SRR3552086_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503610
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	25409	5.045372411191199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	21640	4.29697583447509	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	14314	2.8422787474434585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11105	2.205079327257203	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	5112	1.0150711860368142	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	5008	0.9944202855384128	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT	3612	0.7172216596175612	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC	3079	0.6113857945632534	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA	1917	0.38065169476380534	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT	1824	0.36218502412581166	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCCTTTGTCGTATGCCGTCTTC	653	0.12966382716784813	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT	641	0.1272810309564941	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCCTTTGTCGTATGC	632	0.1254939337979786	No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	581	0.11536704989972399	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATG	559	0.11099859017890829	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCC	555	0.1102043247751236	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGT	532	0.10563729870336172	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTAT	530	0.10524016600146939	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAAA	30	2.1630622E-6	45.000004	21
CTAGACG	20	7.0295075E-4	45.0	1
CGCGGAT	20	7.0295075E-4	45.0	23
GCGCGAC	20	7.0295075E-4	45.0	9
CGACCCT	25	3.8876737E-5	45.0	12
CGATTTA	25	3.8876737E-5	45.0	10
ACTACCG	25	3.8876737E-5	45.0	26
GACGCTA	50	2.1827873E-11	45.0	40
TATCGCG	25	3.8876737E-5	45.0	1
AATACGC	25	3.8876737E-5	45.0	18
ACGAAAT	20	7.0295075E-4	45.0	36
CGTTTTT	6235	0.0	43.59262	1
GAATGAC	390	0.0	42.692307	1
TACGGCT	1815	0.0	42.272728	7
ATTGGGC	80	0.0	42.1875	4
TGATACC	2795	0.0	41.940968	4
GATACCT	2795	0.0	41.940968	5
ACGGCTG	1835	0.0	41.934605	8
GAATCTG	3520	0.0	41.484375	1
ACGGGAC	55	6.002665E-11	40.909092	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content