##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384607
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.42443585270159	28.0	16.0	31.0	16.0	33.0
2	26.3876736512856	30.0	25.0	31.0	16.0	33.0
3	26.704407356080363	30.0	25.0	33.0	16.0	33.0
4	29.889110182601982	32.0	28.0	35.0	19.0	37.0
5	32.1304162430741	33.0	32.0	35.0	28.0	37.0
6	30.759575358742822	35.0	28.0	35.0	17.0	37.0
7	32.871926407995694	35.0	32.0	35.0	28.0	37.0
8	33.14672899869217	35.0	33.0	37.0	28.0	37.0
9	34.89880319390963	37.0	34.0	39.0	28.0	39.0
10	34.94527400697335	37.0	34.0	39.0	30.0	39.0
11	35.15910006838149	37.0	34.0	39.0	30.0	39.0
12	35.06361298676311	37.0	34.0	39.0	27.0	39.0
13	35.26826864825653	37.0	34.0	39.0	30.0	39.0
14	35.679660016588365	38.0	34.0	40.0	27.0	41.0
15	36.122007659766986	38.0	34.0	40.0	30.0	41.0
16	35.87029357239988	38.0	34.0	40.0	27.0	41.0
17	35.43840335719319	37.0	33.0	40.0	27.0	41.0
18	35.295896850551344	37.0	33.0	39.0	27.0	40.0
19	34.66248924226548	37.0	32.0	39.0	27.0	40.0
20	34.73628405099231	35.0	33.0	39.0	27.0	40.0
21	35.32149960869147	37.0	34.0	39.0	30.0	40.0
22	35.9169593897147	37.0	34.0	39.0	30.0	40.0
23	36.37109828994272	38.0	35.0	40.0	31.0	41.0
24	36.31217840548924	38.0	35.0	40.0	31.0	41.0
25	34.6959363714129	36.0	33.0	39.0	26.0	40.0
26	35.1868114724901	37.0	33.0	39.0	29.0	40.0
27	35.67072102171827	37.0	35.0	40.0	30.0	41.0
28	35.633371207492324	37.0	35.0	40.0	30.0	41.0
29	35.87288062879771	38.0	35.0	40.0	30.0	41.0
30	34.85260278674075	37.0	34.0	40.0	25.0	41.0
31	34.871565000117	37.0	34.0	40.0	27.0	41.0
32	34.512504972608404	37.0	34.0	40.0	24.0	41.0
33	34.370188270104286	37.0	34.0	40.0	22.0	41.0
34	34.22588512429571	38.0	34.0	40.0	18.0	41.0
35	33.999693193311614	38.0	34.0	40.0	15.0	41.0
36	33.67529452142057	38.0	33.0	40.0	15.0	41.0
37	33.7578047201429	38.0	34.0	40.0	14.0	41.0
38	33.41339341197638	37.0	33.0	40.0	14.0	41.0
39	33.307792109868	37.0	33.0	40.0	12.0	41.0
40	32.94132192081788	37.0	32.0	40.0	12.0	41.0
41	32.779200586572784	37.0	32.0	40.0	13.0	41.0
42	33.0138141011474	37.0	33.0	40.0	11.0	41.0
43	33.018855611052324	37.0	33.0	40.0	10.0	41.0
44	33.20946576635371	37.0	33.0	40.0	11.0	41.0
45	33.214034065942634	37.0	33.0	40.0	11.0	41.0
46	33.01521293164191	37.0	33.0	40.0	10.0	41.0
47	32.89672314856464	36.0	32.0	40.0	11.0	41.0
48	32.93538079130125	36.0	33.0	40.0	11.0	41.0
49	33.04675421924198	36.0	33.0	40.0	11.0	41.0
50	32.969158127647184	36.0	33.0	40.0	10.0	41.0
51	31.839781907245577	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	17.0
11	23.0
12	20.0
13	26.0
14	40.0
15	57.0
16	113.0
17	209.0
18	436.0
19	908.0
20	1679.0
21	2531.0
22	3371.0
23	4510.0
24	6180.0
25	9034.0
26	11311.0
27	11823.0
28	11215.0
29	11038.0
30	12724.0
31	16277.0
32	21463.0
33	27158.0
34	33807.0
35	49983.0
36	59513.0
37	57610.0
38	28736.0
39	2771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57020803053507	12.969602737339676	16.85330740210137	27.606881830023895
2	38.71120390424511	26.808404423216427	18.868871341395245	15.61152033114322
3	24.742139378638452	26.129529623745796	34.92838143871536	14.199949558900386
4	21.439547382132933	18.685047334031882	44.277405247434395	15.598000036400794
5	26.81594458759201	21.57813040324279	34.50301216566521	17.102912843499986
6	20.09453806092973	31.17597963635607	35.37507117655165	13.35441112616255
7	60.16609162079733	4.399555910318845	31.616949249493636	3.817403219390183
8	59.03766702114106	11.137082788404786	24.357070984147455	5.468179206306697
9	54.22600212684636	5.119511605352996	24.99486488805456	15.659621379746078
10	34.48117168954283	18.90527213493254	31.841854152420524	14.771702023104103
11	27.393677182162573	19.191798381204713	36.60463798110799	16.80988645552473
12	20.531607589045443	17.76514728021071	40.986253500326306	20.716991630417546
13	21.321244803136707	19.254199741554366	44.77687613589976	14.647679319409162
14	17.88136981386194	24.218747968705717	39.402558975785674	18.49732324164667
15	15.550679004802303	19.258619837912466	46.25318831950537	18.937512837779863
