##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400332
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.76815243348021	28.0	16.0	31.0	16.0	33.0
2	26.52142471748449	30.0	25.0	31.0	16.0	33.0
3	26.842768002557875	30.0	25.0	33.0	16.0	33.0
4	29.93121209396201	32.0	28.0	35.0	19.0	37.0
5	32.13689887393463	33.0	32.0	35.0	28.0	37.0
6	30.83964559415685	35.0	29.0	35.0	17.0	37.0
7	32.79867210215521	35.0	32.0	35.0	28.0	37.0
8	32.89678316996893	35.0	32.0	36.0	28.0	37.0
9	34.77513413866491	37.0	33.0	39.0	28.0	39.0
10	34.87056243318046	37.0	34.0	39.0	29.0	39.0
11	35.094881248563695	37.0	34.0	39.0	30.0	39.0
12	35.02142971333793	37.0	34.0	39.0	27.0	39.0
13	35.14054334902031	37.0	34.0	39.0	30.0	39.0
14	35.602120240200634	38.0	34.0	40.0	27.0	41.0
15	36.074925311991045	38.0	34.0	40.0	30.0	41.0
16	35.91502802673781	38.0	34.0	40.0	29.0	41.0
17	35.29595185995623	37.0	33.0	40.0	27.0	41.0
18	35.182775796089246	37.0	33.0	39.0	27.0	40.0
19	34.576224233885874	37.0	32.0	39.0	27.0	40.0
20	34.30548145039617	35.0	33.0	39.0	27.0	40.0
21	35.03071450695922	36.0	34.0	39.0	29.0	40.0
22	35.55374289339848	37.0	34.0	39.0	30.0	40.0
23	35.94825045212474	37.0	35.0	40.0	30.0	41.0
24	35.89866161086298	37.0	35.0	40.0	30.0	41.0
25	34.44080663049669	35.0	33.0	39.0	25.0	40.0
26	34.79057632165303	36.0	33.0	39.0	27.0	40.0
27	35.12751666117123	37.0	34.0	39.0	29.0	40.0
28	34.98528970954108	36.0	34.0	40.0	27.0	41.0
29	35.18684991457091	37.0	34.0	40.0	27.0	41.0
30	34.221586083550655	36.0	33.0	40.0	24.0	41.0
31	34.240560334922016	36.0	33.0	39.0	24.0	40.0
32	33.77822657194528	36.0	33.0	39.0	21.0	40.0
33	33.47654946394493	37.0	33.0	40.0	18.0	41.0
34	33.284823596414974	37.0	33.0	40.0	15.0	41.0
35	32.886289379814755	37.0	33.0	40.0	12.0	41.0
36	32.73889671572595	37.0	32.0	40.0	10.0	41.0
37	32.58009102444971	37.0	32.0	40.0	10.0	41.0
38	32.38926940639269	37.0	31.0	40.0	10.0	41.0
39	32.155463465323784	36.0	31.0	40.0	10.0	41.0
40	32.08627589101046	36.0	31.0	40.0	10.0	41.0
41	31.75024729474536	35.0	30.0	40.0	10.0	41.0
42	31.964721780921835	36.0	31.0	40.0	8.0	41.0
43	32.04451805001848	36.0	31.0	40.0	10.0	41.0
44	32.26657124586593	36.0	31.0	40.0	10.0	41.0
45	32.284901032143324	36.0	32.0	40.0	10.0	41.0
46	32.094703895766514	36.0	31.0	40.0	10.0	41.0
47	31.974850873774766	35.0	31.0	40.0	10.0	41.0
48	32.0317436527682	35.0	31.0	40.0	10.0	41.0
49	32.19343444940699	35.0	31.0	40.0	10.0	41.0
50	32.0235979137316	35.0	31.0	40.0	10.0	41.0
51	31.073621394242778	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	21.0
10	17.0
11	23.0
12	18.0
13	25.0
14	33.0
15	55.0
16	137.0
17	305.0
18	666.0
19	1221.0
20	2119.0
21	3418.0
22	4780.0
23	6187.0
24	7970.0
25	10579.0
26	13193.0
27	14404.0
28	13632.0
29	13488.0
30	15067.0
31	18490.0
32	23674.0
33	29433.0
34	34812.0
35	48743.0
36	55809.0
37	53038.0
38	26490.0
39	2479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82462556078455	12.043254099097748	15.73319145109559	28.398928889022113
2	37.72418892319374	29.924412737427936	17.863673151284434	14.487725188093883
3	22.7386269396401	28.47686420271175	35.71835376637391	13.066155091274242
4	20.441283734500367	17.344104393353515	48.609404194518554	13.60520767762757
5	30.248393833118513	19.725877521657026	34.241079903679946	15.784648741544519
6	18.408720761767732	34.11168729954138	35.026428064706295	12.453163873984593
7	56.536324850374186	4.1038438096380006	35.87647252780192	3.4833588121858856
8	55.44123377596595	14.462496128213584	25.097918727456207	4.998351368364258
9	51.1110778054215	4.700098917898145	25.311241669414386	18.87758160726597
10	36.88113865491642	16.890730693524375	31.863803043473915	14.36432760808529
11	29.947393663259497	17.19572754613671	36.743752685271225	16.113126105332572
12	21.294825295005147	15.362748918397731	40.77091014458	22.571515642017125
13	22.98267438026438	17.20072339957835	45.698070601400836	14.118531618756434
14	17.817461506949233	25.597254278948473	38.408371052026816	18.17691316207548
