FastQCFastQC Report
Sat 18 Jun 2016
SRR3552121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428472
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG164443.83782370843369No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC157373.6728187606191303No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC115642.69889280979854No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101282.363748389626393No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC48231.1256278123191248TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT37900.8845385462760693No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA30970.7228010231707089No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT30710.7167329487107675No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT26260.6128755204540787No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC25800.6021396964095671No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC16040.374353516682537No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC10220.23852200377154167TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT9740.22731940476857296Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT8650.20188016953266494No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG8280.19324483280120988No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT7160.16710543512761628No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT5570.1299968259302825No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC5370.1253290763457122No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG4480.10455759069437442No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA4460.1040908157359174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGG253.8866958E-545.02
AACCTAT253.8866958E-545.031
TAATGCG253.8866958E-545.01
TACGGGT302.1623036E-644.9999964
TGCGAAG302.1623036E-644.9999961
CGATGTA302.1623036E-644.99999610
AGGGCGT302.1623036E-644.9999966
CGTTTTT54300.043.466851
CCGATGA8650.042.1387318
GTAGCAT700.041.78571329
CGATGAA8700.041.63792819
GAATGAC3750.041.41
TGATACC18100.041.270724
ACCACCG11200.041.18303314
GATGAAT8800.041.16477220
TACGGCT12900.040.9883737
CGAACCC556.002665E-1140.90909234
GCGAACC556.002665E-1140.90909233
GATACCT18350.040.7084475
CCACCGA10550.040.5213315
Produced by FastQC (version 0.11.5)