##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495035
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.468708273152405	28.0	25.0	31.0	16.0	33.0
2	27.448790489561343	30.0	25.0	31.0	16.0	33.0
3	27.626060783580957	30.0	25.0	33.0	16.0	33.0
4	30.67325744644318	33.0	28.0	35.0	19.0	37.0
5	31.695809387215046	33.0	30.0	35.0	26.0	35.0
6	31.99463270273819	35.0	32.0	35.0	25.0	36.0
7	33.05183875887563	35.0	32.0	35.0	28.0	37.0
8	33.687153433595604	35.0	33.0	35.0	30.0	37.0
9	34.854511297181006	37.0	34.0	39.0	29.0	39.0
10	35.24348581413435	37.0	34.0	39.0	30.0	39.0
11	35.587221105578394	37.0	34.0	39.0	31.0	39.0
12	34.61819669316311	37.0	33.0	39.0	28.0	39.0
13	35.166636702455385	37.0	34.0	39.0	30.0	39.0
14	36.54409890209783	38.0	35.0	40.0	32.0	41.0
15	36.4015150443908	38.0	35.0	40.0	30.0	41.0
16	36.68673124122537	38.0	35.0	40.0	31.0	41.0
17	35.311268900178774	37.0	33.0	40.0	27.0	41.0
18	34.9943175735049	37.0	33.0	39.0	27.0	40.0
19	34.67772379730726	37.0	33.0	39.0	27.0	40.0
20	34.82874342218227	36.0	34.0	39.0	27.0	40.0
21	35.66107244942277	37.0	34.0	39.0	30.0	40.0
22	36.256054622400434	38.0	35.0	39.0	31.0	40.0
23	36.31021846940116	38.0	35.0	40.0	31.0	41.0
24	36.35593846899714	38.0	35.0	40.0	31.0	41.0
25	35.70360277556132	37.0	35.0	40.0	30.0	41.0
26	35.7070489965356	37.0	35.0	40.0	30.0	41.0
27	36.25338612421344	38.0	35.0	40.0	31.0	41.0
28	36.160998717262416	38.0	35.0	40.0	31.0	41.0
29	36.23771248497581	38.0	35.0	40.0	31.0	41.0
30	35.92878079327724	38.0	35.0	40.0	30.0	41.0
31	35.76019271364651	38.0	35.0	40.0	30.0	41.0
32	35.402716979607504	38.0	35.0	40.0	27.0	41.0
33	34.86421162140051	38.0	34.0	40.0	23.0	41.0
34	33.98011049723757	38.0	33.0	40.0	18.0	41.0
35	33.827153635601526	38.0	33.0	40.0	16.0	41.0
36	33.74218186592867	38.0	34.0	40.0	15.0	41.0
37	33.56911935519711	38.0	33.0	40.0	15.0	41.0
38	33.565901400911045	38.0	33.0	40.0	15.0	41.0
39	32.90871352530629	37.0	33.0	40.0	12.0	40.0
40	32.71121839869908	37.0	32.0	40.0	10.0	40.0
41	32.474877533911744	37.0	31.0	40.0	10.0	41.0
42	32.724730574605836	37.0	32.0	40.0	10.0	41.0
43	32.845017018998654	37.0	33.0	40.0	10.0	41.0
44	32.660040199177836	37.0	32.0	40.0	10.0	40.0
45	32.70253416425101	37.0	32.0	40.0	10.0	40.0
46	32.29662145100851	36.0	31.0	39.0	10.0	40.0
47	32.26862747078489	36.0	31.0	39.0	10.0	40.0
48	32.37737735715656	36.0	31.0	39.0	10.0	40.0
49	32.29702344278687	36.0	31.0	39.0	10.0	40.0
50	32.22989485591928	36.0	31.0	39.0	10.0	40.0
51	29.588182653751755	34.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	9.0
10	14.0
11	13.0
12	15.0
13	27.0
14	41.0
15	78.0
16	175.0
17	350.0
18	626.0
19	1135.0
20	1659.0
21	2301.0
22	3508.0
23	5007.0
24	7499.0
25	11778.0
26	15430.0
27	16261.0
28	15122.0
29	14361.0
30	15672.0
31	18909.0
32	25030.0
33	33601.0
34	44437.0
35	64252.0
36	79426.0
37	75931.0
38	38711.0
39	3649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52338723524599	12.622339834557152	15.991798559697799	27.862474370499058
2	36.91880372094902	32.590018887553406	18.078923712464775	12.412253679032796
3	22.545880594301412	31.772702940196147	34.10809336713566	11.573323098366782
4	19.88768470916198	17.63612673851344	48.44223135737877	14.033957194945812
