Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552098_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 269297 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC | 10044 | 3.7297110625071945 | Illumina PCR Primer Index 9 (95% over 21bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 7230 | 2.6847681184714274 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG | 7074 | 2.62683951176582 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC | 5617 | 2.085801178624344 | No Hit |
GAACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCT | 2502 | 0.9290857306245521 | Illumina PCR Primer Index 9 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC | 1816 | 0.6743483960088675 | Illumina PCR Primer Index 9 (95% over 24bp) |
GAATGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCT | 1305 | 0.48459507532575563 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTC | 1244 | 0.46194350475497314 | No Hit |
GAATGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTT | 1014 | 0.37653594358644915 | No Hit |
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTA | 774 | 0.28741501019320675 | No Hit |
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGT | 288 | 0.10694512007189089 | No Hit |
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGAACT | 288 | 0.10694512007189089 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCAAGCG | 25 | 3.8828235E-5 | 45.000004 | 1 |
GGTACCT | 55 | 1.8189894E-12 | 45.0 | 8 |
GCGTTTG | 20 | 7.023656E-4 | 45.0 | 1 |
AACAGCG | 20 | 7.023656E-4 | 45.0 | 1 |
GCGTATG | 20 | 7.023656E-4 | 45.0 | 1 |
GCGTAAG | 20 | 7.023656E-4 | 45.0 | 1 |
TGGTCGG | 20 | 7.023656E-4 | 45.0 | 2 |
CGTTTTT | 4650 | 0.0 | 44.322582 | 1 |
GAATCTG | 1355 | 0.0 | 42.509224 | 1 |
AATCTGT | 1340 | 0.0 | 42.31343 | 2 |
TACGGCT | 610 | 0.0 | 42.049183 | 7 |
ATGGGCG | 60 | 3.6379788E-12 | 41.249996 | 5 |
AGTAGCA | 60 | 3.6379788E-12 | 41.249996 | 28 |
TACCGGG | 55 | 6.002665E-11 | 40.909092 | 3 |
TGCCAGT | 50 | 1.0750227E-9 | 40.500004 | 24 |
AGGGTAC | 50 | 1.0750227E-9 | 40.500004 | 6 |
GTTAAGG | 45 | 1.918852E-8 | 40.0 | 2 |
GATACCT | 840 | 0.0 | 39.910713 | 5 |
TGATACC | 860 | 0.0 | 39.76744 | 4 |
ACGGCTG | 655 | 0.0 | 39.160305 | 8 |