##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360461
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.093191773867353	30.0	25.0	33.0	16.0	33.0
2	28.56881881812457	31.0	28.0	33.0	16.0	33.0
3	28.480029739694448	31.0	28.0	33.0	16.0	34.0
4	32.47415947911147	35.0	32.0	35.0	28.0	37.0
5	33.645048978946406	35.0	33.0	35.0	30.0	37.0
6	33.40428506828756	35.0	33.0	35.0	28.0	37.0
7	34.15897975093006	35.0	33.0	36.0	32.0	37.0
8	34.134039466128094	35.0	35.0	37.0	30.0	37.0
9	35.95711880064695	39.0	35.0	39.0	30.0	39.0
10	35.83041438602234	37.0	35.0	39.0	31.0	39.0
11	36.082813397288476	37.0	35.0	39.0	31.0	39.0
12	36.03746591170751	37.0	35.0	39.0	31.0	39.0
13	36.073220126449186	37.0	35.0	39.0	31.0	39.0
14	37.28028552326049	39.0	36.0	40.0	32.0	41.0
15	37.156605014134676	39.0	36.0	40.0	31.0	41.0
16	37.10646921580975	39.0	36.0	40.0	32.0	41.0
17	36.16864515162528	38.0	34.0	40.0	29.0	41.0
18	35.811904755299466	38.0	34.0	39.0	29.0	41.0
19	35.29682822829654	37.0	34.0	39.0	27.0	40.0
20	35.423011088578235	37.0	34.0	39.0	29.0	40.0
21	36.06869259087668	37.0	35.0	39.0	31.0	40.0
22	36.50658184935402	38.0	35.0	40.0	32.0	41.0
23	36.73073924779657	38.0	35.0	40.0	32.0	41.0
24	36.64870818202247	38.0	35.0	40.0	32.0	41.0
25	36.04015968440414	38.0	35.0	40.0	31.0	41.0
26	36.16322708975451	38.0	35.0	40.0	31.0	41.0
27	36.52379591689531	38.0	35.0	40.0	32.0	41.0
28	36.34579607780037	38.0	35.0	40.0	31.0	41.0
29	36.36608676111979	38.0	35.0	40.0	31.0	41.0
30	36.00932139676692	38.0	35.0	40.0	30.0	41.0
31	35.75602631075206	38.0	35.0	40.0	30.0	41.0
32	35.552864248836904	38.0	35.0	40.0	28.0	41.0
33	35.08836184774497	38.0	35.0	40.0	23.0	41.0
34	34.645395757099934	38.0	34.0	40.0	21.0	41.0
35	34.496003728558705	38.0	35.0	40.0	18.0	41.0
36	34.127947267526864	38.0	34.0	40.0	16.0	41.0
37	34.07918193646469	38.0	34.0	40.0	15.0	41.0
38	33.84918201969145	38.0	34.0	40.0	15.0	41.0
39	33.74226337939472	38.0	33.0	40.0	15.0	41.0
40	33.57415087901327	38.0	33.0	40.0	15.0	41.0
41	33.301183761904895	38.0	33.0	40.0	12.0	41.0
42	33.44436984861053	38.0	33.0	40.0	12.0	41.0
43	33.46924910045747	38.0	33.0	40.0	12.0	41.0
44	33.417723415293196	38.0	33.0	40.0	12.0	41.0
45	33.34499155248418	37.0	33.0	40.0	12.0	41.0
46	33.2035587761228	37.0	33.0	40.0	12.0	41.0
47	33.11018390339038	37.0	33.0	40.0	12.0	41.0
48	33.069688537733626	37.0	33.0	40.0	12.0	41.0
49	33.042681455136616	37.0	33.0	40.0	12.0	41.0
50	32.967963247064176	37.0	33.0	40.0	12.0	41.0
51	31.055614893150715	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	8.0
10	17.0
11	13.0
12	13.0
13	14.0
14	21.0
15	37.0
16	107.0
17	260.0
18	491.0
19	700.0
20	970.0
21	1525.0
22	2074.0
23	2923.0
24	4482.0
25	7220.0
26	9999.0
27	11091.0
28	10601.0
29	9736.0
30	10046.0
31	11705.0
32	14968.0
33	20276.0
34	26924.0
35	35266.0
36	43325.0
37	59382.0
38	59650.0
39	16611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45396866790027	12.2024296664549	16.885876696785505	28.45772496885932
2	36.77623931576509	31.017225164442202	19.153250975833725	13.053284543958984
3	23.375344350706456	29.223965976901802	35.442946671068434	11.957743001323305
4	21.342114680922485	18.137052274725978	46.451904644330455	14.068928400021083
