##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387723
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.380157999396477	28.0	16.0	30.0	16.0	31.0
2	24.72437023338827	28.0	16.0	31.0	16.0	33.0
3	25.55139622875094	28.0	16.0	31.0	16.0	33.0
4	27.61319808213596	32.0	19.0	35.0	19.0	35.0
5	29.819332358410513	32.0	28.0	35.0	19.0	35.0
6	28.87184149508799	33.0	26.0	35.0	15.0	35.0
7	30.173701328009944	32.0	28.0	35.0	23.0	35.0
8	31.436546194061226	35.0	31.0	35.0	23.0	36.0
9	32.586400600428654	34.0	32.0	37.0	25.0	39.0
10	31.535944475824234	34.0	28.0	37.0	17.0	39.0
11	32.482803444727296	35.0	31.0	39.0	22.0	39.0
12	32.629547383054394	35.0	30.0	37.0	23.0	39.0
13	33.04563825205108	35.0	31.0	37.0	25.0	39.0
14	32.969818659197415	36.0	31.0	38.0	21.0	40.0
15	33.22765479478906	36.0	30.0	39.0	23.0	40.0
16	33.583901393520634	36.0	32.0	39.0	25.0	41.0
17	31.584401750734415	34.0	27.0	38.0	18.0	40.0
18	32.16164375082211	34.0	27.0	38.0	18.0	39.0
19	32.1435560954599	34.0	29.0	37.0	18.0	39.0
20	31.673663930176957	34.0	29.0	37.0	21.0	39.0
21	33.01350190728948	35.0	31.0	37.0	25.0	39.0
22	33.80360721442886	35.0	32.0	38.0	26.0	39.0
23	34.375216327120135	36.0	33.0	39.0	27.0	40.0
24	32.911168540427056	35.0	31.0	38.0	24.0	39.0
25	32.03843465566912	35.0	30.0	37.0	19.0	39.0
26	32.04664154563954	34.0	30.0	37.0	22.0	39.0
27	33.11396280334156	35.0	31.0	38.0	24.0	39.0
28	33.26685030292245	35.0	31.0	38.0	24.0	40.0
29	33.21841624046033	35.0	31.0	38.0	23.0	40.0
30	32.11242304428677	35.0	30.0	38.0	19.0	40.0
31	32.44547782824336	35.0	30.0	38.0	22.0	40.0
32	32.3621735104701	35.0	30.0	38.0	21.0	40.0
33	32.69897323604739	35.0	31.0	38.0	20.0	40.0
34	32.315792460081035	35.0	31.0	38.0	16.0	40.0
35	31.984094314755637	35.0	31.0	38.0	15.0	40.0
36	31.692571758704023	35.0	30.0	38.0	13.0	40.0
37	31.99118700721907	35.0	31.0	38.0	12.0	40.0
38	31.42746754770803	35.0	30.0	38.0	11.0	40.0
39	31.10820611622215	35.0	30.0	38.0	10.0	40.0
40	30.634940408487502	34.0	28.0	38.0	10.0	40.0
41	30.205551385912106	34.0	26.0	38.0	10.0	40.0
42	31.275947003401914	35.0	30.0	38.0	10.0	40.0
43	31.352150891229048	35.0	30.0	38.0	10.0	40.0
44	31.858553658152857	35.0	31.0	38.0	12.0	40.0
45	32.04570788939527	35.0	31.0	39.0	11.0	40.0
46	31.804061146746516	35.0	31.0	39.0	10.0	40.0
47	31.698359395754185	35.0	31.0	38.0	11.0	40.0
48	31.67493029817679	35.0	31.0	38.0	12.0	40.0
49	31.927241871129645	35.0	31.0	38.0	12.0	40.0
50	31.791668278642227	35.0	31.0	39.0	10.0	40.0
51	30.296224366364648	34.0	28.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	23.0
10	34.0
11	30.0
12	32.0
13	57.0
14	59.0
15	125.0
16	267.0
17	510.0
18	917.0
19	1644.0
20	2500.0
21	3618.0
22	5108.0
23	7103.0
24	9746.0
25	12475.0
26	14816.0
27	15722.0
28	17698.0
29	21089.0
30	26153.0
31	32903.0
32	40299.0
33	48843.0
34	53845.0
35	44065.0
36	21667.0
37	5875.0
38	482.0
39	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36202391913815	13.550395514323371	17.912272421290456	23.175308145248025
2	36.998578882346415	32.374401312277065	16.44008738197115	14.186932423405368
3	21.134160212316523	31.970762632085275	33.5780957023442	13.316981453253998
4	19.55648749235924	17.324481653138967	50.336709454945925	12.782321399555869
5	31.4843328871385	19.352475865501916	33.61858852840817	15.544602718951417
