##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350543
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.62149864638575	28.0	16.0	31.0	16.0	33.0
2	26.021275563910848	28.0	23.0	31.0	16.0	33.0
3	26.207058763118933	30.0	23.0	31.0	16.0	33.0
4	29.545339658757985	33.0	26.0	35.0	19.0	37.0
5	31.496638072932566	33.0	29.0	35.0	25.0	37.0
6	30.1478049768502	33.0	28.0	35.0	17.0	37.0
7	31.89945313413761	33.0	31.0	35.0	28.0	35.0
8	32.26489760172076	35.0	32.0	35.0	25.0	37.0
9	34.027003819788156	35.0	32.0	37.0	28.0	39.0
10	33.72024830049381	35.0	32.0	39.0	27.0	39.0
11	34.435413059168205	35.0	32.0	39.0	27.0	39.0
12	34.53938318551504	37.0	33.0	39.0	27.0	39.0
13	34.72311528114953	37.0	33.0	39.0	27.0	39.0
14	35.214746835623586	37.0	34.0	39.0	27.0	41.0
15	35.34035767366628	37.0	33.0	40.0	27.0	41.0
16	35.076130460457065	37.0	32.0	40.0	27.0	41.0
17	34.079545162790296	36.0	32.0	39.0	25.0	41.0
18	34.223715778092846	36.0	32.0	39.0	27.0	40.0
19	33.695646468478905	36.0	32.0	38.0	25.0	40.0
20	33.483047728809304	35.0	31.0	38.0	25.0	40.0
21	34.79798198794442	36.0	33.0	39.0	27.0	40.0
22	35.557024387878236	37.0	34.0	39.0	30.0	40.0
23	35.860621949375684	37.0	34.0	40.0	30.0	40.0
24	35.358209977092685	37.0	34.0	39.0	30.0	40.0
25	34.30946845322828	36.0	33.0	39.0	25.0	40.0
26	34.7367626796142	36.0	33.0	39.0	27.0	40.0
27	35.290041449979036	37.0	34.0	39.0	30.0	40.0
28	35.10721937109	37.0	34.0	39.0	27.0	40.0
29	35.4406420895582	38.0	34.0	40.0	27.0	40.0
30	34.44298987570712	37.0	33.0	39.0	25.0	40.0
31	34.57653411992252	36.0	33.0	39.0	25.0	40.0
32	34.474874694402686	37.0	33.0	39.0	25.0	40.0
33	34.17906790322443	37.0	33.0	39.0	23.0	40.0
34	34.379137509520945	38.0	34.0	40.0	22.0	40.0
35	34.34282527393216	38.0	34.0	40.0	20.0	41.0
36	33.980798361399316	38.0	33.0	40.0	18.0	41.0
37	34.24182482605558	38.0	34.0	40.0	18.0	40.0
38	33.806722713048046	37.0	33.0	40.0	18.0	40.0
39	33.869368379913446	37.0	34.0	40.0	18.0	40.0
40	33.20245733048442	37.0	33.0	40.0	16.0	40.0
41	32.99849947082098	36.0	32.0	39.0	16.0	40.0
42	33.55639393740568	37.0	33.0	40.0	17.0	40.0
43	33.61585882473762	37.0	33.0	40.0	17.0	40.0
44	34.00916863266418	38.0	34.0	40.0	18.0	40.0
45	34.059653166658585	38.0	34.0	40.0	18.0	40.0
46	33.82071529027822	37.0	34.0	40.0	18.0	40.0
47	33.6550608627187	37.0	33.0	40.0	19.0	40.0
48	33.624117440656356	37.0	33.0	40.0	18.0	40.0
49	33.774980530205994	37.0	34.0	40.0	18.0	40.0
50	33.67030863545985	37.0	33.0	40.0	18.0	40.0
51	32.286019118909806	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	6.0
12	7.0
13	11.0
14	13.0
15	28.0
16	63.0
17	183.0
18	351.0
19	722.0
20	1200.0
21	1736.0
22	2594.0
23	3701.0
24	5437.0
25	7538.0
26	9051.0
27	9520.0
28	9454.0
29	10470.0
30	13370.0
31	17808.0
32	23671.0
33	29782.0
34	40771.0
35	57002.0
36	57850.0
37	37274.0
38	10452.0
39	469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.05670916264196	13.014380546751752	17.155099374399146	22.77381091620714
2	30.949127496484024	37.9288703525673	18.33155989422125	12.790442256727419
3	19.926228736560137	36.76553233126892	31.232117029865098	12.076121902305852
4	18.779721745976953	16.619073836875934	52.1131501698793	12.48805424726781
5	33.633534259705655	18.99595769991128	30.868680875099493	16.501827165283576
