0
35	32.98259105125753	37.0	33.0	40.0	12.0	41.0
36	32.79573564763357	37.0	33.0	40.0	10.0	41.0
37	32.797199317600224	37.0	33.0	40.0	10.0	41.0
38	32.50232313180138	36.0	32.0	40.0	10.0	41.0
39	32.417113629901515	37.0	32.0	40.0	10.0	41.0
40	32.05323493680048	36.0	31.0	40.0	10.0	41.0
41	31.877310207563266	35.0	30.0	40.0	10.0	41.0
42	32.07477964174012	36.0	31.0	40.0	10.0	41.0
43	32.15456096363119	36.0	31.0	40.0	10.0	41.0
44	32.31322537286427	36.0	32.0	40.0	10.0	41.0
45	32.372896580246596	36.0	32.0	40.0	10.0	41.0
46	32.188222141804744	36.0	31.0	40.0	10.0	41.0
47	32.06923191252876	35.0	31.0	40.0	10.0	41.0
48	32.08569739188875	35.0	31.0	40.0	10.0	41.0
49	32.15247369917543	36.0	31.0	40.0	10.0	41.0
50	32.10045687181741	35.0	31.0	40.0	10.0	41.0
51	31.072747305296353	35.0	29.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	15.0
10	15.0
11	17.0
12	19.0
13	18.0
14	28.0
15	38.0
16	71.0
17	201.0
18	427.0
19	782.0
20	1318.0
21	1989.0
22	2818.0
23	3824.0
24	5524.0
25	8077.0
26	10647.0
27	11582.0
28	10707.0
29	10278.0
30	11191.0
31	13793.0
32	17514.0
33	21772.0
34	26106.0
35	41134.0
36	44238.0
37	42434.0
38	20860.0
39	2055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.430558068601854	11.436981414945588	16.1856696047768	32.946790911675755
2	41.9704939643808	26.298239718768578	18.162108718691034	13.56915759815959
3	22.402874350557035	25.017770827409723	39.50261069609947	13.076744125933775
4	20.185721301729263	17.132693152738646	49.59224028743506	13.089345258097035
5	26.229095044847107	20.00090469666813	38.7843461627937	14.985654095691059
6	18.040297774446195	30.647892056763254	39.207614961098045	12.104195207692507
7	54.065319099439094	4.297955385530023	37.84830821723059	3.788417297800295
8	53.06078269186032	11.820185075089823	29.603936722930186	5.515095510119679
9	48.81452425879495	5.252410370408665	30.224623258458916	15.708442112337478
10	33.53613616977279	16.98309509654406	36.438920050663015	13.041848683020135
11	26.588388864476435	17.032853413291285	41.003437847338894	15.375319874893375
12	20.624176079820096	15.11715821852302	44.54370977330886	19.714955928348026
13	20.529182929666295	17.142709437278672	48.71145345981854	13.616654173236487
14	16.577597125649444	22.9948044562773	43.25290149145708	17.174696926616175
15	13.908741954661771	17.69263576912141	50.856553881148706	17.54206839506811
16	16.203763538139427	18.734329361284153	42.434474112751055	22.62743298782537
17	16.243828676299533	20.68330446920154	44.70009305451443	18.37277379998449
18	16.78115387597901	18.296843901052032	45.18765993744669	19.73434228552227
19	16.347222581228838	20.20155349342156	42.560808540336545	20.890415385013053
20	19.37634088970455	19.466810556517693	46.82257605914131	14.33427249463644
21	17.7947372502391	24.524711143278104	42.42962752345749	15.250924083025305
22	16.172745366660635	17.73722439062217	46.1081887972704	19.98184144544679
23	17.563070282006876	22.584459896089125	43.21412877710859	16.63834104479541
24	18.85193992814124	19.613177553183238	41.76176751880477	19.77311499987076
25	15.142683588802441	24.72019024478507	40.831545480393935	19.30558068601856
26	15.23541499728591	18.72851345413188	45.04840127174503	20.987670276837182
27	18.69684907074728	21.223537622457155	41.05642722361517	19.023186083180395
28	14.662548142786983	21.331131904774214	44.94533047276863	19.060989479670173
29	17.734639542998938	18.326246542766302	41.5659653113449	22.37314860288986
30	16.977602295344692	25.455579393594746	41.8360818879727	15.73073642308786
31	22.503683407863107	18.771163439915217	37.92165326853982	20.803499883681855