6	17.807558488921217	35.44669777134707	33.85845049171702	12.887293248014691
7	54.47007270654565	3.7170866830185467	38.532921699254366	3.2799189111814364
8	52.194221132096885	17.136718739925154	24.757881270907323	5.911178857070641
9	47.70854450213168	4.394632250343673	24.120054781377426	23.776768466147225
10	34.06658877600761	16.212347474872523	31.949355596650186	17.771708152469675
11	27.240839465288364	17.105252976996464	37.71764894009383	17.93625861762134
12	21.050853315382373	14.155724576566259	41.78421192449249	23.00921018355888
13	19.389357866311773	16.26264111234051	49.96531028595157	14.382690735396148
14	17.58858772886829	23.674891610763353	37.79811876004261	20.938401900325747
15	12.760914364120776	18.081723292144133	50.744990624750145	18.412371718984947
16	14.608109397688557	19.18379874291698	36.74375778584195	29.46433407355251
17	15.846364543759334	20.09269504259484	43.31984432184833	20.741096091797495
18	15.231750502291586	19.321783850841967	41.025164872860266	24.421300774006184
19	16.36400213554522	22.189810766965078	37.34109144930788	24.10509564818182
20	19.037818236215028	19.472406847156346	45.91834892436095	15.571425992267676
21	19.311983039437948	26.619519605491547	37.457669521797776	16.610827833272722
22	16.21492663576832	17.42635850852284	41.65267471880698	24.706040136901862
23	18.531528952370635	25.665487990137287	38.34851169520508	17.454471362286995
24	20.461257134603827	18.743020145825756	35.34585258032152	25.449870139248898
25	15.590511782896552	29.583749223027777	33.6557284453076	21.170010548768065
26	17.485937125215685	18.231056708010616	39.58418768038007	24.698818486393638
27	19.955741599028173	21.784366674146234	34.11198200777359	24.147909719052006
28	16.056824072856138	21.387175896193934	42.22602218594203	20.329977845007903
29	26.229292561957894	16.39443623411559	34.605375487139014	22.7708957167875
30	16.680465177459165	24.102516487286028	36.95112232186381	22.265896013391004
31	22.342755008085668	21.440822442826452	32.720008872313485	23.496413676774395
32	26.40415967069274	25.135212509962006	30.619282322689134	17.841345496656118
33	18.015438857122223	18.7425043136466	32.32539725525697	30.916659573974204
34	27.23000698952603	20.95078187262556	34.562303500179254	17.256907637669162
35	18.429136264807607	23.39892139491338	30.822262285188135	27.349680055090875
36	23.165765249933585	25.490620881402442	32.59672498149452	18.74688888716945
37	23.21296389432662	21.140350198466432	33.861545484791975	21.785140422414973
38	19.536627953461622	26.862476561875358	27.904199647686625	25.696695836976396
39	26.497783211210063	20.455067148453924	35.06266071396332	17.984488926372695
40	18.927688065964617	25.619063094012994	31.72677401134315	23.726474828679237
41	17.940127358965036	24.27712567993129	38.09781725613389	19.684929704969786
42	19.168323777542216	25.613646856131826	35.95195539083315	19.266073975492816
43	23.071110045057942	19.751214139991696	33.09630844700985	24.081367367940516
44	22.330375035785856	19.998813585987936	33.439594762240056	24.231216615986156
45	18.57769593240535	20.8538054229437	32.917830513020895	27.650668131630056
46	27.032701180997776	24.059702416415842	30.64997433734909	18.257622065237296
47	17.22518395865089	19.388326201953458	43.180827549565024	20.205662289830624