15	15.504381363468323	16.986651079603927	47.73562942757511	19.773338129352638
16	17.23869188573484	18.97225302998511	38.05941068912803	25.729644395152025
17	17.6413576731313	22.058191700888262	41.49430972292997	18.80614090305047
18	18.458179710840003	18.001558706273794	40.44343195148027	23.09682963140593
19	17.81596275091674	20.42554679615919	38.17581407431832	23.582676378605758
20	22.76735309692955	19.379664878151136	42.709800865281814	15.1431811596375
21	19.26426066364917	26.770780252390512	38.105622333463224	15.85933675049709
22	17.98407321922804	17.53319744611972	43.18690486895876	21.295824465693475
23	21.095740535355656	22.990417953098927	39.55966547765355	16.35417603389187
24	20.053855300100917	21.36326848715566	37.33526173276181	21.247614479981618
25	16.70288660411858	27.62881808099278	36.66681654226991	19.001478772618725
26	16.642436777474696	18.425456870797237	42.97458109768892	21.957525254039147
27	21.982004935903202	21.374259364727276	36.32959643495898	20.31413926441054
28	15.570326628898016	23.880179451055625	40.97049448957365	19.578999430472706
29	20.774507159057983	18.15318285822767	39.61986551162535	21.452444471088995
30	17.32512015027527	27.007583705524414	38.172316976909165	17.49497916729115
31	20.708811686300372	21.33229419581747	35.24999250621983	22.70890161166232
32	27.156460138085386	22.25228060709611	33.68229369623213	16.908965558586374
33	20.923133798946875	21.43570836205949	32.525004246475426	25.11615359251821
34	20.84794620465014	21.67750766863503	35.24224893338529	22.232297193329536
35	23.382342655595856	25.314988559495617	30.58836165982235	20.71430712508618
36	19.009222345453274	31.301020153272784	29.046891080403263	20.64286642087068
37	22.30773458029835	26.14779732821758	33.11426515991727	18.4302029315668
38	19.852022821058522	27.277609584045244	28.375198585174306	24.49516900972193
39	23.48525723649371	23.7320523965109	32.599942048100075	20.182748318895317
40	22.803822827053548	22.46835126844719	30.909095450775858	23.81873045372341
41	18.141692395311892	27.851882937162152	31.482619425876525	22.52380524164943
42	22.58700278768622	22.74487175644215	33.64732272214062	21.020802733731003
43	22.01897425137136	24.785428094681414	28.870787246585333	24.32481040736189
44	21.28932985621934	23.10906947233796	33.24465693474416	22.35694373669854
45	20.032622922973932	22.18808389037099	32.864222695163015	24.91507049149206
46	25.030974291338186	25.16236523685341	30.45647112896296	19.35018934284544
47	17.659842330865384	23.576431561803705	38.78555798687089	19.97816812046002
48	20.815473157279456	23.736548664608375	32.665637520857686	22.78234065725448
49	21.25011240670244	20.141032942657596	37.41219787576311	21.19665677487685
50	20.000649460947415	22.364187724188923	34.58629337649751	23.048869438366157
51	20.06259804362379	20.493989988309703	33.0535655406013	26.3898464274652
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	517.0
2	904.0
3	12313.0
4	23722.0
5	15491.5
6	7261.0
7	6796.0
8	6331.0
9	6146.0
10	5961.0
11	5701.0
12	5441.0
13	5120.0
14	4799.0
15	4664.0
16	4529.0
17	4217.0
18	3905.0
19	3520.5
20	3136.0
21	2999.5
22	2863.0
23	2693.0
24	2523.0
25	2741.0
26	3067.0
27	3175.0
28	3559.0
29	3943.0
30	4171.5
31	4400.0
32	4951.5
33	5503.0
34	6105.5
35	6708.0
36	7109.5
37	7511.0
38	8141.5
39	8772.0
40	9724.5
41	10677.0
42	11575.5
43	12474.0
44	14320.5
45	16167.0
46	36550.0
47	56933.0
48	41634.5
49	26336.0
50	26495.5
51	26655.0
52	24458.0
53	22261.0
54	21499.0
55	20737.0
56	20228.5
57	19720.0
58	18577.5
59	17435.0
60	16486.0
61	15537.0
62	14105.0
63	12673.0
64	11165.5
65	9658.0
66	8311.0
67	6964.0
68	5767.0
69	4570.0
70	3816.0
71	3062.0
72	2460.0
73	1858.0
74	1445.5
75	776.5
76	520.0
77	406.0
78	292.0
79	230.5
80	169.0
81	117.0
82	65.0
83	47.0
84	29.0
85	20.5
86	12.0
87	9.0
88	6.0
89	5.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	2.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	400332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.35016834204606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43126536393055	31.60445689394123
