##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269297
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.661414720550173	28.0	25.0	31.0	16.0	33.0
2	27.608135255869914	30.0	25.0	31.0	16.0	33.0
3	27.87267960653108	31.0	26.0	33.0	16.0	33.0
4	30.746781434624225	33.0	28.0	35.0	19.0	37.0
5	31.7566775716031	33.0	30.0	35.0	26.0	35.0
6	31.94778998652046	35.0	32.0	35.0	25.0	37.0
7	32.966319713921806	35.0	32.0	35.0	28.0	37.0
8	33.77223288785245	35.0	33.0	35.0	30.0	37.0
9	34.88537562616739	37.0	34.0	39.0	29.0	39.0
10	35.14967860763395	37.0	34.0	39.0	30.0	39.0
11	35.394152181420516	37.0	34.0	39.0	30.0	39.0
12	34.45152006892019	35.0	32.0	39.0	27.0	39.0
13	35.06020861725158	37.0	34.0	39.0	30.0	39.0
14	36.51746213288674	38.0	35.0	40.0	31.0	41.0
15	36.3930084627753	38.0	35.0	40.0	30.0	41.0
16	36.65621971280779	38.0	35.0	40.0	31.0	41.0
17	35.29459667207581	38.0	33.0	40.0	27.0	41.0
18	35.03074300864844	37.0	33.0	39.0	27.0	40.0
19	34.641314236697774	37.0	33.0	39.0	27.0	40.0
20	34.78271945101505	36.0	34.0	39.0	27.0	40.0
21	35.63064943166838	37.0	34.0	39.0	30.0	40.0
22	36.22224904102162	38.0	35.0	40.0	31.0	40.0
23	36.27051545319851	38.0	35.0	40.0	31.0	41.0
24	36.31633846645154	38.0	35.0	40.0	31.0	41.0
25	35.68281859805345	37.0	35.0	40.0	30.0	41.0
26	35.64392845074397	37.0	35.0	40.0	30.0	41.0
27	36.14717579475449	38.0	35.0	40.0	31.0	41.0
28	36.085429841401876	38.0	35.0	40.0	31.0	41.0
29	36.114576099993684	38.0	35.0	40.0	31.0	41.0
30	35.76177974503986	38.0	35.0	40.0	30.0	41.0
31	35.61685054048133	38.0	35.0	40.0	29.0	41.0
32	35.22891825753722	38.0	35.0	40.0	27.0	41.0
33	34.72922089737353	38.0	34.0	40.0	22.0	41.0
34	33.84585420557971	37.0	33.0	40.0	18.0	41.0
35	33.7827565847373	38.0	33.0	40.0	16.0	41.0
36	33.5282123454773	38.0	33.0	40.0	15.0	41.0
37	33.43086257923408	38.0	33.0	40.0	12.0	41.0
38	33.360018863930904	38.0	33.0	40.0	12.0	41.0
39	32.773328332658735	37.0	32.0	40.0	10.0	40.0
40	32.59821312528547	37.0	32.0	40.0	10.0	41.0
41	32.37983713149423	37.0	31.0	40.0	10.0	41.0
42	32.60038916140915	37.0	32.0	40.0	10.0	41.0
43	32.63154435437454	37.0	32.0	40.0	10.0	41.0
44	32.4919326988418	37.0	32.0	40.0	10.0	40.0
45	32.56747383000925	37.0	32.0	40.0	10.0	40.0
46	32.243593504569304	36.0	31.0	39.0	10.0	40.0
47	32.24531279590935	36.0	31.0	39.0	10.0	40.0
48	32.32066083172111	36.0	31.0	39.0	10.0	40.0
49	32.23895921603285	36.0	31.0	39.0	10.0	40.0
50	32.124279141616874	35.0	31.0	39.0	10.0	40.0
51	29.57507138958102	34.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	5.0
11	10.0
12	9.0
13	16.0
14	33.0
15	54.0
16	146.0
17	265.0
18	431.0
19	666.0
20	990.0
21	1356.0
22	1962.0
23	2830.0
24	4182.0
25	6412.0
26	8629.0
27	9385.0
28	8556.0
29	7834.0
30	8735.0
31	10457.0
32	13840.0
33	18020.0
34	23962.0
35	32777.0
36	40915.0
37	41884.0
38	22779.0
39	2151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.361385384909596	13.545267864105432	17.120131304841866	28.973215446143108
2	36.99150009097762	30.35978863485297	19.823466284436886	12.825244989732527