48	21.679136909597833	22.96097987480753	31.69143950707077	23.668443708523867
49	17.714450780583046	20.03492183852905	39.532862378553766	22.71776500233414
50	21.327597279501088	18.370331396383502	35.36751753184619	24.934553792269224
51	21.208955878294557	18.447964139346908	31.923563987692244	28.419515994666295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	353.0
2	605.0
3	10591.5
4	20578.0
5	13929.5
6	7281.0
7	6667.0
8	6053.0
9	5831.5
10	5610.0
11	5249.5
12	4889.0
13	4583.0
14	4277.0
15	3934.5
16	3592.0
17	3310.5
18	3029.0
19	2829.0
20	2629.0
21	2614.0
22	2599.0
23	2477.0
24	2355.0
25	2331.0
26	2433.5
27	2560.0
28	2801.5
29	3043.0
30	3524.0
31	4005.0
32	4220.0
33	4435.0
34	5011.0
35	5587.0
36	6047.0
37	6507.0
38	7140.5
39	7774.0
40	8708.5
41	9643.0
42	10513.5
43	11384.0
44	14125.5
45	16867.0
46	46665.5
47	76464.0
48	53920.0
49	31376.0
50	29523.5
51	27671.0
52	23343.5
53	19016.0
54	17467.0
55	15918.0
56	15132.0
57	14346.0
58	14019.5
59	13693.0
60	13078.0
61	12463.0
62	11351.5
63	10240.0
64	9294.5
65	8349.0
66	7359.0
67	6369.0
68	5516.5
69	4664.0
70	4132.5
71	3601.0
72	3009.0
73	2417.0
74	2008.0
75	1233.0
76	867.0
77	682.0
78	497.0
79	364.5
80	232.0
81	177.0
82	122.0
83	91.0
84	60.0
85	40.5
86	21.0
87	15.0
88	9.0
89	10.0
90	11.0
91	8.5
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	387723.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.850361912446225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86524483477002	32.93703559658683
2	8.539610065387482	7.318507637860443
3	4.400307278678521	5.65664278252038
4	2.942965748691317	5.044285897094248
5	2.0720535181274027	4.439412157685832
6	1.5484195822468276	3.981020369495721
7	1.040733826636166	3.1217074788119343
8	0.7475237496690035	2.562533056917252
9	0.47248602072472273	1.8221577287963344
>10	1.285735578229515	8.135769357075459
>50	0.04553670265283171	1.3495365025899044
>100	0.028306598946354847	2.3560196660103156
>500	0.004922886773279104	1.3640391418322735
>1k	0.003692165079959328	4.395618112187124
>5k	0.0	0.0
>10k+	0.002461443386639552	15.515714514535981
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	17648	4.551703148897538	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	15460	3.987382744897775	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12870	3.319380072887087	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	12864	3.31783257634961	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	4365	1.1258037310141518	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	3333	0.8596343265681943	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATT	3039	0.7838069962318459	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTA	2606	0.6721293294439588	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	1713	0.44181026144954	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTC	1614	0.4162765685811778	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATG	765	0.1973058085282534	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	750	0.1934370671845622	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGT	730	0.18827874539297385	No Hit
GAACTGTCCCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	704	0.181572927063909	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT	613	0.15810256291218214	No Hit
GAATCTTTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	606	0.15629715028512625	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	503	0.12973179305844637	No Hit
GAATGCTGCCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	502	0.12947387696886695	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGCTAT	463	0.11941514947526972	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCGCAGCT	458	0.11812556902737265	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
GAATGACTCTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	416	0.10729309326503716	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1413380170895201	0.0
2	0.0	0.0	0.0	1.5263474181309853	0.0
3	0.0	0.0	0.0	1.7811685146354486	0.0
4	0.0	0.0	0.0	2.961392540550857	0.0
5	0.0	0.0	0.0	7.0625162809531545	0.0
6	0.0	0.0	0.0	7.672487832808474	0.0
7	0.0	0.0	0.0	8.507620130866623	0.0
8	0.0	0.0	0.0	9.374218191853462	0.0
9	0.0	0.0	0.0	9.629555120537084	0.0
10	0.0	0.0	0.0	14.978734818414177	0.0
11	2.579160895794162E-4	0.0	0.0	15.67949283380145	0.0
12	2.579160895794162E-4	0.0	0.0	20.569323976137603	0.0
13	2.579160895794162E-4	0.0	0.0	21.084640323117277	0.0
14	2.579160895794162E-4	0.0	0.0	21.356483881533983	0.0
15	2.579160895794162E-4	0.0	0.0	22.288334713184412	0.0
16	2.579160895794162E-4	0.0	0.0	22.79178692004343	0.0
17	2.579160895794162E-4	0.0	0.0	23.22534386662643	0.0
18	2.579160895794162E-4	0.0	0.0	23.662769554553122	0.0
19	2.579160895794162E-4	0.0	0.0	24.819007384137645	0.0
20	2.579160895794162E-4	0.0	0.0	25.217745658627422	0.0
21	2.579160895794162E-4	0.0	0.0	25.495521287104452	0.0
22	2.579160895794162E-4	0.0	0.0	25.972666052826373	0.0
23	2.579160895794162E-4	0.0	0.0	26.237803792914015	0.0
24	2.579160895794162E-4	0.0	0.0	26.50809985479324	0.0
25	2.579160895794162E-4	0.0	0.0	26.678324473915655	0.0
26	2.579160895794162E-4	0.0	0.0	26.909933122357973	0.0
27	2.579160895794162E-4	0.0	0.0	27.2065366253743	0.0
28	2.579160895794162E-4	0.0	0.0	27.40152118909634	0.0
29	2.579160895794162E-4	0.0	0.0	27.60063241025165	0.0
30	2.579160895794162E-4	0.0	0.0	27.824245659917004	0.0
31	2.579160895794162E-4	0.0	0.0	28.03702643382002	0.0
32	2.579160895794162E-4	0.0	0.0	28.24980720772304	0.0
33	2.579160895794162E-4	0.0	0.0	28.466972555148907	0.0
34	2.579160895794162E-4	0.0	0.0	28.701160364487016	0.0
35	2.579160895794162E-4	0.0	0.0	28.91600446710667	0.0
36	2.579160895794162E-4	0.0	0.0	29.13316981453254	0.0
37	2.579160895794162E-4	0.0	0.0	29.338471021837755	0.0
38	2.579160895794162E-4	0.0	0.0	29.55692594971152	0.0
39	2.579160895794162E-4	0.0	0.0	29.778733786749818	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	30	2.161767E-6	45.000004	5
GCGTATG	30	2.161767E-6	45.000004	1
ATTCGGT	20	7.027498E-4	45.0	28
TACGGGT	20	7.027498E-4	45.0	4
TACGGAT	20	7.027498E-4	45.0	29
TCGATCA	20	7.027498E-4	45.0	17
ACGGATC	20	7.027498E-4	45.0	30
GCTTACG	20	7.027498E-4	45.0	1
ATAACGG	20	7.027498E-4	45.0	2
ATACGGA	20	7.027498E-4	45.0	28
CACCCGT	40	6.7957444E-9	45.0	16
CTTACGG	35	1.2091368E-7	45.0	2
ACCGGTC	55	1.8189894E-12	44.999996	17
CGTTTTT	6170	0.0	44.2342	1
TACGGCT	1915	0.0	42.18016	7
CACCGGT	215	0.0	41.860466	16
ACCGGTG	140	0.0	41.785713	17
ACGGCTG	1955	0.0	41.547318	8
ATGACTC	65	0.0	41.538464	3
GACGGGT	60	3.6379788E-12	41.250004	4
>>END_MODULE