16	18.2968588715234	20.969197128497402	37.62126014347113	23.11268385650807
17	18.80386992436435	22.05004069088706	39.81960806745587	19.326481317292718
18	19.891733639793348	19.21363885732709	39.921530289360305	20.973097213519253
19	18.45286227239754	21.587230601627116	37.41949574500724	22.540411380968106
20	21.630911553871872	21.401846560255013	41.56138603821563	15.40585584765748
21	19.44920399264704	25.52553645669476	38.753324822481126	16.271934728177072
22	18.765649091150188	18.37070048127049	41.10689613033564	21.75675429724368
23	19.048015246732376	24.23876840515123	39.42231940656306	17.290896941553328
24	19.5966272064731	22.0250801467472	37.55339866409088	20.82489398268882
25	17.42167979261948	27.267054421786398	36.16132831695731	19.149937468636814
26	17.89515011427249	19.54618610685713	41.041114696300376	21.51754908257
27	22.110102000223606	20.864674849911726	36.8420231561048	20.183199993759864
28	15.915466957179666	22.3693276513428	41.34454131100058	20.370664080476956
29	20.08439783987291	19.62574784130294	40.51200316166892	19.777851157155226
30	20.880275189999143	23.433010839636303	38.67896320139779	17.007750768966766
31	20.290322329026772	19.38004248492617	38.22265325384093	22.10698193220612
32	21.73335378711256	22.72137532598211	35.82098089738356	19.724289989521772
33	20.920576068558294	22.4189887339544	33.10444167682856	23.55599352065875
34	18.88317165314204	21.218022552891654	37.55495869809962	22.34384709586669
35	22.107501943542367	22.138702623717197	35.3657109724992	20.38808446024123
36	22.470989867579114	24.37553138658423	34.93722163143156	18.216257114405092
37	21.167321447607556	22.727875467685195	34.16500479710457	21.93979828760267
38	20.303062606764826	26.35287449266394	31.9188158301851	21.425247070386135
39	22.18862371199692	22.439529181736162	30.71758964345423	24.65425746281269
40	21.381306112473254	23.708876853515406	35.098165140000056	19.81165189401129
41	21.82591580496455	23.71147691019664	31.14737901286248	23.31522827197633
42	21.231282841965953	21.760654382265535	36.61503820783293	20.39302456793558
43	24.424152446523333	22.196943893376876	31.411284766007896	21.967618894091892
44	22.706034991562817	21.34022521690973	32.17908150397679	23.77465828755067
45	20.42500526511478	20.933316346296348	33.67125403333793	24.970424355250945
46	24.167266846417252	23.739297516685866	31.525687259982266	20.567748376914615
47	18.321039398658893	23.455631332763055	38.55806056572033	19.665268702857723
48	20.721411726775642	23.66623592394314	32.39514621418747	23.217206135093747
49	20.62780968625116	20.64731011136043	37.20811113682278	21.51676906556563
50	20.22272085531465	21.58541056195025	34.60805445558714	23.583814127147974
51	20.029536643898837	20.755212463631707	32.7607141835692	26.454536708900257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	458.5
2	762.0
3	10282.0
4	19802.0
5	12917.5
6	6033.0
7	5627.5
8	5222.0
9	5152.0
10	5082.0
11	4931.0
12	4780.0
13	4520.0
14	4260.0
15	3984.0
16	3708.0
17	3483.5
18	3259.0
19	3073.5
20	2888.0
21	2786.5
22	2685.0
23	2767.0
24	2849.0
25	2841.5
26	3169.0
27	3504.0
28	3849.5
29	4195.0
30	4705.5
31	5216.0
32	5763.5
33	6311.0
34	6977.5
35	7644.0
36	8391.0
37	9138.0
38	9622.0
39	10106.0
40	10593.0
41	11080.0
42	12501.5
43	13923.0
44	15436.0
45	16949.0
46	34526.5
47	52104.0
48	40565.0
49	29026.0
50	28670.5
51	28315.0
52	25664.5
53	23014.0
54	21999.0
55	20984.0
56	19392.5
57	17801.0
58	16305.0
59	14809.0
60	13382.5
61	11956.0
62	11120.0
63	10284.0
64	9357.5
65	8431.0
66	7134.0
67	5837.0
68	4951.5
69	4066.0
70	3273.5
71	2481.0
72	1949.5
73	1418.0
74	1145.5
75	684.5
76	496.0
77	347.5
78	199.0
79	138.0
80	77.0
81	54.0
82	31.0
83	21.0
84	11.0
85	8.0
86	5.0
87	3.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	384607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.95612090878963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.22530224314346	34.267939039517906
2	8.556932527719677	7.693729866490413
3	4.08480460146389	5.509109086565727
4	2.777395024867641	4.994436261976818
5	1.9623743764391344	4.411036986775419
