##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503610
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.575961557554457	28.0	25.0	31.0	16.0	33.0
2	27.37455967911678	30.0	25.0	31.0	16.0	33.0
3	27.629870336172832	30.0	25.0	31.0	16.0	33.0
4	30.993040249399336	33.0	28.0	35.0	19.0	37.0
5	31.706467306050317	33.0	30.0	35.0	26.0	35.0
6	31.89487698814559	35.0	32.0	35.0	25.0	36.0
7	32.930605031671334	35.0	32.0	35.0	28.0	36.0
8	33.472236452810705	35.0	33.0	35.0	28.0	37.0
9	34.61685034054129	37.0	33.0	39.0	28.0	39.0
10	34.975856317388455	37.0	34.0	39.0	30.0	39.0
11	35.39344135342825	37.0	34.0	39.0	30.0	39.0
12	34.42521991223367	35.0	32.0	38.0	27.0	39.0
13	34.91491233295606	37.0	33.0	39.0	30.0	39.0
14	36.371952502928856	38.0	35.0	40.0	31.0	41.0
15	36.030841325628955	38.0	34.0	40.0	30.0	41.0
16	36.39896745497508	38.0	34.0	40.0	31.0	41.0
17	34.93861718393201	37.0	32.0	40.0	27.0	41.0
18	34.662472945334684	37.0	32.0	39.0	27.0	40.0
19	34.39018486527273	37.0	32.0	39.0	27.0	40.0
20	34.60171164194516	36.0	33.0	39.0	27.0	40.0
21	35.59846508210719	37.0	34.0	39.0	30.0	40.0
22	36.22086138083041	38.0	35.0	39.0	31.0	40.0
23	36.276630726157144	38.0	35.0	40.0	31.0	41.0
24	36.345572963205655	38.0	35.0	40.0	31.0	41.0
25	35.75614463573003	37.0	35.0	40.0	30.0	41.0
26	35.71043267607871	38.0	34.0	40.0	30.0	41.0
27	36.27905720696571	38.0	35.0	40.0	31.0	41.0
28	36.10065526895812	38.0	35.0	40.0	30.0	41.0
29	36.18629892178471	38.0	35.0	40.0	31.0	41.0
30	35.992577589801634	38.0	35.0	40.0	30.0	41.0
31	35.49580627866802	38.0	35.0	40.0	27.0	41.0
32	35.4989932686007	38.0	35.0	40.0	29.0	41.0
33	34.931512479895154	38.0	34.0	40.0	24.0	41.0
34	34.68340183872441	38.0	34.0	40.0	23.0	41.0
35	34.660074263815254	38.0	35.0	40.0	21.0	41.0
36	34.006812811500964	38.0	34.0	40.0	18.0	41.0
37	34.25983796985763	38.0	34.0	40.0	18.0	41.0
38	33.75334882150871	38.0	33.0	40.0	15.0	41.0
39	33.55829510931078	37.0	33.0	40.0	16.0	40.0
40	33.16310835765771	37.0	33.0	40.0	15.0	41.0
41	32.804298961497985	37.0	31.0	40.0	13.0	41.0
42	33.20123309703938	37.0	33.0	40.0	15.0	41.0
43	33.11517046921229	37.0	33.0	40.0	14.0	41.0
44	33.064426838227995	37.0	33.0	40.0	14.0	40.0
45	33.1402732272989	37.0	33.0	40.0	15.0	40.0
46	32.64614681995989	36.0	32.0	40.0	13.0	40.0
47	32.63601000774409	36.0	32.0	39.0	13.0	40.0
48	32.685572168940254	36.0	32.0	39.0	13.0	40.0
49	32.60377474633149	36.0	32.0	39.0	13.0	40.0
50	32.588207144417304	36.0	32.0	39.0	13.0	40.0
51	29.591971962431245	33.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	8.0
11	10.0
12	14.0
13	15.0
14	27.0
15	67.0
16	153.0
17	315.0
18	659.0
19	1151.0
20	1555.0
21	2085.0
22	3168.0
23	4564.0
24	6961.0
25	10473.0
26	14052.0
27	15459.0
28	14968.0
29	15449.0
30	17018.0
31	21059.0
32	27507.0
33	35651.0
34	45703.0
35	64666.0
36	80781.0
37	78091.0
38	38506.0
39	3465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.869045491551	13.125037231190802	17.661285518556024	24.34463175870217
2	31.557355890470802	36.52826592005719	19.40032962014257	12.514048569329441
3	21.96898393598221	34.741963026945456	31.714223307718274	11.574829729354065
4	20.24661940787514	17.556243918905505	48.76412303171105	13.43301364150831
5	29.38543714382161	20.420166398602095	31.491233295605724	18.703163161970572