3	23.683516712031697	28.75524049655213	35.3947500343487	12.166492757067477
4	20.882891380149054	18.47291280630679	46.716450610292725	13.92774520325143
5	25.874777661838046	21.98204955866571	34.97105426350832	17.172118515987925
6	18.92594421772244	33.57222694645689	36.33980326553953	11.16202557028114
7	62.429956516411245	3.7943237392172953	30.369443402637238	3.4062763417342192
8	59.76783996851061	9.872371396636428	25.40280805207633	4.956980582776637
9	55.319220043297925	4.687018422039607	24.54576174261132	15.447999792051156
10	32.760483778133434	17.404204280032825	33.00370965885249	16.831602282981244
11	23.945309453874348	18.526014029120265	37.54107918023595	19.98759733676944
12	20.577652183277202	16.283508542612804	43.64586311767305	19.492976156436946
13	18.359283616230407	17.428341199493495	48.63626405047216	15.576111133803941
14	17.349617708329465	23.296211988993566	39.63022239386254	19.72394790881443
15	15.248591703583775	19.25012161294036	48.98235034181591	16.518936341659952
16	17.382295383906985	21.336665466009645	37.814383375975225	23.46665577410814
17	17.750290571376585	19.5687289498212	40.87754412414546	21.803436354656753
18	17.91442162370914	21.511936635016358	39.55372692603334	21.019914815241165
19	18.463258038522522	22.210421950485895	36.734534733027104	22.591785277964476
20	19.587295810944795	21.779299435196084	41.50176199512063	17.131642758738494
21	20.35782054757387	23.735132585955284	38.53514892479307	17.371897941677776
22	18.583199961380927	17.97272156763722	38.2960077535212	25.148070717460648
23	19.275372544068446	23.37567815460254	39.46534866708504	17.883600634243976
24	22.671622780795925	19.167313412329136	35.630549170618316	22.530514636256623
25	17.966408834855198	27.64457086413885	34.536589713216266	19.85243058778969
26	18.686803046450574	19.810840818872844	37.198334923894436	24.30402121078215
27	22.82387104200938	20.969784290207468	34.561469307121875	21.644875360661278
28	16.851654492994722	20.57431014827495	39.566352391597384	23.007682967132943
29	21.755162515735414	23.595881127528344	33.86706870109953	20.781887655636712
30	23.467398448553084	22.38235108449036	35.72672551123852	18.423524955718037
31	21.562809834495	21.82274589022529	31.88041456087517	24.734029714404542
32	23.117598784984608	28.025191517172487	31.47788501171569	17.379324686127212
33	23.848390438809197	21.74253705017137	30.695106146745044	23.713966364274388
34	21.59920088229724	29.829519081163173	30.27289572479456	18.298384311745025
35	22.64637184966784	27.260979513325434	28.62341578257463	21.469232854432097
36	20.955673475753535	26.366428144390763	27.65422563192312	25.02367274793258
37	22.233073521056674	27.42696725177035	31.28404698158539	19.055912245587585
38	23.209319078935152	24.550589126503453	27.10427520544232	25.135816589119074
39	23.584369673631716	28.308521817918507	28.529467465289255	19.577641043160526
40	23.139879018332916	26.185215579824504	31.75378856801227	18.921116833830308
41	18.983501487205576	29.320787086376754	28.620445084794856	23.075266341622818
