##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470286
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.423565660045163	28.0	25.0	31.0	16.0	33.0
2	27.263926631879325	30.0	25.0	31.0	16.0	33.0
3	27.53506802243741	30.0	25.0	33.0	16.0	33.0
4	30.966992425885525	33.0	28.0	35.0	19.0	37.0
5	31.67852540794325	33.0	30.0	35.0	26.0	35.0
6	31.80824647129619	35.0	32.0	35.0	25.0	37.0
7	32.946855743101004	35.0	32.0	35.0	28.0	37.0
8	33.60178912406493	35.0	33.0	35.0	29.0	37.0
9	34.746156594072545	37.0	33.0	39.0	28.0	39.0
10	35.043990252739825	37.0	34.0	39.0	30.0	39.0
11	35.42298090948912	37.0	34.0	39.0	30.0	39.0
12	34.43276644424882	35.0	32.0	38.0	27.0	39.0
13	34.97747115584984	37.0	34.0	39.0	30.0	39.0
14	36.400016160379	38.0	35.0	40.0	31.0	41.0
15	36.18847892558996	38.0	34.0	40.0	30.0	41.0
16	36.50947295900792	38.0	34.0	40.0	31.0	41.0
17	35.058919466027056	37.0	32.0	40.0	27.0	41.0
18	34.82163194311546	37.0	32.0	39.0	27.0	40.0
19	34.55093071024866	37.0	32.0	39.0	27.0	40.0
20	34.8278579417631	36.0	33.0	39.0	27.0	40.0
21	35.76783489195936	37.0	34.0	39.0	30.0	40.0
22	36.380598189187005	38.0	35.0	40.0	31.0	40.0
23	36.449109690698855	38.0	35.0	40.0	31.0	41.0
24	36.542329561160656	38.0	35.0	40.0	31.0	41.0
25	35.90645266922681	38.0	35.0	40.0	30.0	41.0
26	35.91723334311462	38.0	35.0	40.0	30.0	41.0
27	36.45322420824775	38.0	35.0	40.0	31.0	41.0
28	36.41805199389308	38.0	35.0	40.0	31.0	41.0
29	36.455984230872275	38.0	35.0	40.0	31.0	41.0
30	36.12836231569726	38.0	35.0	40.0	30.0	41.0
31	36.02626486861187	38.0	35.0	40.0	30.0	41.0
32	35.747075184036945	38.0	35.0	40.0	30.0	41.0
33	35.1448735450343	38.0	35.0	40.0	25.0	41.0
34	34.65129091659118	38.0	34.0	40.0	23.0	41.0
35	34.59065334711218	38.0	34.0	40.0	21.0	41.0
36	34.56952577793088	38.0	34.0	40.0	21.0	41.0
37	34.386054443466314	38.0	34.0	40.0	19.0	41.0
38	34.2791599154557	38.0	34.0	40.0	18.0	41.0
39	33.64064845647117	37.0	33.0	40.0	18.0	41.0
40	33.270709738329444	37.0	33.0	40.0	15.0	41.0
41	33.15235409942035	37.0	33.0	40.0	15.0	41.0
42	33.43418260377727	37.0	33.0	40.0	15.0	41.0
43	33.49015280063621	37.0	33.0	40.0	15.0	41.0
44	33.34178563682525	37.0	33.0	40.0	15.0	41.0
45	33.37267535074402	37.0	33.0	40.0	15.0	41.0
46	32.8896947814734	36.0	32.0	40.0	14.0	40.0
47	32.73357488847212	36.0	32.0	39.0	12.0	40.0
48	32.88536550099301	36.0	32.0	39.0	15.0	40.0
49	32.806173264779304	36.0	32.0	39.0	13.0	40.0
50	32.78362740970388	36.0	32.0	39.0	15.0	40.0
51	29.791484330811464	34.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	8.0
11	11.0
12	16.0
13	24.0
14	42.0
15	88.0
16	212.0
17	414.0
18	650.0
19	923.0
20	1386.0
21	1964.0
22	2783.0
23	4071.0
24	6192.0
25	9232.0
26	12179.0
27	13425.0
28	13036.0
29	13420.0
30	15038.0
31	18597.0
32	24712.0
33	32756.0
34	42463.0
35	61407.0
36	76991.0
37	75229.0
38	39366.0
39	3642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.26178963439269	13.938964800142891	17.3855908957528	24.413654669711622
2	32.162981674980756	34.98488154016917	20.216634133272095	12.635502651577976
3	23.543758478883063	33.2151074027294	31.394725762621047	11.846408355766492
4	21.516906733349494	18.531064075902748	45.95543988126374	13.996589309484017