6	17.578880482913366	36.997676773693925	34.28923174678819	11.134210996604516
7	61.562518615595394	3.573598617978197	31.164393081948333	3.6994896844780683
8	56.54454836083477	13.397470264688947	24.08530410436647	5.972677270109807
9	52.68322710033557	4.351978713687179	21.639959492464406	21.324834693512837
10	32.216993308313974	17.104306904152022	31.680467028057425	18.99823275947658
11	23.30771827406128	17.65850558964278	36.39582216397609	22.637953972319853
12	21.113560096106113	14.782271996187527	43.20148527630508	20.90268263140128
13	17.05188538750223	17.15037429757153	50.45451837731578	15.343221937610451
14	17.10807966482	24.53287265939914	36.74311471178095	21.61593296399992
15	13.595043783880383	18.782986835050934	50.829610214253094	16.79235916681559
16	15.272532316673617	22.813288060205316	34.30154286054685	27.612636762574216
17	16.518337602509877	19.585194892873453	40.058974206231014	23.837493298385656
18	16.833660967812396	22.786878735529477	38.43589285359703	21.9435674430611
19	16.942276761779947	23.5761799805405	33.83749329838566	25.644049959293895
20	18.222036893628005	23.083735430194	41.455491352435416	17.23873632374258
21	19.262921705287823	26.42858561188221	36.81658426163102	17.491908421198943
22	17.277853894878977	17.848930720200155	35.64960981712039	29.223605567800483
23	18.213697106888265	25.582891523202477	37.65274716546534	18.550664204443915
24	23.529516888068148	18.668811183256885	31.866523698893985	25.935148229780978
25	16.185143265622205	32.24499116379738	30.68505391076428	20.884811659816126
26	17.56716506820754	18.487718671193978	35.17225630944581	28.77285995115268
27	22.658604872818252	21.496594587081272	30.034351978713687	25.81044856138679
28	15.132145906554676	19.946387085244535	38.75220905065427	26.169257957546517
29	21.16359881654455	17.115823752506902	30.16441293858343	31.556164492365124
30	16.880721192986638	23.74972697126745	34.0372510474375	25.332300788308416
31	19.909652310319494	24.124223109151924	29.75218919401918	26.2139353865094
32	21.82800182681043	22.208256388872343	30.45809257163281	25.505649212684418
33	18.126725045173846	18.760548837394015	35.74412740017077	27.36859871726137
34	20.54268183713588	19.16919838764123	36.46055479438454	23.827564980838346
35	18.725799725978437	18.23494370643951	34.657373761442386	28.381882806139668
36	25.141677091400087	24.391294851174518	28.55860685848176	21.90842119894363
37	18.380095708981155	19.747225035245528	34.41591707869184	27.456762177081472
38	19.359822084549553	24.432000953118486	31.519429717440083	24.688747244891882
39	24.727864816028276	19.317130319096126	33.351005738567544	22.603999126308054
40	19.118166835448065	20.112189988284584	41.22793431425111	19.541708862016243
41	22.816465121820457	25.396040586962133	30.693393697504025	21.09410059371339
42	19.4765790989059	19.983717559222413	40.10583586505431	20.433867476817376
43	28.0590139195012	19.376898790730923	27.279243859335594	25.28484343043228
44	22.88616191100256	19.857032227318758	31.01229125712357	26.244514604555114
45	19.318520283552747	18.430531562121484	32.05873592660988	30.192212227715892
46	27.697821727130123	23.51085165107921	28.603284287444648	20.188042334346022
47	18.00142967772681	18.539743055141873	42.8579654891682	20.600861777963107
