FastQCFastQC Report
Sat 18 Jun 2016
SRR3552127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384607
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123433.209249961649164No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC90012.3403110187802096No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG85052.2113482073909214No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC61161.590194666243724No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT26340.6848549298374705No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC24520.6375338982389815TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTA17360.45136983986250906No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCT15330.3985886892334253No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCACTGT14800.3848083888228764No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTC12940.33644733455189324No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC10690.2779460592240911No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT6940.1804439336777542No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGT5000.13000283406178256No Hit
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT4420.11492250531061578TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATG4240.1102424032843916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGC551.8189894E-1245.0000044
CGAACCC253.8859493E-545.034
CACGGGC502.1827873E-1145.04
ACGAACG207.0274266E-445.01
CGTTTTT62750.043.7091671
CCGATGA6600.043.29545218
CGATGAA6250.043.19999719
GATGAAT6450.042.5581420
CGGCGAA556.002665E-1140.90909231
ATGAATG6600.040.9090921
CGCACGG501.0768417E-940.52
ACGGCTG8000.040.218758
CGAATGG451.9224899E-840.02
CGATACC451.9224899E-840.034
GATACCT11600.039.7629285
TGATACC11700.039.6153874
CACCGAT7300.039.45205716
ACCGGTG403.4502227E-739.37517
GGGTATA403.4502227E-739.3757
CCGGTGA403.4502227E-739.37518