##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	454472
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.29393229945959	28.0	16.0	31.0	16.0	33.0
2	25.843290675773204	28.0	19.0	31.0	16.0	33.0
3	26.232522575648225	30.0	23.0	31.0	16.0	33.0
4	29.52706877431393	33.0	25.0	35.0	19.0	37.0
5	31.360772500836134	33.0	28.0	35.0	19.0	37.0
6	29.839640285870196	33.0	28.0	35.0	17.0	37.0
7	31.799138340755867	33.0	31.0	35.0	28.0	35.0
8	32.477059092749386	35.0	32.0	35.0	27.0	37.0
9	33.952019046277876	35.0	32.0	37.0	28.0	39.0
10	33.52508845429421	35.0	31.0	38.0	27.0	39.0
11	34.17796475910507	35.0	32.0	39.0	27.0	39.0
12	34.40930794416378	37.0	32.0	39.0	27.0	39.0
13	34.60237814430812	37.0	33.0	39.0	27.0	39.0
14	35.038981499410305	37.0	33.0	39.0	27.0	41.0
15	35.253771409459766	37.0	33.0	40.0	27.0	41.0
16	35.09439085356193	37.0	32.0	40.0	27.0	41.0
17	34.04310276540689	36.0	32.0	39.0	25.0	41.0
18	34.20815583798342	36.0	32.0	39.0	26.0	40.0
19	33.734271858332306	36.0	32.0	39.0	25.0	40.0
20	33.595464627083736	35.0	31.0	38.0	25.0	40.0
21	34.75390123043884	36.0	33.0	39.0	27.0	40.0
22	35.51868101885265	37.0	34.0	39.0	30.0	40.0
23	35.88515684134556	37.0	34.0	40.0	30.0	40.0
24	35.28347400939992	37.0	34.0	39.0	29.0	40.0
25	34.085457409917446	36.0	32.0	39.0	25.0	40.0
26	34.59583648717633	36.0	33.0	39.0	27.0	40.0
27	35.159604992166734	37.0	34.0	39.0	29.0	40.0
28	34.99401283247373	37.0	34.0	39.0	27.0	40.0
29	35.19163556830784	37.0	34.0	40.0	27.0	40.0
30	34.269812001619464	36.0	33.0	39.0	24.0	40.0
31	34.397100371420024	36.0	33.0	39.0	25.0	40.0
32	34.32064901688113	37.0	33.0	39.0	25.0	40.0
33	34.12821031878752	37.0	33.0	40.0	23.0	40.0
34	34.33723309686845	38.0	34.0	40.0	22.0	40.0
35	34.22291802355261	38.0	34.0	40.0	21.0	41.0
36	33.942990987343556	37.0	33.0	40.0	18.0	41.0
37	34.262148163143166	38.0	34.0	40.0	20.0	41.0
38	33.64556232287138	37.0	33.0	40.0	18.0	41.0
39	33.70384314105159	37.0	33.0	40.0	18.0	40.0
40	33.21004374306888	37.0	32.0	40.0	17.0	40.0
41	32.83867432977169	36.0	31.0	39.0	15.0	40.0
42	33.54140409090109	37.0	33.0	40.0	18.0	40.0
43	33.571989033427805	37.0	33.0	40.0	18.0	40.0
44	33.95100688271225	37.0	34.0	40.0	18.0	40.0
45	33.937982978049256	37.0	34.0	40.0	19.0	40.0
46	33.69563361439209	37.0	33.0	40.0	18.0	40.0
47	33.55279093101445	37.0	33.0	40.0	20.0	40.0
48	33.49759941206499	37.0	33.0	40.0	18.0	40.0
49	33.6813687091834	37.0	33.0	40.0	20.0	40.0
50	33.554683236811066	37.0	33.0	40.0	18.0	40.0
51	32.3964974739918	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	7.0
11	15.0
12	14.0
13	19.0
14	32.0
15	51.0
16	104.0
17	220.0
18	434.0
19	917.0
20	1565.0
21	2437.0
22	3438.0
23	4797.0
24	6855.0
25	9700.0
26	11991.0
27	13032.0
28	13332.0
29	14717.0
30	18528.0
31	24259.0
32	31279.0
33	39393.0
34	52646.0
35	71180.0
36	71611.0
37	47312.0
38	13916.0
39	659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.453845341407174	13.085074548046965	17.86578711119717	23.595292999348693
2	31.53659631396434	34.91238184090549	19.57546339488461	13.97555845024556
3	22.371675262722455	33.19280395711947	31.18541956380151	13.250101216356564
4	20.287058388635604	17.841583199845097	47.96533119752152	13.906027213997781
5	31.553319016353043	20.29167913534827	30.834902920311926	17.320098927986763
6	18.239627523807847	37.004479923955714	32.00835254977205	12.747540002464397
