##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572245
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.250536046623388	28.0	16.0	31.0	16.0	33.0
2	25.81441340684497	28.0	19.0	31.0	16.0	33.0
3	26.133390418439653	30.0	19.0	31.0	16.0	33.0
4	29.518194130136568	33.0	25.0	35.0	19.0	37.0
5	31.366655890396597	33.0	28.0	35.0	19.0	37.0
6	29.990465622242223	33.0	28.0	35.0	17.0	37.0
7	31.91313161320763	33.0	31.0	35.0	28.0	35.0
8	32.516624872213825	35.0	32.0	35.0	27.0	37.0
9	34.0549974224327	35.0	32.0	37.0	28.0	39.0
10	33.65360291483543	35.0	32.0	39.0	27.0	39.0
11	34.26825048711653	35.0	32.0	39.0	27.0	39.0
12	34.48808989156743	37.0	33.0	39.0	27.0	39.0
13	34.64422930737709	37.0	33.0	39.0	27.0	39.0
14	35.08769495583186	37.0	33.0	39.0	27.0	41.0
15	35.30179206458772	37.0	33.0	40.0	27.0	41.0
16	35.165135562564984	37.0	32.0	40.0	27.0	41.0
17	34.14184658668927	36.0	32.0	39.0	25.0	41.0
18	34.266050380518834	36.0	32.0	39.0	27.0	40.0
19	33.79136558641841	36.0	32.0	39.0	25.0	40.0
20	33.64116768167481	35.0	32.0	38.0	25.0	40.0
21	34.855720888780155	36.0	33.0	39.0	27.0	40.0
22	35.68579891480048	37.0	34.0	39.0	30.0	40.0
23	35.99673741142343	37.0	34.0	40.0	30.0	40.0
24	35.43747695480083	37.0	34.0	39.0	30.0	40.0
25	34.21681971882673	36.0	33.0	39.0	25.0	40.0
26	34.71719455827486	36.0	33.0	39.0	27.0	40.0
27	35.36939073299024	37.0	34.0	39.0	30.0	40.0
28	35.25113019772999	37.0	34.0	39.0	28.0	40.0
29	35.350421585160205	37.0	34.0	40.0	27.0	40.0
30	34.47271535793235	37.0	33.0	39.0	25.0	40.0
31	34.66682452446068	36.0	33.0	39.0	26.0	40.0
32	34.67554980821152	37.0	33.0	39.0	26.0	40.0
33	34.36208442188224	37.0	33.0	39.0	24.0	40.0
34	34.433029559017555	37.0	34.0	40.0	23.0	40.0
35	34.37003206668472	38.0	34.0	40.0	22.0	41.0
36	34.065307691635574	37.0	34.0	40.0	20.0	41.0
37	34.42740259853734	38.0	34.0	40.0	21.0	40.0
38	33.97741526793594	37.0	33.0	40.0	20.0	41.0
39	33.94922629293397	37.0	33.0	40.0	20.0	40.0
40	33.508037641220106	37.0	33.0	40.0	18.0	40.0
41	33.054745781964016	36.0	32.0	39.0	18.0	40.0
42	33.716058681159296	37.0	33.0	40.0	18.0	40.0
43	33.690651731338846	37.0	33.0	40.0	18.0	40.0
44	34.09909042455592	37.0	34.0	40.0	20.0	40.0
45	34.08501603334236	37.0	34.0	40.0	20.0	40.0
46	33.87706314603011	37.0	33.0	40.0	20.0	40.0
47	33.75229840365578	37.0	33.0	40.0	20.0	40.0
48	33.720881790142336	37.0	33.0	40.0	20.0	40.0
49	33.88999117510856	37.0	34.0	40.0	20.0	40.0
50	33.80134208249963	37.0	33.0	40.0	20.0	40.0
51	32.616028099852336	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	8.0
11	10.0
12	19.0
13	21.0
14	40.0
15	66.0
16	112.0
17	241.0
18	516.0
19	933.0
20	1680.0
21	2514.0
22	3568.0
23	5218.0
24	7565.0
25	11439.0
26	14636.0
27	15474.0
28	16053.0
29	17633.0
30	22698.0
31	30052.0
32	39919.0
33	51614.0
34	68074.0
35	92428.0
36	91558.0
37	59688.0
38	17576.0
39	882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.00381829461157	12.80273309509039	16.951655322458038	23.24179328784
2	31.363314664173565	36.2292374769548	18.669625772178	13.737822086693635
3	21.515260072171884	34.86531118664208	30.831025172784383	12.788403568401646
4	19.843947959353077	17.52640914293703	49.013097536894165	13.616545360815735
