##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739005
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.954227643926632	26.0	16.0	30.0	16.0	31.0
2	24.41233279883086	27.0	16.0	31.0	10.0	33.0
3	25.497560909601425	28.0	16.0	31.0	16.0	33.0
4	27.918662255329803	32.0	19.0	35.0	19.0	35.0
5	29.66391296405302	32.0	28.0	35.0	19.0	35.0
6	28.898516248198593	33.0	26.0	35.0	15.0	35.0
7	30.113694765258693	32.0	28.0	35.0	21.0	35.0
8	31.5619650746612	35.0	31.0	35.0	25.0	36.0
9	32.41782261283753	34.0	31.0	37.0	25.0	39.0
10	31.256742511890987	34.0	28.0	37.0	17.0	39.0
11	32.21379963599705	35.0	30.0	37.0	17.0	39.0
12	32.31031860406898	35.0	30.0	37.0	22.0	39.0
13	32.69723614860522	35.0	31.0	37.0	25.0	39.0
14	32.65544752741862	36.0	31.0	38.0	20.0	40.0
15	33.10524015399084	36.0	30.0	38.0	23.0	40.0
16	33.259381194985146	36.0	31.0	39.0	23.0	40.0
17	31.32716828708872	34.0	27.0	38.0	17.0	40.0
18	31.987527824574936	34.0	27.0	38.0	18.0	39.0
19	31.89210492486519	34.0	27.0	37.0	18.0	39.0
20	31.719708256371742	34.0	29.0	37.0	21.0	39.0
21	32.92776097590679	35.0	31.0	37.0	24.0	39.0
22	33.861718120987	35.0	32.0	38.0	26.0	39.0
23	34.630539712180564	36.0	33.0	39.0	27.0	40.0
24	32.89407243523386	35.0	31.0	38.0	24.0	39.0
25	32.095680002165075	35.0	30.0	38.0	19.0	39.0
26	32.16615855102469	34.0	30.0	37.0	22.0	39.0
27	33.14497466187644	35.0	31.0	38.0	24.0	40.0
28	33.32209254335221	35.0	31.0	38.0	24.0	40.0
29	33.754910995189476	36.0	32.0	39.0	24.0	40.0
30	32.481448704677234	35.0	30.0	38.0	20.0	40.0
31	32.570450808857856	35.0	30.0	38.0	22.0	40.0
32	32.57661450193165	35.0	30.0	38.0	21.0	40.0
33	33.0534759575375	36.0	31.0	38.0	21.0	40.0
34	32.8235749419828	36.0	31.0	38.0	20.0	40.0
35	32.6877801909324	36.0	31.0	38.0	18.0	40.0
36	32.13265404158294	35.0	31.0	38.0	16.0	40.0
37	32.53607485740962	35.0	31.0	38.0	17.0	40.0
38	32.085071142955734	35.0	30.0	38.0	15.0	40.0
39	31.68648926597249	35.0	30.0	38.0	15.0	40.0
40	31.315678513677174	35.0	30.0	38.0	15.0	40.0
41	30.94558088240269	35.0	28.0	38.0	13.0	40.0
42	32.06386289673277	35.0	30.0	38.0	15.0	40.0
43	32.22914053355525	35.0	31.0	39.0	15.0	40.0
44	32.56804487114431	35.0	31.0	39.0	17.0	40.0
45	32.88641890108998	36.0	32.0	39.0	18.0	40.0
46	32.649220235316406	35.0	32.0	39.0	17.0	40.0
47	32.57385403346392	35.0	31.0	39.0	18.0	40.0
48	32.56045358285803	35.0	32.0	38.0	18.0	40.0
49	32.720874689616444	35.0	32.0	39.0	18.0	40.0
50	32.78245478718006	35.0	32.0	39.0	18.0	40.0
51	30.939107313211682	34.0	28.0	37.0	14.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	23.0
9	52.0
10	69.0
11	57.0
12	61.0
13	79.0
14	137.0
15	216.0
16	484.0
17	850.0
18	1580.0
19	2745.0
20	4561.0
21	6742.0
22	9175.0
23	12246.0
24	16534.0
25	21060.0
26	24604.0
27	27075.0
28	31110.0
29	38357.0
30	49057.0
31	62204.0
32	78167.0
33	96147.0
34	107704.0
35	90047.0
36	44768.0
37	12030.0
38	1056.0
39	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.6774987990609	16.519238706098065	17.459827741354932	20.343434753486108
2	34.1096474313435	30.108862592269336	19.957645753411683	15.823844222975486
3	24.813769866239067	29.636876611119007	31.012510064207955	14.536843458433976
4	20.658588236886082	19.482006211054053	44.73731571504929	15.122089837010574
5	29.01522993755117	22.0393637390816	30.962307426878034	17.9830988964892
