FastQCFastQC Report
Sat 18 Jun 2016
SRR3552093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572245
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC275784.819264475880086No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG260984.560633994180814No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC214553.749268233012084No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122922.148031000707739No Hit
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT73581.2858128948265166No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC64401.1253920960427788No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACATCCGTT45160.789172469833725No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTA40750.7121075763003608No Hit
GAATGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT36710.6415084448094786No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC33400.5836660870780872No Hit
GAATGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT24180.4225462869924595No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATG12580.21983590944438133No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACATCCGTTCGT11050.1930991096470917No Hit
GAATCTTTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC10980.19187585736878435No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTAT9360.1635663046422424No Hit
GAATGCTGCCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT9150.1598965478073203No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACATCCGT7790.13613050354306283No Hit
GAACTGTCCCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT7650.13368399898644812No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT6070.10607344756179608Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCC5900.1031026920287639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT406.8048394E-945.06
GCAAACG207.0303143E-445.01
TCGATCA253.8883423E-545.017
TCGGCGT406.8048394E-945.04
ATCGTAG207.0303143E-445.01
GGTATCG207.0303143E-445.01
CTCGATC253.8883423E-545.016
CGTTTTT67600.043.6686361
GATACCT31600.042.3655055
TACGGCT24450.042.3312877
TGATACC31650.042.227494
CGCGAGG650.041.5384642
ACGGCTG25100.041.32478
CGGCTGT25150.041.153089
GAATCTG32000.041.06251
ATACCTG32900.041.0334366
GGCGACT5100.040.58823410
ATGATAC84600.040.505323
CGTTAGG501.0786607E-940.52
ATACGAA501.0786607E-940.519