32	20.255512187556544	23.10821464574663	37.865755938687414	18.77051722800941
33	19.764068033189446	23.565732675058808	35.617261612427946	21.052937679323804
34	22.015470313025048	21.040336547160546	36.23019360508698	20.713999534727428
35	17.749502416832527	25.571251324734405	36.047315635743274	20.631930622689794
36	20.72983172641973	27.511502571923387	32.311241502313436	19.44742419934345
37	19.325290149145708	25.916651588388866	35.37816320727893	19.3798950551865
38	20.77603587768501	27.192273890454153	31.663414066740764	20.368276165120065
39	22.697223873652646	23.715976943159202	32.51318272287848	21.073616460309665
40	19.361478015870965	24.983521596401893	36.42599581254685	19.22900457518029
41	20.327564815054153	26.635239227647528	32.67990539457699	20.357290562721328
42	18.492646108511902	22.835836327448497	38.560433737431175	20.11108382660842
43	20.010597875255254	25.031341277431697	32.83241140434771	22.125649442965337
44	20.18636751363507	22.692377284359086	34.89641223149895	22.224842970506888
45	19.84452141546256	21.010933905446276	35.2728306666322	23.871714012458966
46	22.79415565952387	24.338602114405354	33.76618760824049	19.10105461783028
47	16.640602786465738	23.487217928503114	41.027670793806706	18.844508491224442
48	19.7685915165301	23.480755809445032	35.148757980717036	21.60189469330783
49	19.168906867940137	20.251634916121695	40.26998733424665	20.309470881691524
50	18.85387856385866	21.160855067593765	37.759454080181975	22.225812288365603
51	17.830925116964355	20.625145397678807	35.2728306666322	26.271098818724635
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	606.0
2	920.0
3	13463.5
4	26007.0
5	16408.0
6	6809.0
7	6420.5
8	6032.0
9	5730.0
10	5428.0
11	5102.5
12	4777.0
13	4579.0
14	4381.0
15	4007.0
16	3633.0
17	3351.0
18	3069.0
19	2822.0
20	2575.0
21	2577.5
22	2580.0
23	2484.0
24	2388.0
25	2416.5
26	2557.5
27	2670.0
28	2840.0
29	3010.0
30	3285.0
31	3560.0
32	3956.0
33	4352.0
34	5133.0
35	5914.0
36	6111.0
37	6308.0
38	6598.0
39	6888.0
40	7764.0
41	8640.0
42	9445.0
43	10250.0
44	11456.5
45	12663.0
46	26688.5
47	40714.0
48	31834.5
49	22955.0
50	22351.5
51	21748.0
52	18916.5
53	16085.0
54	15133.0
55	14181.0
56	13111.5
57	12042.0
58	11390.5
59	10739.0
60	10522.5
61	10306.0
62	9326.0
63	8346.0
64	7360.5
65	6375.0
66	5203.0
67	4031.0
68	3438.0
69	2845.0
70	2225.5
71	1606.0
72	1262.0
73	918.0
74	728.0
75	400.0
76	262.0
77	187.5
78	113.0
79	83.5
80	54.0
81	38.5
82	23.0
83	17.5
84	12.0
85	10.0
86	8.0
87	5.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	309496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.61796953353422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4321402877286	34.99482845807756
2	8.20499858310972	7.321807536077619
3	3.663428250400143	4.903641901939264
4	2.2310180403033364	3.9817397980407794
5	1.6102826624549524	3.5923771386896717
6	1.2094085519164501	3.2376812353802356
7	0.960248076962982	2.9991023609798404
8	0.7455055550776256	2.661035531482721
9	0.5623569880809031	2.258210426504742
>10	2.290173441603139	15.234015738203176
>50	0.05324264558417016	1.6183223379253728
>100	0.028444701065515568	2.344012829293477
>500	0.0014587026187443883	0.4744649042218367
>1k	0.004376107856233165	3.016659576225258
>5k	0.0021880539281165824	6.155677728573568
>10k+	7.293513093721941E-4	5.206422498384887
