##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341280
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.30855602437881	30.0	27.0	33.0	16.0	33.0
2	28.824197140178153	31.0	28.0	33.0	16.0	33.0
3	28.640981012658226	31.0	28.0	33.0	16.0	34.0
4	32.2815664556962	35.0	32.0	35.0	28.0	37.0
5	33.67455168776371	35.0	33.0	35.0	30.0	37.0
6	33.521993670886076	35.0	33.0	35.0	28.0	37.0
7	34.233822667604315	35.0	33.0	36.0	32.0	37.0
8	34.21357243319269	35.0	35.0	37.0	31.0	37.0
9	36.083986755743084	39.0	35.0	39.0	30.0	39.0
10	36.087391584622594	37.0	35.0	39.0	32.0	39.0
11	36.24816279887482	37.0	35.0	39.0	32.0	39.0
12	36.16640881387717	37.0	35.0	39.0	32.0	39.0
13	36.187968823253634	37.0	35.0	39.0	32.0	39.0
14	37.402141936240035	39.0	36.0	40.0	32.0	41.0
15	37.284719292076886	39.0	36.0	41.0	32.0	41.0
16	37.23778422409752	39.0	36.0	41.0	32.0	41.0
17	36.30766233005157	38.0	34.0	40.0	29.0	41.0
18	35.867865096108765	38.0	34.0	39.0	29.0	40.0
19	35.29194796061885	37.0	34.0	39.0	29.0	40.0
20	35.35011720581341	36.0	34.0	39.0	30.0	40.0
21	35.942935419596814	37.0	35.0	39.0	31.0	40.0
22	36.356279301453355	37.0	35.0	40.0	32.0	41.0
23	36.56867088607595	38.0	35.0	40.0	32.0	41.0
24	36.46531586966714	38.0	35.0	40.0	32.0	41.0
25	35.87136075949367	37.0	35.0	40.0	31.0	41.0
26	35.97920768870136	37.0	35.0	40.0	31.0	41.0
27	36.32720932958275	38.0	35.0	40.0	32.0	41.0
28	36.1781792076887	38.0	35.0	40.0	31.0	41.0
29	36.16143635724332	38.0	35.0	40.0	31.0	41.0
30	35.75346050164088	37.0	35.0	40.0	30.0	41.0
31	35.54061181434599	37.0	35.0	40.0	30.0	41.0
32	35.129491912798876	38.0	35.0	40.0	25.0	41.0
33	34.43747948898265	38.0	34.0	40.0	21.0	41.0
34	33.98731833098922	38.0	34.0	40.0	17.0	41.0
35	33.47433778715424	37.0	33.0	40.0	15.0	41.0
36	33.279081692451946	37.0	33.0	40.0	11.0	41.0
37	33.1221958509142	37.0	33.0	40.0	10.0	41.0
38	32.9291197843413	37.0	33.0	40.0	10.0	41.0
39	32.83805086732302	37.0	33.0	40.0	10.0	41.0
40	32.40491092358181	37.0	31.0	40.0	10.0	41.0
41	32.29011954992968	36.0	31.0	40.0	10.0	41.0
42	32.31685419596812	36.0	31.0	40.0	10.0	41.0
43	32.38060536802625	37.0	31.0	40.0	10.0	41.0
44	32.32712435536803	37.0	31.0	40.0	10.0	41.0
45	32.31580813408345	36.0	31.0	40.0	10.0	41.0
46	32.11190811064229	36.0	31.0	40.0	10.0	41.0
47	32.01385958743554	36.0	31.0	40.0	10.0	41.0
48	32.021381270511014	36.0	31.0	40.0	10.0	41.0
49	31.977080403187998	36.0	31.0	40.0	10.0	41.0
50	31.93064345991561	35.0	31.0	40.0	10.0	41.0
51	30.080971636193155	34.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	9.0
10	13.0
11	7.0
12	8.0
13	13.0
14	18.0
15	29.0
16	89.0
17	214.0
18	459.0
19	630.0
20	999.0
21	1440.0
22	2145.0
23	3186.0
24	4865.0
25	7916.0
26	11185.0
27	12781.0
28	11966.0
29	10870.0
30	10968.0
31	11936.0
32	14275.0
33	19271.0
34	25990.0
35	35050.0
36	38246.0
37	52007.0
38	50827.0
39	13856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71987810595405	11.437822315986873	16.191104078762304	32.65119549929676
2	41.25673933427098	28.769045944678854	17.899964838255976	12.074249882794188
3	21.786216596343177	27.613689639006093	39.13121190811064	11.468881856540085
4	20.079992967651194	16.77361697140178	50.46325597749648	12.68313408345054
5	25.591889357712144	19.87810595405532	38.701945616502584	15.828059071729959
6	17.494432723863103	31.903129395218006	39.97216361931552	10.630274261603375
7	54.39023675574308	4.556375996249415	37.168307548054386	3.885079699953118
8	52.440225035161745	11.529242850445382	30.68008673230192	5.350445382090951
9	48.544889826535396	5.581633849038912	30.657817627754337	15.215658696671355
10	31.950597749648384	17.076593999062354	37.255918893577125	13.716889357712143
11	25.012599624941394	16.882911392405063	41.86855368026254	16.235935302391
12	21.142463666197845	15.017580872011251	45.573722456633845	18.266233005157055
13	18.233122362869196	17.230426629160807	50.8090131270511	13.727437880918893
14	16.76658462259728	22.409751523675574	43.23019221753399	17.593471636193154
15	13.767287857477731	17.79184247538678	52.8524964838256	15.588373183309892
16	16.0196905766526	19.654828879512422	42.37048757618378	21.954992967651197
