##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552112_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353894
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.38180076520088	28.0	16.0	30.0	16.0	33.0
2	26.7136713253121	30.0	25.0	31.0	16.0	33.0
3	27.342204162828416	30.0	25.0	31.0	16.0	33.0
4	27.598642531379454	30.0	19.0	35.0	19.0	35.0
5	30.026112338722893	32.0	28.0	35.0	19.0	35.0
6	31.03863303701108	33.0	30.0	35.0	17.0	35.0
7	31.98638857963119	33.0	32.0	35.0	27.0	35.0
8	32.912793096237856	35.0	32.0	35.0	28.0	37.0
9	33.84359440962548	35.0	32.0	37.0	27.0	39.0
10	34.209723815605805	35.0	32.0	39.0	27.0	39.0
11	34.75102431801613	37.0	34.0	39.0	27.0	39.0
12	33.055437503885344	35.0	30.0	38.0	25.0	39.0
13	33.91137459239207	35.0	32.0	38.0	27.0	39.0
14	35.2994964593918	37.0	33.0	39.0	27.0	41.0
15	35.07751756175578	37.0	33.0	39.0	27.0	41.0
16	35.68612070280932	37.0	33.0	40.0	27.0	41.0
17	34.25321423929199	36.0	32.0	40.0	25.0	41.0
18	33.96238704244774	36.0	31.0	39.0	25.0	39.0
19	33.69163082730987	36.0	32.0	37.0	25.0	40.0
20	33.507516374959735	35.0	32.0	37.0	25.0	39.0
21	34.440499132508606	35.0	33.0	38.0	27.0	40.0
22	35.172766421583866	36.0	34.0	39.0	30.0	40.0
23	35.452550763788025	36.0	34.0	39.0	30.0	40.0
24	35.21704239122449	36.0	34.0	39.0	30.0	40.0
25	34.31668522212866	35.0	33.0	39.0	26.0	40.0
26	34.387000062165505	35.0	33.0	39.0	25.0	40.0
27	35.12602926299966	36.0	34.0	39.0	30.0	40.0
28	34.901866095497525	36.0	34.0	39.0	27.0	40.0
29	34.903524784257435	36.0	34.0	39.0	27.0	40.0
30	34.536575923864206	36.0	33.0	39.0	25.0	40.0
31	34.349997456865616	36.0	33.0	39.0	25.0	40.0
32	33.6965673337214	35.0	33.0	39.0	21.0	40.0
33	33.21365154537799	36.0	33.0	39.0	18.0	40.0
34	31.835351828513623	35.0	31.0	38.0	14.0	40.0
35	31.266520483534617	35.0	30.0	38.0	10.0	40.0
36	31.26209542970494	35.0	30.0	38.0	10.0	40.0
37	31.041108354479025	35.0	29.0	38.0	9.0	40.0
38	31.172277574640994	35.0	30.0	39.0	9.0	40.0
39	30.62637682469892	35.0	28.0	38.0	8.0	40.0
40	30.183348686329804	35.0	26.0	38.0	8.0	40.0
41	30.10826970787863	35.0	25.0	38.0	8.0	40.0
42	30.03304379277411	35.0	25.0	38.0	8.0	40.0
43	30.29487360622107	35.0	26.0	38.0	8.0	40.0
44	30.21668917811548	35.0	26.0	38.0	8.0	40.0
45	30.415943757170226	35.0	27.0	38.0	8.0	40.0
46	30.04061385612641	35.0	25.0	38.0	8.0	40.0
47	29.87984255172453	35.0	25.0	38.0	8.0	40.0
48	30.141429354552493	35.0	26.0	38.0	8.0	40.0
49	29.790516369308325	34.0	26.0	38.0	8.0	40.0
50	29.763146591917355	34.0	26.0	38.0	8.0	40.0
51	26.71499092948736	31.0	20.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	22.0
11	18.0
12	22.0
13	30.0
14	60.0
15	125.0
16	253.0
17	511.0
18	934.0
19	1501.0
20	2195.0
21	2951.0
22	4210.0
23	5868.0
24	8656.0
25	12651.0
26	15362.0
27	15685.0
28	14966.0
29	14846.0
30	16349.0
31	20631.0
32	25525.0
33	32462.0
34	42036.0
35	50521.0
36	38757.0
37	20784.0
38	5673.0
39	275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32934155425071	11.374027251097786	14.46393552871764	31.83269566593387
2	42.78880116645097	31.130508005221902	14.568204038497404	11.512486789829723
3	19.55331257382154	30.07312924208944	39.95433660926718	10.41922157482184
4	17.880777860037185	14.719944390128118	54.649414796520986	12.749862953313704
5	25.215459996496126	17.107947577523213	40.12670460646408	17.54988781951658
6	15.967210520664379	29.28277958936857	43.814814605503344	10.93519528446371
7	51.835860455390595	3.4360571244496945	41.11400588876895	3.6140765313907552
8	47.07143947057594	11.497510554007699	35.69797736045256	5.733072614963803
9	43.25617275229306	3.859065143800121	33.22096446958694	19.66379763431988
10	28.793084935037044	15.012404844388433	41.887401312257346	14.30710890831718
11	23.113983283129976	15.757260648668812	42.74669816385697	18.38205790434424
12	17.88642926978135	14.049122053496244	49.647352031964374	18.417096644758036
13	16.21135142161212	15.961841681407426	55.33662622141093	12.49018067556952
14	15.879331099142682	23.002933081657222	44.037480149423274	17.080255669776825
15	12.631748489660746	16.936427291787936	55.81106206943321	14.620762149118097