6	16.886373426369946	38.84145454337985	32.58658709487852	11.685584935371693
7	57.06489646063393	2.4573304844198858	36.82144558584824	3.656327469097943
8	52.47829795488713	17.905649235614458	22.937556876046592	6.678495933451817
9	48.92980319104932	3.264934687042674	21.412209058517785	26.393053063390226
10	34.32417706244312	14.817012463520882	30.96167945159367	19.897131022442323
11	25.598571359291157	16.24964697626254	36.743281138120004	21.4085005263263
12	20.16443061193634	12.597313311063122	42.274128994160485	24.96412708284005
13	19.02534068573613	15.642018240272948	50.775511135581084	14.557129938409838
14	16.619073836875934	24.712802708940128	36.464570680344494	22.203552773839444
15	11.721529170458403	17.325121311793417	51.9879158904899	18.96543362725828
16	13.376105071275134	20.241453972836428	34.47537106717293	31.907069888715505
17	14.933118048285088	20.481652750161892	41.85535012822964	22.729879073323385
18	14.981043695067367	20.33217037567431	39.777431014169444	24.909354915088876
19	15.1530625344109	23.1032997378353	34.53156959345929	27.21206813429451
20	17.638634917827485	21.00854959305991	44.41395206864778	16.938863420464823
21	18.55293073888225	28.127219770470386	35.75424412982145	17.56560536082592
22	15.720467959708223	16.800791914258735	38.994645450058904	28.48409467597413
23	17.862858479558856	26.464370990149565	36.841699877047894	18.831070653243682
24	21.76851342060746	18.993675526254982	31.66744165480412	27.570369398333444
25	14.636435472966228	32.29475413857929	30.997908958387416	22.070901430067067
26	16.10444367738052	17.504271943812885	39.28590786294406	27.105376515862535
27	22.712477499194108	21.411353243396672	30.88265918874432	24.9935100686649
28	14.148050310518254	19.227313054318586	43.176443403519684	23.44819323164348
29	22.44546318140713	15.08802058520638	37.58169468510283	24.88482154828366
30	21.44216258775671	25.053987670556822	35.88860710383605	17.61524263785042
31	20.756654675745914	17.03557052915049	34.92752672282716	27.280248072276443
32	23.494121976476496	23.827034058589103	30.399123645315978	22.27972031961842
33	19.77646109036552	22.208687664566114	28.227920683054574	29.78693056201379
34	21.76509016012301	18.023751722327933	35.72999603472327	24.48116208282579
35	24.505124906216928	18.918649067304155	32.698698875744206	23.877527150734718
36	24.82976410882545	19.26154565916307	37.03226137734886	18.876428854662624
37	23.031411267661884	18.141283665627327	33.651506377249014	25.175798689461775
38	20.254576471360146	26.13973178754104	30.558019986135793	23.047671754963016
39	23.441061438967544	19.149719150004422	27.986866090607997	29.422353320420036
40	19.52171345598115	23.507815018414288	36.73757570397925	20.232895821625306
41	24.817212153715808	22.89961573901062	27.098815266600674	25.1843568406729
42	19.82067820495631	18.22315664554705	41.305916820475666	20.650248329020975
43	28.419623270183685	18.59714785347304	28.20481367478455	24.778415201558722
44	24.34223476149859	17.709382301172752	31.88624505410178	26.062137883226878
45	18.95887237799642	16.816767129852828	34.20493348890151	30.019427003249245
46	28.83554941904417	22.92215220386657	28.328621595638765	19.913676781450494
47	16.84814701762694	19.102364046636218	43.57867651044237	20.470812425294472
