FastQCFastQC Report
Sat 18 Jun 2016
SRR3552124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360461
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129393.5895700228318734No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG126483.5088400686898167No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC106912.965924191521413No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC68901.9114411822638233No Hit
GAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT26380.7318406152121866RNA PCR Primer, Index 23 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT24530.6805174484895731No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTC22080.6125489303974633No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC21930.6083875925550892RNA PCR Primer, Index 23 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTA16290.4519212896818241No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC9610.26660304443476546No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT6840.18975700561225764No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTGGATAT5460.15147269746241618No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA5360.1486984722341668No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT5310.1473113596200421RNA PCR Primer, Index 30 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTGGATATCGT4480.12428529022557226No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC4260.11818199472342361RNA PCR Primer, Index 30 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTAT4090.11346581183539967No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC3700.10264633344522708No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATG3630.10070437578545252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC302.1613414E-645.00000425
TCGCAGG253.8854592E-545.0000042
ACACGAC302.1613414E-645.00000426
GTACATG253.8854592E-545.0000041
TCTACGG302.1613414E-645.0000042
TACGAAT253.8854592E-545.00000412
CGGTCTA253.8854592E-545.00000431
GCGTAAG253.8854592E-545.0000041
ACGGTCT253.8854592E-545.00000430
GCGCCCA207.026837E-445.021
AACGGGC406.7921064E-945.04
TTAAGCG207.026837E-445.01
CTTGACA207.026837E-445.014
TAGTACG207.026837E-445.01
CGTTTTT59850.043.8345871
GGCGACT1550.042.09677510
ACGGCTG8150.041.963198
TACGGCT8200.041.7073177
GATACCT13900.041.600725
TGATACC13950.041.4516144
Produced by FastQC (version 0.11.5)