6	1.5532308507304997	4.189634035482151
7	1.127844724891005	3.5492466672978416
8	0.8530936119069913	3.06814236507254
9	0.6694382958709426	2.7085814073133356
>10	2.1190743773926304	13.455759626802601
>50	0.03995530715865048	1.2483837556026223
>100	0.02350312185802969	1.9983121267739818
>500	5.875780464507423E-4	0.18332169411515445
>1k	0.004113046325155196	3.222129718755985
>5k	0.001762734139352227	6.239805559637144
>10k+	5.875780464507423E-4	3.260431801820391
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12343	3.209249961649164	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	9001	2.3403110187802096	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	8505	2.2113482073909214	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	6116	1.590194666243724	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	2634	0.6848549298374705	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	2452	0.6375338982389815	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTA	1736	0.45136983986250906	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCT	1533	0.3985886892334253	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCACTGT	1480	0.3848083888228764	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTC	1294	0.33644733455189324	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC	1069	0.2779460592240911	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	694	0.1804439336777542	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGT	500	0.13000283406178256	No Hit
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	442	0.11492250531061578	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATG	424	0.1102424032843916	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11804257332809855	0.0
2	0.0	0.0	0.0	0.9503207169916305	0.0
3	0.0	0.0	0.0	1.1367447810362266	0.0
4	0.0	0.0	0.0	2.0883655263684746	0.0
5	0.0	0.0	0.0	5.080770760802586	0.0
6	0.0	0.0	0.0	5.693084109233581	0.0
7	0.0	0.0	0.0	6.512101963822811	0.0
8	0.0	0.0	0.0	7.446302329390781	0.0
9	0.0	0.0	0.0	7.775989516571461	0.0
10	0.0	0.0	0.0	10.721853736411454	0.0
11	0.0	0.0	0.0	11.657874141656288	0.0
12	0.0	0.0	0.0	14.091007183956611	0.0
13	0.0	0.0	0.0	14.531196780089806	0.0
14	0.0	0.0	0.0	14.754541649007948	0.0
15	0.0	0.0	0.0	15.441216618522283	0.0
16	0.0	0.0	0.0	15.911566872157813	0.0
17	0.0	0.0	0.0	16.38607721648332	0.0
18	0.0	0.0	0.0	16.867087702511913	0.0
19	0.0	0.0	0.0	17.5808032615111	0.0
20	0.0	0.0	0.0	17.95781148029027	0.0
21	0.0	0.0	0.0	18.293218792169668	0.0
22	0.0	0.0	0.0	18.711307906512364	0.0
23	0.0	0.0	0.0	19.031634889640593	0.0
24	0.0	0.0	0.0	19.292160569100407	0.0
25	0.0	0.0	0.0	19.538385936813423	0.0
26	0.0	0.0	0.0	19.800991661618223	0.0
27	0.0	0.0	0.0	20.101298208300943	0.0
28	0.0	0.0	0.0	20.35194367237206	0.0
29	2.600056681235651E-4	0.0	0.0	20.601809119438805	0.0
30	2.600056681235651E-4	0.0	0.0	20.9195360458858	0.0
31	2.600056681235651E-4	0.0	0.0	21.17122153262941	0.0
32	2.600056681235651E-4	0.0	0.0	21.46216787525968	0.0
33	2.600056681235651E-4	0.0	0.0	21.726593639741345	0.0
34	2.600056681235651E-4	0.0	0.0	21.983999251183675	0.0
35	2.600056681235651E-4	0.0	0.0	22.236464754931657	0.0
36	2.600056681235651E-4	0.0	0.0	22.483210133980922	0.0
37	2.600056681235651E-4	0.0	0.0	22.72579542234021	0.0
38	2.600056681235651E-4	0.0	0.0	23.00972161193114	0.0
39	2.600056681235651E-4	0.0	0.0	23.42235060724324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGC	55	1.8189894E-12	45.000004	4
CGAACCC	25	3.8859493E-5	45.0	34
CACGGGC	50	2.1827873E-11	45.0	4
ACGAACG	20	7.0274266E-4	45.0	1
CGTTTTT	6275	0.0	43.709167	1
CCGATGA	660	0.0	43.295452	18
CGATGAA	625	0.0	43.199997	19
GATGAAT	645	0.0	42.55814	20
CGGCGAA	55	6.002665E-11	40.909092	31
ATGAATG	660	0.0	40.90909	21
CGCACGG	50	1.0768417E-9	40.5	2
ACGGCTG	800	0.0	40.21875	8
CGAATGG	45	1.9224899E-8	40.0	2
CGATACC	45	1.9224899E-8	40.0	34
GATACCT	1160	0.0	39.762928	5
TGATACC	1170	0.0	39.615387	4
CACCGAT	730	0.0	39.452057	16
ACCGGTG	40	3.4502227E-7	39.375	17
GGGTATA	40	3.4502227E-7	39.375	7
CCGGTGA	40	3.4502227E-7	39.375	18
>>END_MODULE