48	22.474136732789262	21.494807489922756	29.32050594706221	26.710549830225773
49	17.996068386251267	18.358451976728023	39.88622148090785	23.759258156112864
50	20.869919183495163	17.83781100454717	35.40855026707174	25.883719544885924
51	21.22455868628502	17.961914973888522	29.744047973630387	31.069478366196062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	311.0
2	534.0
3	9416.5
4	18299.0
5	12693.0
6	7087.0
7	6685.0
8	6283.0
9	6005.0
10	5727.0
11	5605.5
12	5484.0
13	5201.0
14	4918.0
15	4610.5
16	4303.0
17	3917.5
18	3532.0
19	3402.0
20	3272.0
21	2950.5
22	2629.0
23	2624.5
24	2620.0
25	2748.0
26	3056.0
27	3236.0
28	3626.0
29	4016.0
30	4511.0
31	5006.0
32	5434.5
33	5863.0
34	6621.5
35	7380.0
36	8117.0
37	8854.0
38	9697.5
39	10541.0
40	11930.5
41	13320.0
42	14976.0
43	16632.0
44	19853.0
45	23074.0
46	65280.0
47	107486.0
48	73666.5
49	39847.0
50	38078.5
51	36310.0
52	31132.0
53	25954.0
54	23879.0
55	21804.0
56	20488.0
57	19172.0
58	18342.0
59	17512.0
60	16888.5
61	16265.0
62	15100.0
63	13935.0
64	12843.0
65	11751.0
66	10481.5
67	9212.0
68	8067.0
69	6922.0
70	5799.5
71	4677.0
72	3882.0
73	3087.0
74	2485.0
75	1511.5
76	1140.0
77	831.0
78	522.0
79	399.0
80	276.0
81	202.5
82	129.0
83	90.5
84	52.0
85	45.0
86	38.0
87	35.5
88	33.0
89	25.0
90	17.0
91	13.0
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	503610.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.76604918855759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67909608946141	29.696926084505414
2	8.477745968161454	6.742529263560083
3	4.324383926531556	5.158909917979849
4	3.0533218503105264	4.856741875517843
5	2.3732681531049242	4.718774905700382
6	1.772519676000978	4.229166277412463
7	1.3043457420490916	3.6308073856046876
8	1.0204499169918941	3.246340927484734
9	0.7422942567239529	2.656629893274166
>10	2.1756234784303445	12.28212516754469
>50	0.04127832000625516	1.1403189858301532
>100	0.02649966222622906	1.8410872554228443
>500	0.0040768711117275475	0.9490161383759141
>1k	0.0020384355558637737	2.114058585423347
>5k	0.0010192177779318869	2.050831373129244
>10k+	0.0020384355558637737	14.685735963234196
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	25409	5.045372411191199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	21640	4.29697583447509	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	14314	2.8422787474434585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11105	2.205079327257203	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	5112	1.0150711860368142	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	5008	0.9944202855384128	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT	3612	0.7172216596175612	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC	3079	0.6113857945632534	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA	1917	0.38065169476380534	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT	1824	0.36218502412581166	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCCTTTGTCGTATGCCGTCTTC	653	0.12966382716784813	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT	641	0.1272810309564941	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCCTTTGTCGTATGC	632	0.1254939337979786	No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	581	0.11536704989972399	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATG	559	0.11099859017890829	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCC	555	0.1102043247751236	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGT	532	0.10563729870336172	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTAT	530	0.10524016600146939	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9713270189233735E-4	0.0	0.0	0.14793193145489567	0.0
