FastQCFastQC Report
Sat 18 Jun 2016
SRR3552104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332338
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC158034.75509872479223No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG156164.698830708495567No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155064.665731875379884No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC108673.269863813346653No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT41931.2616673386732784No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC28860.8683930215623853No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCT19410.5840439552503777No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTC16770.5046067557727374No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTA15310.46067557727373937No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCTGTCTC13320.4007967791826394No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTT13030.39207072317941377No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCT11430.34392696592023786No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGT5770.1736184246159031No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTT5530.16639686102702672No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCC4910.14774115508909602No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGACGGT4710.14172318543169907No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTAT4450.133899824877083No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATG4270.12848365218542568No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCTGTCTCT4020.12096119011367945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT253.8848004E-545.0000045
TGGGTGC253.8848004E-545.0000046
TAGCACG302.1608284E-645.0000041
GCGAAGG351.2084456E-745.02
CGATGTG207.026041E-445.010
TGCACGG207.026041E-445.02
CGTTTTT80200.043.961971
TACGGCT13200.043.2954567
GAATCTG20700.042.9347841
AATCTGT20950.042.744632
ACGGCTG13600.042.352948
TGATACC21000.042.3214264
GATACCT21100.042.0142175
ATACCTG21150.041.9148946
CGGCTGT13650.041.8681349
GAATGAT43850.041.6647641
TACCTGT21450.041.3286747
AATGATA44100.041.173472
ATGATAC44300.041.0383763
ATAGCGG556.002665E-1140.9090922
Produced by FastQC (version 0.11.5)