FastQCFastQC Report
Sat 18 Jun 2016
SRR3552111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739005
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC312284.225681828945677No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG277373.753289896550091No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179772.432595178652377No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC178992.422040446275736No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT91521.2384219321926104No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC88801.2016156859561167TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTACTCCT36280.4909303725955846No Hit
ATGAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT35320.47793993274741037No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT32390.43829202779412857No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC29830.40365085486566393No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT29440.3983734886773432No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTA29220.3953965128788033No Hit
ATGAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC21000.28416587167881135No Hit
GAATGCTGCCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT15890.21501884290363393No Hit
GAATCTTTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC14230.19255620733283266No Hit
GAACTGTCCCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT12240.16562810806422149No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG10350.14005317961312846TruSeq Adapter, Index 20 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT9920.13423454509780042No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGT8660.11718459279707173No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT8100.10960683621897009No Hit
ATGAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT8050.10893025081021102No Hit
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT7920.10717112874743744TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTAT7480.10121717715035758No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC253.8894483E-545.00000432
CGAATAT351.2111377E-745.00000414
TCACGAC207.03165E-445.025
CTCACGT207.03165E-445.045
CACGCGA207.03165E-445.015
TGGTTCG207.03165E-445.01
CCGTAAC207.03165E-445.042
ACGAACG207.03165E-445.01
CGTTTTT87650.043.7421571
GATACCC451.9266736E-840.035
AACGGGA1600.039.3754
CTACGAA403.455989E-739.37511
TACGGCT21450.039.2307667
CGTACTC750.039.043
ACGGCTG21650.038.8683628
TTGATCG356.24526E-638.571431
ACACGAC1400.038.5714326
CACGACC1400.038.5714327
CGTTCGA356.24526E-638.5714314
CCCGTAC700.038.5714341
Produced by FastQC (version 0.11.5)