42	20.383442815924425	25.81536370624255	33.92536864502761	19.875824832805417
43	24.192620044040595	22.276891313308354	28.2743587934511	25.256129849199954
44	22.324793815007222	22.46478794787911	30.73298254343717	24.4774356936765
45	20.24159199694018	21.84539746079607	31.010371448623637	26.902639093640108
46	25.766347192876264	24.559129882620304	29.84474390728452	19.829779017218907
47	19.313248940760573	21.384567967708517	38.99969179010535	20.30249130142556
48	21.376027211591662	23.382362224607032	30.70995963564392	24.531650928157386
49	18.946739102180864	21.635591930099483	37.400713710141595	22.016955257578065
50	20.76443480618054	20.486303226549126	35.104735663598184	23.64452630367215
51	20.867666554027707	20.664916430558083	31.30669855215617	27.16071846325804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	198.0
2	339.0
3	6355.0
4	12371.0
5	8756.0
6	5141.0
7	4763.5
8	4386.0
9	4286.5
10	4187.0
11	4007.5
12	3828.0
13	3710.5
14	3593.0
15	3219.0
16	2845.0
17	2690.5
18	2536.0
19	2338.5
20	2141.0
21	2024.0
22	1907.0
23	1861.5
24	1816.0
25	1816.5
26	1901.5
27	1986.0
28	2104.0
29	2222.0
30	2531.0
31	2840.0
32	3119.5
33	3399.0
34	3969.0
35	4539.0
36	4958.5
37	5378.0
38	5768.0
39	6158.0
40	6903.5
41	7649.0
42	8139.5
43	8630.0
44	9896.0
45	11162.0
46	27396.0
47	43630.0
48	30808.0
49	17986.0
50	17578.0
51	17170.0
52	15344.0
53	13518.0
54	13001.5
55	12485.0
56	11734.5
57	10984.0
58	10938.5
59	10893.0
60	10397.5
61	9902.0
62	9299.5
63	8697.0
64	7864.5
65	7032.0
66	6207.5
67	5383.0
68	4567.0
69	3751.0
70	3188.0
71	2625.0
72	2160.5
73	1696.0
74	1387.5
75	907.0
76	735.0
77	553.0
78	371.0
79	289.5
80	208.0
81	151.0
82	94.0
83	79.0
84	64.0
85	45.5
86	27.0
87	24.5
88	22.0
89	14.5
90	7.0
91	6.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269297.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.072315582115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.46880953616589	38.032945416554796
2	11.317454177036026	11.560171826314193
3	5.794126459776796	8.877583652292097
4	3.4487722554079996	7.0454713999615945
5	2.0046926894364985	5.119214884002985
6	1.1226084599534778	3.440052812513767
7	0.6434454951224595	2.300357597074915
8	0.3636745819210861	1.4858962413653956
9	0.24635965228564366	1.132394211740926
>10	0.5421148043233043	4.459680294528541
>50	0.027289998109545136	0.9522794227822259
>100	0.01327621529653547	1.046075932383798
>500	7.375675164741929E-4	0.2915602346651277
>1k	0.0036878375823709646	2.9687160328112037
>5k	0.0022127025494225786	7.504097460935412
>10k+	7.375675164741929E-4	3.7835025800730526