16	14.821952336010218	21.689545457114274	41.60737395943418	21.881128247441325
17	14.98386522518042	18.678191774938256	46.283067811265525	20.0548751886158
18	16.106517770857938	20.366267865519053	44.107840200738075	19.41937416288493
19	14.82449547039509	20.49512000768592	41.83851661797035	22.84186790394864
20	16.332009019649952	22.066494487049795	47.27715078526338	14.324345708036871
21	16.70754519714943	23.787066183659515	45.42913979892284	14.076248820268217
22	14.974822969589766	15.7428495538212	45.53538630211306	23.74694117447597
23	15.625582801629868	22.864473542925282	46.33958190870713	15.170361746737724
24	21.481008437554745	16.70584977422618	41.378774435282885	20.43436735293619
25	15.259088879721046	27.982955348211615	39.32024843597235	17.43770733609499
26	17.16417910447761	16.053111948775623	42.47345250272681	24.30925644401996
27	21.783358858867345	19.434632969194165	39.42112609990562	19.36088207203287
28	15.35742340926944	17.71745211843094	44.54978044273144	22.375344029568176
29	24.58250210515013	18.48406585022634	38.393134667442794	18.540297377180735
30	18.039299903360895	25.14227424030924	41.59833170384352	15.220094152486338
31	21.54430422668935	17.7019107416345	36.52732174040815	24.226463291268008
32	20.545700124896154	27.456526530543044	37.42363532583203	14.574138018728771
33	23.437243920495966	19.608413818827106	34.62166637467716	22.332675885999762
34	29.57326205021843	19.649103968985063	35.425861981271225	15.351771999525281
35	18.04947244090038	24.775780318400425	38.641796696185864	18.53295054451333
36	22.902620558698366	27.0083697378311	31.695931550125177	18.393078153345353
37	17.869192470061655	27.76028980429168	37.62680350613461	16.743714219512057
38	21.405562117470204	22.939072151548203	31.105924372834803	24.54944135814679
39	27.232730704674278	23.906876070235718	31.634331183913826	17.22606204117617
40	23.633912979592758	20.479861201376686	39.596037231487394	16.290188587543163
41	21.534131689149856	29.12708325091694	31.095469264808106	18.243315795125095
42	19.431524693834877	21.219913307374526	41.9526750948024	17.3958869039882
43	25.029811186400448	23.121047545310176	31.39499398124862	20.45414728704075
44	21.228390421990763	20.151514295240947	34.55328431677282	24.066810965995465
45	18.990997304277553	18.65389071303837	33.528966300643695	28.826145682040384
46	27.06347098283667	21.791835973483586	32.58715886677932	18.55753417690043
47	17.531520737848055	19.420221874346556	44.61759735966137	18.43066002814402
48	19.73274483319864	22.23377621547695	33.39785359457917	24.635625356745237
49	17.520500488846942	18.623090529932693	43.97192379639101	19.884485184829355
50	19.722007154684736	18.922332675886	39.91845015739176	21.437210012037504
51	18.98421561258456	17.93446625260671	34.26958354761595	28.811734587192777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	313.5
2	520.0
3	13505.0
4	26490.0
5	17832.0
6	9174.0
7	8339.0
8	7504.0
9	7456.5
10	7409.0
11	7148.0
12	6887.0
13	6441.0
14	5995.0
15	5589.5
16	5184.0
17	4791.5
18	4399.0
19	4051.5
20	3704.0
21	3545.0
22	3386.0
23	3204.5
24	3023.0
25	2886.0
26	2803.0
27	2857.0
28	2984.5
29	3112.0
30	3364.0
31	3616.0
32	3867.0
33	4118.0
34	4468.0
35	4818.0
36	5188.5
37	5559.0
38	6003.5
39	6448.0
40	6932.0
41	7416.0
42	8084.5
43	8753.0
44	10409.5
45	12066.0
46	39044.5
47	66023.0
48	44836.0
49	23649.0
50	23316.0
51	22983.0
52	19815.5
53	16648.0
54	15458.0
55	14268.0
56	13362.0
57	12456.0
58	11771.0
59	11086.0
60	10465.0
61	9844.0
62	9183.0
63	8522.0
64	7759.5
65	6997.0
66	6119.5
67	5242.0
68	4496.0
69	3750.0
70	3205.0
71	2660.0
72	2264.5
73	1869.0
74	1510.0
75	919.0
76	687.0
77	539.5
78	392.0
79	277.0
80	162.0
81	130.5
82	99.0
83	73.0
84	47.0
85	38.0
86	29.0
87	23.5
88	18.0
89	12.0
90	6.0
91	7.0
92	8.0
93	4.0
94	0.0
95	1.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	353894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.35817783827416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.08210423737174	41.92953055323517
2	11.060615856141036	11.582273839933345