7	60.65368163495221	3.4215529229523494	32.53797813726698	3.3867873048284607
8	57.57230368427538	15.101700434790263	21.363692372687424	5.962303508246933
9	53.22968191659773	3.9945255153232764	20.181661356475207	22.594131211603795
10	34.95462866799275	17.005448080409792	29.17869527715679	18.86122797444067
11	26.11536024221514	17.563898325969475	35.141438856519216	21.17930257529617
12	20.815363762784067	14.81059339189213	40.697336689609045	23.676706155714765
13	19.596806843985988	16.955940079916914	48.247636818109804	15.19961625798729
14	17.650812371279198	24.770282877713036	36.09089228819377	21.48801246281399
15	13.814492421975391	18.702142266190215	48.196588568712706	19.28677674312169
16	15.659490573676708	20.840887887482616	33.96424862257741	29.53537291626327
17	16.933276417469063	21.317044834445245	39.083815944656656	22.665862803429036
18	17.199299406784135	20.981930679997888	37.53344540477741	24.285324508440564
19	17.260029220722068	23.08767096762837	33.52461757820064	26.127682233448922
20	19.809141157211005	21.526298649861815	41.62038585435406	17.04417433857311
21	20.16735904522171	26.961616997306763	35.073667904733405	17.797356052738124
22	17.788114559312785	17.666874966994666	37.39592318118608	27.14908729250647
23	19.45334366033551	25.582214085796263	35.90628245524477	19.058159798623457
24	22.227772007956485	20.097387737858437	31.874130859546902	25.800709394638172
25	16.584079987325953	31.003890228660953	30.366667253428155	22.045362530584942
26	17.506909116513228	18.82140153848862	35.90298192187858	27.768707423119576
27	22.08584907321023	21.67636290024468	30.442799556408318	25.794988470136776
28	15.44935661602915	21.828627506205002	38.00388142723864	24.718134450527206
29	20.89589677691915	17.441558555862628	31.272113573553483	30.390431093664738
30	17.10248376137584	24.787445651217237	33.9789910049464	24.131079582460526
31	20.63185410762379	23.087891003186115	29.283652238201697	26.9966026509884
32	22.148119136052387	23.42432537097995	30.23266559876076	24.194889894206902
33	19.133191923814888	19.000070411378477	33.82474607896636	28.04199158584027
34	21.325626221197346	19.793078561495538	34.8036842753789	24.077610941928214
35	19.433540460138357	18.92503828618705	33.52923832491331	28.112182928761285
36	23.913244380291857	25.015622524599973	27.930213522505237	23.140919572602932
37	18.912056188280026	20.29872027319615	33.03943917337042	27.749784365153406
38	20.391795314122763	25.12410005456882	30.972205108345506	23.511899522962914
39	23.480434438205215	20.410938407646675	32.23036842753789	23.87825872661022
40	19.321542361245577	20.408297980953723	39.49968314879684	20.770476509003856
41	23.198568888732417	24.30050696192505	31.048337411325672	21.452586738016862
42	19.74797127215758	20.54956080902674	38.397965111161966	21.304502807653716
43	27.074715273988275	19.44784277139186	27.678052773328172	25.799389181291698
44	22.889418930099104	19.791538312591314	31.234707528736642	26.084335228572936
45	19.519574363217096	19.375671108451126	32.22464750303649	28.88010702529529
46	27.367802636906124	23.610035381717687	28.31175517963703	20.71040680173916
47	18.0147511837913	19.471166540512947	41.95285958210847	20.561222693587283
48	22.38971817845764	22.25065570596208	29.887869879772573	25.471756235807707
