FastQCFastQC Report
Sat 18 Jun 2016
SRR3552089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426689
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC159943.748397544816014RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG140523.2932651181539714No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC117052.7432157847987644No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77961.827091863160288No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT44431.0412736208339095TruSeq Adapter, Index 4 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC33060.7748031938953195RNA PCR Primer, Index 48 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCAATCT26190.6137959966158022No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTA20910.4900524738158237No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT19970.4680223769537063TruSeq Adapter, Index 4 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC18260.4279463496832588No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT14490.33959159950221357TruSeq Adapter, Index 4 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATG6840.16030410908179027No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGT6310.14788288425527726No Hit
GAATCTTTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC6130.14366435506891437RNA PCR Primer, Index 48 (95% over 22bp)
GAATGCTGCCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT5860.13733656128937RNA PCR Primer, Index 48 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTAT5260.123274797334827No Hit
GAACTGTCCCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT4810.11272847436891975RNA PCR Primer, Index 4 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT207.0282957E-445.037
TCGATTT207.0282957E-445.030
CGTTCGA207.0282957E-445.014
TCGATAG207.0282957E-445.01
CACTCGA207.0282957E-445.011
TCGTCGC207.0282957E-445.026
TCGTCCC253.886668E-545.038
GCGATCG207.0282957E-445.09
CTTACTA453.8380676E-1045.038
CGTTTTT49600.043.4122961
TACGGCT13200.042.7840927
ACGGCTG13600.041.5257388
CGCTACT603.6379788E-1241.25000442
CGGCTGT13950.040.9677439
TGATACC17300.040.838154
GATACCT17300.040.708095
AATCTGT19000.040.52
GAATCTG19100.040.287961
CCTAGCG403.451405E-739.3751
CCGTATG403.451405E-739.37530
Produced by FastQC (version 0.11.5)