##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	581268
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.38160022571344	28.0	25.0	31.0	16.0	33.0
2	27.206613816690407	30.0	25.0	31.0	16.0	33.0
3	27.48297515087705	30.0	25.0	31.0	16.0	33.0
4	31.06623106725297	33.0	28.0	35.0	19.0	37.0
5	31.619046635975145	33.0	30.0	35.0	26.0	35.0
6	31.98650364375813	35.0	32.0	35.0	25.0	36.0
7	32.96480625116126	35.0	32.0	35.0	28.0	36.0
8	33.273042727278984	35.0	33.0	35.0	28.0	37.0
9	34.4306877378421	35.0	33.0	39.0	28.0	39.0
10	34.9250999538939	37.0	34.0	39.0	30.0	39.0
11	35.45729164516196	37.0	34.0	39.0	30.0	39.0
12	34.462941362676084	35.0	32.0	38.0	28.0	39.0
13	34.88980642319894	37.0	33.0	39.0	30.0	39.0
14	36.4144835084677	38.0	35.0	39.0	32.0	41.0
15	35.893744365765876	38.0	34.0	40.0	29.0	41.0
16	36.352169739259686	38.0	34.0	40.0	31.0	41.0
17	34.91073136659854	37.0	32.0	39.0	27.0	41.0
18	34.57397964450133	37.0	32.0	39.0	27.0	40.0
19	34.33774609990572	37.0	32.0	39.0	27.0	40.0
20	34.54117721945815	36.0	33.0	39.0	27.0	40.0
21	35.70722627084236	37.0	34.0	39.0	30.0	40.0
22	36.3631268192985	38.0	35.0	39.0	31.0	40.0
23	36.40130542193962	38.0	35.0	40.0	31.0	41.0
24	36.544146589869044	38.0	35.0	40.0	31.0	41.0
25	35.972437842785084	38.0	35.0	40.0	30.0	41.0
26	35.93364850636883	38.0	35.0	40.0	30.0	41.0
27	36.47501496727843	38.0	35.0	40.0	31.0	41.0
28	36.39656406339245	38.0	35.0	40.0	31.0	41.0
29	36.351665668848106	38.0	35.0	40.0	31.0	41.0
30	35.89927709765547	38.0	35.0	40.0	30.0	41.0
31	35.88724822285074	38.0	35.0	40.0	30.0	41.0
32	35.56955483529112	38.0	35.0	40.0	29.0	41.0
33	35.253266995602715	38.0	35.0	40.0	25.0	41.0
34	35.086168514351385	38.0	35.0	40.0	25.0	41.0
35	34.51074719406539	38.0	34.0	40.0	21.0	41.0
36	34.51892930627525	38.0	34.0	40.0	21.0	41.0
37	34.25208681709642	38.0	34.0	40.0	18.0	41.0
38	34.29667554381112	38.0	34.0	40.0	18.0	41.0
39	33.97245676693023	38.0	34.0	40.0	18.0	40.0
40	33.474233916197	37.0	33.0	40.0	15.0	41.0
41	33.27048280655395	37.0	33.0	40.0	15.0	41.0
42	33.44817536833268	37.0	33.0	40.0	15.0	41.0
43	33.53412883558015	37.0	33.0	40.0	15.0	41.0
44	33.523710233489545	37.0	33.0	40.0	15.0	41.0
45	33.582770770109484	37.0	33.0	40.0	17.0	40.0
46	33.086502267456666	37.0	33.0	40.0	15.0	40.0
47	32.9277734194898	37.0	32.0	39.0	15.0	40.0
48	33.105237171149966	37.0	32.0	40.0	15.0	40.0
49	33.00233111060647	37.0	32.0	40.0	15.0	40.0
50	33.0221051219059	36.0	32.0	40.0	15.0	40.0
51	29.59052622886517	33.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	5.0
11	5.0
12	10.0
13	12.0
14	46.0
15	70.0
16	183.0
17	448.0
18	745.0
19	1057.0
20	1578.0
21	2357.0
22	3540.0
23	5168.0
24	7445.0
25	11290.0
26	14803.0
27	16314.0
28	16140.0
29	15971.0
30	18370.0
31	22996.0
32	30829.0
33	40838.0
34	54422.0
35	79774.0
36	99822.0
37	90966.0
38	42371.0
39	3686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.07019137471872	11.552846535505138	15.974042954368725	22.40291913540742
2	28.712573202034175	41.44060915102844	17.670850623120486	12.175967023816897
3	19.867427761376852	40.02250252895394	29.477452741248445	10.632616968420763
4	18.77670885030657	16.124920002477342	51.99684138813766	13.101529759078428
5	31.579237116097907	18.746602255758102	29.945223201690098	19.72893742645389
