FastQCFastQC Report
Sat 18 Jun 2016
SRR3552088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470286
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC218344.642706778428446RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG158803.376668665450386No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC120422.560569525777931No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98142.086815257098872No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT51331.091463492428012TruSeq Adapter, Index 4 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC37270.7924964808648355RNA PCR Primer, Index 48 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT27090.576032456845409TruSeq Adapter, Index 4 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC25360.5392463309560566No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT18670.39699246841283814TruSeq Adapter, Index 4 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTA15020.3193801218832795No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCC5500.11695011120892393No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCAATCT5290.11248474332640139No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATG4770.10142764190301222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAT302.1627548E-645.00000410
ATCGTTG207.029032E-445.023
ACGGCTC207.029032E-445.034
GGGCGTA207.029032E-445.07
TGTTCGA207.029032E-445.025
ACATGCG207.029032E-445.01
CAGTACG253.8872782E-545.01
GCGTAAA207.029032E-445.09
AATCGTT207.029032E-445.022
CGTTTTT56000.043.8751
TACGGCT14700.043.1632657
GAATCTG28650.041.5445021
ACGGCTG15350.041.4820868
AATCTGT28900.041.3408282
TGATACC20450.041.2591674
GATACCT20500.041.0487825
GAATGAT47500.040.8315771
ATGATAC45400.040.4900863
AATGATA45100.040.460092
TGATACG24800.040.1008074
Produced by FastQC (version 0.11.5)