FastQCFastQC Report
Sat 18 Jun 2016
SRR3552094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478464
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC298566.239967897271268No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG233264.875183921883361No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155563.251237292669877No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC148223.097829721776351No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT61201.2790930979133226No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC48721.0182584269662922No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT37560.7850120385232745No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC31560.6596107544141252No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT31340.6550127073301231No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTA16890.35300461476725525No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCC5820.1216392455858748No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT5790.12101223916532906No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATG5600.11704119850187267No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGATTACGTCGT5410.11307015783841626No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTAT5320.11118913857677902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGC453.8380676E-1045.00000415
TACGGGT253.8873804E-545.04
ACGGGAC406.8012014E-945.05
TTAACGG207.029155E-445.02
CGTTTTT85950.044.1099471
GAATCTG37200.042.3387071
TACGGCT17200.042.2529077
TGATACC27750.042.2432444
AATCTGT37450.042.2363172
GATACCT27700.041.9133575
CACCCGT650.041.5384616
ACGGCTG17500.041.5285728
ATACCTG28050.041.4705856
GAATGAC4700.040.691491
TACCTGT28800.040.546887
ACAACGA501.0786607E-940.513
AGCGACT501.0786607E-940.519
CGGCTGT18200.040.0549439
ATGATAC57000.039.9868433
GAATGAT58600.039.8933451