FastQCFastQC Report
Sat 18 Jun 2016
SRR3552084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581268
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC430107.3993407515982295No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG317835.467873683051535No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC208063.5794160352883693No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129222.2230709414590173No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT95201.6377987434367625No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC79621.3697640331138132TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT58671.009345086947845No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC51890.892703537782916No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTT37290.64152852040711No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA29560.5085433913444402No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT9630.16567228885815147No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGACCTATCGT8500.14623203066399665No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT8330.14330739005071672TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTAT8160.14038274943743678No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCC7690.13229697833013343No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATG7240.12455528258909833No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTT6480.11148041867090566No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC5950.10236242146479765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTAG207.0304045E-445.0000041
ACGGGAC253.8884187E-545.05
TATCGAG253.8884187E-545.01
CGTTTTT67950.043.841061
TACGGCT25900.043.0888027
ACGGCTG26150.042.934998
GAATCTG51200.042.495121
GATACCT40750.041.7423325
AATCTGT52200.041.6810342
TGATACC40650.041.678974
CGGCTGT27150.041.6022079
TGATCCG650.041.5384644
GAATGAT88200.040.6887781
ATACCTG42050.040.6658746
GAATGAC7800.040.3846171
AATGATA84550.040.156712
ATGATAC85000.040.076473
AATGACT7650.040.02
TACCTGT42800.039.953277
TACGGGA850.039.7058834
Produced by FastQC (version 0.11.5)