48	23.44020562384643	21.03565040522846	28.674656176275093	26.849487794650013
49	18.80853418838773	16.500971350162462	40.51371729003289	24.176777171416916
50	22.473419808696793	16.253355508454025	34.072567416836165	27.20065726601301
51	21.532878990594593	16.3149741971741	30.621350305098087	31.530796507133218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	172.5
2	300.0
3	6443.5
4	12587.0
5	8563.5
6	4540.0
7	4340.0
8	4140.0
9	4077.5
10	4015.0
11	3838.0
12	3661.0
13	3393.0
14	3125.0
15	2915.5
16	2706.0
17	2511.0
18	2316.0
19	2205.0
20	2094.0
21	1948.0
22	1802.0
23	1726.0
24	1650.0
25	1700.0
26	1820.0
27	1890.0
28	1935.5
29	1981.0
30	2327.5
31	2674.0
32	2955.0
33	3236.0
34	3885.5
35	4535.0
36	4997.5
37	5460.0
38	5913.0
39	6366.0
40	7153.0
41	7940.0
42	9082.5
43	10225.0
44	12746.0
45	15267.0
46	50600.0
47	85933.0
48	59541.0
49	33149.0
50	30559.5
51	27970.0
52	23081.5
53	18193.0
54	16529.5
55	14866.0
56	13693.0
57	12520.0
58	11809.5
59	11099.0
60	10456.5
61	9814.0
62	9315.5
63	8817.0
64	8077.0
65	7337.0
66	6515.5
67	5694.0
68	4875.0
69	4056.0
70	3373.5
71	2691.0
72	2302.5
73	1914.0
74	1471.0
75	803.0
76	578.0
77	435.0
78	292.0
79	225.5
80	159.0
81	118.5
82	78.0
83	55.0
84	32.0
85	22.0
86	12.0
87	6.5
88	1.0
89	2.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	350543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.42472286561319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6598578729355	31.75613367303649
2	8.94798156326306	7.41335312929577
3	4.357525690269315	5.415278822975887
4	2.8981496256713077	4.802201802660582
5	2.095011174560304	4.339262865326169
6	1.4015448963642314	3.4835165349361707
7	0.9717619397440351	2.8178478331673156
8	0.7147570345797232	2.368688965897003
9	0.4729045326968305	1.7630945287972666
>10	1.4066906058218844	8.393110335456228
>50	0.03760390057606068	1.097335061620701
>100	0.022980161463148192	1.8992781403024956
>500	0.0062673167626767804	1.936490606903198
>1k	0.004178211175117854	5.802548571109463
>5k	6.96368529186309E-4	2.086494348239361
>10k+	0.002089105587558927	14.625364780275902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	19346	5.5188664443449165	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	16503	4.707838981237679	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	14851	4.236570121211948	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7233	2.0633702570012806	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	4331	1.2355117631788397	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	3826	1.0914495511249689	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTA	3340	0.9528075015048083	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCACTGT	3224	0.9197159834884736	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCT	3051	0.870363978171009	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTC	2343	0.6683916095885526	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATG	939	0.2678701329080883	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGT	935	0.26672904607993886	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	931	0.26558795925178935	No Hit
