SRR3552103_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467751
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	27620	5.904851085299658	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	24369	5.2098231751508814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24169	5.167065383077748	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	17983	3.844566874255747	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	6932	1.4819850732547872	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	6857	1.4659509012273624	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT	3809	0.8143221500328167	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA	3420	0.7311582444505731	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	3068	0.6559045304018591	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	3024	0.6464978161457698	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	2814	0.6016021344689803	No Hit
GAATGCTGCCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	1656	0.3540345183655406	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1304	0.27878080431682667	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGT	1255	0.26830514525890914	No Hit
GAACTGTCCCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	1182	0.2526985511522156	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT	1063	0.22725766486870153	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATG	908	0.19412037601202348	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	791	0.16910706764924072	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCAGCTCT	753	0.16098308715534546	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	691	0.14772817161267426	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCTCTGT	654	0.13981798007914467	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	647	0.13832145735658502	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	581	0.12421138597245115	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCG	576	0.12314244117062284	No Hit
GACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	558	0.11929423988404086	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	550	0.11758392820111555	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCCTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	522	0.11159783731087694	No Hit
GAATGATCCGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	487	0.10411522369807868	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	25	3.887246E-5	45.000004	2
TAGCCGT	25	3.887246E-5	45.000004	44
GTAGCCG	25	3.887246E-5	45.000004	43
CGGACGG	25	3.887246E-5	45.000004	2
GATACGC	40	6.7993824E-9	45.0	5
CGTTAGG	35	1.2098644E-7	45.0	2
TCTACGG	20	7.028993E-4	45.0	2
ACGTGCG	20	7.028993E-4	45.0	1
TTAACGG	20	7.028993E-4	45.0	2
ATAACGT	20	7.028993E-4	45.0	31
CGCACGG	45	3.8380676E-10	45.0	2
CAGTAGG	35	1.2098644E-7	45.0	2
CGTTTTT	11720	0.0	44.232082	1
TACGGCT	2130	0.0	43.415497	7
CACCGGT	135	0.0	43.333336	16
ACGGCTG	2140	0.0	43.212616	8
CGACCCT	105	0.0	42.857143	12
ACCGGTG	95	0.0	42.63158	17
AACGGGA	90	0.0	42.5	4
CGGCTGT	2175	0.0	42.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content