##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428472
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.430177467839204	28.0	16.0	31.0	16.0	33.0
2	25.96326481076943	28.0	23.0	31.0	16.0	33.0
3	26.323416699340914	30.0	23.0	33.0	16.0	33.0
4	30.1737663137848	33.0	28.0	35.0	19.0	37.0
5	32.091053791146216	33.0	32.0	35.0	28.0	37.0
6	30.81395517093299	35.0	29.0	35.0	17.0	37.0
7	32.66470621184115	35.0	32.0	35.0	28.0	37.0
8	32.58386545678597	35.0	32.0	35.0	26.0	37.0
9	34.54911406112885	37.0	32.0	39.0	28.0	39.0
10	34.711388375436435	37.0	34.0	39.0	28.0	39.0
11	35.087277581732295	37.0	34.0	39.0	30.0	39.0
12	34.960961276349444	37.0	34.0	39.0	28.0	39.0
13	35.1230652177972	37.0	34.0	39.0	30.0	39.0
14	35.55446096827797	37.0	34.0	40.0	27.0	41.0
15	35.9509302824922	38.0	34.0	40.0	30.0	41.0
16	35.490482458597064	37.0	33.0	40.0	27.0	41.0
17	35.11160122481749	37.0	33.0	39.0	27.0	41.0
18	35.010740491794095	37.0	33.0	39.0	27.0	40.0
19	34.394121436173194	37.0	32.0	39.0	27.0	40.0
20	34.49577568662596	35.0	32.0	39.0	27.0	40.0
21	35.38143916055191	37.0	34.0	39.0	30.0	40.0
22	35.98468978136261	38.0	35.0	39.0	30.0	40.0
23	36.40663800668422	38.0	35.0	40.0	31.0	41.0
24	36.36930767938161	38.0	35.0	40.0	31.0	41.0
25	34.78345376127262	37.0	33.0	39.0	26.0	40.0
26	35.2887539909259	37.0	34.0	39.0	28.0	40.0
27	35.681526447469146	37.0	35.0	40.0	30.0	41.0
28	35.613748389626394	38.0	35.0	40.0	29.0	41.0
29	35.80532216807633	38.0	35.0	40.0	30.0	41.0
30	34.979760637801306	37.0	34.0	40.0	26.0	41.0
31	34.986125114359865	37.0	34.0	40.0	27.0	41.0
32	34.69135439421946	37.0	34.0	40.0	25.0	40.0
33	34.637056797176946	38.0	34.0	40.0	23.0	41.0
34	34.62772596575739	38.0	34.0	40.0	22.0	41.0
35	34.37095306111018	38.0	34.0	40.0	19.0	41.0
36	34.34294656360276	38.0	34.0	40.0	18.0	41.0
37	34.362240706510576	38.0	34.0	40.0	18.0	41.0
38	34.062032058104144	38.0	34.0	40.0	18.0	41.0
39	33.78189006516178	38.0	33.0	40.0	15.0	41.0
40	33.48652187307455	37.0	33.0	40.0	15.0	41.0
41	33.17682835751228	37.0	32.0	40.0	15.0	41.0
42	33.53486108777236	37.0	33.0	40.0	15.0	41.0
43	33.63314989077466	37.0	33.0	40.0	15.0	41.0
44	33.923000802852926	38.0	34.0	40.0	17.0	41.0
45	33.96912750424765	38.0	34.0	40.0	18.0	41.0
46	33.72680595231427	37.0	33.0	40.0	17.0	41.0
47	33.49248025541926	37.0	33.0	40.0	18.0	41.0
48	33.54581629604735	37.0	33.0	40.0	18.0	41.0
49	33.680077111223135	37.0	33.0	40.0	18.0	41.0
50	33.588929965085235	37.0	33.0	40.0	17.0	41.0
51	32.33487369069624	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	23.0
10	21.0
11	19.0
12	21.0
13	20.0
14	43.0
15	59.0
16	99.0
17	215.0
18	437.0
19	936.0
20	1671.0
21	2692.0
22	3735.0
23	4614.0
24	6253.0
25	8688.0
26	10785.0
27	11760.0
28	11543.0
29	12004.0
30	13708.0
31	18378.0
32	24350.0
33	31313.0
34	39763.0
35	57988.0
36	69852.0
37	64812.0
38	30109.0
39	2555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.21498721036614	11.464226367183853	15.653998394294144	25.666788028155867
2	33.10041262906328	33.50930749267163	18.50459306559122	14.885686812673873
3	23.382858156425623	31.427724565432513	31.71479116488359	13.474626113258276
4	21.5001213614892	17.127140163184524	46.67889617057824	14.693842304748033
5	31.17543270038649	19.839802834257547	30.735497302040738	18.24926716331522
6	18.6345898915215	34.52734367706641	33.1652943482888	13.672772083123286
7	56.73066151347113	4.511613360966411	33.86288952370283	4.894835601859632
8	53.83525644616217	15.489926996396497	23.21388562146418	7.460930935977146
9	49.73580537351332	5.381215108571856	22.909081573591738	21.973897944323085
10	35.162157620567974	17.446647622248364	30.80948113295618	16.581713624227486
11	27.74580369312347	18.01377919677365	35.14792098433504	19.092496125767845
12	22.22105528482608	15.081732295225825	39.097070520360724	23.60014189958737
13	21.466280177001064	17.438245672996135	45.08229242517597	16.01318172482683
14	17.11687111409847	26.32237345730876	34.65990776526821	21.90084766332456
15	13.675572732874027	19.376528687988948	47.293872178345374	19.65402640079165
16	15.028053175003267	21.21538863683041	35.37454022666592	28.3820179615004
