##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513022
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.116406313959246	28.0	16.0	30.0	16.0	31.0
2	24.662000849866086	28.0	16.0	31.0	16.0	33.0
3	25.706959935441365	30.0	16.0	31.0	16.0	33.0
4	27.308312314091793	30.0	19.0	35.0	19.0	35.0
5	29.66024848836892	32.0	28.0	35.0	19.0	35.0
6	29.068065307140824	33.0	28.0	35.0	15.0	35.0
7	30.27744814062555	32.0	28.0	35.0	22.0	35.0
8	31.752536928240893	35.0	31.0	35.0	25.0	37.0
9	32.66127183629552	34.0	32.0	37.0	25.0	39.0
10	31.702677468022813	34.0	28.0	37.0	17.0	39.0
11	32.6307526772731	35.0	31.0	39.0	22.0	39.0
12	32.77364518480689	35.0	31.0	38.0	23.0	39.0
13	33.171357173766424	35.0	31.0	37.0	25.0	39.0
14	33.129735177048936	36.0	31.0	38.0	22.0	40.0
15	33.48964956668525	36.0	31.0	39.0	25.0	41.0
16	33.70983505580657	36.0	32.0	39.0	25.0	41.0
17	31.847844731804873	34.0	27.0	38.0	18.0	40.0
18	32.39504153817965	34.0	29.0	38.0	18.0	39.0
19	32.236574649820085	35.0	30.0	37.0	18.0	39.0
20	31.841010716889333	34.0	30.0	37.0	22.0	39.0
21	32.997528371102995	35.0	31.0	37.0	25.0	39.0
22	33.742757620530895	35.0	31.0	38.0	26.0	39.0
23	34.51818440534714	36.0	33.0	39.0	27.0	40.0
24	32.82633103453653	35.0	31.0	37.0	24.0	39.0
25	32.02109071345868	35.0	30.0	37.0	19.0	39.0
26	31.765947659164713	34.0	28.0	37.0	21.0	39.0
27	33.037245966059935	35.0	31.0	38.0	24.0	39.0
28	33.20831075470448	35.0	31.0	38.0	24.0	40.0
29	33.355733282393345	35.0	31.0	38.0	23.0	40.0
30	32.14633875350375	35.0	30.0	38.0	19.0	40.0
31	32.42769121012354	35.0	30.0	38.0	21.0	40.0
32	32.46187492934026	35.0	31.0	38.0	21.0	40.0
33	32.61925609428056	35.0	31.0	38.0	18.0	40.0
34	31.948863791416354	35.0	31.0	38.0	15.0	40.0
35	31.722569402481763	35.0	30.0	38.0	13.0	40.0
36	31.31387152987591	35.0	30.0	38.0	10.0	40.0
37	31.605437973420244	35.0	30.0	38.0	10.0	40.0
38	31.327943051175193	35.0	30.0	38.0	10.0	40.0
39	30.694159314805212	35.0	29.0	38.0	10.0	40.0
40	30.251542818826483	34.0	26.0	38.0	10.0	40.0
41	29.90109001173439	34.0	25.0	38.0	9.0	40.0
42	30.918861179442597	35.0	29.0	38.0	10.0	40.0
43	31.08282490809361	35.0	30.0	38.0	10.0	40.0
44	31.38911781561025	35.0	30.0	38.0	10.0	40.0
45	31.762593027199614	35.0	31.0	39.0	10.0	40.0
46	31.46700141514399	35.0	30.0	39.0	10.0	40.0
47	31.33243213741321	35.0	30.0	38.0	10.0	40.0
48	31.387201718444825	35.0	30.0	38.0	10.0	40.0
49	31.55277161603206	35.0	31.0	38.0	10.0	40.0
50	31.579148652494435	35.0	30.0	39.0	10.0	40.0
51	29.970819964835815	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	28.0
10	48.0
11	48.0
12	47.0
13	80.0
14	92.0
15	174.0
16	311.0
17	699.0
18	1270.0
19	2182.0
20	3368.0
21	5031.0
22	6984.0
23	10139.0
24	13621.0
25	17877.0
26	20836.0
27	21994.0
28	23643.0
29	27571.0
30	33209.0
31	41796.0
32	52288.0
33	63863.0
34	70973.0
35	58561.0
36	28263.0
37	7436.0
38	569.0
39	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.611926973891954	13.139592454124774	16.425221530460682	25.823259041522583
2	38.433829348448995	30.081361033250037	16.82949269232119	14.655316925979783
3	21.57158952247662	29.29913337049873	35.663967627119305	13.465309479905347