6	16.99388233998775	37.64167303206094	34.40925700365408	10.955187624297226
7	58.827253521611375	3.136591038901161	33.98380781326342	4.052347626224048
8	51.40658009730451	15.648891733245248	25.69812891815823	7.2463992512920035
9	47.72824239421403	3.9991879821356067	22.22399994494794	26.04856967870242
10	30.709070514805564	15.629967588100497	33.27139976740505	20.389562129688887
11	22.141765932409836	16.886874901078333	37.19489116896165	23.77646799755018
12	20.675660796740917	13.263245181224494	44.61298402802149	21.44810999401309
13	16.226938348575874	16.579959674367075	52.606887012531224	14.586214964525828
14	16.399836220125657	25.886510181190086	35.362517805900204	22.351135792784053
15	12.249427114515163	18.537060357700753	53.5331378985253	15.680374629258791
16	13.429261545448915	24.679837871687415	32.49292925122319	29.397971331640484
17	14.269149514509657	20.361519987338024	40.62601072138841	24.743319776763904
18	14.8588946922934	23.935430816766104	38.41136962640297	22.79430486453753
19	15.033857016040795	24.809210209404267	32.676493459127286	27.480439315427653
20	15.853960651541113	24.633387697241204	42.7334723397813	16.779179311436376
21	17.516016708299787	28.13229009682281	37.06379845441346	17.28789474046395
22	14.996696876483826	17.554553149321826	35.56483412126592	31.883915852928425
23	16.63879656199894	26.8442439631977	38.51080740725448	18.006152067548875
24	24.461522051790226	17.888650329968275	30.319233124823665	27.330594493417838
25	14.709221907966722	35.24019901319185	28.97389844271489	21.07668063612654
26	18.2533702182126	17.50276980669846	35.094311057894124	29.149548917194824
27	24.105576085385742	21.08906734931219	29.617663453002745	25.18769311229932
28	17.72022543817998	18.04141979259137	38.65101811900879	25.587336650219868
29	30.748639181926407	17.129964147346836	29.714520668607253	22.406876002119507
30	19.06951698700083	22.53470000068815	40.16082082619377	18.234962186117247
31	29.670306984041783	18.403387077905546	27.44534362806829	24.480962309984378
32	23.8759057784017	29.90754694908373	28.758851338797246	17.45769593371732
33	16.799135682679932	19.138332060254477	27.235973767694077	36.826558489371514
34	28.168073934914705	18.096987964243688	27.94838181355244	25.78655628728917
35	16.57823929753573	20.466807049416104	39.398177776860244	23.55677587618792
36	28.23379232987194	26.560209748343276	26.20615619645327	18.999841725331517
37	16.833887294673026	25.62862569417205	38.866753373658966	18.670733637495957
38	21.737821452410934	21.661264683416256	25.4417927702884	31.159121093884405
39	25.146404068347135	23.405554752713034	27.097655470454246	24.350385708485586
40	18.659035075042837	18.973003846762595	39.82035825127136	22.54760282692321
41	17.281013233138587	32.436672928838334	26.40434360742377	23.87797023059931
42	18.975068298960203	19.4223662751089	43.37809753848483	18.224467887446067
43	26.29097077423839	22.772628116462627	26.03876353076378	24.897637578535203
44	22.977352959392224	18.617229918041247	30.43931542765127	27.966101694915253
45	18.87442625432675	16.801200134877543	31.5186798516347	32.80569375916101
46	29.33311312509892	23.97964450133157	27.34435750806857	19.342884865500938