5	28.224314566030035	22.166298805407774	31.06577699527521	18.543609633286977
6	19.02416827207274	36.324066631794274	33.57744011091123	11.07432498522176
7	64.6015403392829	3.669894489736033	28.290231901438702	3.4383332695423636
8	60.069191938522515	11.684591929166507	22.721918151933078	5.524297980377898
9	55.456041642745056	4.624632670332521	20.95852311146834	18.960802575454085
10	32.65204577639989	17.777692723151446	30.898857291095204	18.671404209353458
11	23.447221477994244	18.754119833463044	35.54709262023535	22.251566068307373
12	21.142666377480936	15.925840871299593	42.24429389775584	20.687198853463638
13	17.9139927618513	17.874654997171934	48.34568751780831	15.865664723168454
14	17.31371973650077	25.127475621217727	36.680870789264404	20.8779338530171
15	14.431218450049544	19.95253951850576	48.71099713791182	16.905244893532874
16	16.411502787665377	23.275198496234207	34.42181991383966	25.891478802260753
17	17.281186341928105	20.283189378378264	38.80787435730598	23.627749922387654
18	17.78088227163896	22.823133157270256	37.179928809277754	22.216055761813024
19	17.878057182225284	23.50548389703287	33.64144371722739	24.97501520351446
20	19.113688266289024	23.47656532407939	39.84426497918288	17.565481430448706
21	19.982733910854247	25.826199376549592	36.609424903144046	17.58164180945212
22	18.15235835215167	18.44749790552983	35.536460791943625	27.86368295037488
23	19.445188672424866	25.169365024687107	36.82503838090013	18.560407921987895
24	23.675167876568725	19.779878627048223	31.545059814665972	24.999893681717083
25	17.557188604381164	30.98008445924395	30.61583802196961	20.846888914405277
26	19.325474285860096	19.595310087903954	34.68357552638182	26.395640099854134
27	23.48613396954194	22.85949401002794	30.22990265498016	23.42446936544996
28	18.92571754209141	19.823469123044273	37.381083000557105	23.86973033430721
29	27.412893430805934	19.602965004273994	29.966020676779664	23.01812088814041
30	20.039295237366197	29.345547177674863	32.45216740451555	18.16299018044339
31	27.579175225288445	21.216025992693808	28.310007102061295	22.89479167995645
32	23.682822792938765	24.47085390592108	28.976197462820497	22.87012583831966
33	19.301446353920806	27.61340971238778	27.605754796017738	25.47938913767367
34	26.645275428143727	26.403082379658336	28.552412787112523	18.399229405085414
35	19.096252067890603	25.567420675929114	32.145545476582335	23.19078177959795
36	27.244485270665088	22.881608212874717	26.360555066491454	23.51335144996874
37	19.261895952675605	25.443878831179322	31.820849440553197	23.473375775591872
38	22.537987522486315	26.647189157236234	26.15663659985626	24.65818672042119
39	24.681364106097142	25.24527627868999	27.44266255002275	22.63069706519012
40	20.129878414411657	25.457912844524394	35.53518497254862	18.87702376851533
41	18.585924309888025	30.12273382579962	27.201107411234865	24.09023445307749
42	20.252569712898108	21.704664820981275	37.97455165580094	20.068213810319676
43	25.25973556516673	21.203055162177908	26.827504965063813	26.709704307591554
44	22.75870427782243	21.687228622582854	29.718724350714247	25.835342748880468