4	19.542436776590478	16.74626039429108	49.503530063038234	14.207772766080208
5	28.748864571110012	18.879307320153913	35.631025570053524	16.74080253868255
6	18.18518504079747	31.031612679378274	36.899002381964124	13.88419989786013
7	55.124536569581814	3.448974897762669	38.1615213382662	3.2649671943893246
8	52.723469948657176	13.761787993497354	27.019504036863918	6.495238020981556
9	48.02211211215114	3.9206895610714545	26.62536889256211	21.431829434215295
10	31.760821173360988	16.140828268573276	34.460705388852716	17.637645169213016
11	24.95058691440133	17.14253969615338	39.42793876286007	18.478934626585215
12	19.514172881474867	14.763304497662869	44.37451025492084	21.348012365941422
13	18.59140543680388	17.34272604293773	50.07407089754435	13.991797622714037
14	16.689732604059866	23.009734475324645	40.04272721247822	20.257805708137273
15	13.368627466268505	18.528835020720358	50.329225647243206	17.77331186576794
16	15.228001918046399	20.11941008377808	37.58708203546826	27.065505962707253
17	15.838891899372737	20.653305316341207	42.58472346215172	20.923079322134335
18	16.488181793373382	19.33289410590579	41.12806078491761	23.050863315803223
19	16.089368487121412	22.48480571983268	37.47188229744533	23.95394349560058
20	18.657289550935438	20.425049997855844	45.170577480108065	15.747082971100653
21	18.799778567001024	24.981384813906615	39.240422438024105	16.978414181068256
22	16.477461005570913	16.570049627501355	42.153552869077735	24.798936497849994
23	18.465289987563885	23.9026786375633	39.99614051639111	17.635890858481705
24	20.82971100654553	18.987294891837	37.15844544678396	23.024548654833517
25	16.27162187976344	28.013223604445812	35.32421611548823	20.39093840030252
26	17.114470724452364	19.020626795731957	40.02245517736082	23.842447302454868
27	20.6636362573145	19.736385574107935	36.83896597027028	22.761012198307284
28	15.062706862473735	21.20396396255911	42.76132407577063	20.972005099196526
29	19.566217433170507	21.560283964430376	37.43816054672119	21.435338055677924
30	17.296529193679802	21.649753811727372	44.37665441248134	16.67706258211149
31	19.48571406294467	17.73023379114346	40.01114961931457	22.7729025265973
32	21.683085715622333	21.83142243412563	34.45232368202533	22.033168168226705
33	21.369453941546364	17.645442105796633	34.40359282837773	26.581511124279274
34	20.210634241806392	23.430574127425334	37.23446557847422	19.124326052294055
35	17.184643153704908	19.884527369196643	35.21603362039055	27.714795856707898
36	18.817126750899572	23.332722573300952	34.43575519178515	23.41439548401433
37	17.267680528320422	20.46169559979884	38.03326173146571	24.23736214041503
38	23.574427607393055	21.516426196147535	32.12103964352406	22.788106552935353
39	21.965919590193014	20.315308115441443	34.7495818892757	22.96919040508984
40	23.08809368798999	20.38080238274382	33.77165111827563	22.759452810990563
41	17.96492158231031	24.092339119959767	33.68315588805159	24.25958340967834
42	18.84675510991731	21.008455777724933	36.47036579327982	23.674423319077935
43	23.039167910927798	20.007134196974008	29.869674205004852	27.08402368709334