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	788	0.22479410514544576	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTAT	775	0.22108557295396	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC	647	0.18457079445317692	No Hit
GAATCTTTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	606	0.17287465446464487	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTCACTG	574	0.1637459598394491	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	518	0.1477707442453565	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCCCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	372	0.1061210750179008	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCTGTCAC	355	0.10127145599826556	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
GAATGCTGCCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	354	0.10098618429122817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23677551684101522	0.0
2	0.0	0.0	0.0	1.852554465500666	0.0
3	0.0	0.0	0.0	2.186322362734386	0.0
4	0.0	0.0	0.0	3.75303457778361	0.0
5	0.0	0.0	0.0	9.578568107193696	0.0
6	0.0	0.0	0.0	10.498284090682171	0.0
7	0.0	0.0	0.0	11.98740240141723	0.0
8	0.0	0.0	0.0	13.473953266788953	0.0
9	0.0	0.0	0.0	13.910704250263164	0.0
10	0.0	0.0	0.0	20.446564330196296	0.0
11	0.0	0.0	0.0	21.59364186419355	0.0
12	0.0	0.0	0.0	27.072570269553236	0.0
13	0.0	0.0	0.0	27.746382041575497	0.0
14	0.0	0.0	0.0	28.12008797779445	0.0
15	0.0	0.0	0.0	29.378706749243317	0.0
16	0.0	0.0	0.0	30.107290689016754	0.0
17	0.0	0.0	0.0	30.75999235471825	0.0
18	0.0	0.0	0.0	31.450635157455718	0.0
19	0.0	0.0	0.0	32.83933782731363	0.0
20	0.0	0.0	0.0	33.38848586336056	0.0
21	0.0	0.0	0.0	33.84549113803442	0.0
22	0.0	0.0	0.0	34.443420635984744	0.0
23	0.0	0.0	0.0	34.84622428632151	0.0
24	2.8527170703736775E-4	0.0	0.0	35.13691615579258	0.0
25	2.8527170703736775E-4	0.0	0.0	35.40906536430624	0.0
26	2.8527170703736775E-4	0.0	0.0	35.71715880790659	0.0
27	2.8527170703736775E-4	0.0	0.0	36.07916860413701	0.0
28	2.8527170703736775E-4	0.0	0.0	36.32678444584545	0.0
29	2.8527170703736775E-4	0.0	0.0	36.57582664608907	0.0
30	2.8527170703736775E-4	0.0	0.0	36.89304878431462	0.0
31	2.8527170703736775E-4	0.0	0.0	37.16890652501976	0.0
32	2.8527170703736775E-4	0.0	0.0	37.431927038908206	0.0
33	2.8527170703736775E-4	0.0	0.0	37.71577238741039	0.0
34	2.8527170703736775E-4	0.0	0.0	37.97479909740032	0.0
35	2.8527170703736775E-4	0.0	0.0	38.25322428346879	0.0
36	2.8527170703736775E-4	0.0	0.0	38.51168045004464	0.0
37	2.8527170703736775E-4	0.0	0.0	38.768710258085314	0.0
38	2.8527170703736775E-4	0.0	0.0	39.0342982173371	0.0
39	2.8527170703736775E-4	0.0	0.0	39.299315633174814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	30	2.1611686E-6	45.000004	6
GATCCGT	35	1.2087003E-7	45.000004	5
TTGAGCG	25	3.8852384E-5	45.000004	1
CACAACG	35	1.2087003E-7	45.000004	12
ACCGGTC	20	7.02657E-4	45.0	17
TCCGCTA	20	7.02657E-4	45.0	1
CACGGGC	55	1.8189894E-12	45.0	4
CTAACGG	40	6.7921064E-9	45.0	2
AATTGAG	20	7.02657E-4	45.0	1
CGCTACG	20	7.02657E-4	45.0	3
GAGTTAG	20	7.02657E-4	45.0	1
GCTACGA	20	7.02657E-4	45.0	4
GCTAACG	20	7.02657E-4	45.0	1
CAATAGG	20	7.02657E-4	45.0	2
GTTATAG	20	7.02657E-4	45.0	1
GGCTAAT	20	7.02657E-4	45.0	8
GTAGGGT	65	0.0	44.999996	4
CGTTTTT	4380	0.0	44.17808	1
TGATCCG	85	0.0	42.35294	4
ACGGCTG	1755	0.0	41.666668	8
>>END_MODULE