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	10044	3.7297110625071945	Illumina PCR Primer Index 9 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7230	2.6847681184714274	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG	7074	2.62683951176582	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	5617	2.085801178624344	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCT	2502	0.9290857306245521	Illumina PCR Primer Index 9 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	1816	0.6743483960088675	Illumina PCR Primer Index 9 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCT	1305	0.48459507532575563	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTC	1244	0.46194350475497314	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTT	1014	0.37653594358644915	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTA	774	0.28741501019320675	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGT	288	0.10694512007189089	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGAACT	288	0.10694512007189089	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1062024456269472	0.0
2	0.0	0.0	0.0	0.9710468367638704	0.0
3	0.0	0.0	0.0	1.1771389952357434	0.0
4	0.0	0.0	0.0	2.396981771055749	0.0
5	0.0	0.0	0.0	7.052436529185249	0.0
6	0.0	0.0	0.0	7.854153592501959	0.0
7	0.0	0.0	0.0	8.734965484205171	0.0
8	0.0	0.0	0.0	9.701927611521851	0.0
9	0.0	0.0	0.0	10.033903088411678	0.0
10	0.0	0.0	0.0	13.260452214469526	0.0
11	0.0	0.0	0.0	13.717939672554838	0.0
12	0.0	0.0	0.0	16.414961919367837	0.0
13	0.0	0.0	0.0	16.713517046235197	0.0
14	0.0	0.0	0.0	16.920351879152015	0.0
15	0.0	0.0	0.0	17.372269278900248	0.0
16	0.0	0.0	0.0	17.688277255223788	0.0
17	0.0	0.0	0.0	17.959353427628233	0.0
18	0.0	0.0	0.0	18.260507915052898	0.0
19	0.0	0.0	0.0	18.582828624158456	0.0
20	0.0	0.0	0.0	18.785950084850555	0.0
21	0.0	0.0	0.0	18.970504684419062	0.0
22	0.0	0.0	0.0	19.20333312290891	0.0
23	0.0	0.0	0.0	19.39122975747966	0.0
24	0.0	0.0	0.0	19.55647482147963	0.0
25	0.0	0.0	0.0	19.693126919349268	0.0
26	0.0	0.0	0.0	19.824951633326773	0.0
27	0.0	0.0	0.0	19.992053383439103	0.0
28	0.0	0.0	0.0	20.153585075214355	0.0
29	0.0	0.0	0.0	20.30806135976264	0.0
30	0.0	0.0	0.0	20.484446540436767	0.0
31	0.0	0.0	0.0	20.646720906656963	0.0
32	3.7133722247184337E-4	0.0	0.0	20.79377044675581	0.0
33	3.7133722247184337E-4	0.0	0.0	20.935621265740057	0.0
34	3.7133722247184337E-4	0.0	0.0	21.102351678629915	0.0
35	3.7133722247184337E-4	0.0	0.0	21.252743253731012	0.0
36	3.7133722247184337E-4	0.0	0.0	21.40796221272424	0.0
37	3.7133722247184337E-4	0.0	0.0	21.567637218387134	0.0
38	3.7133722247184337E-4	0.0	0.0	21.728797572939914	0.0
39	3.7133722247184337E-4	0.0	0.0	21.920036242512914	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGCG	25	3.8828235E-5	45.000004	1
GGTACCT	55	1.8189894E-12	45.0	8
GCGTTTG	20	7.023656E-4	45.0	1
AACAGCG	20	7.023656E-4	45.0	1
GCGTATG	20	7.023656E-4	45.0	1
GCGTAAG	20	7.023656E-4	45.0	1
TGGTCGG	20	7.023656E-4	45.0	2
CGTTTTT	4650	0.0	44.322582	1
GAATCTG	1355	0.0	42.509224	1
AATCTGT	1340	0.0	42.31343	2
TACGGCT	610	0.0	42.049183	7
ATGGGCG	60	3.6379788E-12	41.249996	5
AGTAGCA	60	3.6379788E-12	41.249996	28
TACCGGG	55	6.002665E-11	40.909092	3
TGCCAGT	50	1.0750227E-9	40.500004	24
AGGGTAC	50	1.0750227E-9	40.500004	6
GTTAAGG	45	1.918852E-8	40.0	2
GATACCT	840	0.0	39.910713	5
TGATACC	860	0.0	39.76744	4
ACGGCTG	655	0.0	39.160305	8
>>END_MODULE