3	4.5216973795481366	7.102435065877185
4	2.0131401799463164	4.216174062200179
5	0.9450824592178995	2.474139773578213
6	0.4680531615440859	1.4703846401935026
7	0.2369072133892525	0.8682821006863113
8	0.13063443635413077	0.5471824840345828
9	0.07289248592573432	0.3434865967056144
>10	0.39065546684916824	4.008592404707642
>50	0.044065985190150946	1.5911129300427762
>100	0.02754124074384434	2.968808501334119
>500	0.0016524744446306601	0.6489041313388156
>1k	0.0027541240743844337	3.893136386196743
>5k	5.508248148768867E-4	2.680983468855836
>10k+	0.0016524744446306601	13.674573061079975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	19420	5.487518861580021	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17965	5.076378802692331	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	10030	2.8341819866965814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	9296	2.6267752490858847	No Hit
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	3866	1.0924175035462595	No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	3534	0.9986041017931923	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC	2068	0.5843557675462143	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT	2062	0.5826603446229662	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	1969	0.5563812893126191	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA	908	0.2565740023848949	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT	771	0.21786184563739425	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGGATCGTATGC	571	0.16134774819578743	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	446	0.1260264372947832	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTAT	421	0.11896217511458233	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	412	0.11641904072971002	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	409	0.11557132926808591	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11246305390879754	0.0
2	0.0	0.0	0.0	1.361424607368308	0.0
3	0.0	0.0	0.0	1.6666007335529849	0.0
4	0.0	0.0	0.0	3.0726714779001623	0.0
5	0.0	0.0	0.0	9.932352625362396	0.0
6	0.0	0.0	0.0	11.111519268481523	0.0
7	0.0	0.0	0.0	12.26045086946939	0.0
8	0.0	0.0	0.0	13.52806207508463	0.0
9	0.0	0.0	0.0	14.00814933285108	0.0
10	0.0	0.0	0.0	17.51089309228187	0.0
11	0.0	0.0	0.0	18.16052264237314	0.0
12	0.0	0.0	0.0	21.98257105234901	0.0
13	0.0	0.0	0.0	22.359237511797318	0.0
14	0.0	0.0	0.0	22.651415395570424	0.0
15	0.0	0.0	0.0	23.106636450462567	0.0
16	0.0	0.0	0.0	23.342300236794067	0.0
17	0.0	0.0	0.0	23.58898427212668	0.0
18	0.0	0.0	0.0	23.844710563049954	0.0
19	0.0	0.0	0.0	24.310669296456002	0.0
20	0.0	0.0	0.0	24.53531283378639	0.0
21	0.0	0.0	0.0	24.701181709777504	0.0
22	0.0	0.0	0.0	24.98346962649833	0.0
23	0.0	0.0	0.0	25.159228469541727	0.0
24	0.0	0.0	0.0	25.312946814582897	0.0
25	0.0	0.0	0.0	25.44462466162184	0.0
26	0.0	0.0	0.0	25.598060436175803	0.0
27	0.0	0.0	0.0	25.811683724505077	0.0
28	0.0	0.0	0.0	25.956077243468382	0.0
29	0.0	0.0	0.0	26.105557031201432	0.0
30	0.0	0.0	0.0	26.325114299762074	0.0
31	0.0	0.0	0.0	26.490700605265985	0.0
32	0.0	0.0	0.0	26.664764025386134	0.0
33	0.0	0.0	0.0	26.83345860624933	0.0
34	0.0	0.0	0.0	27.01289086562643	0.0
35	0.0	0.0	0.0	27.203625944491854	0.0
36	0.0	0.0	0.0	27.367799397559722	0.0
37	0.0	0.0	0.0	27.552883066680984	0.0
38	0.0	0.0	0.0	27.767071495984673	0.0
39	0.0	0.0	0.0	28.072812763143766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGTC	65	0.0	45.000004	17
GGTACTC	20	7.026662E-4	45.0	8
GATACCC	25	3.8853148E-5	45.0	5
TTGCTAG	20	7.026662E-4	45.0	1
TGGTTAC	20	7.026662E-4	45.0	29
GCCGATG	20	7.026662E-4	45.0	9
GTCCGTC	20	7.026662E-4	45.0	44
CGTCCGT	20	7.026662E-4	45.0	43
CACCCGT	60	0.0	44.999996	16
CGTTTTT	8455	0.0	44.148434	1
TACGGCT	1310	0.0	43.454197	7
ACGGCTG	1350	0.0	42.333332	8
TGATACC	1000	0.0	42.3	4
GAATCTG	2620	0.0	41.994274	1
GATACCT	980	0.0	41.785713	5
AATCTGT	2660	0.0	41.70113	2
CGGCTGT	1390	0.0	41.11511	9
GAATGAT	3360	0.0	40.982143	1
AATGATC	280	0.0	40.982143	2
TCAAGCG	150	0.0	40.5	17
>>END_MODULE