5	27.455674816415645	21.429780198135166	34.679202465731386	16.435342519717803
6	19.06641772618952	34.032807987549276	35.84604159673307	11.054732689528132
7	58.63630184680173	5.081270928061565	32.17463192966784	4.107795295468858
8	57.26028613359004	11.906697257123517	25.624686165771056	5.208330443515387
9	53.24043377785669	5.734600969314295	25.34587653033199	15.679088722497026
10	32.03453355564125	18.98513292700181	33.07986162164562	15.900471895711323
11	25.02212444619529	18.95017768912587	38.456032691470085	17.571665173208753
12	22.23347324675901	17.26927462332957	41.699101983293616	18.798150146617804
13	18.924099971980326	18.994010447732208	47.03893070262802	15.04295887765944
14	17.043730112272897	24.649268575518573	38.86301153245427	19.443989779754258
15	14.671212697074024	19.706154063823824	49.312130854655564	16.310502384446586
16	16.48722053148607	21.720519002055703	38.711816257514684	23.080444208943547
17	16.690848663239574	21.033898258063978	42.18930758112528	20.085945497571167
18	17.268442355761096	21.298559344838967	42.02091210977054	19.41208618962939
19	17.463470389307027	23.202787541509345	37.54747392921842	21.786268139965212
20	18.401435938978143	22.454578997450486	43.306765503064135	15.83721956050724
21	17.84187471044024	26.40146922968088	39.23864162835924	16.51801443151964
22	16.933038525665744	19.102205231633935	39.48748963133321	24.47726661136711
23	17.44737988298318	25.455458426847844	39.976585539073575	17.120576151095403
24	19.72196714762485	20.497363098920548	37.40127225968968	22.37939749376493
25	16.0169893552978	28.588390977109867	36.281317535045396	19.113302132546934
26	17.079795040240136	20.29512207978117	40.24124662584857	22.383836254130127
27	21.03278856797268	21.829546053525902	36.325982561220215	20.811682817281206
28	15.242980516616223	20.75286924244232	42.814340525049865	21.189809715891595
29	22.055367987105402	18.59618655000125	36.08074105104297	23.26770441185038
30	21.914437345510333	23.343718183104414	37.892310125089814	16.84953434629544
31	20.481272592596703	20.4757241421402	37.291412940651	21.751590324612096
32	26.22336397002727	22.247066950377434	34.43534806816827	17.09422101142703
33	22.35248750905091	21.52105220814457	33.60751926005865	22.51894102274587
34	20.2565603491085	24.08499116409265	34.647853720652165	21.010594766146685
35	23.49075212020163	21.98101875098832	34.28415279322867	20.244076335581383
36	19.491983876202973	26.797351169752066	34.67254432518359	19.038120628861375
37	24.59184211329381	23.519049217529776	35.0365226751299	16.852585994046514
38	23.22220711810709	24.02229367393421	32.66955371038753	20.085945497571167
39	24.395149544610927	26.618136220007155	30.92955964722952	18.057154588152393
40	22.381339451424704	22.43793364608099	37.28503222262602	17.895694679868278
41	18.283531366777545	31.299918715200814	30.684318137052273	19.73223178096937
42	19.735283428720443	22.713691633768978	39.137659830050964	18.413365107459615
43	22.46484363079501	24.24478653723981	31.00085723559553	22.289512596369647
44	22.466230743409135	22.497302065965528	32.85237515292916	22.184092037696175
45	20.090661680459192	21.279139768241222	34.363218212233775	24.266980339065807
46	24.221760467845343	25.761455469523746	31.555147436199753	18.46163662643115
