FastQCFastQC Report
Sat 18 Jun 2016
SRR3552128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341280
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177885.2121425222691045No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC92612.713607594936709No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG91042.6676043131739333No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC81302.3822081575246132No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC22020.6452180028129395TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT21540.6311533052039381No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTC19400.5684481950304735No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT17680.5180496952648851No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA17360.5086732301922176No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC9040.2648851383028598No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT6230.18254805438349742No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT5400.15822784810126583TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT5150.15090248476324425No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGT4750.13918190342240977No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC4120.12072198781059541TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATG4040.1183778715424285No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT3910.1145686826066573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCT453.8380676E-1045.00000412
AGGGTAC351.208573E-745.06
TCTACGG207.026309E-445.02
GGTTCAT207.026309E-445.08
GCATGCG207.026309E-445.01
TGTACGG253.8850234E-544.9999962
TCAACGG253.8850234E-544.9999962
CGTTTTT76900.043.9466821
TGATACC9800.041.096944
TACGGCT9550.040.9947667
TAGGGTA501.0768417E-940.4999965
GATACCT9950.040.251265
CGGCTGT9750.039.923089
ACGGCTG9850.039.746198
GAATGAC2100.039.642861
GAATGAT31050.039.2753641
AATGATA29700.039.0909082
ATGATAC29650.039.0809443
ATACCTG10250.039.073176
AAGGGCG750.039.0000045
Produced by FastQC (version 0.11.5)