47	16.229862989189154	17.0783528424066	45.690111962124185	21.001672206280062
48	22.59112836075614	20.860085193060687	27.996208289463727	28.55257815671945
49	16.722578913685254	16.82356503368498	42.288582891196484	24.16527316143328
50	20.260705905021435	16.492220455968678	36.20343800105975	27.043635637950135
51	20.720562632038924	16.14246784615702	29.056132455252996	34.080837066551055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	338.0
2	553.0
3	10608.5
4	20664.0
5	14085.0
6	7506.0
7	6980.5
8	6455.0
9	6269.0
10	6083.0
11	5862.5
12	5642.0
13	5283.0
14	4924.0
15	4582.0
16	4240.0
17	3919.5
18	3599.0
19	3488.5
20	3378.0
21	3148.5
22	2919.0
23	2909.5
24	2900.0
25	2886.0
26	3100.0
27	3328.0
28	3714.0
29	4100.0
30	4651.0
31	5202.0
32	5878.0
33	6554.0
34	7278.5
35	8003.0
36	8804.0
37	9605.0
38	10312.5
39	11020.0
40	12281.0
41	13542.0
42	15403.0
43	17264.0
44	21595.0
45	25926.0
46	91925.0
47	157924.0
48	102821.5
49	47719.0
50	45858.0
51	43997.0
52	36099.5
53	28202.0
54	25719.0
55	23236.0
56	21709.0
57	20182.0
58	18942.5
59	17703.0
60	16716.5
61	15730.0
62	14761.0
63	13792.0
64	12535.5
65	11279.0
66	9793.5
67	8308.0
68	7271.0
69	6234.0
70	5142.5
71	4051.0
72	3360.5
73	2670.0
74	2232.5
75	1334.0
76	873.0
77	723.0
78	573.0
79	417.0
80	261.0
81	204.0
82	147.0
83	126.0
84	105.0
85	74.0
86	43.0
87	30.5
88	18.0
89	14.0
90	10.0
91	9.0
92	8.0
93	6.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	581268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.68230509557882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4231573803579	29.175415850588614
2	8.94413815790449	6.919597620821396
3	4.588471944224651	5.324780150070049
4	3.1394038545399177	4.857575108781969
5	2.2511944073495416	4.354069444727786
6	1.5982269991130913	3.709386263501039
7	1.1060493488825918	2.99491768549699
8	0.8213665811467935	2.541788214978621
9	0.5320255351773033	1.8521976663330342
>10	1.5171863277865174	8.505861491902628
>50	0.03893697622156719	1.0667550713470764
>100	0.03078737654659555	2.2361400017622723
>500	0.0045275553744993455	1.2867262369163075
>1k	9.055110748998692E-4	1.170786020659523
>5k	0.0018110221497997384	4.998039111081746
>10k+	0.0018110221497997384	19.00596406103098
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	43010	7.3993407515982295	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	31783	5.467873683051535	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	20806	3.5794160352883693	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12922	2.2230709414590173	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	9520	1.6377987434367625	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	7962	1.3697640331138132	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT	5867	1.009345086947845	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC	5189	0.892703537782916	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTT	3729	0.64152852040711	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA	2956	0.5085433913444402	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT	963	0.16567228885815147	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGACCTATCGT	850	0.14623203066399665	No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	833	0.14330739005071672	