FastQCFastQC Report
Sat 18 Jun 2016
SRR3552083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460078
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC195014.238629102021831No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG160733.4935380522433155No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC109522.3804659209960053No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103902.258312720886458No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT54781.1906676693951896No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC36010.7826933693851913No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT24760.5381696147175044No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAACAAT24460.5316489812596995No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC22230.4831789392233491No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTA21420.46557322888727565No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT20970.45579227870056815No Hit
GAATGCTGCCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT9190.19974873825742592No Hit
GAATCTTTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC8390.18236038236994595No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC7530.163667899790905No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAACAATCGT7490.16279848199653102No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATG6420.1395415559970266No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTAT6040.1312820869504736No Hit
GAACTGTCCCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT5170.11237224992283916No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT4670.10150452749316421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG302.162653E-645.0000042
CTATGCG207.02887E-445.01
TCGCACG253.887146E-545.035
CGAACAC207.02887E-445.034
CGCTATC207.02887E-445.026
TAACAGG351.2098099E-745.02
CGCTACG207.02887E-445.03
CGGTCTA253.887146E-545.031
CTCTACG253.887146E-545.01
CGTTTTT68850.044.1830061
TACGGCT12150.042.037047
GAATCTG23050.041.0954441
ACGGCTG12450.041.0240978
AAGGGCG1450.040.3448265
GATACCT19700.040.3172575
TGATACC20050.040.0623444
TTAACGG451.9239451E-840.0000042
AATCTGT23800.039.800422
CGTTAGG403.4521872E-739.3752
CGACCAC10300.039.1019412
Produced by FastQC (version 0.11.5)