47	18.008883069180854	21.89418550134411	41.1081920096765	18.988739419798538
48	21.479716252243655	23.087102349491346	32.78468405735988	22.64849734090512
49	18.651393632043412	20.790876128069332	38.99589692088742	21.561833318999838
50	19.485325735655177	20.96648458501752	36.12873514749169	23.41945453183562
51	20.770346861380286	21.468896773853483	31.926616194262348	25.834140170503883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185.0
1	528.5
2	872.0
3	10291.0
4	19710.0
5	12860.0
6	6010.0
7	5642.5
8	5275.0
9	5175.0
10	5075.0
11	4938.0
12	4801.0
13	4560.5
14	4320.0
15	4069.0
16	3818.0
17	3510.5
18	3203.0
19	3083.0
20	2963.0
21	3131.5
22	3300.0
23	3033.5
24	2767.0
25	2846.5
26	3126.5
27	3327.0
28	3701.0
29	4075.0
30	4527.5
31	4980.0
32	5237.5
33	5495.0
34	6448.0
35	7401.0
36	7869.5
37	8338.0
38	9141.0
39	9944.0
40	10706.0
41	11468.0
42	13033.0
43	14598.0
44	15873.0
45	17148.0
46	38944.0
47	60740.0
48	43247.0
49	25754.0
50	25133.5
51	24513.0
52	21914.0
53	19315.0
54	17503.5
55	15692.0
56	14436.0
57	13180.0
58	12474.0
59	11768.0
60	10861.5
61	9955.0
62	9203.0
63	8451.0
64	7522.5
65	6594.0
66	5663.0
67	4732.0
68	4027.0
69	3322.0
70	2615.5
71	1909.0
72	1524.5
73	1140.0
74	948.0
75	546.5
76	337.0
77	241.0
78	145.0
79	104.0
80	63.0
81	54.5
82	46.0
83	30.0
84	14.0
85	17.0
86	20.0
87	13.5
88	7.0
89	6.0
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	360461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.88131858642383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20895841455659	30.393137496704565
2	7.828619469071484	6.2443133427584
3	3.5548325235876863	4.253142251837446
4	2.390278116631218	3.81309772318107
5	1.8953308963976834	3.7794147652964143
6	1.5534828870221493	3.7172967561532677
7	1.251285456180797	3.4932039744353527
8	1.0731329217499133	3.42383647503105
9	0.8002732064525923	2.872435563244329
>10	3.373039257250639	19.25065310916502
>50	0.03923706595606636	1.0928632506407645
>100	0.02172051865425102	1.586062851096134
>500	0.0035033094603630676	0.9103933701323474
>1k	0.0035033094603630676	3.1075766326709138
>5k	7.006618920726135E-4	1.9252947575849828
>10k+	0.0021019856762178406	10.137277680067927
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12939	3.5895700228318734	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG	12648	3.5088400686898167	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC	10691	2.965924191521413	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC	6890	1.9114411822638233	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT	2638	0.7318406152121866	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT	2453	0.6805174484895731	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTC	2208	0.6125489303974633	No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2193	0.6083875925550892	RNA PCR Primer, Index 23 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTA	1629	0.4519212896818241	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	961	0.26660304443476546	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT	684	0.18975700561225764	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTGGATAT	546	0.15147269746241618	