##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478464
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.796068251738898	28.0	25.0	31.0	16.0	33.0
2	27.653645833333332	30.0	25.0	31.0	16.0	33.0
3	27.810516569689675	30.0	26.0	31.0	16.0	33.0
4	30.67169107811664	33.0	28.0	35.0	19.0	37.0
5	31.731212797619047	33.0	30.0	35.0	26.0	35.0
6	32.17269219836811	35.0	32.0	35.0	26.0	36.0
7	33.05000376203852	35.0	32.0	35.0	28.0	37.0
8	33.501991790395934	35.0	33.0	35.0	28.0	37.0
9	34.682396167736755	37.0	33.0	39.0	28.0	39.0
10	35.05900966425896	37.0	34.0	39.0	30.0	39.0
11	35.4994273341359	37.0	34.0	39.0	30.0	39.0
12	34.59417427434457	37.0	33.0	39.0	28.0	39.0
13	34.967343415596574	37.0	33.0	39.0	30.0	39.0
14	36.50089661918138	38.0	35.0	40.0	31.0	41.0
15	36.057626070090954	38.0	34.0	40.0	29.0	41.0
16	36.5045917770198	38.0	34.0	40.0	31.0	41.0
17	35.142430778491175	37.0	32.0	40.0	27.0	41.0
18	34.77059925093633	37.0	32.0	39.0	27.0	40.0
19	34.37517765181916	37.0	32.0	38.0	27.0	40.0
20	34.37778599852862	35.0	33.0	39.0	27.0	40.0
21	35.40342429106474	36.0	34.0	39.0	30.0	40.0
22	36.01399687332798	37.0	35.0	39.0	31.0	40.0
23	36.07956293472445	37.0	35.0	40.0	31.0	41.0
24	36.147766603130016	38.0	35.0	40.0	31.0	41.0
25	35.57210573836276	37.0	35.0	40.0	30.0	40.0
26	35.428646669341894	37.0	34.0	40.0	29.0	41.0
27	36.02714310794543	37.0	35.0	40.0	31.0	41.0
28	35.868215790529696	38.0	35.0	40.0	30.0	41.0
29	35.95367676565008	38.0	35.0	40.0	31.0	41.0
30	35.682665780497594	38.0	35.0	40.0	30.0	41.0
31	35.26316922485286	37.0	35.0	40.0	27.0	41.0
32	35.00786893057785	37.0	34.0	40.0	25.0	41.0
33	34.38018743311932	37.0	34.0	40.0	21.0	41.0
34	33.890909242910645	37.0	33.0	40.0	18.0	41.0
35	33.62134455256822	38.0	33.0	40.0	15.0	41.0
36	33.13493387172284	37.0	33.0	40.0	12.0	41.0
37	33.09238312600321	37.0	33.0	40.0	10.0	41.0
38	32.90746639245586	37.0	33.0	40.0	10.0	41.0
39	32.65051498127341	37.0	32.0	40.0	10.0	40.0
40	32.387308136035315	37.0	31.0	40.0	10.0	40.0
41	31.943678103263778	36.0	31.0	39.0	10.0	40.0
42	32.193385500267524	37.0	31.0	40.0	10.0	40.0
43	32.31431622859818	37.0	31.0	40.0	10.0	40.0
44	32.213894880283576	37.0	31.0	40.0	10.0	40.0
45	32.22383293204923	37.0	31.0	40.0	10.0	40.0
46	31.85272455189941	36.0	31.0	39.0	10.0	40.0
47	31.75219243245051	36.0	31.0	39.0	10.0	40.0
48	31.87767731741573	36.0	31.0	39.0	10.0	40.0
49	31.816905765115035	36.0	31.0	39.0	10.0	40.0
50	31.773132356875333	35.0	31.0	39.0	10.0	40.0
51	28.849614600053506	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	14.0
11	8.0
12	9.0
13	16.0
14	31.0
15	75.0
16	196.0
17	412.0
18	772.0
19	1128.0
20	1745.0
21	2476.0
22	3591.0
23	5022.0
24	7639.0
25	12294.0
26	16369.0
27	17396.0
28	16337.0
29	15502.0
30	16460.0
31	20073.0
32	26247.0
33	33695.0
34	42946.0
35	60674.0
36	73237.0
37	68122.0
38	33054.0
39	2914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.66396635901552	11.368044408774745	15.122140850722312	28.84584838148743
2	35.647614031567684	36.34254614767255	16.631763309256286	11.378076511503478
3	19.320993846976993	35.06073602193687	35.00890349117175	10.609366639914393
4	18.322590623327983	15.207622726056716	53.93007624398074	12.539710406634564