17	15.963703579230382	22.68619653092851	39.17992307548685	22.170176814354264
18	15.79589798166508	21.550999831961015	38.90662633731026	23.74647584906365
19	16.865279411490132	22.92448514722082	34.08507440392838	26.125161037360666
20	19.956496573871803	22.11883156892399	41.32825482178532	16.596417035418884
21	18.745215556675817	28.016066394070087	35.188063630762336	18.050654418491757
22	16.208527044941093	19.350155902836125	37.86058365540805	26.580733396814725
23	19.139173621613548	25.71953359846151	36.3181258051868	18.82316697473814
24	20.88607890363898	21.417268806363076	32.60936537276648	25.087286917231467
25	16.028818685935136	30.25285199499617	31.75843462349932	21.95989469556937
26	16.923392893818033	19.416671334416254	37.74412330327303	25.91581246849269
27	22.629016598517524	21.78275359883493	31.269021079557124	24.319208723090423
28	14.846944491121942	22.065852611139118	38.305653578296834	24.78154931944211
29	20.23679493642525	17.423775649283968	34.70495154875931	27.63447786553147
30	16.693973001736403	24.071817995108198	34.74486080770739	24.48934819544801
31	19.915653765006816	18.712774697063054	30.407121118766224	30.964450419163914
32	22.089424746541198	21.92115237401744	35.51317238932766	20.476250490113706
33	19.249799286767864	20.01647715603353	32.83225041542971	27.90147314176889
34	24.204382083309994	19.160411882223343	30.81368210758229	25.82152392688437
35	18.47728673052148	19.981469034149256	33.72612446087492	27.815119774454338
36	18.303646445975467	25.834827013200396	28.122257697119064	27.739268843705073
37	18.345889579715827	20.742312216434215	37.66477156033533	23.24702664351463
38	18.069325416830036	22.59237476427865	32.341903321570605	26.996396497320713
39	25.49664855579828	21.3806269721242	33.04113220933924	20.081592262738287
40	19.065656565656568	19.826499747941522	40.18442278608637	20.92342090031554
41	20.67369629754103	28.0300696428238	28.692189921395094	22.604044138240074
42	18.743815231800447	21.602345077391288	38.76962788700312	20.88421180380515
43	21.737476427864596	24.143001176272897	29.278225881737896	24.84129651412461
44	21.82523012005452	20.566104669616685	31.73906346272335	25.869601747605444
45	18.98793853507347	18.89248305606901	33.04393285908998	29.075645549767547
46	25.489646931421422	24.17800929815717	29.896002539255772	20.43634123116563
47	16.767256670214156	21.020276704195375	41.255437928266026	20.957028697324446
48	21.774118262103475	22.0280438395041	29.86216135476764	26.335676543624785
49	18.68966933661943	19.189818704606136	39.19859407382513	22.92191788494931
50	19.989170820963796	19.20498889075599	34.709385910864654	26.09645437741556
51	20.21322280102317	18.927024402994828	30.604566926193545	30.25518586978846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	570.0
2	949.0
3	8770.5
4	16592.0
5	11291.0
6	5990.0
7	5681.0
8	5372.0
9	5082.0
10	4792.0
11	4618.5
12	4445.0
13	4205.0
14	3965.0
15	3723.5
16	3482.0
17	3232.0
18	2982.0
19	2761.5
20	2541.0
21	2349.5
22	2158.0
23	2189.0
24	2220.0
25	2307.5
26	2563.5
27	2732.0
28	3039.5
29	3347.0
30	3788.5
31	4230.0
32	4724.0
33	5218.0
34	5677.5
35	6137.0
36	6567.5
37	6998.0
38	7660.0
39	8322.0
40	9781.0
41	11240.0
42	13210.5
43	15181.0
44	16998.0
45	18815.0
46	50703.5
47	82592.0
48	59923.5
49	37255.0
50	36074.5
51	34894.0
52	29234.0
53	23574.0
54	22006.5
55	20439.0
56	19165.0
57	17891.0
58	17029.5
59	16168.0
60	15455.0
61	14742.0
62	13687.5
63	12633.0
64	11075.0
65	9517.0
66	8117.5
67	6718.0
68	5541.0
69	4364.0
70	3743.0
71	3122.0
72	2580.5
73	2039.0
74	1630.5
75	880.0
76	538.0
77	403.5
78	269.0
79	189.5
80	110.0
81	76.5
82	43.0
83	34.5
84	26.0
85	17.5
86	9.0
87	9.0
88	9.0
89	5.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	428472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.70409379531932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10647066874154	30.97844920531174
2	8.83652789475524	7.193657205061458
3	3.841699376254809	4.691186752334865
4	2.5733018080692847	4.189756726372678
5	1.8589569769633065	3.7833579575888843
6	1.4287167792361257	3.489277307338427
7	1.1510786355379414	3.279752892270719