5	27.14818144171624	20.22806468229519	34.667447756219254	17.95630611976931
6	18.49263183411274	33.31037199389942	36.948498591008715	11.248497580979123
7	60.43754482006322	3.5785348510711366	32.37165048935934	3.6122698395062978
8	56.43802963426828	11.089720928823214	26.886583776904665	5.585665660003838
9	52.35286393891341	4.386356520246043	25.198218307796417	18.06256123304413
10	29.313078873210983	19.631541204157283	33.84003151292333	17.215348409708405
11	21.86168654741584	18.221135879281263	39.572959487712986	20.344218085589908
12	19.464280303412888	16.181280111507267	44.67987112022382	19.67456846485602
13	17.225650711565848	17.942569717292717	50.504307776217836	14.327471794923591
14	16.54165867059905	24.436655994020626	39.61942084903088	19.40226448634945
15	14.073954366862948	19.403072510024543	50.6186431262436	15.904329996868908
16	16.048966234710676	23.11573929116123	37.408466068055795	23.426828406072296
17	16.465906451059016	20.21917642186916	41.791186481763916	21.523730645307907
18	17.342814144454433	21.94774106881332	40.22463058167604	20.48481420505621
19	16.65700405021867	23.608431727049602	36.43802963426828	23.29653458846344
20	17.953882048744028	23.61125980991243	42.386901936226735	16.047956205116808
21	18.654236569131474	25.38325572939287	39.49215712020362	16.47035058127203
22	17.211308291332937	18.03428040441585	38.75140141606149	26.003009888189727
23	17.660165442847475	25.28265678184371	40.09979092387407	16.957386851434748
24	22.11682002282667	19.256820224832587	35.6566707404527	22.969689011888047
25	16.160675507792376	30.095851808457986	34.47493611562818	19.268536568121448
26	17.70400072722131	19.30065550920642	37.73692769198138	25.2584160715909
27	21.988950276243095	21.80209480137768	33.51096387124143	22.697991051137798
28	15.14014160614906	19.599220257153537	40.13736402476592	25.123274111931483
29	20.329269647600675	18.995020554102236	34.214752492248024	26.460957306049064
30	17.742785863625805	27.333420869231468	35.95220539961821	18.97158786752452
31	19.159857383821347	20.40178977244033	32.950599452563964	27.487753391174362
32	21.11446665387296	23.530457442403062	37.101417071520196	18.253658832203783
33	22.20428858565556	21.14577757128284	31.217388669488017	25.432545173573583
34	20.315533245124083	27.097073944266565	30.90387548355167	21.683517327057682
35	17.585221246982535	31.778359105921805	29.332067429575687	21.304352217519973
36	18.994414536345914	24.033856191986423	27.46129061581504	29.510438655852617
37	17.75510822467098	26.260971446463383	36.29278737867019	19.691132950195442
38	22.224085165695353	23.87043340369873	27.550173220075347	26.35530821053057
39	23.525407294433727	24.54270910137667	31.794519579423675	20.137364024765926
40	22.648701606957083	26.512064803498742	32.593048976335005	18.246184613209167
41	18.11306271273748	30.021109618511822	27.813386932237115	24.05244073651358
42	19.908087306958095	24.90005757168685	35.821709576090576	19.370145545264474
43	24.31949256113204	21.15405981395255	28.2755764743907	26.250871150524713
44	22.509519528922198	21.64695425575969	30.678436878200532	25.16508933711758
45	19.93333804680477	20.237558960477543	31.64867130606927	28.180431686648415