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15999	5.1693721405123165	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	6534	2.1111742962752347	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	6195	2.0016413782407527	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	6187	1.99905653061752	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	2009	0.6491198593842893	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA	1737	0.5612350401943805	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	1531	0.4946752138961408	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT	1514	0.48918241269677154	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTC	1269	0.4100214542352728	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	1210	0.39095820301393236	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC	957	0.30921239692920105	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGT	501	0.16187608240494222	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	488	0.15767570501718922	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	447	0.14442836094812211	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATG	424	0.13699692403132835	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	367	0.11857988471579599	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	327	0.10565564659963296	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	324	0.10468632874092072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18061622767337865	0.0
2	0.0	0.0	0.0	1.1887068007340966	0.0
3	0.0	0.0	0.0	1.389678703440432	0.0
4	0.0	0.0	0.0	2.2074598702406494	0.0
5	0.0	0.0	0.0	4.892793444826427	0.0
6	0.0	0.0	0.0	5.3722826789360765	0.0
7	0.0	0.0	0.0	6.200726342182128	0.0
8	0.0	0.0	0.0	7.205585855713806	0.0
9	0.0	0.0	0.0	7.491857729986817	0.0
10	0.0	0.0	0.0	10.290601494041926	0.0
11	0.0	0.0	0.0	11.309354563548478	0.0
12	0.0	0.0	0.0	14.275790317160803	0.0
13	0.0	0.0	0.0	14.735893194096208	0.0
14	0.0	0.0	0.0	14.977576446868458	0.0
15	0.0	0.0	0.0	15.829606844676507	0.0
16	0.0	0.0	0.0	16.371778633649548	0.0
17	0.0	0.0	0.0	16.85643756300566	0.0
18	0.0	0.0	0.0	17.38956238529739	0.0
19	0.0	0.0	0.0	18.270026106960994	0.0
20	0.0	0.0	0.0	18.682309302866596	0.0
21	0.0	0.0	0.0	19.05291183084757	0.0
22	0.0	0.0	0.0	19.512045389924264	0.0
23	0.0	0.0	0.0	19.872954739318118	0.0
24	0.0	0.0	0.0	20.173120169566005	0.0
25	0.0	0.0	0.0	20.4422674283351	0.0
26	0.0	0.0	0.0	20.715291958539044	0.0
27	0.0	0.0	0.0	21.00834905782304	0.0
28	3.231059529040763E-4	0.0	0.0	21.248739886783675	0.0
29	3.231059529040763E-4	0.0	0.0	21.53695039677411	0.0
30	3.231059529040763E-4	0.0	0.0	21.825160906764545	0.0
31	3.231059529040763E-4	0.0	0.0	22.116925582236927	0.0
32	3.231059529040763E-4	0.0	0.0	22.3841342052886	0.0
33	3.231059529040763E-4	0.0	0.0	22.658128053351255	0.0
34	3.231059529040763E-4	0.0	0.0	22.947954093106212	0.0
35	3.231059529040763E-4	0.0	0.0	23.217747563781113	0.0
36	3.231059529040763E-4	0.0	0.0	23.48204823325665	0.0
37	3.231059529040763E-4	0.0	0.0	23.740209889627007	0.0
38	3.231059529040763E-4	0.0	0.0	24.05588440561429	0.0
39	3.231059529040763E-4	0.0	0.0	24.372528239460284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	20	7.0252887E-4	45.0	34
AATGGGC	20	7.0252887E-4	45.0	4
TTAAGTC	20	7.0252887E-4	45.0	26
CGAATGC	20	7.0252887E-4	45.0	45
TTCGGGC	20	7.0252887E-4	45.0	4
CATCGAA	35	1.2080818E-7	45.0	42
ACGTAGG	25	3.8841787E-5	45.0	2
TCGAATG	25	3.8841787E-5	45.0	44
TGGTCAA	20	7.0252887E-4	45.0	14
GGCTACG	20	7.0252887E-4	45.0	1
CTAGCGG	20	7.0252887E-4	45.0	2
CGTTTTT	6785	0.0	44.30361	1
GATGAAT	540	0.0	42.916664	20
CCGATGA	510	0.0	42.794117	18
CGATGAA	520	0.0	42.40385	19
TACGGCT	700	0.0	41.464287	7
ACGGCTG	705	0.0	41.17021	8
ACCACCG	680	0.0	41.02941	14
AGCGACT	55	6.002665E-11	40.909092	19
GGCGACT	205	0.0	40.609756	10
>>END_MODULE