##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467751
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.56422968630746	28.0	16.0	30.0	16.0	31.0
2	25.117117868267517	28.0	16.0	31.0	16.0	33.0
3	25.875936128410203	30.0	16.0	31.0	16.0	33.0
4	26.736006978071668	28.0	19.0	35.0	19.0	35.0
5	29.925323516144275	32.0	28.0	35.0	19.0	35.0
6	30.0077177814692	33.0	28.0	35.0	17.0	35.0
7	30.80281175240673	32.0	28.0	35.0	25.0	35.0
8	31.51255261880787	35.0	31.0	35.0	18.0	37.0
9	32.942456563427974	34.0	32.0	37.0	25.0	39.0
10	32.59659733490682	35.0	30.0	38.0	23.0	39.0
11	33.38044173074991	35.0	32.0	39.0	26.0	39.0
12	33.29802181074974	35.0	31.0	39.0	25.0	39.0
13	33.59173791183771	35.0	32.0	39.0	27.0	39.0
14	33.75090593071955	36.0	32.0	39.0	25.0	41.0
15	33.83718901723353	36.0	32.0	39.0	25.0	41.0
16	34.10850003527518	36.0	32.0	40.0	25.0	41.0
17	32.339642245553726	34.0	27.0	39.0	19.0	41.0
18	32.71109415052025	35.0	30.0	39.0	21.0	39.0
19	32.42946567725136	35.0	30.0	37.0	19.0	39.0
20	31.408127401117262	34.0	29.0	35.0	21.0	39.0
21	32.84365399539499	35.0	31.0	37.0	25.0	39.0
22	33.289660524509834	35.0	31.0	37.0	26.0	39.0
23	33.96735228786256	35.0	32.0	38.0	27.0	40.0
24	32.68696592845339	35.0	31.0	37.0	23.0	39.0
25	31.960538833695704	35.0	30.0	37.0	21.0	39.0
26	31.280523184343807	33.0	27.0	37.0	21.0	39.0
27	32.79445046616683	35.0	31.0	37.0	24.0	39.0
28	32.96959493405679	35.0	31.0	38.0	23.0	39.0
29	32.60467855760864	35.0	31.0	38.0	22.0	40.0
30	31.851547083811685	35.0	30.0	37.0	20.0	39.0
31	32.176279687269506	35.0	30.0	37.0	21.0	39.0
32	31.99387280839592	35.0	30.0	37.0	19.0	39.0
33	31.652188878270703	35.0	30.0	38.0	15.0	40.0
34	31.054674388723914	35.0	30.0	38.0	12.0	40.0
35	30.849680706187694	35.0	29.0	38.0	10.0	40.0
36	30.10312751870119	35.0	25.0	38.0	8.0	40.0
37	30.476766484732263	35.0	28.0	38.0	8.0	40.0
38	30.18647100701014	35.0	26.0	38.0	8.0	40.0
39	29.678236925201656	34.0	25.0	37.0	8.0	39.0
40	29.25915497775526	33.0	24.0	37.0	8.0	39.0
41	28.69470722670823	33.0	23.0	37.0	8.0	39.0
42	29.85443751055583	35.0	25.0	38.0	8.0	40.0
43	29.85053799991876	35.0	25.0	38.0	8.0	40.0
44	30.263717234169462	35.0	26.0	38.0	8.0	40.0
45	30.503748789420012	35.0	27.0	38.0	8.0	40.0
46	30.346220531864176	35.0	26.0	38.0	8.0	40.0
47	30.182958454391333	35.0	26.0	38.0	8.0	40.0
48	30.252799031963587	35.0	27.0	38.0	8.0	40.0
49	30.410778384225797	35.0	27.0	38.0	8.0	40.0
50	30.341472279054454	35.0	27.0	38.0	8.0	40.0
51	28.975886743160356	33.0	24.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	19.0
10	39.0
11	28.0
12	38.0
13	66.0
14	91.0
15	186.0
16	340.0
17	660.0
18	1340.0
19	2254.0
20	3580.0
21	5239.0
22	7478.0
23	10543.0
24	14796.0
25	19789.0
26	21980.0
27	21955.0
28	22273.0
29	25019.0
30	30634.0
31	38095.0
32	46969.0
33	56676.0
34	62624.0
35	49203.0
36	20662.0
37	4812.0
38	352.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.663502590053255	8.958826384123176	12.081855517144806	32.29581550867876
2	41.615945235819915	34.33343808992391	11.617933473151314	12.432683201104862
3	15.881099131803031	34.06363642194245	39.38270575584018	10.672558690414343
4	15.04582566365438	12.323009464437275	60.60232901693422	12.02883585497412
5	30.246648323573865	13.624342866183076	40.53246278468672	15.596546025556332
