FastQCFastQC Report
Sat 18 Jun 2016
SRR3552112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353894
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC194205.487518861580021No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179655.076378802692331No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC100302.8341819866965814No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG92962.6267752490858847No Hit
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT38661.0924175035462595No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC35340.9986041017931923TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC20680.5843557675462143No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT20620.5826603446229662No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT19690.5563812893126191No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA9080.2565740023848949No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT7710.21786184563739425No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGGATCGTATGC5710.16134774819578743No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT4460.1260264372947832No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTAT4210.11896217511458233No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC4120.11641904072971002No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG4090.11557132926808591TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC650.045.00000417
GGTACTC207.026662E-445.08
GATACCC253.8853148E-545.05
TTGCTAG207.026662E-445.01
TGGTTAC207.026662E-445.029
GCCGATG207.026662E-445.09
GTCCGTC207.026662E-445.044
CGTCCGT207.026662E-445.043
CACCCGT600.044.99999616
CGTTTTT84550.044.1484341
TACGGCT13100.043.4541977
ACGGCTG13500.042.3333328
TGATACC10000.042.34
GAATCTG26200.041.9942741
GATACCT9800.041.7857135
AATCTGT26600.041.701132
CGGCTGT13900.041.115119
GAATGAT33600.040.9821431
AATGATC2800.040.9821432
TCAAGCG1500.040.517