8	0.8929934217924953	2.90787903993959
9	0.7621406566619379	2.79200202965944
>10	2.4695731417278393	14.274523331753084
>50	0.035488733746894076	0.9673660019585526
>100	0.032579821144689644	2.2799999674068405
>500	0.003490695122645319	1.060195248658125
>1k	0.0046542601635270915	5.354968764330171
>5k	0.0	0.0
>10k+	0.0023271300817635458	12.757627570015448
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	16444	3.83782370843369	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	15737	3.6728187606191303	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	11564	2.69889280979854	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10128	2.363748389626393	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	4823	1.1256278123191248	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	3790	0.8845385462760693	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	3097	0.7228010231707089	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	3071	0.7167329487107675	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	2626	0.6128755204540787	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	2580	0.6021396964095671	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC	1604	0.374353516682537	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1022	0.23852200377154167	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	974	0.22731940476857296	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	865	0.20188016953266494	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG	828	0.19324483280120988	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	716	0.16710543512761628	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT	557	0.1299968259302825	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	537	0.1253290763457122	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	448	0.10455759069437442	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA	446	0.1040908157359174	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23875539125077017	0.0
2	0.0	0.0	0.0	1.7994174648518457	0.0
3	0.0	0.0	0.0	2.124059448458709	0.0
4	0.0	0.0	0.0	3.42939561978379	0.0
5	0.0	0.0	0.0	8.18629922141937	0.0
6	0.0	0.0	0.0	9.023460109412051	0.0
7	0.0	0.0	0.0	10.448057282622901	0.0
8	0.0	0.0	0.0	11.965309285087473	0.0
9	0.0	0.0	0.0	12.392641759554884	0.0
10	0.0	0.0	0.0	17.2800089620792	0.0
11	0.0	0.0	0.0	18.533066338057097	0.0
12	0.0	0.0	0.0	22.470546500121362	0.0
13	0.0	0.0	0.0	23.070352321738643	0.0
14	0.0	0.0	0.0	23.35555182135589	0.0
15	0.0	0.0	0.0	24.376388655501408	0.0
16	0.0	0.0	0.0	24.947954592132042	0.0
17	0.0	0.0	0.0	25.468642058290857	0.0
18	0.0	0.0	0.0	26.051643981403686	0.0
19	0.0	0.0	0.0	27.044707705521013	0.0
20	0.0	0.0	0.0	27.481375679157566	0.0
21	0.0	0.0	0.0	27.88443585578521	0.0
22	0.0	0.0	0.0	28.413291883717022	0.0
23	0.0	0.0	0.0	28.825920946993037	0.0
24	0.0	0.0	0.0	29.15359696782987	0.0
25	0.0	0.0	0.0	29.455366978472338	0.0
26	0.0	0.0	0.0	29.755970051718666	0.0
27	0.0	0.0	0.0	30.103017233331467	0.0
28	0.0	0.0	0.0	30.36627830990123	0.0
29	0.0	0.0	0.0	30.63957504807782	0.0
30	0.0	0.0	0.0	30.937377471573406	0.0
31	0.0	0.0	0.0	31.233546182714388	0.0
32	0.0	0.0	0.0	31.512210832913237	0.0
33	0.0	0.0	0.0	31.763569148042347	0.0
34	0.0	0.0	0.0	32.051335909931105	0.0
35	0.0	0.0	0.0	32.33490169719375	0.0
36	0.0	0.0	0.0	32.61566683470565	0.0
37	0.0	0.0	0.0	32.877760973879276	0.0
38	0.0	0.0	0.0	33.13682107582292	0.0
39	0.0	0.0	0.0	33.39681472768349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGG	25	3.8866958E-5	45.0	2
AACCTAT	25	3.8866958E-5	45.0	31
TAATGCG	25	3.8866958E-5	45.0	1
TACGGGT	30	2.1623036E-6	44.999996	4
TGCGAAG	30	2.1623036E-6	44.999996	1
CGATGTA	30	2.1623036E-6	44.999996	10
AGGGCGT	30	2.1623036E-6	44.999996	6
CGTTTTT	5430	0.0	43.46685	1
CCGATGA	865	0.0	42.13873	18
GTAGCAT	70	0.0	41.785713	29
CGATGAA	870	0.0	41.637928	19
GAATGAC	375	0.0	41.4	1
TGATACC	1810	0.0	41.27072	4
ACCACCG	1120	0.0	41.183033	14
GATGAAT	880	0.0	41.164772	20
TACGGCT	1290	0.0	40.988373	7
CGAACCC	55	6.002665E-11	40.909092	34
GCGAACC	55	6.002665E-11	40.909092	33
GATACCT	1835	0.0	40.708447	5
CCACCGA	1055	0.0	40.52133	15
>>END_MODULE