49	18.811499938390046	18.822721751835097	38.943213223256876	23.42256508651798
50	21.971870654297735	18.33776338256262	33.91474062208453	25.775625341055115
51	21.548082170078683	18.383750814131563	30.158513615800313	29.909653399989438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	280.0
2	490.0
3	8139.0
4	15788.0
5	10868.5
6	5949.0
7	5641.0
8	5333.0
9	5196.5
10	5060.0
11	4799.0
12	4538.0
13	4286.5
14	4035.0
15	3715.0
16	3395.0
17	3194.0
18	2993.0
19	2802.0
20	2611.0
21	2417.5
22	2224.0
23	2245.0
24	2266.0
25	2333.0
26	2635.5
27	2871.0
28	3171.5
29	3472.0
30	3929.0
31	4386.0
32	4708.0
33	5030.0
34	5840.0
35	6650.0
36	7252.0
37	7854.0
38	8597.5
39	9341.0
40	10463.5
41	11586.0
42	12966.5
43	14347.0
44	17252.0
45	20157.0
46	56558.0
47	92959.0
48	65548.5
49	38138.0
50	35981.0
51	33824.0
52	28994.5
53	24165.0
54	22497.5
55	20830.0
56	19860.0
57	18890.0
58	17752.5
59	16615.0
60	15985.5
61	15356.0
62	14282.0
63	13208.0
64	12139.0
65	11070.0
66	9912.0
67	8754.0
68	7695.0
69	6636.0
70	5610.5
71	4585.0
72	3774.5
73	2964.0
74	2350.0
75	1386.0
76	1036.0
77	755.5
78	475.0
79	349.5
80	224.0
81	155.0
82	86.0
83	56.5
84	27.0
85	22.0
86	17.0
87	19.5
88	22.0
89	14.5
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	454472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.35716452063363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4584986158502	30.793923772150883
2	9.136454015855314	7.557156637378641
3	4.593796076332459	5.699591403093683
4	3.29182621118332	5.445623927569706
5	2.3594751155373945	4.879060026781054
6	1.6857657926842393	4.183109593877908
7	1.2290595366496053	3.5581292224010035
8	0.8758251601913267	2.8977316193074403
9	0.6119832237761106	2.277890178261872
>10	1.689495119290882	9.94778630524842
>50	0.0347244199163533	0.997867574238834
>100	0.022787900570106594	1.7565341513698058
>500	0.004340552489544114	1.1753609970458667
>1k	0.0037979834283510994	4.8659227225925195
>5k	5.425690611930142E-4	2.050037813862359
>10k+	0.0016277071835790425	11.914274054820034
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	19445	4.278591420373532	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	18962	4.17231424598215	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	14689	3.232102307732929	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9136	2.01024485556866	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	4625	1.0176644545758595	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	4463	0.9820186942209861	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT	3397	0.7474607896636096	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA	2861	0.6295217307116829	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT	2664	0.5861747258356951	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC	2231	0.49089932933162	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT	1444	0.31773134538541425	No Hit
GAATCTTTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	771	0.1696474150222676	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGT	759	0.16700698832931402	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATG	751	0.16524670386734497	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTAT	677	0.1489640725941312	No Hit
GAATGCTGCCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT	637	0.14016265028428593	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCACTTTG	585	0.12872080128148708	No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	533	0.11727895227868824	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	525	0.11551866781671918	No Hit
GAACTGTCCCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	461	0.10143639212096675	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2003555774613177E-4	0.0	0.0	0.12410005456881833	0.0
2	2.2003555774613177E-4	0.0	0.0	1.3261543065359362	0.0
3	2.2003555774613177E-4	0.0	0.0	1.5866764069073562	0.0
4	2.2003555774613177E-4	0.0	0.0	2.973560527381225	0.0
5	2.2003555774613177E-4	0.0	0.0	7.993671777359221	0.0
6	2.2003555774613177E-4	0.0	0.0	8.828266647890299	0.0
7	2.2003555774613177E-4	0.0	0.0	9.879596542801316	0.0
8	2.2003555774613177E-4	0.0	0.0	10.970092766991145	0.0
9	2.2003555774613177E-4	0.0	0.0	11.310487774824411	0.0
10	2.2003555774613177E-4	0.0	0.0	16.400130261050187	0.0
11	2.2003555774613177E-4	0.0	0.0	17.13592916615325	0.0
12	2.2003555774613177E-4	0.0	0.0	21.33442764350719	0.0
13	2.2003555774613177E-4	0.0	0.0	21.80090302592899	0.0
14	2.2003555774613177E-4	0.0	0.0	22.071766797514478	0.0
15	2.2003555774613177E-4	0.0	0.0	22.969511873118694	0.0
16	2.2003555774613177E-4	0.0	0.0	23.457550740199615	0.0
17	4.4007111549226353E-4	0.0	0.0	23.929086940449576	0.0
18	4.4007111549226353E-4	0.0	0.0	24.409864634124876	0.0
19	4.4007111549226353E-4	0.0	0.0	25.526325054128748	0.0
20	4.4007111549226353E-4	0.0	0.0	25.941752187153444	0.0
21	4.4007111549226353E-4	0.0	0.0	26.236159763417767	0.0
22	4.4007111549226353E-4	0.0	0.0	26.686352514566355	0.0
23	4.4007111549226353E-4	0.0	0.0	26.99770282877713	0.0
24	4.4007111549226353E-4	0.0	0.0	27.25382421799363	0.0
25	4.4007111549226353E-4	0.0	0.0	27.46417821119893	0.0
26	4.4007111549226353E-4	0.0	0.0	27.714798711471772	0.0
27	4.4007111549226353E-4	0.0	0.0	28.02218838564312	0.0
28	4.4007111549226353E-4	0.0	0.0	28.254986005738527	0.0
29	4.4007111549226353E-4	0.0	0.0	28.458738932211446	0.0
30	4.4007111549226353E-4	0.0	0.0	28.69505712123079	0.0
31	4.4007111549226353E-4	0.0	0.0	28.920153496805085	0.0
32	4.4007111549226353E-4	0.0	0.0	29.145249872379377	0.0
33	4.4007111549226353E-4	0.0	0.0	29.386408843669138	0.0
34	4.4007111549226353E-4	0.0	0.0	29.6018236547026	0.0
35	4.4007111549226353E-4	0.0	0.0	29.856844866130366	0.0
36	4.4007111549226353E-4	0.0	0.0	30.085021739513106	0.0
37	4.4007111549226353E-4	0.0	0.0	30.312538506222605	0.0
38	4.4007111549226353E-4	0.0	0.0	30.554137548627857	0.0
39	4.4007111549226353E-4	0.0	0.0	30.784514777588058	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	20	7.028778E-4	45.000004	12
CAGTGCG	20	7.028778E-4	45.000004	1
CATACGA	20	7.028778E-4	45.000004	18
GCTAGCG	40	6.7993824E-9	45.000004	1
ATAACGC	20	7.028778E-4	45.000004	11
GTCTTCC	20	7.028778E-4	45.000004	9
TCGTTAG	25	3.8870705E-5	45.0	1
CGGTTAT	25	3.8870705E-5	45.0	31
CGCTACG	25	3.8870705E-5	45.0	3
TATAGCG	25	3.8870705E-5	45.0	1
ATACGCA	25	3.8870705E-5	45.0	19
TGTAACG	25	3.8870705E-5	45.0	1
AGGGCGT	30	2.1625947E-6	44.999996	6
AATACGC	30	2.1625947E-6	44.999996	18
CGTTTTT	5480	0.0	43.768246	1
ACGGCTG	1800	0.0	42.25	8
TACGGCT	1790	0.0	42.23464	7
CTAGCGG	80	0.0	42.187504	2
TGATACC	2150	0.0	42.069767	4
GATACCT	2155	0.0	41.972157	5
>>END_MODULE