FastQCFastQC Report
Sat 18 Jun 2016
SRR3552123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400332
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128123.200343714716785No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG108022.69826044383162No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC96262.4045042614629857No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC91412.2833548155031322No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC29940.7478792602140224No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT21300.532058391535026No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT20020.5000849295085079No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA19750.4933405273622893No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT17610.4398848955367045No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC14510.36244916719123127No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT8950.22356444151354374No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATG5160.12889301879440065No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGT5160.12889301879440065No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTAT4070.10166561753744392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA302.1619435E-645.00000442
CTTAACG207.027772E-445.01
CGACCCA207.027772E-445.034
CTAAGAC207.027772E-445.018
CGTTAGG253.8862352E-545.02
TGTTGCG253.8862352E-545.01
CTACGAA406.7957444E-945.011
TACGAAT406.7957444E-945.012
CGAATGG253.8862352E-545.02
CTCGATG253.8862352E-545.01
CGATCGA351.2092823E-745.041
AACAGCG207.027772E-445.01
ACGACCC207.027772E-445.036
CGTTTTT69000.043.663041
GCGATCC1000.042.7533
GCGATAC750.042.033
CCGATGA13900.041.9244618
GATGAAT13400.041.80970420
ATGAATG13300.041.78571721
CGATGAA13300.041.78571719