SRR3552091_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350543
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	19346	5.5188664443449165	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	16503	4.707838981237679	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	14851	4.236570121211948	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7233	2.0633702570012806	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	4331	1.2355117631788397	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	3826	1.0914495511249689	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTA	3340	0.9528075015048083	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCACTGT	3224	0.9197159834884736	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCT	3051	0.870363978171009	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTC	2343	0.6683916095885526	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATG	939	0.2678701329080883	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGT	935	0.26672904607993886	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	931	0.26558795925178935	No Hit
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	788	0.22479410514544576	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTAT	775	0.22108557295396	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC	647	0.18457079445317692	No Hit
GAATCTTTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	606	0.17287465446464487	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTCACTG	574	0.1637459598394491	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	518	0.1477707442453565	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCCCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	372	0.1061210750179008	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCTGTCAC	355	0.10127145599826556	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
GAATGCTGCCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	354	0.10098618429122817	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	30	2.1611686E-6	45.000004	6
GATCCGT	35	1.2087003E-7	45.000004	5
TTGAGCG	25	3.8852384E-5	45.000004	1
CACAACG	35	1.2087003E-7	45.000004	12
ACCGGTC	20	7.02657E-4	45.0	17
TCCGCTA	20	7.02657E-4	45.0	1
CACGGGC	55	1.8189894E-12	45.0	4
CTAACGG	40	6.7921064E-9	45.0	2
AATTGAG	20	7.02657E-4	45.0	1
CGCTACG	20	7.02657E-4	45.0	3
GAGTTAG	20	7.02657E-4	45.0	1
GCTACGA	20	7.02657E-4	45.0	4
GCTAACG	20	7.02657E-4	45.0	1
CAATAGG	20	7.02657E-4	45.0	2
GTTATAG	20	7.02657E-4	45.0	1
GGCTAAT	20	7.02657E-4	45.0	8
GTAGGGT	65	0.0	44.999996	4
CGTTTTT	4380	0.0	44.17808	1
TGATCCG	85	0.0	42.35294	4
ACGGCTG	1755	0.0	41.666668	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content