45	20.438839344569047	20.36037645177615	29.99387606690397	29.20690813675083
46	27.247036909455097	24.31265230094028	27.99530498462638	20.445005804978248
47	19.00630680054265	20.764811199993197	39.70328693603467	20.52559506342949
48	22.421675320974895	22.95262882586341	28.628324041115405	25.99737181204629
49	18.260803000727215	20.537077437984546	37.930323250107385	23.271796311180857
50	20.664446740919356	19.722679390838767	34.50815035956843	25.104723508673445
51	21.120126901502488	19.46560178274497	29.5720051202885	29.842266195464035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	351.5
2	599.0
3	8390.5
4	16182.0
5	11276.0
6	6370.0
7	5996.5
8	5623.0
9	5563.5
10	5504.0
11	5302.0
12	5100.0
13	4725.0
14	4350.0
15	4031.0
16	3712.0
17	3501.5
18	3291.0
19	3049.5
20	2808.0
21	2705.0
22	2602.0
23	2541.0
24	2480.0
25	2592.0
26	2948.5
27	3193.0
28	3655.0
29	4117.0
30	4536.5
31	4956.0
32	5709.0
33	6462.0
34	7133.0
35	7804.0
36	8558.0
37	9312.0
38	10192.0
39	11072.0
40	11979.0
41	12886.0
42	14683.5
43	16481.0
44	19024.0
45	21567.0
46	56808.5
47	92050.0
48	63941.5
49	35833.0
50	34255.5
51	32678.0
52	28821.5
53	24965.0
54	23035.0
55	21105.0
56	20004.5
57	18904.0
58	18368.5
59	17833.0
60	17056.5
61	16280.0
62	15287.5
63	14295.0
64	12872.0
65	11449.0
66	9945.5
67	8442.0
68	7351.0
69	6260.0
70	5278.0
71	4296.0
72	3507.0
73	2718.0
74	2262.5
75	1399.0
76	991.0
77	765.5
78	540.0
79	395.5
80	251.0
81	203.5
82	156.0
83	114.5
84	73.0
85	50.5
86	28.0
87	25.5
88	23.0
89	15.5
90	8.0
91	10.0
92	12.0
93	9.0
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.908810741952784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.20022806380116	30.354430868717753
2	8.803595855480038	7.202892734009455
3	4.4541288690405505	5.4663934477154426
4	3.0884455582089756	5.053785393103828
5	2.3106578609495094	4.726313256149447
6	1.6969866736410502	4.165302399815864
7	1.3030332937029105	3.731387968178902
8	0.9884143424740554	3.2347884216722265
9	0.7508178507618906	2.7643558822648138
>10	2.3325139132026265	13.337816266959463
>50	0.038775921401632225	1.0475946023640212
>100	0.02602767326958718	1.938951801886271
>500	0.0010623540110035583	0.23446475336044906
>1k	0.0026558850275088955	2.681677035422893
>5k	0.0010623540110035583	3.2479561339004928
>10k+	0.0015935310165053373	10.811889034478686
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	21834	4.642706778428446	RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG	15880	3.376668665450386	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC	12042	2.560569525777931	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9814	2.086815257098872	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	5133	1.091463492428012	TruSeq Adapter, Index 4 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	3727	0.7924964808648355	RNA PCR Primer, Index 48 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT	2709	0.576032456845409	TruSeq Adapter, Index 4 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC	2536	0.5392463309560566	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT	1867	0.39699246841283814	TruSeq Adapter, Index 4 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTA	1502	0.3193801218832795	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCC	550	0.11695011120892393	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCAATCT	529	0.11248474332640139	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATG	477	0.10142764190301222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1263656583440715E-4	0.0	0.0	0.11992702313060563	0.0