5	28.451879347244514	18.090807249866238	35.17338817549492	18.28392522739433
6	16.592052902621724	33.69992308721241	39.09217830390583	10.615845706260032
7	55.464151952916	3.3016068084537187	37.53490335741038	3.6993378812199036
8	50.208584135901546	13.58367609684323	30.206452314071697	6.0012874531835205
9	46.54728464419476	3.7540964419475658	27.279795345104336	22.418823568753343
10	29.91844736490102	14.358238028357409	37.39152788924559	18.331786717495987
11	22.273567081326913	16.269980604601393	40.545579186730876	20.910873127340825
12	20.336953250401287	13.155221709470306	46.952957798287855	19.554867241840558
13	16.228807182985555	15.431464018191546	54.72428437667202	13.615444422150883
14	16.19139579989299	23.4964386035313	40.480788523274484	19.831377073301233
15	12.69332697966827	17.36222578919208	55.24177367576244	14.702673555377208
16	14.766001203852328	21.983472110754416	37.3948719234885	25.855654761904763
17	15.575884497057249	18.167720037453183	44.03507891920813	22.221316546281432
18	15.805995853397539	21.621271401819158	41.92562031835206	20.647112426431246
19	15.38506554307116	22.953451043338685	37.42287821027288	24.23860520331728
20	16.169032570893528	22.698259430176567	45.305393927233816	15.827314071696094
21	16.893015984483682	25.624916399143927	41.7621806447298	15.719886971642591
22	15.446930176565008	16.064113496522204	40.60744382022472	27.88151250668807
23	16.60898207597646	24.386160714285715	42.40005517656501	16.60480203317282
24	22.72898274478331	17.153223649010165	35.701745585874804	24.41604802033173
25	15.26405330390583	31.245401952915998	34.46696094168004	19.023583801498127
26	16.317633092562865	16.607519060995184	39.55532704654895	27.519520799892994
27	23.284092429106472	19.317022806313535	34.064422819689675	23.334461944890318
28	14.718975722311397	17.919425494917068	42.35825474852862	25.003344034242907
29	22.17951611824505	15.405965757089351	34.76353497859818	27.650983146067414
30	23.39277354200107	22.33229668271803	37.77650983146067	16.498419943820224
31	19.93838616907437	24.046741238630283	33.76847578919208	22.246396803103263
32	22.06707296682718	20.84817248528625	40.22685092295345	16.85790362493312
33	18.04064673622258	18.73536985018727	39.204621455323704	24.01936195826645
34	19.71454487693954	25.5304474317817	32.402646803103266	22.352360888175497
35	23.26193820224719	19.404176698769398	30.073526952915998	27.260358146067414
36	25.59126705457464	25.130208333333332	28.768726591760302	20.50979802033173
37	21.760257825040128	20.23287018459069	40.01868479133226	17.98818719903692
38	19.324546883360085	24.000133761369717	32.90864098448368	23.76667837078652
39	24.996446963616908	24.26138643659711	32.87520064205457	17.866965957731406
40	19.370527354200107	19.88801665329053	37.864499732477256	22.876956260032102
41	23.09348247726057	29.63922050561798	28.163038389513105	19.10425862760835
42	19.285045478865705	19.62613697164259	39.298463416265385	21.790354133226327
43	28.48511068753344	18.673087212413055	29.155380551096844	23.686421548956663
44	23.32610185928304	19.235302969502406	32.17734249598716	25.261252675227396
45	18.827121789727126	17.97063101926164	33.31159711075441	29.89065008025682
