FastQCFastQC Report
Sat 18 Jun 2016
SRR3552098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269297
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC100443.7297110625071945Illumina PCR Primer Index 9 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72302.6847681184714274No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG70742.62683951176582No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC56172.085801178624344No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCT25020.9290857306245521Illumina PCR Primer Index 9 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC18160.6743483960088675Illumina PCR Primer Index 9 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCT13050.48459507532575563No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTC12440.46194350475497314No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTT10140.37653594358644915No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTA7740.28741501019320675No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGT2880.10694512007189089No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGAACT2880.10694512007189089No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGCG253.8828235E-545.0000041
GGTACCT551.8189894E-1245.08
GCGTTTG207.023656E-445.01
AACAGCG207.023656E-445.01
GCGTATG207.023656E-445.01
GCGTAAG207.023656E-445.01
TGGTCGG207.023656E-445.02
CGTTTTT46500.044.3225821
GAATCTG13550.042.5092241
AATCTGT13400.042.313432
TACGGCT6100.042.0491837
ATGGGCG603.6379788E-1241.2499965
AGTAGCA603.6379788E-1241.24999628
TACCGGG556.002665E-1140.9090923
TGCCAGT501.0750227E-940.50000424
AGGGTAC501.0750227E-940.5000046
GTTAAGG451.918852E-840.02
GATACCT8400.039.9107135
TGATACC8600.039.767444
ACGGCTG6550.039.1603058