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	536	0.1486984722341668	No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	531	0.1473113596200421	RNA PCR Primer, Index 30 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTGGATATCGT	448	0.12428529022557226	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	426	0.11818199472342361	RNA PCR Primer, Index 30 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTAT	409	0.11346581183539967	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC	370	0.10264633344522708	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATG	363	0.10070437578545252	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16423413351236332	0.0
2	0.0	0.0	0.0	1.0561475443945392	0.0
3	0.0	0.0	0.0	1.2608853662393436	0.0
4	0.0	0.0	0.0	2.234360998832051	0.0
5	0.0	0.0	0.0	5.968745578578543	0.0
6	0.0	0.0	0.0	6.577688016179281	0.0
7	0.0	0.0	0.0	7.695978205686607	0.0
8	0.0	0.0	0.0	8.86975289975892	0.0
9	0.0	0.0	0.0	9.15965943611098	0.0
10	0.0	0.0	0.0	13.42031454165638	0.0
11	0.0	0.0	0.0	14.319163515609178	0.0
12	0.0	0.0	0.0	17.06425937896194	0.0
13	0.0	0.0	0.0	17.444883080277755	0.0
14	0.0	0.0	0.0	17.685963252612627	0.0
15	0.0	0.0	0.0	18.366203278579373	0.0
16	0.0	0.0	0.0	18.84919589081759	0.0
17	0.0	0.0	0.0	19.278091111104946	0.0
18	2.7742252282493806E-4	0.0	0.0	19.75914176568339	0.0
19	2.7742252282493806E-4	0.0	0.0	20.213837280593463	0.0
20	2.7742252282493806E-4	0.0	0.0	20.515118140381347	0.0
21	2.7742252282493806E-4	0.0	0.0	20.82666363351375	0.0
22	2.7742252282493806E-4	0.0	0.0	21.210061560057817	0.0
23	2.7742252282493806E-4	0.0	0.0	21.527155503646718	0.0
24	2.7742252282493806E-4	0.0	0.0	21.78266164716849	0.0
25	2.7742252282493806E-4	0.0	0.0	22.014586876250135	0.0
26	5.548450456498761E-4	0.0	0.0	22.253725090925233	0.0
27	5.548450456498761E-4	0.0	0.0	22.523657205633896	0.0
28	5.548450456498761E-4	0.0	0.0	22.74753718155362	0.0
29	5.548450456498761E-4	0.0	0.0	22.983068903431995	0.0
30	5.548450456498761E-4	0.0	0.0	23.268536679418855	0.0
31	5.548450456498761E-4	0.0	0.0	23.51433303464175	0.0
32	5.548450456498761E-4	0.0	0.0	23.752638981748373	0.0
33	5.548450456498761E-4	0.0	0.0	23.983177098215897	0.0
34	5.548450456498761E-4	0.0	0.0	24.23063798857574	0.0
35	5.548450456498761E-4	0.0	0.0	24.472550428479085	0.0
36	5.548450456498761E-4	0.0	0.0	24.705585347652036	0.0
37	5.548450456498761E-4	0.0	0.0	24.92280718302396	0.0
38	5.548450456498761E-4	0.0	0.0	25.15639694724256	0.0
39	5.548450456498761E-4	0.0	0.0	25.401638457419804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	2.1613414E-6	45.000004	25
TCGCAGG	25	3.8854592E-5	45.000004	2
ACACGAC	30	2.1613414E-6	45.000004	26
GTACATG	25	3.8854592E-5	45.000004	1
TCTACGG	30	2.1613414E-6	45.000004	2
TACGAAT	25	3.8854592E-5	45.000004	12
CGGTCTA	25	3.8854592E-5	45.000004	31
GCGTAAG	25	3.8854592E-5	45.000004	1
ACGGTCT	25	3.8854592E-5	45.000004	30
GCGCCCA	20	7.026837E-4	45.0	21
AACGGGC	40	6.7921064E-9	45.0	4
TTAAGCG	20	7.026837E-4	45.0	1
CTTGACA	20	7.026837E-4	45.0	14
TAGTACG	20	7.026837E-4	45.0	1
CGTTTTT	5985	0.0	43.834587	1
GGCGACT	155	0.0	42.096775	10
ACGGCTG	815	0.0	41.96319	8
TACGGCT	820	0.0	41.707317	7
GATACCT	1390	0.0	41.60072	5
TGATACC	1395	0.0	41.451614	4
>>END_MODULE