6	19.54289889784237	31.375701111629827	34.25646646504422	14.824933525483589
7	60.04127170993431	3.4909100750333217	32.2679819487013	4.199836266331081
8	56.27769771517107	14.024532986921603	22.549779771449447	7.147989526457872
9	51.73036718290134	3.945304835555916	22.8332690577195	21.491058923823246
10	33.4612079755888	18.311107502655595	30.21183889148247	18.015845630273137
11	26.02418116250905	17.956170797220587	35.141304862619336	20.878343177651033
12	21.989431735915183	15.216135208828085	40.836124248144465	21.958308807112264
13	20.49363671423062	18.125723100655613	46.1582803905251	15.22235979458867
14	17.416120323949094	24.239619488366113	36.62654515192725	21.71771503575754
15	14.470808722539088	19.9031129694657	47.15001928268415	18.47605902531106
16	16.578372270823607	22.376573906807128	33.13888268685597	27.90617113551329
17	17.077827619569558	21.99687417541153	38.182150323746114	22.743147881272794
18	17.496092719264418	21.554793269328353	36.48689792355938	24.46221608784785
19	17.523291452696533	24.224869926455167	32.437669569218066	25.81416905163023
20	19.85006867341899	22.55370396682025	40.063734345505104	17.532493014255653
21	20.11718459279707	26.091975020466705	34.86593460125439	18.92490578548183
22	18.170919005960716	18.112056075398677	37.53655252670821	26.180472391932398
23	19.79120574285695	25.3795305850434	36.107198192163786	18.72206547993586
24	22.407696835610043	19.985927023497812	32.808709007381545	24.797667133510597
25	17.631003849770977	29.168002922848967	31.084228117536416	22.11676510984364
26	18.799872801943156	18.526532296804486	37.01991190857978	25.65368299267258
27	23.986982496735475	20.95723303631234	30.85676010311162	24.199024363840568
28	16.604353150519955	20.177130060013127	40.09160966434598	23.12690712512094
29	23.327988308604137	18.99364686301175	36.5368299267258	21.14153490165831
30	22.456952253367703	21.659934641849514	36.30246074113166	19.580652363651126
31	21.787538649941478	19.231128341486187	36.46687099546011	22.514462013112222
32	22.968992090716572	26.57465105107543	30.61751950257441	19.838837355633586
33	19.039789987889122	18.583094836976745	31.013727917943722	31.36338725719041
34	20.02151541599854	20.33085026488319	39.40135723032997	20.246277088788304
35	21.42840711497216	20.111907226608754	28.833769730921983	29.62591592749711
36	19.53626836083653	19.893640773743073	35.47174917625727	25.098341689163135
37	21.26359090939845	24.321756956989464	32.86121203510125	21.553440098510833
38	19.71894642120148	21.637472006278713	29.48369767457595	29.159883897943857
39	22.62677519096623	22.83191588690198	33.696795014918706	20.844513907213077
40	20.770901414740088	20.411905196852526	31.82130026183855	26.99589312656883
41	18.841009194795706	23.859513805725268	31.79274835758892	25.50672864189011
42	19.690123882788342	20.952632255532777	35.45943532181785	23.89780853986103
43	23.883329612113585	19.505280749115364	28.50941468596288	28.101974952808167
44	23.388745678310702	19.557107191426308	31.430504529739313	25.623642600523677
45	20.387954073382453	18.372947409016177	31.51440112042544	29.72469739717593
46	26.01349111305066	23.48103192806544	29.148787897240208	21.356689061643696
47	18.04480348576803	19.208124437588378	39.90825501857227	22.838817058071324
48	21.723263036109365	21.569136879994048	30.575435890149592	26.132164193747