6	14.003176903951035	30.648357780100948	43.019469760620495	12.328995555327515
7	42.492479973319135	2.3796849178302133	51.628109827664716	3.4997252811859303
8	38.447165265280034	17.55677700314911	36.57929111856522	7.416766613005637
9	34.64535618309742	2.8660548026621	35.087899331054345	27.40068968318614
10	26.93698142815301	12.901308602226399	42.50680383366364	17.654906135956953
11	22.999630145098568	13.533482558027668	46.928387111946314	16.53850018492745
12	18.15111031296566	10.973787335569565	49.823517213218146	21.05158513824663
13	16.396758104205013	14.350370175584873	58.31542850790271	10.937443212307404
14	14.266351114161166	21.769060889233803	44.221391295796266	19.743196700808763
15	9.80778234573523	15.583504898974027	59.07865509640813	15.53005765888261
16	11.632471122456177	18.92588150533083	41.691840316749726	27.749807055463272
17	12.065607556157016	19.114229579412978	50.21475101068731	18.605411853742694
18	13.139683293034116	17.32353324739017	48.2192448546342	21.317538604941518
19	11.868494134699873	22.286643962279076	42.48820419411182	23.35665770890923
20	14.21140735134719	19.133684374806254	53.39977894221498	13.255129331631574
21	14.774955050871084	26.08930820030315	44.90915038129261	14.226586367533153
22	12.165660789608147	14.248820419411182	48.078357929753224	25.507160861227447
23	13.838559404469471	24.7353827143074	46.38108737341021	15.044970507812918
24	18.739243742931603	15.932622271251157	41.701674608926545	23.626459376890697
25	12.62552084335469	29.659797627370114	39.58302601170281	18.131655517572383
26	14.006597527316886	15.908250329769471	46.68488148608982	23.400270656823825
27	20.332826653497264	17.404773052329123	39.38356091168164	22.878839382491968
28	12.057055997742388	18.55645418181896	47.90946465106435	21.477025169374304
29	20.749073759329214	19.790016483128845	40.39948605133928	19.06142370620266
30	19.287612426269533	21.16403813139897	42.22951955206937	17.318829890262126
31	18.328982727989892	14.300557347819673	40.03005872782741	27.340401196363022
32	21.617056938413814	19.588413493504024	42.19852015281635	16.596009415265815
33	14.284095597871518	14.204779893575855	38.95662435783141	32.55450015072122
34	19.643357256317998	22.806578713888374	41.812417290395956	15.73764673939767
35	25.86996072696798	17.721822080551405	36.101686581108325	20.30653061137229
36	17.62005853541735	21.23266438767635	38.02963542568589	23.117641651220413
37	20.490816695207492	17.42850362692971	45.489801197645754	16.59087848021704
38	18.992583660964915	18.99236987200455	31.442156189938665	30.572890277091876
39	21.416736682551186	17.225404114582332	44.35800244146993	16.999856761396558
40	26.22014704404694	17.51530194483817	36.56218800173597	19.70236300937892
41	17.49306789296014	22.028386898157354	42.67740742403544	17.801137784847064
42	22.65970569811716	18.08847014757852	42.32230396086807	16.92952019343625
43	22.799951256117037	17.455441035935788	36.059783944876656	23.684823763070522
44	24.36787949143882	16.686228356540127	35.79853383530981	23.14735831671124
45	18.629570006264014	15.159347601608546	36.831775880757064	29.379306511370366
46	25.383377053175728	21.56211317559984	35.015852451411114	18.03865731981332
47	15.553574444522834	15.774418440580565	49.248424909834505	19.423582205062097
48	21.0934877744783	19.54800737999491	34.85657967593869	24.501925169588095