17	16.010900140646974	19.078762306610408	45.81897562119081	19.091361931551805
18	16.63326300984529	19.25515705578997	45.60800515705579	18.503574777308955
19	16.101734646038445	21.146272855133617	42.53311064228786	20.218881856540087
20	17.721812001875293	20.334036568213783	47.24009610876699	14.70405532114393
21	17.289322550398502	24.51388888888889	42.89381153305204	15.302977027660573
22	15.974566338490389	17.947726207219876	44.5027543366151	21.574953117674635
23	16.136603375527425	23.76347866854196	43.74384669479606	16.356071261134552
24	19.73863103609939	18.031821378340364	41.35284810126582	20.87669948429442
25	15.014943741209564	25.680965775902486	40.58075480543835	18.7233356774496
26	15.283052039381154	18.74970698546648	43.84112751992499	22.12611345522738
27	18.34915611814346	21.59077590248476	40.47204641350211	19.588021565869667
28	14.477848101265822	19.77232770745429	45.68155180496953	20.068272386310362
29	18.433251289263946	18.394573370839193	39.455872011251756	23.7163033286451
30	16.97784810126582	25.5098452883263	41.8398382559775	15.67246835443038
31	23.591772151898734	18.49244022503516	37.55362165963432	20.362165963431785
32	19.630801687763714	23.328645100797	37.53457571495546	19.505977496483826
33	18.50796999531177	24.44590951711205	35.38179793717768	21.6643225503985
34	23.28410689170183	20.73400140646976	35.77795358649789	20.20393811533052
35	16.65992733239569	26.26992498827942	36.193741209563996	20.8764064697609
36	21.2570323488045	27.409458509142056	32.114392873886544	19.2191162681669
37	19.16813173933427	25.925632911392405	35.49490154711673	19.411333802156587
38	20.354254571026722	27.608122362869196	31.38888888888889	20.64873417721519
39	24.02777777777778	23.062001875293014	32.416490857946556	20.493729488982655
40	19.124179559306143	25.026664322550396	37.60196905766526	18.2471870604782
41	20.31645569620253	27.926336146272856	32.24507735583685	19.512130801687764
42	18.52174167838725	22.365799343647446	40.16408813877168	18.948370839193622
43	20.67334739803094	24.910337552742618	32.630684481950304	21.785630567276137
44	20.426629160806375	22.11703000468823	34.920300046882325	22.536040787623065
45	19.3272386310361	20.581633849038912	35.32964135021097	24.76148616971402
46	23.687587904360054	24.486931551804968	33.41479137365213	18.41068917018284
47	16.67164791373652	21.703293483356774	42.645042194092824	18.980016408813878
48	19.57512892639475	23.343295827473042	34.872831692451946	22.208743553680264
49	18.625175808720112	19.69116268166901	41.21308016877637	20.470581340834507
50	19.128867791842474	19.82272620721988	38.39574542897328	22.65266057196437
51	18.23019221753399	19.895979840600095	34.95751289263948	26.91631504922644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	348.0
1	674.5
2	1001.0
3	14929.5
4	28858.0
5	18153.5
6	7449.0
7	7008.0
8	6567.0
9	6268.0
10	5969.0
11	5731.5
12	5494.0
13	5182.5
14	4871.0
15	4533.0
16	4195.0
17	3862.5
18	3530.0
19	3212.0
20	2894.0
21	2866.5
22	2839.0
23	2690.0
24	2541.0
25	2636.5
26	2853.5
27	2975.0
28	3151.5
29	3328.0
30	3592.0
31	3856.0
32	4245.0
33	4634.0
34	5421.5
35	6209.0
36	6462.5
37	6716.0
38	7091.5
39	7467.0
40	8463.5
41	9460.0
42	10608.0
43	11756.0
44	13194.5
45	14633.0
46	33819.0
47	53005.0
48	38218.0
49	23431.0
50	23106.5
51	22782.0
52	19685.0
53	16588.0
54	15483.0
55	14378.0
56	13200.0
57	12022.0
58	11552.0
59	11082.0
60	10949.0
61	10816.0
62	9827.5
63	8839.0
64	7761.0
65	6683.0
66	5536.5
67	4390.0
68	3722.5
69	3055.0
70	2403.5
71	1752.0
72	1352.5
73	953.0
74	774.0
75	440.5
76	286.0
77	216.0
78	146.0
79	104.5
80	63.0
81	53.5
82	44.0
83	29.0
84	14.0
85	12.0
86	10.0
87	10.0
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	3.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.548618903089896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21467549572091	32.49711744800551
2	7.9013552603166755	6.5658079705764445
3	3.4022615747619103	4.240778087352274
4	2.219433777704813	3.6885763284200963
5	1.5598374948823004	3.2404546812807564
6	1.249802648281048	3.115654436250106