44	22.231210357450557	19.56368342878083	33.775939433396616	24.429166780371993
45	19.388057432234877	18.779701455298216	33.73695475047854	28.095286361988375
46	24.90322052465586	22.824557231463757	31.204509748119964	21.067712495760414
47	18.098054274475558	19.183387846914947	42.51766980753262	20.200888071076875
48	20.832829781178972	21.78308142730721	32.63758669218864	24.746502099325173
49	17.39594013512091	18.852212965525844	40.02927749687148	23.722569402481767
50	20.211024088635575	18.49725742755671	35.8327713041546	25.458947179653112
51	20.219015948633782	18.836814015773204	33.09097855452593	27.853191481067093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	125.0
1	438.0
2	751.0
3	14316.5
4	27882.0
5	19131.0
6	10380.0
7	9545.0
8	8710.0
9	8415.0
10	8120.0
11	7817.0
12	7514.0
13	7084.0
14	6654.0
15	6059.0
16	5464.0
17	5111.0
18	4758.0
19	4442.0
20	4126.0
21	3916.5
22	3707.0
23	3507.0
24	3307.0
25	3306.5
26	3376.5
27	3447.0
28	3649.0
29	3851.0
30	4414.5
31	4978.0
32	5366.0
33	5754.0
34	6507.0
35	7260.0
36	8084.0
37	8908.0
38	9655.0
39	10402.0
40	11402.5
41	12403.0
42	13735.0
43	15067.0
44	17780.0
45	20493.0
46	57685.5
47	94878.0
48	67703.0
49	40528.0
50	38467.0
51	36406.0
52	30955.0
53	25504.0
54	23606.0
55	21708.0
56	20514.5
57	19321.0
58	18357.0
59	17393.0
60	16542.5
61	15692.0
62	14670.0
63	13648.0
64	12684.0
65	11720.0
66	10439.0
67	9158.0
68	7837.0
69	6516.0
70	5703.0
71	4890.0
72	4146.5
73	3403.0
74	2831.5
75	1809.0
76	1358.0
77	1046.0
78	734.0
79	511.5
80	289.0
81	214.0
82	139.0
83	100.5
84	62.0
85	44.5
86	27.0
87	19.5
88	12.0
89	8.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	513022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.24776066069204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07013606238658	35.64321206700225
2	9.341645831450176	8.640604011797185
3	4.890546666728111	6.785304952283607
4	3.057570592751758	5.656231719070144
5	1.9193123036235216	4.438194802555106
6	1.276269994833835	3.541477755569872
7	0.7993847779135717	2.5878829119323123
8	0.5133954696434372	1.8994712643482614
9	0.3081504110404242	1.282613981156227
>10	0.7445915024236458	5.500168695213324
>50	0.04446244744454802	1.386297886529475
>100	0.027195477561314418	2.7719968538942754
>500	0.001295022741014972	0.40387105764696574
>1k	0.0034533939760399263	2.8881871433409065
>5k	8.633484940099816E-4	2.452373738079747
>10k+	0.0017266969880199632	14.122111159580358
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	19699	3.8397963440164355	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	19268	3.7557843523279706	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17326	3.377243081193399	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	14445	2.8156687237584355	No Hit
GAACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT	6366	1.2408824572825337	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	5918	1.1535567675460312	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTCGTCCT	3076	0.5995844232800932	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTA	2247	0.4379929125846455	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTC	2017	0.39316052722885175	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTT	1998	0.389456982351634	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCT	1890	0.36840525357587006	No Hit
GAATGCTGCCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTT	1122	0.21870407117043716	No Hit
GAACTGTCCCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT	1064	0.2073985131241935	TruSeq Adapter, Index 22 (95% over 21bp)
GAATCTTTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	1053	0.20525435556369903	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGT	725	0.1413194755780454	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATG	699	0.1362514667986948	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTAT	599	0.11675912533965405	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08537645559059845	0.0
2	0.0	0.0	0.0	1.447696200162956	0.0
3	0.0	0.0	0.0	1.7383270113172535	0.0
4	0.0	0.0	0.0	3.398879580212934	0.0
5	0.0	0.0	0.0	8.139222099637053	0.0
6	0.0	0.0	0.0	9.053217990651474	0.0
7	0.0	0.0	0.0	9.910881014849267	0.0
8	0.0	0.0	0.0	10.819613973669744	0.0
9	0.0	0.0	0.0	11.197765397975136	0.0
10	0.0	0.0	0.0	15.686266865748447	0.0
11	0.0	0.0	0.0	16.235950894893396	0.0
12	0.0	0.0	0.0	19.798955990191455	0.0
13	0.0	0.0	0.0	20.209659624733444	0.0
14	0.0	0.0	0.0	20.466373761749008	0.0
15	0.0	0.0	0.0	21.096366237705205	0.0
16	0.0	0.0	0.0	21.474517662010594	0.0
17	0.0	0.0	0.0	21.84545691997614	0.0
18	0.0	0.0	0.0	22.20294646233495	0.0
19	0.0	0.0	0.0	23.09861955237787	0.0
20	0.0	0.0	0.0	23.398801610847098	0.0
21	0.0	0.0	0.0	23.616141218115402	0.0
22	0.0	0.0	0.0	23.995072336079154	0.0
23	0.0	0.0	0.0	24.226446429197967	0.0
24	0.0	0.0	0.0	24.445540347197586	0.0
25	0.0	0.0	0.0	24.61531864130583	0.0
26	0.0	0.0	0.0	24.86930385051713	0.0
27	0.0	0.0	0.0	25.133620000701725	0.0
28	1.9492341459040742E-4	0.0	0.0	25.30846630358932	0.0
29	1.9492341459040742E-4	0.0	0.0	25.482922759647735	0.0
30	1.9492341459040742E-4	0.0	0.0	25.684863417163395	0.0
31	1.9492341459040742E-4	0.0	0.0	25.88310052980184	0.0
32	1.9492341459040742E-4	0.0	0.0	26.097516285851288	0.0
33	1.9492341459040742E-4	0.0	0.0	26.295948321904323	0.0
34	1.9492341459040742E-4	0.0	0.0	26.511728541855906	0.0
35	1.9492341459040742E-4	0.0	0.0	26.721271212540593	0.0
36	1.9492341459040742E-4	0.0	0.0	26.91580478030182	0.0
37	1.9492341459040742E-4	0.0	0.0	27.105075415869106	0.0
38	1.9492341459040742E-4	0.0	0.0	27.309939924603622	0.0
39	1.9492341459040742E-4	0.0	0.0	27.546187103087195	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	30	2.1631422E-6	45.000004	1
ACGTCGG	25	3.8877748E-5	45.000004	2
GCATATT	30	2.1631422E-6	45.000004	27
GCTTGCG	35	1.2101737E-7	45.0	1
TACGCAG	20	7.0296315E-4	45.0	1
TTCGAAT	20	7.0296315E-4	45.0	16
CGTTTTT	8945	0.0	44.019005	1
TACGGCT	1730	0.0	43.049133	7
ACGGCTG	1725	0.0	43.04348	8
CCATTCG	80	0.0	42.1875	41
GATACCT	2285	0.0	42.04595	5
TCAAGCG	140	0.0	41.785713	17
TGATACC	2305	0.0	41.583515	4
ATACCTG	2340	0.0	41.346157	6
CGGCTGT	1780	0.0	40.82865	9
TACCTGT	2410	0.0	40.518673	7
AATTCGA	50	1.0786607E-9	40.500004	13
AAGTGCG	50	1.0786607E-9	40.500004	12
CACCGGT	195	0.0	40.384617	16
GACACGA	145	0.0	40.34483	25
>>END_MODULE