2	2.1263656583440715E-4	0.0	0.0	1.1765181187617748	0.0
3	2.1263656583440715E-4	0.0	0.0	1.4201996232080054	0.0
4	2.1263656583440715E-4	0.0	0.0	2.8863287446362427	0.0
5	2.1263656583440715E-4	0.0	0.0	9.107436751253493	0.0
6	2.1263656583440715E-4	0.0	0.0	10.06408866094249	0.0
7	2.1263656583440715E-4	0.0	0.0	11.134288496787061	0.0
8	2.1263656583440715E-4	0.0	0.0	12.280186950068682	0.0
9	2.1263656583440715E-4	0.0	0.0	12.596164886898611	0.0
10	2.1263656583440715E-4	0.0	0.0	16.99497752431499	0.0
11	2.1263656583440715E-4	0.0	0.0	17.70412047137274	0.0
12	2.1263656583440715E-4	0.0	0.0	21.192635970452024	0.0
13	2.1263656583440715E-4	0.0	0.0	21.574531242690618	0.0
14	2.1263656583440715E-4	0.0	0.0	21.81247155985932	0.0
15	2.1263656583440715E-4	0.0	0.0	22.335344875246125	0.0
16	2.1263656583440715E-4	0.0	0.0	22.74764717639904	0.0
17	2.1263656583440715E-4	0.0	0.0	23.147616556733563	0.0
18	2.1263656583440715E-4	0.0	0.0	23.580544604772417	0.0
19	2.1263656583440715E-4	0.0	0.0	23.98944472087198	0.0
20	2.1263656583440715E-4	0.0	0.0	24.27459035565592	0.0
21	2.1263656583440715E-4	0.0	0.0	24.573132094087427	0.0
22	2.1263656583440715E-4	0.0	0.0	24.92844779559672	0.0
23	2.1263656583440715E-4	0.0	0.0	25.222311529579873	0.0
24	2.1263656583440715E-4	0.0	0.0	25.4585507542219	0.0
25	2.1263656583440715E-4	0.0	0.0	25.678204326728842	0.0
26	2.1263656583440715E-4	0.0	0.0	25.89764526266995	0.0
27	2.1263656583440715E-4	0.0	0.0	26.143027859642856	0.0
28	2.1263656583440715E-4	0.0	0.0	26.358216064267275	0.0
29	2.1263656583440715E-4	0.0	0.0	26.566174625653325	0.0
30	2.1263656583440715E-4	0.0	0.0	26.815172044245415	0.0
31	2.1263656583440715E-4	0.0	0.0	27.054600817374958	0.0
32	2.1263656583440715E-4	0.0	0.0	27.287437856963635	0.0
33	2.1263656583440715E-4	0.0	0.0	27.516660074933124	0.0
34	2.1263656583440715E-4	0.0	0.0	27.74694547573179	0.0
35	2.1263656583440715E-4	0.0	0.0	27.98637424886133	0.0
36	2.1263656583440715E-4	0.0	0.0	28.20879209672412	0.0
37	2.1263656583440715E-4	0.0	0.0	28.440565953483624	0.0
38	2.1263656583440715E-4	0.0	0.0	28.67382826620397	0.0
39	2.1263656583440715E-4	0.0	0.0	28.908791671450988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGAT	30	2.1627548E-6	45.000004	10
ATCGTTG	20	7.029032E-4	45.0	23
ACGGCTC	20	7.029032E-4	45.0	34
GGGCGTA	20	7.029032E-4	45.0	7
TGTTCGA	20	7.029032E-4	45.0	25
ACATGCG	20	7.029032E-4	45.0	1
CAGTACG	25	3.8872782E-5	45.0	1
GCGTAAA	20	7.029032E-4	45.0	9
AATCGTT	20	7.029032E-4	45.0	22
CGTTTTT	5600	0.0	43.875	1
TACGGCT	1470	0.0	43.163265	7
GAATCTG	2865	0.0	41.544502	1
ACGGCTG	1535	0.0	41.482086	8
AATCTGT	2890	0.0	41.340828	2
TGATACC	2045	0.0	41.259167	4
GATACCT	2050	0.0	41.048782	5
GAATGAT	4750	0.0	40.831577	1
ATGATAC	4540	0.0	40.490086	3
AATGATA	4510	0.0	40.46009	2
TGATACG	2480	0.0	40.100807	4
>>END_MODULE