2	7.940564822940088	6.566873843190037
3	3.65584822188585	4.53509818223849
4	2.4356920366850185	4.028651029852261
5	1.861585546180251	3.8488437858836564
6	1.498246907125041	3.717165709654218
7	1.2110837307984579	3.50549613013806
8	1.0150453358697826	3.357783641041936
9	0.7957154339260599	2.9612670430686125
>10	3.068758576011708	18.32389382258176
>50	0.052572241983398965	1.4312160371885172
>100	0.025674815852357636	1.8068407336318475
>500	0.0018339154180255456	0.4870987819961626
>1k	0.003667830836051091	3.112427245832252
>5k	0.001222610278683697	4.743841640748305
>10k+	0.001222610278683697	5.969045479012653
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12812	3.200343714716785	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	10802	2.69826044383162	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	9626	2.4045042614629857	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	9141	2.2833548155031322	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2994	0.7478792602140224	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	2130	0.532058391535026	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	2002	0.5000849295085079	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA	1975	0.4933405273622893	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	1761	0.4398848955367045	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC	1451	0.36244916719123127	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	895	0.22356444151354374	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATG	516	0.12889301879440065	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGT	516	0.12889301879440065	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTAT	407	0.10166561753744392	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0841801304916919	0.0
2	0.0	0.0	0.0	0.9964229689357833	0.0
3	0.0	0.0	0.0	1.1535425596754694	0.0
4	0.0	0.0	0.0	1.8287321523135798	0.0
5	0.0	0.0	0.0	4.682613430852393	0.0
6	0.0	0.0	0.0	5.1524734470389575	0.0
7	0.0	0.0	0.0	5.84290039267408	0.0
8	0.0	0.0	0.0	6.5338269236533675	0.0
9	0.0	0.0	0.0	6.707932416094642	0.0
10	0.0	0.0	0.0	9.88579479032403	0.0
11	0.0	0.0	0.0	10.337419941448598	0.0
12	0.0	0.0	0.0	13.216030694523546	0.0
13	0.0	0.0	0.0	13.56049478932486	0.0
14	0.0	0.0	0.0	13.744841781321503	0.0
15	0.0	0.0	0.0	14.403045472258025	0.0
16	0.0	0.0	0.0	14.753254798517231	0.0
17	0.0	0.0	0.0	15.062997711899124	0.0
18	0.0	0.0	0.0	15.41445600151874	0.0
19	0.0	0.0	0.0	16.184816602220156	0.0
20	0.0	0.0	0.0	16.486815942767503	0.0
21	0.0	0.0	0.0	16.697141372660692	0.0
22	0.0	0.0	0.0	17.009132420091323	0.0
23	0.0	0.0	0.0	17.233196446949034	0.0
24	0.0	0.0	0.0	17.48248953368704	0.0
25	0.0	0.0	0.0	17.64585394122878	0.0
26	0.0	0.0	0.0	17.843190152173698	0.0
27	0.0	0.0	0.0	18.084989458749238	0.0
28	0.0	0.0	0.0	18.257096609813853	0.0
29	0.0	0.0	0.0	18.44069422379425	0.0
30	0.0	0.0	0.0	18.63403375198585	0.0
31	0.0	0.0	0.0	18.835116853012	0.0
32	0.0	0.0	0.0	19.029955137236094	0.0
33	0.0	0.0	0.0	19.218548604658135	0.0
34	0.0	0.0	0.0	19.431122168600062	0.0
35	0.0	0.0	0.0	19.636201952379526	0.0
36	0.0	0.0	0.0	19.823546456441154	0.0
37	0.0	0.0	0.0	20.02313080143481	0.0
38	0.0	0.0	0.0	20.249193169669173	0.0
39	0.0	0.0	0.0	20.489493720212224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGAA	30	2.1619435E-6	45.000004	42
CTTAACG	20	7.027772E-4	45.0	1
CGACCCA	20	7.027772E-4	45.0	34
CTAAGAC	20	7.027772E-4	45.0	18
CGTTAGG	25	3.8862352E-5	45.0	2
TGTTGCG	25	3.8862352E-5	45.0	1
CTACGAA	40	6.7957444E-9	45.0	11
TACGAAT	40	6.7957444E-9	45.0	12
CGAATGG	25	3.8862352E-5	45.0	2
CTCGATG	25	3.8862352E-5	45.0	1
CGATCGA	35	1.2092823E-7	45.0	41
AACAGCG	20	7.027772E-4	45.0	1
ACGACCC	20	7.027772E-4	45.0	36
CGTTTTT	6900	0.0	43.66304	1
GCGATCC	100	0.0	42.75	33
GCGATAC	75	0.0	42.0	33
CCGATGA	1390	0.0	41.92446	18
GATGAAT	1340	0.0	41.809704	20
ATGAATG	1330	0.0	41.785717	21
CGATGAA	1330	0.0	41.785717	19
>>END_MODULE