5	32.08241225349282	19.639140577899326	30.613635767896618	17.664811400711233
6	18.421130809356132	36.64059974311702	32.35991577034312	12.578353677183724
7	59.80952214523499	3.084343244589293	33.706716528759536	3.3994180814161763
8	56.100621237407054	15.44670551948903	22.07481061433477	6.377862628769146
9	51.548550009174384	3.864428697498449	20.86623736336709	23.72078392996007
10	34.434202133701476	16.290574841195642	30.03626069253554	19.238962332567343
11	25.976111630507916	17.615706559253468	35.499130617130774	20.909051193107846
12	20.79022097178656	14.551983853069927	40.98104832720251	23.676746847941004
13	19.132888885005546	17.023302955901755	48.623229560765054	15.220578598327638
14	17.66725790526785	24.86382580887557	35.947015701316744	21.52190058453984
15	13.591730814598643	19.072075771741126	49.04507684645563	18.291116567204607
16	15.50507212819684	21.36130503543063	33.87867084902446	29.254951987348075
17	16.438064115894417	21.072093246773672	39.814240404022755	22.67560223330916
18	16.683238822532307	21.291055404590693	37.80338840881091	24.22231736406609
19	16.846630376849074	23.817071359295404	33.43358177004605	25.902716493809468
20	18.87303515102797	22.0225602670185	42.362274899737	16.742129682216532
21	19.642460834083302	27.144841807267866	35.51031463796102	17.70238272068782
22	17.309194488374736	17.406879920313852	38.01483630263262	27.2690892886788
23	19.056522992774074	26.13443542538598	36.42618109376229	18.38286048807766
24	22.555024508733148	19.557007924927262	31.843878059222885	26.04408950711671
25	16.232382982813306	31.749338133142274	30.212933271588216	21.805345612456204
26	17.144230181128712	20.052425097641745	35.94177319155257	26.861571529676976
27	21.857421209447004	21.974678677839037	30.414245646532517	25.75365446618144
28	15.06277905442599	23.212784733811567	38.56669783047471	23.157738381287736
29	20.686244528130434	22.867827591328886	32.008143365167015	24.437784515373657
30	16.506216742828684	25.897823484696243	33.257957692946206	24.338002079528874
31	20.247621211194506	24.74272383332314	29.72205960733602	25.287595348146336
32	22.75860863790859	24.693793742190845	33.17058253020996	19.377015089690605
33	17.254322886176375	18.98277835542469	29.029524067488577	34.73337469091036
34	22.728027330950905	25.333205183094655	28.061756765021972	23.877010720932468
35	22.981764803536947	19.99598074251413	28.5143601079957	28.507894345953222
36	18.93786752177826	29.550105287071098	31.031638546426795	20.480388644723853
37	18.567921082753017	20.69166178822008	36.98590638625065	23.754510742776258
38	20.061861615217257	25.503761500755793	26.213073071848598	28.22130381217835
39	22.74130835568681	20.74496063748919	32.39521533608856	24.118515670735437
40	23.97644365612631	20.699700303191815	30.89498379190731	24.428872248774564
41	18.24201172574684	25.62870798346862	31.620547143269057	24.50873314751549
42	23.54044159407247	20.532638992040123	35.84286450733514	20.08405490655226
43	23.517898802086517	20.509746699403227	30.936749119695232	25.035605378815017
44	22.410855490218353	19.969942944018733	30.78279408295398	26.836407482808937
45	19.73088449877238	19.468584260238185	30.854266966072224	29.946264274917212
