SRR3552107_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387723
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	17648	4.551703148897538	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	15460	3.987382744897775	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12870	3.319380072887087	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	12864	3.31783257634961	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	4365	1.1258037310141518	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	3333	0.8596343265681943	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATT	3039	0.7838069962318459	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTA	2606	0.6721293294439588	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	1713	0.44181026144954	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTC	1614	0.4162765685811778	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATG	765	0.1973058085282534	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	750	0.1934370671845622	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGT	730	0.18827874539297385	No Hit
GAACTGTCCCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	704	0.181572927063909	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT	613	0.15810256291218214	No Hit
GAATCTTTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	606	0.15629715028512625	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	503	0.12973179305844637	No Hit
GAATGCTGCCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	502	0.12947387696886695	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGCTAT	463	0.11941514947526972	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCGCAGCT	458	0.11812556902737265	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
GAATGACTCTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	416	0.10729309326503716	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	30	2.161767E-6	45.000004	5
GCGTATG	30	2.161767E-6	45.000004	1
ATTCGGT	20	7.027498E-4	45.0	28
TACGGGT	20	7.027498E-4	45.0	4
TACGGAT	20	7.027498E-4	45.0	29
TCGATCA	20	7.027498E-4	45.0	17
ACGGATC	20	7.027498E-4	45.0	30
GCTTACG	20	7.027498E-4	45.0	1
ATAACGG	20	7.027498E-4	45.0	2
ATACGGA	20	7.027498E-4	45.0	28
CACCCGT	40	6.7957444E-9	45.0	16
CTTACGG	35	1.2091368E-7	45.0	2
ACCGGTC	55	1.8189894E-12	44.999996	17
CGTTTTT	6170	0.0	44.2342	1
TACGGCT	1915	0.0	42.18016	7
CACCGGT	215	0.0	41.860466	16
ACCGGTG	140	0.0	41.785713	17
ACGGCTG	1955	0.0	41.547318	8
ATGACTC	65	0.0	41.538464	3
GACGGGT	60	3.6379788E-12	41.250004	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content