7	1.0088951582023966	2.934274030892678
8	0.8388155098040067	2.788130075747819
9	0.6435525468685626	2.4064847562559324
>10	2.860625155819134	17.909271685271204
>50	0.05817690821361875	1.6165562576571986
>100	0.033345301049269284	2.2933639103889507
>500	0.002837897961639939	0.761113832470986
>1k	0.0035473724520499236	2.8888131519038196
>5k	0.0021284234712299543	7.810112699968541
>10k+	7.094744904099847E-4	5.2434906475576675
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17788	5.2121425222691045	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	9261	2.713607594936709	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	9104	2.6676043131739333	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	8130	2.3822081575246132	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	2202	0.6452180028129395	TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	2154	0.6311533052039381	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTC	1940	0.5684481950304735	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	1768	0.5180496952648851	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA	1736	0.5086732301922176	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC	904	0.2648851383028598	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	623	0.18254805438349742	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	540	0.15822784810126583	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT	515	0.15090248476324425	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGT	475	0.13918190342240977	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	412	0.12072198781059541	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATG	404	0.1183778715424285	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	391	0.1145686826066573	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1936826066572902	0.0
2	0.0	0.0	0.0	1.220405532114393	0.0
3	0.0	0.0	0.0	1.4448546647913736	0.0
4	0.0	0.0	0.0	2.3857243319268635	0.0
5	0.0	0.0	0.0	5.981598687294889	0.0
6	0.0	0.0	0.0	6.5726090014064695	0.0
7	0.0	0.0	0.0	7.555379746835443	0.0
8	0.0	0.0	0.0	8.768459915611814	0.0
9	0.0	0.0	0.0	9.067920768870136	0.0
10	0.0	0.0	0.0	12.511427566807313	0.0
11	0.0	0.0	0.0	13.514416315049226	0.0
12	0.0	0.0	0.0	16.898441162681667	0.0
13	0.0	0.0	0.0	17.353785747773088	0.0
14	0.0	0.0	0.0	17.59815986872949	0.0
15	0.0	0.0	0.0	18.389006094702296	0.0
16	0.0	0.0	0.0	18.91906938584154	0.0
17	0.0	0.0	0.0	19.42539849976559	0.0
18	0.0	0.0	0.0	19.961908110642288	0.0
19	0.0	0.0	0.0	20.498417721518987	0.0
20	0.0	0.0	0.0	20.872304266291607	0.0
21	0.0	0.0	0.0	21.252051101734647	0.0
22	0.0	0.0	0.0	21.67721518987342	0.0
23	0.0	0.0	0.0	22.032348804500703	0.0
24	0.0	0.0	0.0	22.32946554149086	0.0
25	0.0	0.0	0.0	22.589369432723863	0.0
26	0.0	0.0	0.0	22.856012658227847	0.0
27	0.0	0.0	0.0	23.12793014533521	0.0
28	0.0	0.0	0.0	23.38373183309892	0.0
29	0.0	0.0	0.0	23.65184013127051	0.0
30	0.0	0.0	0.0	23.95393811533052	0.0
31	0.0	0.0	0.0	24.2261486169714	0.0
32	2.9301453352086266E-4	0.0	0.0	24.496601031411156	0.0
33	2.9301453352086266E-4	0.0	0.0	24.759728082512893	0.0
34	2.9301453352086266E-4	0.0	0.0	25.02080403187998	0.0
35	2.9301453352086266E-4	0.0	0.0	25.293893577121427	0.0
36	2.9301453352086266E-4	0.0	0.0	25.551160337552744	0.0
37	2.9301453352086266E-4	0.0	0.0	25.801394749179558	0.0
38	2.9301453352086266E-4	0.0	0.0	26.082395686826068	0.0
39	5.860290670417253E-4	0.0	0.0	26.397679324894515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCCT	45	3.8380676E-10	45.000004	12
AGGGTAC	35	1.208573E-7	45.0	6
TCTACGG	20	7.026309E-4	45.0	2
GGTTCAT	20	7.026309E-4	45.0	8
GCATGCG	20	7.026309E-4	45.0	1
TGTACGG	25	3.8850234E-5	44.999996	2
TCAACGG	25	3.8850234E-5	44.999996	2
CGTTTTT	7690	0.0	43.946682	1
TGATACC	980	0.0	41.09694	4
TACGGCT	955	0.0	40.994766	7
TAGGGTA	50	1.0768417E-9	40.499996	5
GATACCT	995	0.0	40.25126	5
CGGCTGT	975	0.0	39.92308	9
ACGGCTG	985	0.0	39.74619	8
GAATGAC	210	0.0	39.64286	1
GAATGAT	3105	0.0	39.275364	1
AATGATA	2970	0.0	39.090908	2
ATGATAC	2965	0.0	39.080944	3
ATACCTG	1025	0.0	39.07317	6
AAGGGCG	75	0.0	39.000004	5
>>END_MODULE