46	26.879745563526114	23.770587772719725	29.31454184833419	20.03512481541997
47	17.032564723151797	19.17517846376989	42.087916888745205	21.70433992433311
48	22.05314157397618	22.549956749294445	28.621482057510335	26.775419619219043
49	18.659315502975122	18.404529528436246	39.114365350505466	23.821789618083162
50	20.785502712998802	18.13716153046335	34.80991533346731	26.267420423070536
51	20.597121862139467	18.48806018401209	30.09689905547449	30.817918898373946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	327.5
2	580.0
3	10913.0
4	21246.0
5	14366.5
6	7487.0
7	7066.0
8	6645.0
9	6548.0
10	6451.0
11	6082.0
12	5713.0
13	5297.0
14	4881.0
15	4544.0
16	4207.0
17	3890.5
18	3574.0
19	3411.5
20	3249.0
21	3032.5
22	2816.0
23	2722.0
24	2628.0
25	2845.5
26	3286.0
27	3509.0
28	3765.0
29	4021.0
30	4687.5
31	5354.0
32	6103.5
33	6853.0
34	7705.5
35	8558.0
36	9506.5
37	10455.0
38	11193.5
39	11932.0
40	13171.0
41	14410.0
42	16075.0
43	17740.0
44	21458.0
45	25176.0
46	76640.0
47	128104.0
48	88303.5
49	48503.0
50	45221.0
51	41939.0
52	35825.5
53	29712.0
54	27356.0
55	25000.0
56	23670.0
57	22340.0
58	21121.0
59	19902.0
60	18899.0
61	17896.0
62	16722.0
63	15548.0
64	14353.0
65	13158.0
66	11350.0
67	9542.0
68	8348.5
69	7155.0
70	6003.0
71	4851.0
72	4075.5
73	3300.0
74	2721.5
75	1714.5
76	1286.0
77	981.5
78	677.0
79	488.0
80	299.0
81	237.5
82	176.0
83	113.5
84	51.0
85	38.5
86	26.0
87	14.0
88	2.0
89	4.0
90	6.0
91	3.0
92	0.0
93	2.0
94	4.0
95	2.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	572245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.097826699342924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83137175564624	30.00575376332008
2	9.069515648858856	7.273357334698422
3	4.590584867217724	5.522174293629673
4	3.147859293199727	5.04889265650555
5	2.317871571688245	4.647080629644442
6	1.6375734131455002	3.9397880956655853
7	1.1990489136903693	3.3655478881634266
8	0.8572453030813779	2.749893888142623
9	0.5738081339830894	2.070761320361454
>10	1.7055103652448131	9.81539973595712
>50	0.03768734019707538	1.0352474915965189
>100	0.022612404116588013	1.8041716544960527
>500	0.0031036633101199232	0.9072416193233502
>1k	0.0035470437829941983	3.8190196403458527
>5k	8.867609457485496E-4	2.453090312252319
>10k+	0.0017735218914970991	15.542579675897558
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	27578	4.819264475880086	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	26098	4.560633994180814	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	21455	3.749268233012084	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12292	2.148031000707739	No Hit
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	7358	1.2858128948265166	No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	6440	1.1253920960427788	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACATCCGTT	4516	0.789172469833725	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTA	4075	0.7121075763003608	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT	3671	0.6415084448094786	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC	3340	0.5836660870780872	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT	2418	0.4225462869924595	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATG	1258	0.21983590944438133	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACATCCGTTCGT	1105	0.1930991096470917	No Hit
GAATCTTTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	1098	0.19187585736878435	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTAT	936	0.1635663046422424	No Hit
GAATGCTGCCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT	915	0.1598965478073203	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACATCCGT	779	0.13613050354306283	No Hit
GAACTGTCCCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	765	0.13368399898644812	No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT	607	0.10607344756179608	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCC	590	0.1031026920287639	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11184020830238796	0.0
2	0.0	0.0	0.0	1.4153028860016252	0.0
3	0.0	0.0	0.0	1.6692151089131404	0.0
4	0.0	0.0	0.0	3.3307412035054917	0.0
5	0.0	0.0	0.0	9.033019073998025	0.0
6	0.0	0.0	0.0	10.00183487841746	0.0
7	0.0	0.0	0.0	11.109751941912991	0.0
8	0.0	0.0	0.0	12.266948597191762	0.0
9	0.0	0.0	0.0	12.596877211683807	0.0
10	0.0	0.0	0.0	17.96450820889654	0.0
11	0.0	0.0	0.0	18.691819063513005	0.0
12	0.0	0.0	0.0	23.335808962944192	0.0
13	0.0	0.0	0.0	23.864952948474865	0.0
14	0.0	0.0	0.0	24.160106248197888	0.0
15	0.0	0.0	0.0	25.126475548060707	0.0
16	0.0	0.0	0.0	25.671172312558433	0.0
17	0.0	0.0	0.0	26.17724925512674	0.0
18	0.0	0.0	0.0	26.6880444564828	0.0
19	0.0	0.0	0.0	27.850658371851218	0.0
20	0.0	0.0	0.0	28.28613618292864	0.0
21	0.0	0.0	0.0	28.628646820854705	0.0
22	0.0	0.0	0.0	29.114103225017256	0.0
23	0.0	0.0	0.0	29.45591486164143	0.0
24	0.0	0.0	0.0	29.750019659411617	0.0
25	0.0	0.0	0.0	29.98907810465797	0.0
26	0.0	0.0	0.0	30.29034766577253	0.0
27	0.0	0.0	0.0	30.58078270670779	0.0
28	1.7475032547248118E-4	0.0	0.0	30.82787966692588	0.0
29	1.7475032547248118E-4	0.0	0.0	31.06449160761562	0.0
30	1.7475032547248118E-4	0.0	0.0	31.3573731531075	0.0
31	1.7475032547248118E-4	0.0	0.0	31.620721893594528	0.0
32	1.7475032547248118E-4	0.0	0.0	31.887740390916477	0.0
33	1.7475032547248118E-4	0.0	0.0	32.161224650280914	0.0
34	1.7475032547248118E-4	0.0	0.0	32.42649564434814	0.0
35	1.7475032547248118E-4	0.0	0.0	32.70959117161356	0.0
36	1.7475032547248118E-4	0.0	0.0	32.989366442695	0.0
37	1.7475032547248118E-4	0.0	0.0	33.25690919099337	0.0
38	3.4950065094496236E-4	0.0	0.0	33.54280072346635	0.0
39	3.4950065094496236E-4	0.0	0.0	33.82240124422232	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	40	6.8048394E-9	45.0	6
GCAAACG	20	7.0303143E-4	45.0	1
TCGATCA	25	3.8883423E-5	45.0	17
TCGGCGT	40	6.8048394E-9	45.0	4
ATCGTAG	20	7.0303143E-4	45.0	1
GGTATCG	20	7.0303143E-4	45.0	1
CTCGATC	25	3.8883423E-5	45.0	16
CGTTTTT	6760	0.0	43.668636	1
GATACCT	3160	0.0	42.365505	5
TACGGCT	2445	0.0	42.331287	7
TGATACC	3165	0.0	42.22749	4
CGCGAGG	65	0.0	41.538464	2
ACGGCTG	2510	0.0	41.3247	8
CGGCTGT	2515	0.0	41.15308	9
GAATCTG	3200	0.0	41.0625	1
ATACCTG	3290	0.0	41.033436	6
GGCGACT	510	0.0	40.588234	10
ATGATAC	8460	0.0	40.50532	3
CGTTAGG	50	1.0786607E-9	40.5	2
ATACGAA	50	1.0786607E-9	40.5	19
>>END_MODULE