46	26.250871150524713	24.03486622158029	29.363782358823116	20.350480269071884
47	17.89550233821851	21.08961992586383	40.61126991020837	20.403607825709294
48	21.52029654468876	23.02928075792621	30.27684911167897	25.17357358570606
49	18.51687254436555	20.160998717262416	38.82412354681992	22.49800519155211
50	19.924651792297514	19.72830203924975	35.63121799468724	24.71582817376549
51	20.50440877917723	19.37337763996485	30.596220469259748	29.52599311159817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	299.0
2	518.0
3	10848.5
4	21179.0
5	14828.0
6	8477.0
7	7934.0
8	7391.0
9	7246.5
10	7102.0
11	6982.0
12	6862.0
13	6570.5
14	6279.0
15	5861.0
16	5443.0
17	5157.0
18	4871.0
19	4430.0
20	3989.0
21	3865.5
22	3742.0
23	3562.5
24	3383.0
25	3440.0
26	3659.0
27	3821.0
28	4182.0
29	4543.0
30	5124.0
31	5705.0
32	6321.5
33	6938.0
34	7728.0
35	8518.0
36	9335.0
37	10152.0
38	11261.5
39	12371.0
40	13375.0
41	14379.0
42	15569.5
43	16760.0
44	19447.5
45	22135.0
46	59139.0
47	96143.0
48	66700.5
49	37258.0
50	35890.5
51	34523.0
52	30381.0
53	26239.0
54	24125.0
55	22011.0
56	20212.0
57	18413.0
58	16986.5
59	15560.0
60	14734.0
61	13908.0
62	12920.0
63	11932.0
64	10639.0
65	9346.0
66	8178.0
67	7010.0
68	6074.5
69	5139.0
70	4314.5
71	3490.0
72	2875.5
73	2261.0
74	1937.5
75	1296.5
76	979.0
77	757.5
78	536.0
79	397.5
80	259.0
81	194.5
82	130.0
83	99.5
84	69.0
85	54.5
86	40.0
87	26.5
88	13.0
89	12.0
90	11.0
91	10.0
92	9.0
93	4.5
94	0.0
95	1.0
96	2.0
97	2.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.24392845838004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63955000981105	34.222303892679506
2	9.679621591212015	8.758882135539743
3	4.852405422398555	6.586256513461669
4	3.0129656321782905	5.452736060393295
5	1.9835460380188157	4.487170751901323
6	1.4298469820673165	3.8815136737850566
7	0.9662485363418016	3.060181575588403
8	0.6577920112523993	2.380887575807798
9	0.46675178989663507	1.9005916130913767
>10	1.2398558429474338	8.139225327428582
>50	0.04303280464319967	1.3311910996074927
>100	0.02197419811549401	1.9606433715268883
>500	0.0018311831762911678	0.4896430308778327
>1k	0.0022889789703639594	2.9040968426134057
>5k	4.5779579407279194E-4	1.129658667685152
>10k+	0.0018311831762911678	13.315017868012466
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	23180	4.682497197167877	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	17063	3.4468269920308665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12365	2.497803185633339	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	11677	2.358823113517226	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	5454	1.101740280990233	Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	3835	0.7746926984960659	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	3143	0.6349046027048593	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	2908	0.5874332117931055	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	2370	0.4787540274930055	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTA	1765	0.3565404466350864	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCC	818	0.1652408415566576	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTGAACTCGT	530	0.10706313694991262	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATG	508	0.10261900673689739	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTGAACT	508	0.10261900673689739	No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	496	0.10019493571161635	Illumina PCR Primer Index 5 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0200591877342006E-4	0.0	0.0	0.12120355126405204	0.0