49	17.297023416304828	15.185429854773158	44.77467712522261	22.742869603699404
50	19.02294169333684	14.945131063322153	40.645557144720165	25.386370098620848
51	20.19664308574434	15.104190049834207	35.77565841655069	28.923508447870773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	403.0
2	716.0
3	19342.5
4	37969.0
5	25164.5
6	12360.0
7	11398.5
8	10437.0
9	10068.5
10	9700.0
11	9199.0
12	8698.0
13	8276.0
14	7854.0
15	7290.0
16	6726.0
17	6181.5
18	5637.0
19	5135.0
20	4633.0
21	4284.5
22	3936.0
23	3735.5
24	3535.0
25	3452.5
26	3257.5
27	3145.0
28	3215.5
29	3286.0
30	3445.0
31	3604.0
32	3911.0
33	4218.0
34	4582.0
35	4946.0
36	5272.0
37	5598.0
38	5912.5
39	6227.0
40	6764.0
41	7301.0
42	8194.0
43	9087.0
44	12181.0
45	15275.0
46	63055.5
47	110836.0
48	74868.0
49	38900.0
50	35964.0
51	33028.0
52	26912.0
53	20796.0
54	18758.0
55	16720.0
56	15251.5
57	13783.0
58	12593.5
59	11404.0
60	10741.5
61	10079.0
62	9305.5
63	8532.0
64	7861.0
65	7190.0
66	6248.5
67	5307.0
68	4525.0
69	3743.0
70	3314.5
71	2886.0
72	2534.5
73	2183.0
74	1870.0
75	1372.0
76	1187.0
77	905.0
78	623.0
79	485.0
80	347.0
81	260.5
82	174.0
83	126.5
84	79.0
85	54.0
86	29.0
87	19.5
88	10.0
89	8.0
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	467751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.97912075082907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.07939950496952	40.3405566150613
2	9.622128255497227	9.233225069009315
3	3.2599687199548804	4.6923129857592265
4	1.3062738907994125	2.5069549096128125
5	0.5751964092269245	1.3798708986870953
6	0.27747026707487915	0.7987667669250251
7	0.15272925139359364	0.512947063635787
8	0.10727213893981108	0.41174583259143266
9	0.0769405535540139	0.3322386098643297
>10	0.4319188738113479	3.959987617967945
>50	0.06109287935903364	2.01678588722168
>100	0.03720694156451801	3.454608106457848
>500	0.005052794533453063	1.5936377172438085
>1k	0.004593449575866422	4.979704635752158
>5k	9.186899151732843E-4	3.0389531853235896
>10k+	0.0018373798303465685	20.74770409888665
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	27620	5.904851085299658	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	24369	5.2098231751508814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24169	5.167065383077748	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	17983	3.844566874255747	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	6932	1.4819850732547872	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	6857	1.4659509012273624	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT	3809	0.8143221500328167	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA	3420	0.7311582444505731	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	3068	0.6559045304018591	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	3024	0.6464978161457698	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	2814	0.6016021344689803	No Hit
GAATGCTGCCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	1656	0.3540345183655406	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1304	0.27878080431682667	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGT	1255	0.26830514525890914	No Hit
GAACTGTCCCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	1182	0.2526985511522156	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT	1063	0.22725766486870153	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATG	908	0.19412037601202348	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	791	0.16910706764924072	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCAGCTCT	753	0.16098308715534546	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	691	0.14772817161267426	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCTCTGT	654	0.13981798007914467	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	647	0.13832145735658502	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	581	0.12421138597245115	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCG	576	0.12314244117062284	No Hit
GACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	558	0.11929423988404086	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	550	0.11758392820111555	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCCTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	522	0.11159783731087694	No Hit
GAATGATCCGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	487	0.10411522369807868	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12891474310049578	0.0
2	0.0	0.0	0.0	1.869156880477006	0.0
3	0.0	0.0	0.0	2.27086633700409	0.0
4	0.0	0.0	0.0	4.1960359250969	0.0
5	0.0	0.0	0.0	10.398908821146293	0.0
6	0.0	0.0	0.0	11.706655891703065	0.0
7	0.0	0.0	0.0	13.035781858296401	0.0
8	0.0	0.0	0.0	14.500663814721936	0.0
9	0.0	0.0	0.0	15.17067841650793	0.0
10	0.0	0.0	0.0	22.2665478000047	0.0
11	0.0	0.0	0.0	23.09775927790641	0.0
12	0.0	0.0	0.0	27.880218321286325	0.0
13	0.0	0.0	0.0	28.490799592090664	0.0
14	0.0	0.0	0.0	28.9170947790598	0.0
15	0.0	0.0	0.0	29.924682149263177	0.0
16	0.0	0.0	0.0	30.470057787155987	0.0
17	0.0	0.0	0.0	30.918800814963518	0.0
18	0.0	0.0	0.0	31.37673676806677	0.0
19	0.0	0.0	0.0	32.561127608492555	0.0
20	0.0	0.0	0.0	32.95556824036721	0.0
21	0.0	0.0	0.0	33.188384418205416	0.0
22	0.0	0.0	0.0	33.656368452445854	0.0
23	0.0	0.0	0.0	33.87764002642432	0.0
24	0.0	0.0	0.0	34.089932464067424	0.0
25	0.0	0.0	0.0	34.2718668693386	0.0
26	0.0	0.0	0.0	34.52755846593593	0.0
27	0.0	0.0	0.0	34.78816720862168	0.0
28	0.0	0.0	0.0	34.945302094490444	0.0
29	0.0	0.0	0.0	35.11312642837749	0.0
30	0.0	0.0	0.0	35.34401850557241	0.0
31	0.0	0.0	0.0	35.54305602767284	0.0
32	0.0	0.0	0.0	35.73119031279463	0.0
33	0.0	0.0	0.0	35.931938146577984	0.0
34	0.0	0.0	0.0	36.13247219140098	0.0
35	0.0	0.0	0.0	36.33108213558068	0.0
36	0.0	0.0	0.0	36.50083057011102	0.0
37	0.0	0.0	0.0	36.681696030580376	0.0
38	0.0	0.0	0.0	36.90553307208322	0.0
39	0.0	0.0	0.0	37.21894768797929	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	25	3.887246E-5	45.000004	2
TAGCCGT	25	3.887246E-5	45.000004	44
GTAGCCG	25	3.887246E-5	45.000004	43
CGGACGG	25	3.887246E-5	45.000004	2
GATACGC	40	6.7993824E-9	45.0	5
CGTTAGG	35	1.2098644E-7	45.0	2
TCTACGG	20	7.028993E-4	45.0	2
ACGTGCG	20	7.028993E-4	45.0	1
TTAACGG	20	7.028993E-4	45.0	2
ATAACGT	20	7.028993E-4	45.0	31
CGCACGG	45	3.8380676E-10	45.0	2
CAGTAGG	35	1.2098644E-7	45.0	2
CGTTTTT	11720	0.0	44.232082	1
TACGGCT	2130	0.0	43.415497	7
CACCGGT	135	0.0	43.333336	16
ACGGCTG	2140	0.0	43.212616	8
CGACCCT	105	0.0	42.857143	12
ACCGGTG	95	0.0	42.63158	17
AACGGGA	90	0.0	42.5	4
CGGCTGT	2175	0.0	42.0	9
>>END_MODULE