46	27.895515650080256	22.878001270733012	30.339586677367574	18.886896401819158
47	17.171406835205993	17.98421615837346	45.467370585874804	19.377006420545747
48	21.986816144997327	21.185083935259495	30.85101491439272	25.977085005350453
49	17.286358012306046	17.25082764847512	42.080908908507226	23.381905430711612
50	20.116873996789728	16.91140817281969	37.860528691813805	25.11118913857678
51	20.77167770197967	17.167435794542534	31.745962078651687	30.314924424826113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	98.0
1	329.5
2	561.0
3	12932.5
4	25304.0
5	17261.5
6	9219.0
7	8648.5
8	8078.0
9	7919.0
10	7760.0
11	7476.0
12	7192.0
13	6829.5
14	6467.0
15	5938.0
16	5409.0
17	5136.0
18	4863.0
19	4417.0
20	3971.0
21	3780.0
22	3589.0
23	3393.5
24	3198.0
25	3207.0
26	3263.5
27	3311.0
28	3473.0
29	3635.0
30	4040.5
31	4446.0
32	4814.5
33	5183.0
34	5698.5
35	6214.0
36	6873.5
37	7533.0
38	8047.5
39	8562.0
40	9505.5
41	10449.0
42	11789.5
43	13130.0
44	16108.5
45	19087.0
46	65355.0
47	111623.0
48	72024.5
49	32426.0
50	31463.0
51	30500.0
52	26107.0
53	21714.0
54	20239.5
55	18765.0
56	17963.0
57	17161.0
58	16621.0
59	16081.0
60	14994.5
61	13908.0
62	12982.0
63	12056.0
64	10927.0
65	9798.0
66	8771.0
67	7744.0
68	6934.0
69	6124.0
70	5050.5
71	3977.0
72	3204.0
73	2431.0
74	2029.0
75	1302.5
76	978.0
77	739.5
78	501.0
79	399.0
80	297.0
81	210.0
82	123.0
83	100.0
84	77.0
85	55.5
86	34.0
87	27.0
88	20.0
89	16.5
90	13.0
91	9.5
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	478464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.006964242244415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06614798867089	34.23506402571383
2	10.241975040046109	9.2192040879463
3	5.369288133790384	7.249660771314331
4	3.197410614038127	5.7562298069514695
5	1.9311338388540205	4.345723581614554
6	1.158719417009698	3.1290266020889863
7	0.6872342011081719	2.1651227580726027
8	0.3908959571499143	1.407443229270728
9	0.24860385200610174	1.0070014209950562
>10	0.6375047513759461	4.788451270730515
>50	0.03980827533233819	1.2748440244289103
>100	0.024169310023178375	2.1561650063478695
>500	0.002369540198350821	0.5959367917435895
>1k	0.002369540198350821	3.5421339658145277
>5k	4.7390803967016417E-4	1.3053447263674902
>10k+	0.0018956321586806567	17.82264793059926
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	29856	6.239967897271268	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	23326	4.875183921883361	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15556	3.251237292669877	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	14822	3.097829721776351	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	6120	1.2790930979133226	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	4872	1.0182584269662922	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT	3756	0.7850120385232745	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC	3156	0.6596107544141252	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT	3134	0.6550127073301231	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTA	1689	0.35300461476725525	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCC	582	0.1216392455858748	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT	579	0.12101223916532906	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATG	560	0.11704119850187267	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGATTACGTCGT	541	0.11307015783841626	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTAT	532	0.11118913857677902	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0900214018191546E-4	0.0	0.0	0.11181614499732477	0.0