2	2.0200591877342006E-4	0.0	0.0	1.1227488965426686	0.0
3	2.0200591877342006E-4	0.0	0.0	1.3732362358217096	0.0
4	2.0200591877342006E-4	0.0	0.0	2.8620198571818154	0.0
5	2.0200591877342006E-4	0.0	0.0	8.843415112062784	0.0
6	2.0200591877342006E-4	0.0	0.0	10.004141121334856	0.0
7	2.0200591877342006E-4	0.0	0.0	11.231529083802155	0.0
8	2.0200591877342006E-4	0.0	0.0	12.55608189319947	0.0
9	2.0200591877342006E-4	0.0	0.0	12.963123819527912	0.0
10	2.0200591877342006E-4	0.0	0.0	17.362206712656683	0.0
11	2.0200591877342006E-4	0.0	0.0	18.16316018059329	0.0
12	2.0200591877342006E-4	0.0	0.0	21.41000131303847	0.0
13	2.0200591877342006E-4	0.0	0.0	21.848556162695566	0.0
14	2.0200591877342006E-4	0.0	0.0	22.099447513812155	0.0
15	2.0200591877342006E-4	0.0	0.0	22.676982435585362	0.0
16	2.0200591877342006E-4	0.0	0.0	23.088064480289272	0.0
17	2.0200591877342006E-4	0.0	0.0	23.510054844606948	0.0
18	2.0200591877342006E-4	0.0	0.0	23.94214550486329	0.0
19	2.0200591877342006E-4	0.0	0.0	24.32292666175119	0.0
20	2.0200591877342006E-4	0.0	0.0	24.605734948033977	0.0
21	2.0200591877342006E-4	0.0	0.0	24.882685062672337	0.0
22	2.0200591877342006E-4	0.0	0.0	25.239225509307424	0.0
23	2.0200591877342006E-4	0.0	0.0	25.51294352924541	0.0
24	2.0200591877342006E-4	0.0	0.0	25.74646237134748	0.0
25	2.0200591877342006E-4	0.0	0.0	25.95432646176533	0.0
26	2.0200591877342006E-4	0.0	0.0	26.165422646883552	0.0
27	2.0200591877342006E-4	0.0	0.0	26.390659246315916	0.0
28	2.0200591877342006E-4	0.0	0.0	26.605997555728383	0.0
29	2.0200591877342006E-4	0.0	0.0	26.825779995353862	0.0
30	2.0200591877342006E-4	0.0	0.0	27.08434757138384	0.0
31	2.0200591877342006E-4	0.0	0.0	27.309786176734978	0.0
32	4.040118375468401E-4	0.0	0.0	27.550577231912897	0.0
33	4.040118375468401E-4	0.0	0.0	27.777429878695447	0.0
34	4.040118375468401E-4	0.0	0.0	28.001858454452716	0.0
35	4.040118375468401E-4	0.0	0.0	28.226287030209985	0.0
36	4.040118375468401E-4	0.0	0.0	28.43697920349066	0.0
37	4.040118375468401E-4	0.0	0.0	28.657367660872463	0.0
38	4.040118375468401E-4	0.0	0.0	28.88523033724888	0.0
39	4.040118375468401E-4	0.0	0.0	29.123597321401515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8100	0.0	43.75	1
TACGGCT	1545	0.0	42.37864	7
GATACCT	1955	0.0	42.007675	5
ACGGCTG	1565	0.0	41.98083	8
GAATCTG	2860	0.0	41.774475	1
TGATACC	2005	0.0	41.29676	4
CGGCTGT	1615	0.0	41.09907	9
AATCTGT	2925	0.0	40.692307	2
CGCACGA	50	1.0786607E-9	40.5	39
GAATGAT	4730	0.0	40.433407	1
ATACCTG	2055	0.0	40.182484	6
ATGATAC	4590	0.0	40.049015	3
CGGGCTA	45	1.9244908E-8	40.0	6
AATGATA	4615	0.0	39.73456	2
ATACGGC	2625	0.0	38.82857	6
CTACGGG	70	0.0	38.57143	3
TAGGGCG	70	0.0	38.57143	5
TACCTGT	2160	0.0	38.229164	7
TGATACG	2680	0.0	37.947758	4
GTTTTTT	9490	0.0	37.83983	2
>>END_MODULE