49	18.185803884953415	19.04587925656795	38.68322947747309	24.08508738100554
50	20.29444996989195	18.464286439198652	34.15362548291284	27.087638107996565
51	20.733553900176588	18.348860968464354	31.792883674670673	29.124701456688385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	175.0
1	631.0
2	1087.0
3	14534.0
4	27981.0
5	18918.0
6	9855.0
7	9079.5
8	8304.0
9	7999.0
10	7694.0
11	7377.5
12	7061.0
13	6726.0
14	6391.0
15	5971.5
16	5552.0
17	5111.0
18	4670.0
19	4353.0
20	4036.0
21	3919.5
22	3803.0
23	3650.5
24	3498.0
25	3692.5
26	4289.0
27	4691.0
28	5153.5
29	5616.0
30	6433.0
31	7250.0
32	8135.0
33	9020.0
34	10087.0
35	11154.0
36	12216.5
37	13279.0
38	14025.0
39	14771.0
40	16525.0
41	18279.0
42	21718.0
43	25157.0
44	31220.5
45	37284.0
46	90222.5
47	143161.0
48	102256.5
49	61352.0
50	58036.5
51	54721.0
52	46173.0
53	37625.0
54	35461.0
55	33297.0
56	31635.5
57	29974.0
58	28599.5
59	27225.0
60	26365.5
61	25506.0
62	23840.5
63	22175.0
64	20205.0
65	18235.0
66	16447.5
67	14660.0
68	12622.5
69	10585.0
70	9085.0
71	7585.0
72	6355.0
73	5125.0
74	4321.0
75	2721.5
76	1926.0
77	1442.5
78	959.0
79	731.0
80	503.0
81	369.0
82	235.0
83	156.5
84	78.0
85	54.5
86	31.0
87	25.5
88	20.0
89	13.5
90	7.0
91	4.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	739005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.73590694600356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.07931245089013	29.470011676136103
2	8.21583699942191	6.364957949862806
3	3.7933459810185894	4.40816090804198
4	2.589077812329783	4.0116110885747585
5	1.9614454438033435	3.7989184095414483
6	1.5874272076823182	3.689425956020193
7	1.2484133001066127	3.385089499615808
8	1.0032924273158874	3.109075368331061
9	0.8039179858470595	2.8026443062791278
>10	2.6288683646959567	14.198354785044668
>50	0.04759590167292535	1.2391699887468677
>100	0.028485425147994188	2.2002529718318806
>500	0.00685092503559352	1.839200859079678
>1k	0.0039663250206067755	3.7179288933658827
>5k	7.211500037466864E-4	2.518565453442038
>10k+	0.0014423000074933728	13.246631886085641
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	31228	4.225681828945677	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	27737	3.753289896550091	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17977	2.432595178652377	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	17899	2.422040446275736	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	9152	1.2384219321926104	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	8880	1.2016156859561167	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTACTCCT	3628	0.4909303725955846	No Hit
ATGAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	3532	0.47793993274741037	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	3239	0.43829202779412857	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC	2983	0.40365085486566393	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	2944	0.3983734886773432	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTA	2922	0.3953965128788033	No Hit
ATGAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	2100	0.28416587167881135	No Hit
GAATGCTGCCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	1589	0.21501884290363393	No Hit
GAATCTTTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	1423	0.19255620733283266	No Hit
GAACTGTCCCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	1224	0.16562810806422149	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	1035	0.14005317961312846	TruSeq Adapter, Index 20 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	992	0.13423454509780042	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGT	866	0.11718459279707173	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	810	0.10960683621897009	No Hit
ATGAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	805	0.10893025081021102	No Hit
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	792	0.10717112874743744	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTAT	748	0.10121717715035758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11380166575327637	0.0
2	0.0	0.0	0.0	1.6332771767444063	0.0
3	0.0	0.0	0.0	2.0156832497750354	0.0
4	0.0	0.0	0.0	3.8808939046420523	0.0
5	0.0	0.0	0.0	9.131196676612472	0.0
6	0.0	0.0	0.0	10.275302602824068	0.0
7	0.0	0.0	0.0	11.74579333022104	0.0
8	0.0	0.0	0.0	12.832524813769867	0.0
9	0.0	0.0	0.0	13.240505815251588	0.0
10	0.0	0.0	0.0	17.79825576281622	0.0
11	0.0	0.0	0.0	18.377412872713986	0.0
12	0.0	0.0	0.0	21.806888992631986	0.0
13	0.0	0.0	0.0	22.20472121298232	0.0
14	0.0	0.0	0.0	22.526234599224633	0.0
15	0.0	0.0	0.0	23.12460673473116	0.0
16	0.0	0.0	0.0	23.49280451417785	0.0
17	0.0	0.0	0.0	23.888336344138402	0.0
18	1.3531708175181493E-4	0.0	0.0	24.2646531484902	0.0
19	1.3531708175181493E-4	0.0	0.0	25.02730022124343	0.0
20	1.3531708175181493E-4	0.0	0.0	25.322426776544138	0.0
21	1.3531708175181493E-4	0.0	0.0	25.58142367101711	0.0
22	1.3531708175181493E-4	0.0	0.0	25.99955345363022	0.0
23	1.3531708175181493E-4	0.0	0.0	26.237440883349908	0.0
24	1.3531708175181493E-4	0.0	0.0	26.47830528886814	0.0
25	1.3531708175181493E-4	0.0	0.0	26.6542174951455	0.0
26	1.3531708175181493E-4	0.0	0.0	26.939195269314823	0.0
27	1.3531708175181493E-4	0.0	0.0	27.230397629244727	0.0
28	1.3531708175181493E-4	0.0	0.0	27.41848837287975	0.0
29	1.3531708175181493E-4	0.0	0.0	27.62119336134397	0.0
30	1.3531708175181493E-4	0.0	0.0	27.872341865075338	0.0
31	1.3531708175181493E-4	0.0	0.0	28.095209098720577	0.0
32	1.3531708175181493E-4	0.0	0.0	28.306168429171656	0.0
33	1.3531708175181493E-4	0.0	0.0	28.53241858986069	0.0
34	1.3531708175181493E-4	0.0	0.0	28.75663899432345	0.0
35	1.3531708175181493E-4	0.0	0.0	28.979370910886935	0.0
36	2.7063416350362986E-4	0.0	0.0	29.18302311892342	0.0
37	2.7063416350362986E-4	0.0	0.0	29.379774155790557	0.0
38	2.7063416350362986E-4	0.0	0.0	29.599258462392	0.0
39	2.7063416350362986E-4	0.0	0.0	29.896279456837235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	25	3.8894483E-5	45.000004	32
CGAATAT	35	1.2111377E-7	45.000004	14
TCACGAC	20	7.03165E-4	45.0	25
CTCACGT	20	7.03165E-4	45.0	45
CACGCGA	20	7.03165E-4	45.0	15
TGGTTCG	20	7.03165E-4	45.0	1
CCGTAAC	20	7.03165E-4	45.0	42
ACGAACG	20	7.03165E-4	45.0	1
CGTTTTT	8765	0.0	43.742157	1
GATACCC	45	1.9266736E-8	40.0	35
AACGGGA	160	0.0	39.375	4
CTACGAA	40	3.455989E-7	39.375	11
TACGGCT	2145	0.0	39.230766	7
CGTACTC	75	0.0	39.0	43
ACGGCTG	2165	0.0	38.868362	8
TTGATCG	35	6.24526E-6	38.57143	1
ACACGAC	140	0.0	38.57143	26
CACGACC	140	0.0	38.57143	27
CGTTCGA	35	6.24526E-6	38.57143	14
CCCGTAC	70	0.0	38.57143	41
>>END_MODULE