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTAT	816	0.14038274943743678	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCC	769	0.13229697833013343	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATG	724	0.12455528258909833	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTT	648	0.11148041867090566	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC	595	0.10236242146479765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.440753662682274E-4	0.0	0.0	0.17926326582574648	0.0
2	3.440753662682274E-4	0.0	0.0	1.9226931467068546	0.0
3	3.440753662682274E-4	0.0	0.0	2.2523173475918163	0.0
4	3.440753662682274E-4	0.0	0.0	4.368552887824549	0.0
5	3.440753662682274E-4	0.0	0.0	13.622115788242256	0.0
6	3.440753662682274E-4	0.0	0.0	15.018545662241857	0.0
7	3.440753662682274E-4	0.0	0.0	16.742363247245677	0.0
8	3.440753662682274E-4	0.0	0.0	18.489577957155735	0.0
9	3.440753662682274E-4	0.0	0.0	18.91089824315118	0.0
10	3.440753662682274E-4	0.0	0.0	25.862941018600715	0.0
11	3.440753662682274E-4	0.0	0.0	26.752031765037813	0.0
12	3.440753662682274E-4	0.0	0.0	31.612784464309062	0.0
13	3.440753662682274E-4	0.0	0.0	32.121843968702905	0.0
14	3.440753662682274E-4	0.0	0.0	32.42067342430686	0.0
15	3.440753662682274E-4	0.0	0.0	33.19260650852963	0.0
16	3.440753662682274E-4	0.0	0.0	33.74381524529133	0.0
17	3.440753662682274E-4	0.0	0.0	34.28418560801558	0.0
18	3.440753662682274E-4	0.0	0.0	34.88304878300543	0.0
19	3.440753662682274E-4	0.0	0.0	35.419978392067	0.0
20	3.440753662682274E-4	0.0	0.0	35.78452624262818	0.0
21	3.440753662682274E-4	0.0	0.0	36.15819209039548	0.0
22	3.440753662682274E-4	0.0	0.0	36.63422035962757	0.0
23	3.440753662682274E-4	0.0	0.0	37.01304733788889	0.0
24	3.440753662682274E-4	0.0	0.0	37.30946826592897	0.0
25	3.440753662682274E-4	0.0	0.0	37.55204139914807	0.0
26	3.440753662682274E-4	0.0	0.0	37.822140561668625	0.0
27	3.440753662682274E-4	0.0	0.0	38.11322832153155	0.0
28	3.440753662682274E-4	0.0	0.0	38.34960809815782	0.0
29	3.440753662682274E-4	0.0	0.0	38.612309640303614	0.0
30	3.440753662682274E-4	0.0	0.0	38.94984757461274	0.0
31	3.440753662682274E-4	0.0	0.0	39.228376583606874	0.0
32	3.440753662682274E-4	0.0	0.0	39.50466910272026	0.0
33	3.440753662682274E-4	0.0	0.0	39.786466827693935	0.0
34	3.440753662682274E-4	0.0	0.0	40.06172712070852	0.0
35	3.440753662682274E-4	0.0	0.0	40.33887982823758	0.0
36	3.440753662682274E-4	0.0	0.0	40.615688460400364	0.0
37	3.440753662682274E-4	0.0	0.0	40.89215301719689	0.0
38	3.440753662682274E-4	0.0	0.0	41.16087587825237	0.0
39	3.440753662682274E-4	0.0	0.0	41.44095322639471	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCTAG	20	7.0304045E-4	45.000004	1
ACGGGAC	25	3.8884187E-5	45.0	5
TATCGAG	25	3.8884187E-5	45.0	1
CGTTTTT	6795	0.0	43.84106	1
TACGGCT	2590	0.0	43.088802	7
ACGGCTG	2615	0.0	42.93499	8
GAATCTG	5120	0.0	42.49512	1
GATACCT	4075	0.0	41.742332	5
AATCTGT	5220	0.0	41.681034	2
TGATACC	4065	0.0	41.67897	4
CGGCTGT	2715	0.0	41.602207	9
TGATCCG	65	0.0	41.538464	4
GAATGAT	8820	0.0	40.688778	1
ATACCTG	4205	0.0	40.665874	6
GAATGAC	780	0.0	40.384617	1
AATGATA	8455	0.0	40.15671	2
ATGATAC	8500	0.0	40.07647	3
AATGACT	765	0.0	40.0	2
TACCTGT	4280	0.0	39.95327	7
TACGGGA	85	0.0	39.705883	4
>>END_MODULE