FastQCFastQC Report
Sat 18 Jun 2016
SRR3552118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713048
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC374205.247893549943342No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337594.734463879009548No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG244583.4300636142307392No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC188242.6399344784642826No Hit
GAACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCT110351.5475816494822228No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC70460.988152270253896No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCT57250.8028912499579272No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTC54230.7605378600038146No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT45870.6432947010579934No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTA39990.5608318093592577No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC21930.30755292771314136No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGT13230.18554150632215505No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATAGGGGT12840.18007202881152462No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTAT10990.15412707138930337No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT9500.13323086243843332Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATG8860.1242553096004757No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG8650.12131020632552085No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC8430.11822486003747294No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG7230.10139569846630242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGG351.211065E-745.0000042
CTCGTAG253.8893104E-545.01
TTGTGCG207.031483E-445.01
TCGGGCA253.8893104E-545.05
GTACGAG207.031483E-445.01
AGGGCGC302.164339E-644.9999966
CGTTTTT181450.044.1939961
TACGGCT24250.041.845367
ACGGCTG24250.041.845368
GAATCTG44250.041.644071
GAATGAC6850.041.3868641
AATCTGT44900.041.1414222
CGGCTGT25000.040.9499979
GCGATTA501.0804797E-940.59
AATGACT6900.040.4347842
GTTTTTT202350.039.7516672
AATGATC7350.039.4897962
TGATACC29100.039.355674
GAATGAT80300.039.0877951
ATGATCT7200.039.0625043
Produced by FastQC (version 0.11.5)