2	3.9713270189233735E-4	0.0	0.0	1.503544409364389	0.0
3	3.9713270189233735E-4	0.0	0.0	1.7892813883759258	0.0
4	3.9713270189233735E-4	0.0	0.0	3.2378229185282263	0.0
5	3.9713270189233735E-4	0.0	0.0	10.025217926570164	0.0
6	3.9713270189233735E-4	0.0	0.0	10.986477631500566	0.0
7	3.9713270189233735E-4	0.0	0.0	12.264053533488216	0.0
8	3.9713270189233735E-4	0.0	0.0	13.540040904668295	0.0
9	3.9713270189233735E-4	0.0	0.0	13.862314092253927	0.0
10	3.9713270189233735E-4	0.0	0.0	19.382061515855522	0.0
11	3.9713270189233735E-4	0.0	0.0	20.09729751196362	0.0
12	3.9713270189233735E-4	0.0	0.0	24.046980798633864	0.0
13	3.9713270189233735E-4	0.0	0.0	24.46238160481325	0.0
14	3.9713270189233735E-4	0.0	0.0	24.713766605111097	0.0
15	3.9713270189233735E-4	0.0	0.0	25.369631262286294	0.0
16	5.95699052838506E-4	0.0	0.0	25.815809852862333	0.0
17	5.95699052838506E-4	0.0	0.0	26.245507436309843	0.0
18	7.942654037846747E-4	0.0	0.0	26.74311471178094	0.0
19	7.942654037846747E-4	0.0	0.0	27.172018029824667	0.0
20	9.928317547308434E-4	0.0	0.0	27.46569766287405	0.0
21	9.928317547308434E-4	0.0	0.0	27.779432497368997	0.0
22	9.928317547308434E-4	0.0	0.0	28.14876591012887	0.0
23	9.928317547308434E-4	0.0	0.0	28.46627350529179	0.0
24	9.928317547308434E-4	0.0	0.0	28.705545958181926	0.0
25	0.001191398105677012	0.0	0.0	28.90967216695459	0.0
26	0.0013899644566231807	0.0	0.0	29.12213816246699	0.0
27	0.0015885308075693494	0.0	0.0	29.374317428168624	0.0
28	0.0015885308075693494	0.0	0.0	29.592541847858463	0.0
29	0.0015885308075693494	0.0	0.0	29.80679494052938	0.0
30	0.0015885308075693494	0.0	0.0	30.086972061714423	0.0
31	0.001787097158515518	0.0	0.0	30.338754194714163	0.0
32	0.001787097158515518	0.0	0.0	30.556978614404002	0.0
33	0.001787097158515518	0.0	0.0	30.78413851988642	0.0
34	0.001787097158515518	0.0	0.0	31.006532832946128	0.0
35	0.001787097158515518	0.0	0.0	31.226941482496375	0.0
36	0.001787097158515518	0.0	0.0	31.45330712257501	0.0
37	0.001787097158515518	0.0	0.0	31.66200035741943	0.0
38	0.001787097158515518	0.0	0.0	31.87724628184508	0.0
39	0.0019856635094616867	0.0	0.0	32.10341335557276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAAA	30	2.1630622E-6	45.000004	21
CTAGACG	20	7.0295075E-4	45.0	1
CGCGGAT	20	7.0295075E-4	45.0	23
GCGCGAC	20	7.0295075E-4	45.0	9
CGACCCT	25	3.8876737E-5	45.0	12
CGATTTA	25	3.8876737E-5	45.0	10
ACTACCG	25	3.8876737E-5	45.0	26
GACGCTA	50	2.1827873E-11	45.0	40
TATCGCG	25	3.8876737E-5	45.0	1
AATACGC	25	3.8876737E-5	45.0	18
ACGAAAT	20	7.0295075E-4	45.0	36
CGTTTTT	6235	0.0	43.59262	1
GAATGAC	390	0.0	42.692307	1
TACGGCT	1815	0.0	42.272728	7
ATTGGGC	80	0.0	42.1875	4
TGATACC	2795	0.0	41.940968	4
GATACCT	2795	0.0	41.940968	5
ACGGCTG	1835	0.0	41.934605	8
GAATCTG	3520	0.0	41.484375	1
ACGGGAC	55	6.002665E-11	40.909092	5
>>END_MODULE