FastQCFastQC Report
Sat 18 Jun 2016
SRR3552087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454472
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG194454.278591420373532No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC189624.17231424598215No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC146893.232102307732929No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91362.01024485556866No Hit
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT46251.0176644545758595No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC44630.9820186942209861TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT33970.7474607896636096No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA28610.6295217307116829No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT26640.5861747258356951No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC22310.49089932933162No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT14440.31773134538541425No Hit
GAATCTTTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC7710.1696474150222676No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGT7590.16700698832931402No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATG7510.16524670386734497No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTAT6770.1489640725941312No Hit
GAATGCTGCCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT6370.14016265028428593No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCACTTTG5850.12872080128148708No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT5330.11727895227868824TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC5250.11551866781671918No Hit
GAACTGTCCCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT4610.10143639212096675TruSeq Adapter, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC207.028778E-445.00000412
CAGTGCG207.028778E-445.0000041
CATACGA207.028778E-445.00000418
GCTAGCG406.7993824E-945.0000041
ATAACGC207.028778E-445.00000411
GTCTTCC207.028778E-445.0000049
TCGTTAG253.8870705E-545.01
CGGTTAT253.8870705E-545.031
CGCTACG253.8870705E-545.03
TATAGCG253.8870705E-545.01
ATACGCA253.8870705E-545.019
TGTAACG253.8870705E-545.01
AGGGCGT302.1625947E-644.9999966
AATACGC302.1625947E-644.99999618
CGTTTTT54800.043.7682461
ACGGCTG18000.042.258
TACGGCT17900.042.234647
CTAGCGG800.042.1875042
TGATACC21500.042.0697674
GATACCT21550.041.9721575
Produced by FastQC (version 0.11.5)