2	2.0900214018191546E-4	0.0	0.0	1.3662469903691814	0.0
3	2.0900214018191546E-4	0.0	0.0	1.6580139780631353	0.0
4	2.0900214018191546E-4	0.0	0.0	3.2915747057249867	0.0
5	2.0900214018191546E-4	0.0	0.0	10.90489566613162	0.0
6	2.0900214018191546E-4	0.0	0.0	12.208442014446227	0.0
7	2.0900214018191546E-4	0.0	0.0	13.52118445692884	0.0
8	2.0900214018191546E-4	0.0	0.0	14.796097512038523	0.0
9	2.0900214018191546E-4	0.0	0.0	15.209085741037988	0.0
10	2.0900214018191546E-4	0.0	0.0	21.04923254414125	0.0
11	2.0900214018191546E-4	0.0	0.0	21.733923555377206	0.0
12	2.0900214018191546E-4	0.0	0.0	25.674867910647404	0.0
13	2.0900214018191546E-4	0.0	0.0	26.092872191011235	0.0
14	2.0900214018191546E-4	0.0	0.0	26.351616840556446	0.0
15	2.0900214018191546E-4	0.0	0.0	26.910070559122527	0.0
16	2.0900214018191546E-4	0.0	0.0	27.29797853130016	0.0
17	2.0900214018191546E-4	0.0	0.0	27.67292837078652	0.0
18	2.0900214018191546E-4	0.0	0.0	28.10514479668272	0.0
19	2.0900214018191546E-4	0.0	0.0	28.486364700374533	0.0
20	2.0900214018191546E-4	0.0	0.0	28.742810326377743	0.0
21	2.0900214018191546E-4	0.0	0.0	28.980863764044944	0.0
22	2.0900214018191546E-4	0.0	0.0	29.292067950775817	0.0
23	2.0900214018191546E-4	0.0	0.0	29.521761302835742	0.0
24	2.0900214018191546E-4	0.0	0.0	29.707773207597647	0.0
25	2.0900214018191546E-4	0.0	0.0	29.882498996789728	0.0
26	2.0900214018191546E-4	0.0	0.0	30.066420880149813	0.0
27	4.180042803638309E-4	0.0	0.0	30.294651217228463	0.0
28	4.180042803638309E-4	0.0	0.0	30.47000401284109	0.0
29	4.180042803638309E-4	0.0	0.0	30.656015917602996	0.0
30	4.180042803638309E-4	0.0	0.0	30.874423154093098	0.0
31	4.180042803638309E-4	0.0	0.0	31.072557182985555	0.0
32	4.180042803638309E-4	0.0	0.0	31.25647906634564	0.0
33	4.180042803638309E-4	0.0	0.0	31.44980604601391	0.0
34	4.180042803638309E-4	0.0	0.0	31.651493111289458	0.0
35	4.180042803638309E-4	0.0	0.0	31.871154360620654	0.0
36	4.180042803638309E-4	0.0	0.0	32.067825374531836	0.0
37	4.180042803638309E-4	0.0	0.0	32.27431948903157	0.0
38	4.180042803638309E-4	0.0	0.0	32.49042770197967	0.0
39	4.180042803638309E-4	0.0	0.0	32.78804674959871	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGC	45	3.8380676E-10	45.000004	15
TACGGGT	25	3.8873804E-5	45.0	4
ACGGGAC	40	6.8012014E-9	45.0	5
TTAACGG	20	7.029155E-4	45.0	2
CGTTTTT	8595	0.0	44.109947	1
GAATCTG	3720	0.0	42.338707	1
TACGGCT	1720	0.0	42.252907	7
TGATACC	2775	0.0	42.243244	4
AATCTGT	3745	0.0	42.236317	2
GATACCT	2770	0.0	41.913357	5
CACCCGT	65	0.0	41.53846	16
ACGGCTG	1750	0.0	41.528572	8
ATACCTG	2805	0.0	41.470585	6
GAATGAC	470	0.0	40.69149	1
TACCTGT	2880	0.0	40.54688	7
ACAACGA	50	1.0786607E-9	40.5	13
AGCGACT	50	1.0786607E-9	40.5	19
CGGCTGT	1820	0.0	40.054943	9
ATGATAC	5700	0.0	39.986843	3
GAATGAT	5860	0.0	39.893345	1
>>END_MODULE